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Lca5 (NM_027448) Mouse Tagged ORF Clone
CAT＃: MG210151
- TrueORF®
Lca5 (tGFP-tagged) - Mouse RIKEN cDNA 4930431B11 gene (4930431B11Rik), transcript variant 2
ORF Plasmid: DDK
"NM_027448" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930431B11Rik; 5730406O13Rik; AV274874; ORF64 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210151 representing NM_027448
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAAAGAGCCCGAAGTCCAGATATTGAACAAGGAAAAAAAGGAGGCAAACACCCATACTCTCATT ATTCGTCTGACCTTGGGTCCTCACCACAGTCCTCTGGCCCCTCATCACCTGTCAACACGACGCCATGTGC CAGTACTAGGGAGAAGAATCCCAAAAGACACTTGTCAGACAACCAAGTGCATCACCCAACCGTTAGGAAA GTAAGTCCTAAGGCTGTACCCAGCAAAAAGGGCATCCGAGTGGGATTTCGGTCCCAAAGCCTCAACAGAG AGCCACTTCGGAAAGATCCAGATATCGTAACAAAGCGGGTTCTTTCTGCCAGACTTCTGAAAATCAACGA GCTGCAGAACGAAGTGTCAGAACTACAGGTCAAGTTAGCCCAGCTGCTCAAAGAGAACAAGGCTTTGAAA AGCCTTCAGTATCGTCAGGAGAAAGCGCTGAATAAGTTTGAAGATGCGGAGAACGAGATCTCACAACTTA TACACCGCCATAACAATGAGATTACTGCGCTCAAAGAACGCTTACGAAAATCTCAGGAGAAGGAGCGGGC GACAGAGAAAAGAGTGAAAGAGACAGAAGGTGAACTATTTAGGACGAAATTTTCCTTACAGAAACTGAAA AAGATCTCCGAGGCCAGACACCTACCGGAGCGAGACGATTTGGCCAAGAAACTGGTTTCGGCAGAGTTAA AATTAGATGACACGGAGAGAAAGATTAAGGAGCTATCGAAGAACCTGGAGCTGAGCACGAACAGTTTCCA GCGGCAGCTGCTCGCTGAAAGGAAAAGAGCATTCGAGGCTTACGATGAAAATAAAGTTCTTCAAAAGGAG CTGCAGCGGCTCCATCACAAGCTCAAGGAAAAGGAGAAAGAATTGGATATAAAAAACATCTATGCTAATC GCCTGCCCAAATCCTCTCCAAAGAAAGAGAAAGAAATCGAAAGGAAACATGTATCCTGCCAGAGTGACTT TACAGACCAGTGTACAAAAGGAGTACAGACCGCTGAAGACTTTGAGCTGGAGGACTTTCCTTTCACAGCC CAGACAGTTCTCTGCTATGAAAACAGATGGGATGAACCAGAATATCTCTCTTCGTACCTGGAGTACCAGG ATCTCAACAAACATGGATCAGAGATGCTAAGTTCAGTGTTGGGACAAGAAGGAAAGTATGATGAAGATGA GGACCCATGCTCTGCAAAGCAGGAGGCCAGGAAGCCTGAGAGTGAATGGGCAAGAGAAGAACTTGATAAG GTGAAAGGCAAGTCAGCTTTGCTAGGGAGAGCAGAGAAGCTGGCATTGGAAGCTGGGAGATTCCCAACGG AAAATTATCAAGCTCAGTCTGTCGATAAATTCGAAGATGAGGCAGAAAGACTAAAGACAGAAATGCTCCT TGCCAAACTGAACGAAATCAACAAAGAACTCCAAGATCCTCAGAACCTCGGGCGGGCCCCTTTGCCATTG CTGCCCAACTTCGAATCCAAGCTGCACTCTCCAGACAGAAGTACCAGACCTTACTCGTTCCCCGAGTCCT TAGACCGATCGTTTAATGGACAACACTTGCAGGACTTGAGTTTTTTAACTCCCAGGGGAGAAGGCGGGAG TCCAGGACCGATCCGAAGTCCAGGACAGATCCGAAGTCCAGCCCCTCTAGATGAGTTTTCATTTGGTAGC