Wwc1 (BC037006) Mouse Tagged ORF Clone

CAT#: MG209954

  • TrueORF®

Wwc1 (tGFP-tagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674)

ORF Plasmid: DDK tGFP



  "BC037006" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,280.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MGC47054, mKIAA0869, RP23-196B5.5, Kibra
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209954 representing BC037006
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGTCCCTGTCTTCAGGCAGTAGCCCTGGGTCACTCACCTCCAGCCGGGGCTCCCTGGCCGCCTCTA
GCCTGGACTCCTCCACCTCAGCCAGCTTCACTGACCTCTATTATGACCCGTTCGAGCAGTTGGACTCGGA
GCTCCAGAGCAAGGTGGAGCTGCTGTTCCTGGAGGGTGCCACGGGCTTCCGGCCCTCAGGCTGCATCACC
ACCATCCACGAGGATGAGGTGGCCAAGACCCAGAAGGCAGAGGGAGGCAGCCGCCTGCAGGCCCTGCGCT
CCCTATCTGGCACTCCAAGGTCCATGACCTCCCTGTCTCCACGCTCATCTCTCTCCTCCCCATCCCCTCC
CTGTTCCCCTCTCATCACTGATCCCCTCCTGACTGGAGATGCCTTCCTGGCCCCGCTGGAGTTTGAAGAC
ACAGAGCTGAGTACCACTCTCTGTGAGCTGAACCTCGGCGGTAGCGGCACCCAGGAAAGATACCGGCTGG
AGGAACCAGGACCTGAGGGCAAGCCACTGGGCCAAGCTGCAAGTGTGGCCCCAGGATGTGGCCTCAAAGT
AGCCTGTGTGTCCGCTGCAGTGTCCGACGAGTCAGTAGCCGGGGACAGTGGCGTGTATGAGGCTTCAGCA
CAGAGACCAGGTACTTCTGAAGCTGCCGCATTCGACAGTGATGAATCGGAAGCTGTGGGTGCCACTCGGG
TTCAGATTGCCCTGAAATATGATGAGAAGAATAAGCAATTTGCAATATTAATCATTCAGCTGAGTCACCT
CTCTGCTCTGTCACTACAACAAGACCAGAAAGTGAACATCCGTGTAGCCATCCTGCCTTGCTCTGAAAGC
AGCACCTGCTTGTTCAGAACCCGGCCTCTGGACTCGGCAAATACCCTCGTGTTCAATGAGGCATTCTGGG
TGTCCATCTCCTACCCAGCCCTTCACCAAAAGACCTTACGAGTCGATGTCTGCACCACTGACAGGAGCCA
TACAGAGGAGTGTCTGGGAGGTGCCCAGATCAGCCTGGCCGAGGTCTGCCGGTCTGGGGAGAGGTCAACG
CGCTGGTACAATCTCCTGAGCTACAAATACTTGAAGAAGCAGTGCAGGGAGCCCCAGCCAACAGAAGCTC
CAGGGCCAGACCATGTGGACGCTGTGTCTGCCTTGCTAGAACAGACAGCCGTGGAGCTAGAGAAGAGGCA
GGAGGGAAGAAGCAGCTCCCAGACGCTGGAGGGCAGCTGGACGTACGAGGAAGAGGCCAGCGAGAATGAG
GCAGTCGCTGAGGAGGAAGAGGAGGGAGAGGAAGATGTCTTCACTGAAAAGGTCTCTCCCGAGGCAGAGG
AGTGCCCAGCATTAAAGGTGGACAGAGAGACCAATACTGACAGCGTGGCCCCATCCCCCACAGTGGTGCG
CCCTAAGGACCGGAGGGTGGGTGCCCCGTCGACAGGGCCATTTCTTCGAGGGAATACCATCATCCGCTCC
AAGACCTTCTCGCCTGGACCCCAGAGCCAGTATGTGTGTCGGCTAAACCGCAGTGACAGTGATAGTTCTA
CGCTGTCCAAGAAGCCACCTTTTGTGCGAAACTCCCTGGAGCGGCGCAGCGTCAGGATGAAGCGGCCTTC
CTCCGTCAAGTCCCTGCGCACAGAGCGTCTGATCCGCACCTCGCTGGACCTAGAACTCGACCTGCAGGCC
ACGAGGACCTGGCACAGCCAGCTGACTCAGGAGATCTCAGTGCTGAAGGAGCTGAAGGAGCATTTGGAGC
AAGCCAAGAACCACGGGGAGAAGGAGCTGCCACAGTGGCTGCGTGAAGACGAGCGCTTCCGCTTGCTACT
GAGGATGCTGGAGAAGAAGGTGGATCGTGGGGAGCACAAGAGTGAGTTGCAAGCCGACAAGATGATGAGA
GCTGCTGCCAAGGATGTGCACAGGCTTCGAGGCCAGAGCTGTAAGGAGCCCCCAGAAGTGCAGTCTTTCA
GGGAGAAAATGGCATTTTTCACCCGGCCTCGGATGAACATCCCAGCTCTCTCTGCAGATGACGTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209954 representing BC037006
Red=Cloning site Green=Tags(s)

MQSLSSGSSPGSLTSSRGSLAASSLDSSTSASFTDLYYDPFEQLDSELQSKVELLFLEGATGFRPSGCIT
TIHEDEVAKTQKAEGGSRLQALRSLSGTPRSMTSLSPRSSLSSPSPPCSPLITDPLLTGDAFLAPLEFED
TELSTTLCELNLGGSGTQERYRLEEPGPEGKPLGQAASVAPGCGLKVACVSAAVSDESVAGDSGVYEASA
QRPGTSEAAAFDSDESEAVGATRVQIALKYDEKNKQFAILIIQLSHLSALSLQQDQKVNIRVAILPCSES
STCLFRTRPLDSANTLVFNEAFWVSISYPALHQKTLRVDVCTTDRSHTEECLGGAQISLAEVCRSGERST
RWYNLLSYKYLKKQCREPQPTEAPGPDHVDAVSALLEQTAVELEKRQEGRSSSQTLEGSWTYEEEASENE
AVAEEEEEGEEDVFTEKVSPEAEECPALKVDRETNTDSVAPSPTVVRPKDRRVGAPSTGPFLRGNTIIRS
KTFSPGPQSQYVCRLNRSDSDSSTLSKKPPFVRNSLERRSVRMKRPSSVKSLRTERLIRTSLDLELDLQA
TRTWHSQLTQEISVLKELKEHLEQAKNHGEKELPQWLREDERFRLLLRMLEKKVDRGEHKSELQADKMMR
AAAKDVHRLRGQSCKEPPEVQSFREKMAFFTRPRMNIPALSADDV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC037006
ORF Size 2027 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC037006, AAH37006
RefSeq Size 2583 bp
RefSeq ORF 2027 bp
Locus ID 211652
Gene Summary Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ and may be associated with memory performance (By similarity). Regulates collagen-stimulated activation of the ERK/MAPK cascade.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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