C1r (BC093528) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	C1rb
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210169 representing BC093528 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTCTTTGCCCTCCTGGTGACCCTGTTCTATGGGGTGGAAGGCTCCATTTACCTCCCTCAGAAGC TCTATGGAGAGGTGACCTCCCCTCTGTATCCCAAGCCTTACCCCAGTGACTTGGAGACAACCACTGTGAT CACTGTCCCCATGGGGTACAGGGTGAAGCTGGTCTTCTGGCAGTTTGACGTGGAGCCTTCTGAAGGCTGC TTCTATGACTATGTTAAGATTTCTGCTGATAAGCAAACACTGGGGAGGTTCTGTGGGCAGCTGGATTCTC CCCTGGGCAACCCCCCAGGAAGCAAGGAATTCATGTCCCAAGGAAACAAGATGCTGCTGACCTTCCACAC AGACTTCTCCAATGAGGAGAATGGGACCATCATGTTCTACAAGGGCTTCCTGGCCTACTACCAGGCTGTA GACCTTGATGAATGTGCATCGCAGCCCAACTCAGTGGAAGAGGGTTTGCAGCCCCGATGCCAACATCTGT GTCACAACTATGTTGGAGGCTACTTCTGTTCCTGCCATCCTGGCTATGAGCTTCAGAAAGATGGGCAATC CTGCCAGGCTGAGTGCAGCAGTGAGCTCTACACAGAGCCCTCAGGCTATGTCTCCAGCCTTGAATACCCT CAGCCCTATCCACCGGATCTACGCTGCAACTACAGCATCCGGGTGGAGAGGGGCCTCACTGTGCACCTCA AGTTCCTGGATCCCTTTGAAATTGATGACCACCAGCAAGTGCACTGCCCCTATGACCAGCTCCAGATCTA CGCTAATGGGAAGAACTTGGGTGAATTCTGTGGAAAGCAAAGGCCTCCAGACCTTGACACCAGCAGCAAT GCAGTGGATCTGCTGTTCTTCACAGATGAGTCAGGGGACAGCCGAGGCTGGAAGCTGCACTACACCACTG AAACCATCAAGTGCCCCCAGCCCAAGGCTCTGGATGAGTTCACCATCATCCAGGATCCGCAGCCTCAGTA CCAGTTCCGGGATTACTTCATTGTCACCTGCAAGCAAGGCTACCAGCTCATGGAGGGAAATCAGGCGCTA CTCTCCTTCACAGCTGTTTGCCAGCATGATGGCACATGGCATCGTGCCATGCCCAGGTGCAAGATCAAGA ACTGTGGGCAGCCCCAAAGCCTGTCTAATGGGGACTTCCGCTACATCACCACAAAAGGCGTGACCACCTA TGAAGCCAGTATCCAGTATCACTGCCATGAACCATATTACAAGATGCTGACCAGAGCTGGCAGCAGCGAG TCCATGCGAGGGATATATACCTGCACAGCCCAAGGCATTTGGAAGAATGAAGAGGAAGGAGAGAAAATGC CCCGGTGTCTGCCAGTGTGTGGGAAACCTGTCAACCCTGTGACACAGAAGGAGCGCATCATCAGAGGGCA GCCAGCCAGGCCCGGCAACTTCCCCTGGCAGGCCTTCACCACTACCCACGGGCGAGGGGGTGGGGCCCTG CTTGGAGACCGCTGGATCCTCACAGCAGCCCACACCATCTACCCCAAGCATCACAACAAGGAAAACGACA ATGCCAACCCCAAAATGCTTGTTTTCCTGGGCCACACAAATGTGGAACAGATCAAAAAACTGGGACATCA CCCAGTCCGTAGGGTCATCATACACCCAGACTACCGCCAAGATGAACCTAACAATTTTGAAGGAGACATT GCTCTACTGGAGCTGGAAAACAGTGTCACACTGGGCCCCGAACTCCTCCCCATCTGTCTCCCAGACAATG AGACCTTCTATGGCCAAGGCCTCATGGGTTATGTCAGCGGATTCGGGATAACAGAAGATAAGTTAGCTTT CGATCTCAGGTTCGTCAGACTGCCTGTAGCTGACAGTGAGGCATGCCAGAGATGGCTCCAGACAAAAAAG GATACTTCTCCATTTTCTCAAAATATGTTCTGTTCTGGGGACCCAGCTGTACAGCAAGACGCCTGCCAAG GGGACAGTGGGGGTGTTTTTGCAGTCAGGGACAGAAATCGTGATATCTGGGTGGCTACGGGCATCGTATC CTGGGGCATTGGGTGTGGTGAGGGATATGGCTTCTACACCAAGGTACTGAATTATGTTGACTGGATCAAG AAAGAGATGGGAGACGAAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210169 representing BC093528 Red=Cloning site Green=Tags(s) MWLFALLVTLFYGVEGSIYLPQKLYGEVTSPLYPKPYPSDLETTTVITVPMGYRVKLVFWQFDVEPSEGC FYDYVKISADKQTLGRFCGQLDSPLGNPPGSKEFMSQGNKMLLTFHTDFSNEENGTIMFYKGFLAYYQAV DLDECASQPNSVEEGLQPRCQHLCHNYVGGYFCSCHPGYELQKDGQSCQAECSSELYTEPSGYVSSLEYP QPYPPDLRCNYSIRVERGLTVHLKFLDPFEIDDHQQVHCPYDQLQIYANGKNLGEFCGKQRPPDLDTSSN AVDLLFFTDESGDSRGWKLHYTTETIKCPQPKALDEFTIIQDPQPQYQFRDYFIVTCKQGYQLMEGNQAL LSFTAVCQHDGTWHRAMPRCKIKNCGQPQSLSNGDFRYITTKGVTTYEASIQYHCHEPYYKMLTRAGSSE SMRGIYTCTAQGIWKNEEEGEKMPRCLPVCGKPVNPVTQKERIIRGQPARPGNFPWQAFTTTHGRGGGAL LGDRWILTAAHTIYPKHHNKENDNANPKMLVFLGHTNVEQIKKLGHHPVRRVIIHPDYRQDEPNNFEGDI ALLELENSVTLGPELLPICLPDNETFYGQGLMGYVSGFGITEDKLAFDLRFVRLPVADSEACQRWLQTKK DTSPFSQNMFCSGDPAVQQDACQGDSGGVFAVRDRNRDIWVATGIVSWGIGCGEGYGFYTKVLNYVDWIK KEMGDEN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC093528
ORF Size	2123 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC093528, AAH93528
RefSeq Size	2707 bp
RefSeq ORF	2123 bp
Locus ID	50909
Gene Summary	C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.