Tcf25 (BC025071) Mouse Tagged ORF Clone

CAT#: MG209961

  • TrueORF®

Tcf25 (tGFP-tagged) - Mouse transcription factor 25 (basic helix-loop-helix) (cDNA clone MGC:36794 IMAGE:3498003)



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Mouse Protein / Lysate Request

CNY 7,410.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Nulp1, mKIAA1049
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209961 representing BC025071
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCGCCGGGCCCTCCGGAGACTGAGGGGGGAACAGCGCGGCCAGGAACCCCTAGGGCCCGACGCCT
TGAAGTTTGTCCTCCTTGATGACGATGACGCAGAGGAAGAGGGACCAAAACCGGGGCTGGGTGGCCGGCG
CCCCGGGGGTGCAGGGAAGGAAGGAGTCCGTGTCAACAACCGGTTTGAGCTGATAAACACGGAGGATCTT
GAGGATGACCTTGTGGTAAATGGGGAGAGATCTGACTGTACCCTGCCGGACTCTGTGTCATCAGGGAACA
AAGGAAGGGCTAAACATGGAAACGCGGAGACCAAGCAAGATGGAGGGGCCACCAAAGCAGGGTCCTCGGA
ACAGTCTAATGCAAGTGGCAAACTCCGAAAGAAGAAAAAGAAGCAGAAAAATAAGAAAAGCTGCACAGGA
GAATCCTCGGAGAATGGACTAGAAGACATTGATCGCATCTTAGAGCGGATTGAGGACAGCAGTGGGTTCA
GCCACCCCGGCCCCCCTCCACTGAGCTCCAGGAAGCACGTCCTATATGTGGAGCACAGACACCTGAATCC
AGACACGGAACTGAAGAGGTATTTTGGTGCTCGAGCGGTCCTGGGCGAGCAAAGGCCTCGACAGAGGCAG
CGTGTGTATCCCAAGTGTACATGGCTGACGACCCCTAAGAGCACCTGGCCCCGGTACAGCAAACCAGGTC
TATCAATGCGGCTGCTGGAGTCCAAGAAAGGCCTTTCTTTCTTTGCATTTGACCATAATGAAGAATACCA
ACAAGCACAGCACAAGTTCCTGGTGGCTGTGGAGTCCATGGAGCCAAATAACATTGTGGTTCTGCTCCAG
ACAAGCCCCTATCATGTGGACTCCCTGCTGCAGCTCAGTGATGCCTGCCGCTTTCAAGAGGATCAAGAGA
TGGCTCGAGACCTTATAGAGAGAGCCCTGTACAGCATGGAGTGTGCATTCCACCCTTTGTTCAGTCTCAC
CAGTGGAACTTGCAGGCTGGATTATCGCAGACCTGAGAATAGGAGCTTCTACCTGACCCTCTACAAGCAG
ATGAGTTTCTTGGAGAAACGTGGATGCCCACGTACTGCGCTGGAGTACTGCAAGCTCATCCTGAGCCTTG
AGCCAGATGAGGACCCCCTGTGCATGTTGCTGCTTATTGACCACCTGGCCTTACGGGCCCGGAACTATGA
ATACCTGATTCGCCTCTTCCAGGAATGGGAGGCTCATCGGAACCTGTCCCAGCTCCCAAATTTTGCCTTC
TCAGTTCCACTGGCCTATTTCCTTCTGAGTCAGCAAACAGACTTACCTGAGCATGAGCTCAGCTCTGCCA
GGCAACAGGCTTCCCTCCTGATACAGCAGGCACTCACCATGTTCCCTGGAGTTCTTATGCCACTGCTGGA
GTATTGCAGTGTGCGACCTGATGCCACGGTCTCAAACCACCGCTTCTTTGGGCCGGATGCTGAGATCAGC
CAGCCTCCCGCCTTGGGCCAGCTGGTGAGTCTGTACCTGGGGAGGTCACACTTTCTTTGGAAAGAACCCG
CCATTATGAGCTGGCTAGAGGAAAATGTCCATGAGGTTCTACAAGCGGTGGATGCTGGAGACCCTGCTGT
GGAGGTCTGTGAGAACAGGCGGAAAGTGTTGTACCAGCGTGCGCCCAGAAACATCCACCGCCACGTGATA
CTGTCTGAGATCAAGGAGGCTGTTGCTGCCCTACCCTCGGATGTGACCACACAGTCTGTGATGGGGTTCG
ACCCTCTGCCTCCCCTGGACACCATCTACTCTTACGTCAGACCAGAGAGGCTGAGTCCAGTCAGCCACGG
AAACACAATCGCCCTCTTCTTCCGGTCCTTGTTGCCAAATTATACCACAGAGGGGGAGAGGCTGGAGGAA
GGAGTGGCCGGGGGTCCGAACCGCAATCAAGGCTTGAACAGGCTGATGTTGGCCGTACGTGACATGATGG
CCAACTTCCACTTCAACGACCTGGAGGTGCCTCGGGAGGACAATCCTGAGGGCGAGGGGGACTGGGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209961 representing BC025071
Red=Cloning site Green=Tags(s)

MSRRALRRLRGEQRGQEPLGPDALKFVLLDDDDAEEEGPKPGLGGRRPGGAGKEGVRVNNRFELINTEDL
EDDLVVNGERSDCTLPDSVSSGNKGRAKHGNAETKQDGGATKAGSSEQSNASGKLRKKKKKQKNKKSCTG
ESSENGLEDIDRILERIEDSSGFSHPGPPPLSSRKHVLYVEHRHLNPDTELKRYFGARAVLGEQRPRQRQ
RVYPKCTWLTTPKSTWPRYSKPGLSMRLLESKKGLSFFAFDHNEEYQQAQHKFLVAVESMEPNNIVVLLQ
TSPYHVDSLLQLSDACRFQEDQEMARDLIERALYSMECAFHPLFSLTSGTCRLDYRRPENRSFYLTLYKQ
MSFLEKRGCPRTALEYCKLILSLEPDEDPLCMLLLIDHLALRARNYEYLIRLFQEWEAHRNLSQLPNFAF
SVPLAYFLLSQQTDLPEHELSSARQQASLLIQQALTMFPGVLMPLLEYCSVRPDATVSNHRFFGPDAEIS
QPPALGQLVSLYLGRSHFLWKEPAIMSWLEENVHEVLQAVDAGDPAVEVCENRRKVLYQRAPRNIHRHVI
LSEIKEAVAALPSDVTTQSVMGFDPLPPLDTIYSYVRPERLSPVSHGNTIALFFRSLLPNYTTEGERLEE
GVAGGPNRNQGLNRLMLAVRDMMANFHFNDLEVPREDNPEGEGDWD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC025071
ORF Size 2030 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC025071, AAH25071
RefSeq Size 2167 bp
RefSeq ORF 2030 bp
Locus ID 66855
Gene Summary May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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