TATGTGCCTTCGTTTGGAAAAACATTAGGGAAGTCAAATCCACCTAGCCAAAAAAGTAGCCTTTTAGATT TCCAAAGCAACAGTTCTGAAAGCCCGAGTAAAGACAGTCTTGATTTAATGTCGAGGAAAGAGAAAAAGGC GACTCTGATGGAGCAGCTGTTTGGCCCCAGCGCCAGCAACACCAGTGTCTCCTCCAAGAGCACCGACCCA CACTTCCCTGCTGCCAGCAGAGGTGACATGGACCCTCTTCATTTCCTCTCTGGGGACAGAAACAGCAGAG TCCGGGAGCCTGGCGATGAAGAGGAAGACCTTTTCCTCAGGGAAGGAAGAAGTTTTAATCCAAATAGACA CCGGTTGAAGCACGCAAGCAATAAGCCAACAGTCACGGCCGTTGACTCTGTTGATGAGGACATTGAAGAG GTTACCCTCAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210151 representing NM_027448
Red=Cloning site Green=Tags(s) MGERARSPDIEQGKKGGKHPYSHYSSDLGSSPQSSGPSSPVNTTPCASTREKNPKRHLSDNQVHHPTVRK VSPKAVPSKKGIRVGFRSQSLNREPLRKDPDIVTKRVLSARLLKINELQNEVSELQVKLAQLLKENKALK SLQYRQEKALNKFEDAENEISQLIHRHNNEITALKERLRKSQEKERATEKRVKETEGELFRTKFSLQKLK KISEARHLPERDDLAKKLVSAELKLDDTERKIKELSKNLELSTNSFQRQLLAERKRAFEAYDENKVLQKE LQRLHHKLKEKEKELDIKNIYANRLPKSSPKKEKEIERKHVSCQSDFTDQCTKGVQTAEDFELEDFPFTA QTVLCYENRWDEPEYLSSYLEYQDLNKHGSEMLSSVLGQEGKYDEDEDPCSAKQEARKPESEWAREELDK VKGKSALLGRAEKLALEAGRFPTENYQAQSVDKFEDEAERLKTEMLLAKLNEINKELQDPQNLGRAPLPL LPNFESKLHSPDRSTRPYSFPESLDRSFNGQHLQDLSFLTPRGEGGSPGPIRSPGQIRSPAPLDEFSFGS YVPSFGKTLGKSNPPSQKSSLLDFQSNSSESPSKDSLDLMSRKEKKATLMEQLFGPSASNTSVSSKSTDP HFPAASRGDMDPLHFLSGDRNSRVREPGDEEEDLFLREGRSFNPNRHRLKHASNKPTVTAVDSVDEDIEE VTLR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027448 |
| ORF Size | 2112 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027448.1, NP_081724.1 |
| RefSeq Size | 4235 bp |
| RefSeq ORF | 2115 bp |
| Locus ID | 75782 |
| UniProt ID | Q80ST9 |
| Gene Summary | Involved in intraflagellar protein (IFT) transport in photoreceptor cilia.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220846 | Lca5 (untagged) - Mouse Leber congenital amaurosis 5 (human) (Lca5), transcript variant 2, (10ug) |
CNY 9,120.00 |
|
| MR210151 | Lca5 (Myc-DDK-tagged) - Mouse Leber congenital amaurosis 5 (human) (Lca5), transcript variant 2 |
CNY 5,168.00 |
|
| MR210151L3 | Lenti ORF clone of Lca5 (Myc-DDK-tagged) - Mouse Leber congenital amaurosis 5 (human) (Lca5), transcript variant 2 |
CNY 7,700.00 |
|
| MR210151L4 | Lenti ORF clone of Lca5 (mGFP-tagged) - Mouse Leber congenital amaurosis 5 (human) (Lca5), transcript variant 2 |
CNY 7,700.00 |
