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Wwc1 (BC037006) Mouse Tagged ORF Clone
CAT＃: MG209954
- TrueORF®
Wwc1 (tGFP-tagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674)
ORF Plasmid: DDK
"BC037006" in other vectors (4)
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CNY 4,280.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC47054, mKIAA0869, RP23-196B5.5, Kibra |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209954 representing BC037006
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCCCTGTCTTCAGGCAGTAGCCCTGGGTCACTCACCTCCAGCCGGGGCTCCCTGGCCGCCTCTA GCCTGGACTCCTCCACCTCAGCCAGCTTCACTGACCTCTATTATGACCCGTTCGAGCAGTTGGACTCGGA GCTCCAGAGCAAGGTGGAGCTGCTGTTCCTGGAGGGTGCCACGGGCTTCCGGCCCTCAGGCTGCATCACC ACCATCCACGAGGATGAGGTGGCCAAGACCCAGAAGGCAGAGGGAGGCAGCCGCCTGCAGGCCCTGCGCT CCCTATCTGGCACTCCAAGGTCCATGACCTCCCTGTCTCCACGCTCATCTCTCTCCTCCCCATCCCCTCC CTGTTCCCCTCTCATCACTGATCCCCTCCTGACTGGAGATGCCTTCCTGGCCCCGCTGGAGTTTGAAGAC ACAGAGCTGAGTACCACTCTCTGTGAGCTGAACCTCGGCGGTAGCGGCACCCAGGAAAGATACCGGCTGG AGGAACCAGGACCTGAGGGCAAGCCACTGGGCCAAGCTGCAAGTGTGGCCCCAGGATGTGGCCTCAAAGT AGCCTGTGTGTCCGCTGCAGTGTCCGACGAGTCAGTAGCCGGGGACAGTGGCGTGTATGAGGCTTCAGCA CAGAGACCAGGTACTTCTGAAGCTGCCGCATTCGACAGTGATGAATCGGAAGCTGTGGGTGCCACTCGGG TTCAGATTGCCCTGAAATATGATGAGAAGAATAAGCAATTTGCAATATTAATCATTCAGCTGAGTCACCT CTCTGCTCTGTCACTACAACAAGACCAGAAAGTGAACATCCGTGTAGCCATCCTGCCTTGCTCTGAAAGC AGCACCTGCTTGTTCAGAACCCGGCCTCTGGACTCGGCAAATACCCTCGTGTTCAATGAGGCATTCTGGG TGTCCATCTCCTACCCAGCCCTTCACCAAAAGACCTTACGAGTCGATGTCTGCACCACTGACAGGAGCCA TACAGAGGAGTGTCTGGGAGGTGCCCAGATCAGCCTGGCCGAGGTCTGCCGGTCTGGGGAGAGGTCAACG CGCTGGTACAATCTCCTGAGCTACAAATACTTGAAGAAGCAGTGCAGGGAGCCCCAGCCAACAGAAGCTC CAGGGCCAGACCATGTGGACGCTGTGTCTGCCTTGCTAGAACAGACAGCCGTGGAGCTAGAGAAGAGGCA GGAGGGAAGAAGCAGCTCCCAGACGCTGGAGGGCAGCTGGACGTACGAGGAAGAGGCCAGCGAGAATGAG GCAGTCGCTGAGGAGGAAGAGGAGGGAGAGGAAGATGTCTTCACTGAAAAGGTCTCTCCCGAGGCAGAGG AGTGCCCAGCATTAAAGGTGGACAGAGAGACCAATACTGACAGCGTGGCCCCATCCCCCACAGTGGTGCG CCCTAAGGACCGGAGGGTGGGTGCCCCGTCGACAGGGCCATTTCTTCGAGGGAATACCATCATCCGCTCC AAGACCTTCTCGCCTGGACCCCAGAGCCAGTATGTGTGTCGGCTAAACCGCAGTGACAGTGATAGTTCTA CGCTGTCCAAGAAGCCACCTTTTGTGCGAAACTCCCTGGAGCGGCGCAGCGTCAGGATGAAGCGGCCTTC CTCCGTCAAGTCCCTGCGCACAGAGCGTCTGATCCGCACCTCGCTGGACCTAGAACTCGACCTGCAGGCC ACGAGGACCTGGCACAGCCAGCTGACTCAGGAGATCTCAGTGCTGAAGGAGCTGAAGGAGCATTTGGAGC AAGCCAAGAACCACGGGGAGAAGGAGCTGCCACAGTGGCTGCGTGAAGACGAGCGCTTCCGCTTGCTACT GAGGATGCTGGAGAAGAAGGTGGATCGTGGGGAGCACAAGAGTGAGTTGCAAGCCGACAAGATGATGAGA GCTGCTGCCAAGGATGTGCACAGGCTTCGAGGCCAGAGCTGTAAGGAGCCCCCAGAAGTGCAGTCTTTCA GGGAGAAAATGGCATTTTTCACCCGGCCTCGGATGAACATCCCAGCTCTCTCTGCAGATGACGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209954 representing BC037006
Red=Cloning site Green=Tags(s) MQSLSSGSSPGSLTSSRGSLAASSLDSSTSASFTDLYYDPFEQLDSELQSKVELLFLEGATGFRPSGCIT TIHEDEVAKTQKAEGGSRLQALRSLSGTPRSMTSLSPRSSLSSPSPPCSPLITDPLLTGDAFLAPLEFED TELSTTLCELNLGGSGTQERYRLEEPGPEGKPLGQAASVAPGCGLKVACVSAAVSDESVAGDSGVYEASA QRPGTSEAAAFDSDESEAVGATRVQIALKYDEKNKQFAILIIQLSHLSALSLQQDQKVNIRVAILPCSES STCLFRTRPLDSANTLVFNEAFWVSISYPALHQKTLRVDVCTTDRSHTEECLGGAQISLAEVCRSGERST RWYNLLSYKYLKKQCREPQPTEAPGPDHVDAVSALLEQTAVELEKRQEGRSSSQTLEGSWTYEEEASENE AVAEEEEEGEEDVFTEKVSPEAEECPALKVDRETNTDSVAPSPTVVRPKDRRVGAPSTGPFLRGNTIIRS KTFSPGPQSQYVCRLNRSDSDSSTLSKKPPFVRNSLERRSVRMKRPSSVKSLRTERLIRTSLDLELDLQA TRTWHSQLTQEISVLKELKEHLEQAKNHGEKELPQWLREDERFRLLLRMLEKKVDRGEHKSELQADKMMR AAAKDVHRLRGQSCKEPPEVQSFREKMAFFTRPRMNIPALSADDV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC037006 |
| ORF Size | 2027 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC037006, AAH37006 |
| RefSeq Size | 2583 bp |
| RefSeq ORF | 2027 bp |
| Locus ID | 211652 |
| Gene Summary | Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ and may be associated with memory performance (By similarity). Regulates collagen-stimulated activation of the ERK/MAPK cascade.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220895 | Wwc1 (untagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674), (10ug) |
CNY 9,120.00 |
|
| MR209954 | Wwc1 (Myc-DDK-tagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674) |
CNY 4,944.00 |
|
| MR209954L3 | Lenti ORF clone of Wwc1 (Myc-DDK-tagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674) |
CNY 5,800.00 |
|
| MR209954L4 | Lenti ORF clone of Wwc1 (mGFP-tagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674) |
CNY 5,800.00 |
