Uvrag (NM_178635) Mouse Tagged ORF Clone
CAT#: MG210112
- TrueORF®
Uvrag (tGFP-tagged) - Mouse UV radiation resistance associated gene (Uvrag)
ORF Plasmid: DDK
"NM_178635" in other vectors (4)
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CNY 7,600.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 9530039D02Rik; AI648770; BB124205; Uvrag1; Uvragl |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210112 representing NM_178635
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCCTGCGCCTCGCTCGGGGGCCCGGTGCCCCTGCCACCCCCGGGCCCGTCCGCCGCGCTGACGT CCGGTGCCCCCGCGCGGGCCCTGCATGTGGAGCTGCCGTCTCAGCAGCGGCGTCTCCGACATCTCCGGAA CATCGCTGCTCGGAACATTGTTAATAGAAATGGCCATCAGCTTCTGGATACCTACTTCACTCTTCATTTG TGTGATAATGAGAAAATCTTTAAAGAATTTTATAGGAGTGAAGTGATTAAGAATTCCTTGAATCCGACGT GGAGGAGTCTTGACTTTGGAATAATGCCGGATCGTCTGGATACATCTGTGTCCTGCTTTGTGGTGAAGAT TTGGGGTGGGAAGGAAGAGGCCTTTCAGCTGTTGATAGAGTGGAAAGTCTACCTGGATGGGCTGAAGTAC TTGGGACAGCAGATCCATGCCCGCAACCAGAATGAAATCATTTTTGGGCTGAATGATGGCTACTACGGTG CTCCCTGTGAACACAAGGGTCATCCAAACGCACAGAAGAACCTCCTTCAGGTGGACCAGAACTGTGTTCG CAATTCTTACGATGTCTTCTCTTTGCTGCGGCTTCATAGAGCCCAGTGTGCAATTAAACAGACTCAGGTA ACTGTTCAGAGACTTGGAAAGGAAATTGAAGAAAAACTAAGACTCACATCGACAAGCAATGAGCTGAAAA AAGAAAGTGAATGTCTGCGATTGAAAATTTTGGTGCTTCGAAATGAACTGGAAAGACAGAAGAAAGCGCT GGGGCGGGAGGTGGCCTTCCTGCATAAGCAACAGATGGCTCTACAGGACAAAGGAAGTGCATTTTCCACT GAGCATGGCAAGCTCCAGCTGCAGAAGGACTCCCTGAGTGAGCTGAGGAAGGAGTGTACTGCCAAAAGGG AACTCTTTCTGAAGACTAATGCTCAGTTGACCATCCGCTGCAGGCAGCTACTCTCAGAGCTGTCCTACAT TTACCCTATTGATTTGAATGAGCACAAGGATTATTTTGTATGTGGTGTCAAGTTGCCTAATTCCGAGGAC TTCCAAGCAAAAGAGGATGGAAGCATTGCCGTCGCCCTTGGTTACACTGCACATTTGGTCTCCATGATCT CCTTCTTCCTACAAGTACCCCTCAGGTATCCCATAATTCACAAGGGGTCCAGATCAACAATCAAAGATAA CATCAACGACAAGCTGACAGAAAAGGAGCGAGAGTTTCCACTGTATCCAAAGGGAGGGGAGAAGCTGCAG TTTGACTATGGTGTCTACCTTCTGAACAAGAACATAGCACAGCTGAGATACCAGCATGGCCTGGGGACTC CAGACTTGAGGCAAACTCTTCCTAACCTGAAAAACTTCATGGAGCACGGACTGATGGTCAGGTGTGACAG ACATCACATCTCCAATGCCATCCCTGTCCCGAAGAGACAAAGCTCCACGTTTGGGGGTGCAGATGGAGGC TTCTCTGCGGGTATCCCATCACCAGATAAAGTCCATCGCAAGCGGGCCAGCTCAGAGAACGAGAGACTCC AGTACAAAACCCCACCTCCCAGCTACAATTCAGCATTGACACAGCCCGGGGTGGCCATGCCCACCTCGGG AGACTCTGAGAGAAAGGTCGCCCCTCTGTCCTCCTCCCTGGACACCTCCCTGGACTTCTCCAAAGAAAAC AAGAAAGCAGGAGTAGACCTGGGCAGCAGTGTGAGCGGAGACCACGGAAACTCGGACTCCGGCCAAGAGC