Arhgap28 (BC066788) Mouse Tagged ORF Clone

CAT#: MG210171

  • TrueORF®

Arhgap28 (tGFP-tagged) - Mouse Rho GTPase activating protein 28 (cDNA clone MGC:76428 IMAGE:30049271)

ORF Plasmid: DDK tGFP



  "BC066788" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,700.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AU044757; AW550892; E130310N06
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210171 representing BC066788
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGAGGACTCGGGCGGCGTGGTGCTGACCGCCTACCACTCGCACGCGCGCTCGCAACCGCAGG
GCGCTGAGCCACGCTGCGCGTCCCGGGCCAGCCATCCGCTCAGCAGAAAATCCATTCCTCGCTGCCGACG
AATCAACAGGATGCTCTCCAATGAATCCCTGCATCCTCCCTCCTTTAGTCGCTCCAACTCTCAAGCCTCA
GTAGACAGCAGCGCATCCATGGAGGAGTTCCTGCGGGAAATTGAGAGTATCAAAGAGAGCAGCGTGGGGG
CGTCGCAGGAGCAGCCACCCACTGCTGCTGCTGCTGCTGCTGAAGTCAAGCCAGTGGATGAAGGAGAGCT
TGAAGCGGAGTGGCTACAAGATGTGGGATTGTCAACTCTGATCTCTGGCAACGAAGAGGAAGACGGTAAA
GCGCTCCTGTCTACATTGACTCGGACCCAAGCTGCTGCAGTGAAAAAGAGATACAACACATATACTCAGA
CTCTGAGGAAAAAGAATAAACAACCCGTCAGGGATGTCAGAGACATCTTCGGAGTCAGCGAATCTCCTCC
TAGTGATTCTTGTGAGCATGCTACTCAGTTGGACGGTACCAAGGAAGAAAAAGATCTGCCAGGAGTTACC
AAGACAAGCAGACCTCTGCCAGACGATGCTTCTCTCAGTAGTACCACCCTATCCAATGGCGCCCAGGATG
AAGAAGGTGGTTTTGTGGCCCTACAGAGTGGTTCTGTGTCAATACTTGAGGCTATCCCGGATATTGCTGT
TCGCACCAATGGATCTGCAGATGCTGAGCAGTCGGTTCAGAGCACATTGAGTGATGATGATTATCACGGG
AAGAACGTTCCAGCAGAGGCTGAAGAGCTGTCCTTTGAGGTCTCTTACTCAGAAATGGTGACAGAAATGC
CTGATAGAAATAAATGGAAGAAGTCGGACATTAAGAAAGAAGACTATGTCTTAACTAAATTTATCATTCA
GAAAACAAGATTTGGGTTGGCCGAGACGGGGGACCTGTCTGTGGAAGACATGAAGAAAATCCGCCACCTC
TCTCTGATTGAGCTGACAGCCTTCTTCGATACTTTTGGGATACAACTGAAGAGAAACAAAACCGAGCGAG
TGAGAGGCCGAGACAACGGGATTTTCGGAGTGCCACTTACAGTCCTCCTGGACAATGACCGGAAGAAGGA
CCCTGCAGTGAAAGTTCCCCTGGTGTTACAAAAATTTTTTCAGAAAGTCGAGGAATCAGGTCTGGAATCG
GAAGGAATTTTTCGACTCTCGGGATGTACTGCCAAAGTCAAGCAGTACCGCGAAGAACTGGATGCCAGGT
TCAATGCTGACAAGTTCAAGTGGGACAAGATGTGTCACAGAGAAGCTGCGGTCATGTTGAAAGCATTCTT
CAGAGAGCTGCCCACCTCTCTCTTCCCTGTTGAATATATACCCGCCTTCATCTCTCTGATGGAAAGAGGA
CCTGACATCAAAGTGCAGTTTCAAGCCTTGCACCTCATGGTCATGGCCCTGCCTGATGCCAACAGGGACA
CAGCGCAGGCTCTGATGGCATTCTTCAATAAAGTGATCGCCAACGAATCCAAGAACCGCATGAACCTGTG
GAACATTTCCACAGTGATGGCGCCCAACTTGTTCTTCAGCAGAAGCAAGCACTCTGACTGCGAGGAACTG
CTGCTGGCCAACACGGCGACCCACATCATCCGCCTGATGCTGAAGTACCAGAAGATTCTGTGGAAGGTTC
CATCTTTCTTGATCACCCAGGTCAGAAGGATGAATGAGGCCACCATGCTGTTGAAGAAGCAGCTCCCGAG
TATGAAGAAGCTGCTCAGGAGGAAGACCCTGGATCGAGAGGTTTCAATCCTCAAGACCTCAAAGGTACCA
CAAAAATCACCTTCCTCAAGAAGAATGTCTGATGTGCCAGAAGGTGTCATAAGGGTCCACGCTCCACTTC
TGTCCAAAGTGTCCATGGCCATTCAGCTCAACAGTCAGACCAAAGCCAAAGATGTCCTGGCGAAATTTCA
GTATAAGAACCCACTAGCAACCAAACATGTACTTCGGGGGTTTCTGTGTGTCCCTGTGCTTGACCGGTTT
CTAGATTGCCCAGTGGGCCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210171 representing BC066788
Red=Cloning site Green=Tags(s)

MEVEDSGGVVLTAYHSHARSQPQGAEPRCASRASHPLSRKSIPRCRRINRMLSNESLHPPSFSRSNSQAS
VDSSASMEEFLREIESIKESSVGASQEQPPTAAAAAAEVKPVDEGELEAEWLQDVGLSTLISGNEEEDGK
ALLSTLTRTQAAAVKKRYNTYTQTLRKKNKQPVRDVRDIFGVSESPPSDSCEHATQLDGTKEEKDLPGVT
KTSRPLPDDASLSSTTLSNGAQDEEGGFVALQSGSVSILEAIPDIAVRTNGSADAEQSVQSTLSDDDYHG
KNVPAEAEELSFEVSYSEMVTEMPDRNKWKKSDIKKEDYVLTKFIIQKTRFGLAETGDLSVEDMKKIRHL
SLIELTAFFDTFGIQLKRNKTERVRGRDNGIFGVPLTVLLDNDRKKDPAVKVPLVLQKFFQKVEESGLES
EGIFRLSGCTAKVKQYREELDARFNADKFKWDKMCHREAAVMLKAFFRELPTSLFPVEYIPAFISLMERG
PDIKVQFQALHLMVMALPDANRDTAQALMAFFNKVIANESKNRMNLWNISTVMAPNLFFSRSKHSDCEEL
LLANTATHIIRLMLKYQKILWKVPSFLITQVRRMNEATMLLKKQLPSMKKLLRRKTLDREVSILKTSKVP
QKSPSSRRMSDVPEGVIRVHAPLLSKVSMAIQLNSQTKAKDVLAKFQYKNPLATKHVLRGFLCVPVLDRF
LDCPVGQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC066788
ORF Size 2121 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC066788.1
RefSeq Size 3710 bp
RefSeq ORF 2123 bp
Locus ID 268970
Gene Summary GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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