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Daglb (NM_144915) Mouse Tagged ORF Clone
CAT＃: MG209911
- TrueORF®
Daglb (tGFP-tagged) - Mouse diacylglycerol lipase, beta (Daglb)
ORF Plasmid: DDK
"NM_144915" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | E330036I19Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209911 representing NM_144915
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGGATGGTGCTGTTCGGCCGGCGCTGGTCTCTAGCCAGCGACGACTTGGTGTTCCCCGGGTCTT TTGAGCTGTTCCTGCGTGTGCTGTGGTGGATTGTCAGTCTGACGTTGTATCTCACGCACAGAAGGAGGCT GGACTGTCCCGGTGGAGTCTTGCTCAGCACGTACCTGATAGTCCTCCTTGTTCTCCTGGCAGTTATTATA TGCACCGTGTTGGCCATCGTGTGTGTCAGCATGAGAGGAACCATTTGTAACCCTGGACCTCGGAAGTCTA TGTCTAAGCTGCTCTATATCCGCCTGGCGCTCTTCCTGCCAGAGATGGTCTGGGCTTCTCTGGGGGCCGC CTGGGTGGCAAAAGGTATCCAGTGTGACAGGACAGTTGTCATTGGCATCATCGCCACCGTCATTGTCAGC TGGATTGTCATTGCTGCCACCATGGTCACCATCATCTTTGTCTTTGACCCGCTGGGCGGGAAGATGGCTC CGTATCCCCCATGCATCCCGGAGCACCTGGACAGTAACAGCTCAAACCGGTTACTGACTGGCCTGAAGAC GGCTGCGAAGAGCGTGTGGGAGACCCGGGTGCAGTTCTGCTGCTGCTGCGTTGGGCAGGATGATAACACC AGGGTGGCGTTTTCCAGCACTGCCGACCTGTTCTCCACCTACTTCTCTGACACAGACCTGGTGCCTAGTG ATATCGCAGCAGGCTTCACCCTTCTCCACCAGCAACAAGACAATATCAGCCACAGCCGGGAGCCTCCGGA GGTCGTGACCCACACACCAGGACAGCCTCAGGAAACTGAGTTAGATGCAGAAGTGGAGAACTGCCATCAT TACATGCCGTTTGCAGCAGCTGCCTATGGGTGGCCGCTCTACATCTACAGGAACCCGTTCACGGGGCTGT GCAGGATTGGTGGCGACTGTTGCAGAGCCAGAGACATAGAGTACGATGCAGTGGAAGGTGACCAGCACAA CTGCCACTTCGCCTCCATCCTGAAGACCACAGGGCTGCAGTACAGGGATTTCATTCACATAAGCTTTCAC GACAAGGTGTATGAGCTGCCCTTCATAGTGGTTCTGGACCACAGGAAGGAGTCTGTTGTGGTCGCCGTGA GAGGGACCATGTCTCTCCAGGACGTCCTGACCGACCTGTCTGCCGAGAGTGAGACCCTGGAGCTGGGGAT TGAGCTACAGGACTGTGTGGCCCACAAGGGAATTGCTCAAGCGGCCAGATACATTCACCGCAGACTGGTC AATGATGGGATTCTGAGCCAAGCCTTCAGTGTCGCTCCGGAGTACCAGCTCGTTCTGGTGGGGCACAGCC TAGGAGCCGGCGCTGCGGCCCTGTTAGCCATCATGCTCCGGGGGGCCTACCCACAAGTCCGTGCTTATGC CTTCTCCCCGCCCAGGGGGCTGCTCAGCAAATCCCTTTACGAGTACTCCAAGGACTTTGTCGTGTCGCTT ATCCTAGGGATGGATGTGATCCCCAGGTTAAGTGTGACCAACATGGAGGACCTCAAGAGGAGGATCCTGA GAGTGATCGCTAACTGCAATAAGCCGAAGTACAAGATCTTGCTGCATGGCTGTTGGTACGGACTGTTCGG AGGAAGCCCTGATAACTTTCCAACTGAACTGGATGAGGGCACCCAGGGGGCCCTGACTCAGCCTCTTCTT GGAGAGCAGACACTTCTAACCCGATACTCCCCGGGCTACTGTTCCAGCGACTCCCCACTGGACTCCCCCA CCAAGTACCCCACTCTGTACCCACCTGGCAGGATCATCCACCTGGAAGAGGAGGGTGGTTCAGGGAGGTT TGGCTGCTGTTCTGCTGCCCAGTACAGAGCGAGGTGGGCACACGAAGCAGAATTCAGTAAGATCCTGATA GGCCCAAAGATGCTGATTGACCACATGCCTGACGTCATGATTCGGGCCCTGGACAGAGTGCTTGCCGACA GGACAGCCTGCGTCTCCTGCCCAGGGCAAGGCGGCTCAAGTGTACCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209911 representing NM_144915
Red=Cloning site Green=Tags(s) MPGMVLFGRRWSLASDDLVFPGSFELFLRVLWWIVSLTLYLTHRRRLDCPGGVLLSTYLIVLLVLLAVII CTVLAIVCVSMRGTICNPGPRKSMSKLLYIRLALFLPEMVWASLGAAWVAKGIQCDRTVVIGIIATVIVS WIVIAATMVTIIFVFDPLGGKMAPYPPCIPEHLDSNSSNRLLTGLKTAAKSVWETRVQFCCCCVGQDDNT RVAFSSTADLFSTYFSDTDLVPSDIAAGFTLLHQQQDNISHSREPPEVVTHTPGQPQETELDAEVENCHH YMPFAAAAYGWPLYIYRNPFTGLCRIGGDCCRARDIEYDAVEGDQHNCHFASILKTTGLQYRDFIHISFH DKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESETLELGIELQDCVAHKGIAQAARYIHRRLV NDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIMLRGAYPQVRAYAFSPPRGLLSKSLYEYSKDFVVSL ILGMDVIPRLSVTNMEDLKRRILRVIANCNKPKYKILLHGCWYGLFGGSPDNFPTELDEGTQGALTQPLL GEQTLLTRYSPGYCSSDSPLDSPTKYPTLYPPGRIIHLEEEGGSGRFGCCSAAQYRARWAHEAEFSKILI GPKMLIDHMPDVMIRALDRVLADRTACVSCPGQGGSSVP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144915 |
| ORF Size | 2007 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144915.3, NP_659164.2 |
| RefSeq Size | 3257 bp |
| RefSeq ORF | 2010 bp |
| Locus ID | 231871 |
| UniProt ID | Q91WC9 |
| Gene Summary | Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220283 | Daglb (untagged) - Mouse diacylglycerol lipase, beta (Daglb), (10ug) |
CNY 8,550.00 |
|
| MR209911 | Daglb (Myc-DDK-tagged) - Mouse diacylglycerol lipase, beta (Daglb) |
CNY 4,904.00 |
|
| MR209911L3 | Lenti ORF clone of Daglb (Myc-DDK-tagged) - Mouse diacylglycerol lipase, beta (Daglb) |
CNY 7,700.00 |
|
| MR209911L4 | Lenti ORF clone of Daglb (mGFP-tagged) - Mouse diacylglycerol lipase, beta (Daglb) |
CNY 7,700.00 |