AGGGGGAAGCCCTGCCTGGGCACCTGGCAGCCGTGAATGGCACTGCACTGCCCAGCGAGCAGGCTGGCCC TGCCGGCACCCTGCTGCCAGGCTCGTGCCACCCGGCCCCCTCAGCTGAGCTCTGCTGTGCAGTGGAGCAA GCAGAGGAAATCATCGGGCTGGAAGCCACAGGCTTCACCTCGGGTGACCAGCTGGAGGCGCTCAGCTGCA TCCCGGTGGACAGCGCAGTGGCAGTGGAGTGTGACGAGCAGGTGCTGGGGGAGTTTGAGGAGTTCTCTCG CAGGATCTATGCCCTCAGCGAGAACGTGTCCAGCTTCCGCCGGCCTCGCAGGAGTTCTGACAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210112 representing NM_178635
Red=Cloning site Green=Tags(s) MSSCASLGGPVPLPPPGPSAALTSGAPARALHVELPSQQRRLRHLRNIAARNIVNRNGHQLLDTYFTLHL CDNEKIFKEFYRSEVIKNSLNPTWRSLDFGIMPDRLDTSVSCFVVKIWGGKEEAFQLLIEWKVYLDGLKY LGQQIHARNQNEIIFGLNDGYYGAPCEHKGHPNAQKNLLQVDQNCVRNSYDVFSLLRLHRAQCAIKQTQV TVQRLGKEIEEKLRLTSTSNELKKESECLRLKILVLRNELERQKKALGREVAFLHKQQMALQDKGSAFST EHGKLQLQKDSLSELRKECTAKRELFLKTNAQLTIRCRQLLSELSYIYPIDLNEHKDYFVCGVKLPNSED FQAKEDGSIAVALGYTAHLVSMISFFLQVPLRYPIIHKGSRSTIKDNINDKLTEKEREFPLYPKGGEKLQ FDYGVYLLNKNIAQLRYQHGLGTPDLRQTLPNLKNFMEHGLMVRCDRHHISNAIPVPKRQSSTFGGADGG FSAGIPSPDKVHRKRASSENERLQYKTPPPSYNSALTQPGVAMPTSGDSERKVAPLSSSLDTSLDFSKEN KKAGVDLGSSVSGDHGNSDSGQEQGEALPGHLAAVNGTALPSEQAGPAGTLLPGSCHPAPSAELCCAVEQ AEEIIGLEATGFTSGDQLEALSCIPVDSAVAVECDEQVLGEFEEFSRRIYALSENVSSFRRPRRSSDK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_178635 |
ORF Size | 2094 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178635.2, NP_848750.2 |
RefSeq Size | 3432 bp |
RefSeq ORF | 2097 bp |
Locus ID | 78610 |
UniProt ID | Q8K245 |
Gene Summary | Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P). During autophagy acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3. Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2. Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events. Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion. In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204184 | Uvrag (untagged) - Mouse UV radiation resistance associated gene (Uvrag), (10ug) |
CNY 5,120.00 |
|
MR210112 | Uvrag (Myc-DDK-tagged) - Mouse UV radiation resistance associated gene (Uvrag) |
CNY 6,940.00 |
|
MR210112L3 | Lenti ORF clone of Uvrag (Myc-DDK-tagged) - Mouse UV radiation resistance associated gene (Uvrag) |
CNY 8,840.00 |
|
MR210112L4 | Lenti ORF clone of Uvrag (mGFP-tagged) - Mouse UV radiation resistance associated gene (Uvrag) |
CNY 8,840.00 |