Stt3a (BC037612) Mouse Tagged ORF Clone

CAT#: MG210156

  • TrueORF®

Stt3a (tGFP-tagged) - Mouse STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) (cDNA clone MGC:46919



  "BC037612" in other vectors (1)

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Mouse Protein / Lysate Request

CNY 6,940.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AA408947; BB081708; Itm1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210156 representing BC037612
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTAAGCTTGGATTTTTGCGATTGTCCTATGAGAAGCAGGACACACTTCTAAAGCTTCTCATCCTGT
CGATGGCTGCTGTGTTATCTTTTTCTACTCGTCTTTTTGCTGTGCTGAGATTTGAAAGTGTCATCCATGA
GTTTGATCCGTACTTTAATTATCGGACTACCCGGTTTCTGGCTGAGGAGGGGTTTTATAAATTCCATAAC
TGGTTTGATGACCGGGCTTGGTACCCTTTGGGCCGAATCATTGGAGGAACAATTTACCCAGGTTTAATGA
TCACTTCTGCTGCAATCTACCATGTACTCCATTTCTTCCATATCACTATTGACATTCGGAATGTCTGTGT
TTTCCTGGCCCCACTTTTCTCCTCTTTCACCACCATCGTTACGTACCACCTTACCAAAGAGCTCAAGGAT
GCAGGAGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTCCTGGGTATATTTCTCGATCTGTAGCTG
GCTCCTATGATAATGAAGGAATTGCTATCTTTTGCATGCTGCTTACTTACTACATGTGGATCAAGGCAGT
GAAGACTGGTTCCATCTATTGGGCTGCCAAGTGTGCCCTCGCTTATTTCTACATGGTCTCTTCATGGGGA
GGCTATGTGTTCCTGATCAACTTGATTCCTCTACATGTCCTGGTGCTAATGCTGACAGGCCGTTTTTCTC
ACCGGATCTACGTAGCCTACTGTACTGTTTACTGCCTGGGCACCATTCTTTCTATGCAGATTTCCTTTGT
TGGTTTCCAGCCCGTCCTTTCATCAGAACACATGGCAGCCTTTGGAGTGTTTGGTCTCTGTCAGATCCAT
GCTTTCGTAGATTACCTGCGCAGCAAGTTGAATCCACAGCAATTCGAAGTTCTTTTCCGGAGTGTTATCT
CCCTGGTTGGCTTTGTCCTCCTCACTGTGGGAGCTCTCCTCATGCTAACAGGAAAAATTTCTCCCTGGAC
AGGGCGTTTCTACTCTCTGCTGGATCCCTCTTATGCTAAGAATAACATTCCCATTATTGCATCTGTTTCT
GAGCACCAGCCCACAACCTGGTCTTCCTACTATTTTGATCTACAGCTCCTTGTCTTCATGTTTCCAGTTG
GCCTCTATTACTGCTTTAGCAACCTGTCTGATGCTCGGATTTTTATCATCATGTATGGTGTGACCAGCAT
GTACTTTTCAGCTGTAATGGTGCGTCTAATGCTGGTATTGGCACCTGTTATGTGCATTCTTTCTGGCATT
GGTGTTTCCCAGGTGCTGTCCACATATATGAAAAATCTGGACATAAGTCGCCCAGACAAGAAGAGCAAGA
AGCAACAGGATTCTACTTACCCTATTAAGAATGAGGTGGCGAGTGGGATGATACTGGTCATGGCTTTTTT
TCTCATCACCTACACGTTTCATTCGACTTGGGTGACCAGTGAAGCCTATTCTTCTCCCTCCATTGTACTG
TCTGCTCGTGGTGGGGATGGCAGTAGGATCATTTTTGATGACTTCCGAGAAGCGTATTATTGGCTCCGTC
ACAATACTCCAGAGGATGCAAAAGTCATGTCATGGTGGGATTATGGCTACCAAATTACTGCAATGGCAAA
TCGGACAATTTTAGTGGACAATAACACATGGAATAATACCCATATTTCTCGAGTAGGGCAGGCAATGGCA
TCCACAGAAGAAAAAGCCTATGAAATCATGAGGGAGCTTGATGTCAGCTATGTGCTTGTCATTTTTGGAG
GCCTTACTGGGTATTCTTCGGATGATATCAACAAGTTTCTTTGGATGGTCCGGATTGGAGGAAGCACAGA
GACAGGAAGACACATTAAGGAGAATGACTACTATACTCCTACTGGGGAATTCCGTGTTGATCGTGAGGGT
TCTCCGGTGCTGCTCAACTGCCTTATGTACAAAATGTGTTACTACCGCTTTGGGCAGGTCTACACAGAAG
CCAAGCGTCCACCAGGCTTTGACCGTGTTCGAAATGCTGAGATTGGTAATAAAGACTTTGAGCTTGATGT
CCTGGAGGAAGCGTATACCACAGAACACTGGCTAGTCAGGATATACAAGGTAAAGGACCTGGATAATCGA
GGCTTGTCAAGGACA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210156 representing BC037612
Red=Cloning site Green=Tags(s)

MTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESVIHEFDPYFNYRTTRFLAEEGFYKFHN
WFDDRAWYPLGRIIGGTIYPGLMITSAAIYHVLHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKD
AGAGLLAAAMIAVVPGYISRSVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSIYWAAKCALAYFYMVSSWG
GYVFLINLIPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGLCQIH
AFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLLDPSYAKNNIPIIASVS
EHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMYGVTSMYFSAVMVRLMLVLAPVMCILSGI
GVSQVLSTYMKNLDISRPDKKSKKQQDSTYPIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVL
SARGGDGSRIIFDDFREAYYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMA
STEEKAYEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTETGRHIKENDYYTPTGEFRVDREG
SPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNR
GLSRT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC037612
ORF Size 2115 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC037612, AAH37612
RefSeq Size 2736 bp
RefSeq ORF 2117 bp
Locus ID 16430
Gene Summary Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets (By similarity). STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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