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Ncstn (NM_021607) Mouse Tagged ORF Clone
CAT＃: MG210183
- TrueORF®
Ncstn (tGFP-tagged) - Mouse nicastrin (Ncstn)
ORF Plasmid: DDK
"NM_021607" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9430068N19Rik; AA727311; Aph2; D1Dau13e; Kiaa0253; mKIAA0253; Nct |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210183 representing NM_021607
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACGACTAGGGGCGGCTCTGGGCCTGACCCAGGAAGTCGGGGTCTTCTTCTTCTGTCTTTTTCCG TGGTACTGGCAGGATTGTGTGGGGGAAACTCAGTGGAGAGGAAAATCTACATTCCCTTAAATAAAACAGC TCCTTGTGTCCGCCTGCTCAACGCCACTCATCAGATTGGCTGCCAGTCTTCAATTAGTGGGGATACAGGG GTTATCCACGTAGTGGAGAAAGAAGAAGACCTGAAGTGGGTGTTGACCGATGGCCCCAACCCCCCTTACA TGGTTCTGCTGGAGGGCAAGCTCTTCACCAGAGATGTAATGGAGAAGCTGAAGGGAACAACCAGTAGAAT CGCTGGTCTTGCCGTGACTCTAGCCAAGCCCAACTCAACTTCAAGCTTCTCTCCTAGTGTGCAGTGCCCA AATGATGGGTTTGGTATTTACTCCAACTCCTACGGGCCAGAGTTTGCTCACTGCAAGAAAACACTGTGGA ATGAACTGGGCAACGGCTTGGCTTATGAAGACTTTAGTTTCCCCATCTTTCTTCTTGAAGATGAGAACGA AACCAAGGTCATCAAGCAGTGCTATCAAGATCACAACCTGGGTCAGAATGGCTCTGCACCAAGCTTCCCA TTGTGTGCTATGCAGCTCTTCTCACACATGCACGCCGTCATCAGCACTGCCACCTGCATGCGGCGCAGCT TCATCCAGAGCACCTTCAGCATCAACCCAGAAATCGTCTGTGACCCCTTATCTGACTACAACGTATGGAG CATGCTTAAGCCTATAAATACATCTGTGGGACTAGAACCTGACGTCAGGGTTGTGGTTGCGGCCACACGG CTGGATAGCCGGTCCTTTTTCTGGAATGTGGCCCCAGGGGCTGAAAGTGCTGTAGCCTCCTTTGTCACTC AGCTGGCTGCAGCTGAAGCTTTGCACAAGGCACCTGATGTGACCACTCTATCCCGAAATGTGATGTTTGT CTTCTTCCAGGGGGAAACTTTTGACTACATTGGCAGCTCACGGATGGTCTATGATATGGAGAACGGCAAG TTTCCCGTGCGGCTCGAGAACATCGACTCCTTCGTGGAGCTGGGACAGGTGGCCCTAAGAACTTCACTAG ATCTCTGGATGCACACAGATCCCATGTCTCAGAAAAATGAGTCTGTGAAAAACCAGGTGGAGGATCTTCT GGCCACTCTGGAGAAGAGCGGTGCTGGTGTCCCTGAAGTTGTCCTGAGGAGACTGGCCCAGTCCCAGGCC CTTCCACCTTCATCTCTACAACGATTTCTTCGGGCTCGAAACATCTCTGGCGTGGTCCTGGCTGACCACT CTGGCTCCTTCCACAATCGGTATTACCAGAGCATTTATGACACGGCTGAGAACATTAATGTGACCTATCC TGAGTGGCAGAGCCCAGAAGAGGACCTCAACTTTGTGACAGACACTGCCAAGGCACTGGCGAATGTGGCC ACAGTGCTGGCGCGTGCACTGTATGAGCTTGCAGGAGGAACCAACTTCAGCAGCTCCATCCAGGCTGATC CCCAGACAGTTACACGTCTGCTCTATGGGTTCCTGGTTAAAGCTAACAACTCATGGTTTCAGTCGATCCT GAAACATGACCTAAGGTCCTATTTGGATGACAGGCCTCTTCAACACTACATCGCCGTCTCCAGCCCTACC AACACGACTTACGTTGTGCAGTACGCCTTGGCAAACCTGACTGGCAAGGCGACCAACCTCACCCGAGAGC AGTGCCAGGATCCAAGTAAAGTCCCAAATGAGAGCAAGGATTTATATGAATACTCGTGGGTACAAGGCCC TTGGAATTCCAACAGGACAGAGAGGCTCCCACAGTGTGTGCGCTCCACAGTGCGACTGGCCAGGGCCTTG TCCCCTGCCTTTGAACTGAGTCAGTGGAGCTCCACAGAATACTCTACGTGGGCGGAGAGCCGCTGGAAAG ACATCCAAGCTCGGATATTCCTAATTGCCAGCAAAGAGCTTGAGTTCATCACGCTGATCGTGGGCTTCAG CATCCTTATCTTCTCTCTCATCGTCACCTACTGCATCAATGCCAAAGCCGACGTCCTTTTTGTTGCTCCC CGAGAGCCAGGAGCTGTGTCTTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210183 representing NM_021607
Red=Cloning site Green=Tags(s) MATTRGGSGPDPGSRGLLLLSFSVVLAGLCGGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTG VIHVVEKEEDLKWVLTDGPNPPYMVLLEGKLFTRDVMEKLKGTTSRIAGLAVTLAKPNSTSSFSPSVQCP NDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFP LCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSVGLEPDVRVVVAATR LDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLSRNVMFVFFQGETFDYIGSSRMVYDMENGK FPVRLENIDSFVELGQVALRTSLDLWMHTDPMSQKNESVKNQVEDLLATLEKSGAGVPEVVLRRLAQSQA LPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPEWQSPEEDLNFVTDTAKALANVA TVLARALYELAGGTNFSSSIQADPQTVTRLLYGFLVKANNSWFQSILKHDLRSYLDDRPLQHYIAVSSPT NTTYVVQYALANLTGKATNLTREQCQDPSKVPNESKDLYEYSWVQGPWNSNRTERLPQCVRSTVRLARAL SPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELEFITLIVGFSILIFSLIVTYCINAKADVLFVAP REPGAVSY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021607 |
| ORF Size | 2124 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021607.2, NP_067620.2 |
| RefSeq Size | 2731 bp |
| RefSeq ORF | 2127 bp |
| Locus ID | 59287 |
| UniProt ID | P57716 |
| Gene Summary | Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203928 | Ncstn (untagged) - Mouse nicastrin (Ncstn), (10ug) |
CNY 5,192.00 |
|
| MR210183 | Ncstn (Myc-DDK-tagged) - Mouse nicastrin (Ncstn) |
CNY 5,192.00 |
|
| MR210183L3 | Lenti ORF clone of Ncstn (Myc-DDK-tagged) - Mouse nicastrin (Ncstn) |
CNY 7,700.00 |
|
| MR210183L4 | Lenti ORF clone of Ncstn (mGFP-tagged) - Mouse nicastrin (Ncstn) |
CNY 7,700.00 |
