Acsl1 (NM_007981) Mouse Tagged ORF Clone
CAT#: MG210117
- TrueORF®
Acsl1 (tGFP-tagged) - Mouse acyl-CoA synthetase long-chain family member 1 (Acsl1)
ORF Plasmid: DDK
"NM_007981" in other vectors (4)
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CNY 6,370.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Ac; Acas; Acas1; Acs; Fac; Facl2; FACS; LACS 1; LACS1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210117 representing NM_007981
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTCCATGAATTGTTCCGGTATTTTCGAATGCCAGAGCTGATTGACATTCGGCAGTACGTGCGCA CCCTTCCAACCAACACCCTCATGGGGTTTGGGGCTTTTGCAGCGCTCACCACCTTCTGGTATGCCACCAG GCCTAAGGCCCTGAAGCCACCATGTGACCTCTCCATGCAGTCAGTGGAAATAGCGGGTACCACTGATGGT ATTCGAAGATCAGCAGTCCTTGAAGATGACAAGCTCTTGGTGTACTACTACGACGATGTCAGAACCATGT ACGATGGCTTCCAGAGGGGGATTCAGGTGTCAAATAATGGTCCTTGTTTAGGTTCTCGGAAGCCAAACCA GCCCTATGAGTGGATTTCCTACAAAGAGGTGGCAGAACTGGCTGAGTGCATAGGCTCCGGGCTGATCCAG AAGGGGTTCAAGCCTTGCTCCGAGCAGTTCATCGGCCTCTTCTCTCAAAACAGACCCGAGTGGGTGATCG TCGAGCAAGGATGCTTCTCTTACTCAATGGTGGTCGTCCCGCTCTATGACACCCTTGGAGCTGACGCCAT CACCTACATAGTGAACAAAGCTGAACTCTCTGTGATTTTTGCTGACAAGCCAGAAAAAGCCAAACTCTTA TTAGAAGGTGTAGAAAACAAGTTAACACCATGCCTTAAAATCATAGTCATCATGGACTCCTACGGCAGTG ATCTGGTGGAACGAGGCAAGAAGTGTGGGGTGGAAATCATCAGCCTCAAAGCTCTGGAGGACCTTGGAAG AGTGAACAGAGTGAAGCCCAAGCCTCCAGAACCCGAAGATCTTGCGATAATTTGTTTCACAAGTGGAACT ACAGGCAACCCCAAAGGAGCAATGATCACTCACCAAAACATTATAAACGACTGCTCAGGTTTTATAAAAG CAACAGAGAGTGCATTCATCGCTTCCACAGATGATGTGCTGATATCTTTCTTGCCTCTCGCCCATATGTT TGAGACCGTTGTAGAGTGTGTAATGCTGTGTCATGGAGCTAAGATAGGATTTTTCCAAGGAGATATCAGG CTGCTTATGGACGACCTCAAGGTGCTTCAGCCCACCATCTTCCCTGTGGTTCCCAGGCTGCTGAACCGGA TGTTCGACAGAATTTTTGGACAAGCAAACACTTCCTTGAAGCGATGGCTGTTGGACTTTGCCTCCAAAAG GAAAGAGGCGGAGCTTCGCAGTGGCATCGTCAGAAACAACAGCCTGTGGGATAAACTCATCTTCCACAAG ATACAGTCGAGCCTGGGTGGGAAAGTCCGGCTGATGATCACAGGAGCAGCCCCGGTGTCTGCCACAGTGC TGACGTTTCTGAGGACAGCGCTCGGCTGCCAGTTCTATGAAGGCTACGGACAGACCGAGTGCACTGCTGG TTGCTGCCTGAGCTTGCCCGGAGACTGGACGGCAGGCCATGTTGGAGCCCCCATGCCTTGCAATTATGTA AAGCTTGTGGATGTGGAAGAAATGAATTACCTGGCATCCAAGGGCGAGGGTGAGGTGTGTGTGAAAGGGG CAAATGTGTTCAAAGGCTACTTGAAAGACCCAGCAAGAACAGCTGAAGCCCTGGATAAAGATGGCTGGTT ACACACGGGGGACATTGGAAAATGGCTGCCAAATGGCACCTTGAAGATTATCGACAGGAAAAAGCACATA TTTAAACTAGCCCAAGGAGAGTACATAGCACCAGAAAAGATTGAAAATATCTACCTGCGGAGTGAAGCCG TGGCCCAGGTGTTTGTCCACGGAGAAAGCTTGCAGGCCTTTCTCATAGCAGTTGTGGTACCCGACGTTGA GAGCCTACCGTCCTGGGCACAGAAGAGAGGCTTACAAGGGTCCTTCGAAGAACTGTGCAGGAACAAGGAT ATCAATAAAGCTATCCTGGACGACTTGTTGAAACTTGGGAAGGAAGCCGGTCTGAAGCCATTTGAACAGG TCAAAGGCATTGCTGTGCACCCGGAATTATTTTCTATTGACAACGGCCTTCTGACTCCAACACTGAAGGC GAAGAGGCCAGAGCTACGGAACTATTTCAGGTCGCAGATAGATGAACTGTACGCCACCATCAAGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210117 representing NM_007981
Red=Cloning site Green=Tags(s) MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEIAGTTDG IRRSAVLEDDKLLVYYYDDVRTMYDGFQRGIQVSNNGPCLGSRKPNQPYEWISYKEVAELAECIGSGLIQ KGFKPCSEQFIGLFSQNRPEWVIVEQGCFSYSMVVVPLYDTLGADAITYIVNKAELSVIFADKPEKAKLL LEGVENKLTPCLKIIVIMDSYGSDLVERGKKCGVEIISLKALEDLGRVNRVKPKPPEPEDLAIICFTSGT TGNPKGAMITHQNIINDCSGFIKATESAFIASTDDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIR LLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSLKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHK IQSSLGGKVRLMITGAAPVSATVLTFLRTALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYV KLVDVEEMNYLASKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHI FKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAVVVPDVESLPSWAQKRGLQGSFEELCRNKD INKAILDDLLKLGKEAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYATIKI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007981 |
ORF Size | 2097 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007981.4, NP_032007.2 |
RefSeq Size | 3891 bp |
RefSeq ORF | 2100 bp |
Locus ID | 14081 |
UniProt ID | P41216 |
Gene Summary | The protein encoded by this gene belongs to a family of acyl coenzyme A synthetase proteins, which convert long chain fatty acids to acyl CoA products via an ATP-dependent pathway. This enzyme is enriched in heart, liver and adipose tissue, where it functions in lipid synthesis and mitochondrial and peroxisomal beta-oxidation. In addition, it is expressed in monocytes and macrophages where it appears to have a functionally distinct role in mediating inflammatory and innate immune responses. A pseudogene of this gene is found on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220560 | Acsl1 (untagged) - Mouse acyl-CoA synthetase long-chain family member 1 (Acsl1), (10ug) |
CNY 9,030.00 |
|
MR210117 | Acsl1 (Myc-DDK-tagged) - Mouse acyl-CoA synthetase long-chain family member 1 (Acsl1) |
CNY 5,120.00 |
|
MR210117L3 | Lenti ORF clone of Acsl1 (Myc-DDK-tagged) - Mouse acyl-CoA synthetase long-chain family member 1 (Acsl1) |
CNY 7,700.00 |
|
MR210117L4 | Lenti ORF clone of Acsl1 (mGFP-tagged) - Mouse acyl-CoA synthetase long-chain family member 1 (Acsl1) |
CNY 7,700.00 |