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Mre11a (NM_018736) Mouse Tagged ORF Clone
CAT＃: MG210160
- TrueORF®
Mre11a (tGFP-tagged) - Mouse meiotic recombination 11 homolog A (S. cerevisiae) (Mre11a)
ORF Plasmid: DDK
"NM_018736" in other vectors (3)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Mre11; Mre11b |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210160 representing NM_018736
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCCACAGATCCACTTGACGATGAAGACACATTTAAAATCCTGGTTGCCACTGATATTCATCTTG GATTTATGGAGAAAGATGCAGTTAGAGGAAATGATACATTTGTGACATTTGATGAAATCTTAAGACTTGC CCTGGAAAATGAAGTGGATTTTATTTTGTTAGGTGGTGATCTTTTCCATGAAAACAAGCCCTCGAGGAAA ACCCTGCACAGCTGCTTGGAGCTGCTTAGGAAGTACTGTATGGGTGACCGCCCTGTGCAGTTTGAGGTCA TCAGTGATCAGTCAGTCAACTTTGGTTTTAGTAAGTTTCCATGGGTGAACTACCAGGATGGCAATCTCAA CATTTCCATTCCAGTATTTAGTATCCACGGCAACCATGATGATCCCACGGGGGCAGATGCCCTCTGTGCC CTGGATGTTTTAAGCTGTGCTGGGTTTGTGAATCACTTTGGACGGTCAATGTCGGTGGAGAAGGTTGACA TTAGTCCGGTTCTGCTGCAGAAAGGAAGCACAAAACTCGCTCTGTACGGCTTAGGCTCCATTCCAGATGA AAGGCTCTATCGGATGTTTGTGAATAAAAAAGTAACAATGTTGAGACCAAAGGAAGATGAGAACTCATGG TTTAACTTATTTGTGATTCATCAGAACAGGAGTAAGCATGGAAACACCAACTTCATTCCAGAGCAGTTTT TGGATGACTTCATCGACCTCGTTATCTGGGGCCATGAACATGAGTGTAAAATTGGCCCAATCAAAAATGA GCAGCAGCTCTTCTATGTGTCTCAGCCCGGAAGCTCAGTGGTGACGTCCCTTTCCCCTGGAGAAGCTGTG AAGAAACATGTAGGCTTGCTGCGCATTAAAGGGAGAAAGATGAACATGCAGAAGCTGCCTCTCCGCACCG TGCGGCGGTTCTTCATAGAAGACGTGGTTCTGGCTAACCACCCAAACCTGTTCAACCCTGACAATCCTAA GGTGACCCAGGCCATCCAGAGCTTCTGTCTGGAGAAGATTGAAGAAATGCTTGACAGTGCTGAGCGGGAA CGACTGGGGAATCCTCAGCAGCCGGGGAAGCCTCTTATCCGACTACGGGTGGACTATAGTGGAGGCTTTG AACCTTTCAACGTTCTTCGTTTTAGCCAGAAGTTTGTGGATCGAGTCGCTAACCCAAAAGATGTCATCCA CTTTTTCAGGCACAGGGAACAAAAGGGAAAAACAGGAGAAGAGATCAACTTTGGGATGCTCATCACAAAA CCTGCTTCAGAAGGAGCAACGCTTAGAGTAGAAGACCTCGTAAAGCAGTATTTCCAAACTGCAGAGAAGA ATGTTCAGCTCTCGCTGCTGACAGAAAGAGGGATGGGCGAAGCAGTTCAAGAGTTTGTGGACAAGGAAGA GAAAGATGCCATCGAGGAATTAGTGAAGTACCAGCTGGAGAAAACACAGCGGTTTCTTAAGGAGCGCCAT ATTGATGCTCTGGAAGACAAGATTGATGAGGAGGTCCGACGTTTCCGAGAAAGCAGACAGAGAAATACCA ACGAAGAAGATGATGAAGTTCGAGAGGCCATGAGCAGAGCCCGGGCCCTCAGATCACAGTCAGAGACCTC CACCTCAGCCTTTAGTGCTGAGGACCTGAGCTTTGATACATCGGAGCAGACAGCAAATGACTCTGATGAC AGCCTGTCAGCAGTGCCGAGCAGAGGCCGAGGCCGAGGCCGAGGGCGAAGAGGAGCCAGAGGGCAGAGCT CGGCACCTAGAGGAGGCTCTCAGAGAGGCCGAGACACTGGGCTGGAGATCACTACTCGAGGCAGGAGCTC AAAGGCCACCTCATCAACATCTAGAAACATGTCCATTATAGACGCTTTCAGATCTACCCGACAACAGCCT TCTAGAAATGTAGCCCCTAAGAATTACTCAGAGACCATTGAGGTGGATGACTCTGACGAAGATGACATTT TTCCTACCAATTCCAGGGCTGATCAAAGGTGGTCGGGCACAACATCTAGCAAACGGATGTCCCAGAGCCA GACAGCCAAAGGGGTAGACTTTGAATCAGATGAGGATGATGACGATGACCCTTTCATGAGCAGTAGTTGC CCAAGAAGAAACCGAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210160 representing NM_018736
Red=Cloning site Green=Tags(s) MSPTDPLDDEDTFKILVATDIHLGFMEKDAVRGNDTFVTFDEILRLALENEVDFILLGGDLFHENKPSRK TLHSCLELLRKYCMGDRPVQFEVISDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCA LDVLSCAGFVNHFGRSMSVEKVDISPVLLQKGSTKLALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSW FNLFVIHQNRSKHGNTNFIPEQFLDDFIDLVIWGHEHECKIGPIKNEQQLFYVSQPGSSVVTSLSPGEAV KKHVGLLRIKGRKMNMQKLPLRTVRRFFIEDVVLANHPNLFNPDNPKVTQAIQSFCLEKIEEMLDSAERE RLGNPQQPGKPLIRLRVDYSGGFEPFNVLRFSQKFVDRVANPKDVIHFFRHREQKGKTGEEINFGMLITK PASEGATLRVEDLVKQYFQTAEKNVQLSLLTERGMGEAVQEFVDKEEKDAIEELVKYQLEKTQRFLKERH IDALEDKIDEEVRRFRESRQRNTNEEDDEVREAMSRARALRSQSETSTSAFSAEDLSFDTSEQTANDSDD SLSAVPSRGRGRGRGRRGARGQSSAPRGGSQRGRDTGLEITTRGRSSKATSSTSRNMSIIDAFRSTRQQP SRNVAPKNYSETIEVDDSDEDDIFPTNSRADQRWSGTTSSKRMSQSQTAKGVDFESDEDDDDDPFMSSSC PRRNRR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018736 |
| ORF Size | 2118 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018736.3 |
| RefSeq Size | 3034 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 17535 |
| UniProt ID | Q61216 |
| Gene Summary | Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210160 | Mre11a (Myc-DDK-tagged) - Mouse meiotic recombination 11 homolog A (S. cerevisiae) (Mre11a) |
CNY 5,168.00 |
|
| MR210160L3 | Lenti ORF clone of Mre11a (Myc-DDK-tagged) - Mouse meiotic recombination 11 homolog A (S. cerevisiae) (Mre11a) |
CNY 7,700.00 |
|
| MR210160L4 | Lenti ORF clone of Mre11a (mGFP-tagged) - Mouse meiotic recombination 11 homolog A (S. cerevisiae) (Mre11a) |
CNY 7,700.00 |
