Irak1 (BC004778) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	IRAK, mPLK
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210252 representing BC004778 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCCCTGGAGCCCGCCGACTGGTGCCAGTTCGCGGCCTTGATCGTGCGCGACCAGACAGAGCTGC GGCTGTGCGAGCGCTCCGAGCAGCGCACAGCCAGTGTTCTGTGGCCCTGGATCAACCGCAACGCGCGCGT AGCTGACCTCGTTCACATCCTCACGCACCTGCAGCTGCTGCGTGCGAGGGACATCATCACAGCCTGGCAC CCTCCTGCCCCCGTTGTGCCCCCAAGCACCGCTGCCCCAAGGCCCAGCAGCATCTCTGCAGGCTCTGAGG CCGGGGACTGGAGCCCCCGGAAATTGCAGTCCTCTGCCTCCACCTTCCTCTCCCCAGCTTTTCCAGGCTC CCAGACCCATTCTGAGTCAGAGCTCCTCCAGGTTCCACTCCCTGTTTCCCTCGGGCCACCACTACCATCT TCAGCCCCTTCCTCCACCAAGCAGTCAAGCCCAGAGAGCCCAGTGTCTGGCCTCCAAAGAGCCCATCCCT CCCCGTTTTGCTGGCCCTTCTGTGAGATTTCCCAAGGCACCTGCAACTTCTCTGAAGAACTCAGGATTGG AGAGGGTGGTTTTGGATGTGTGTACCGAGCAGTCATGAGAAATACTACATATGCTGTGAAGAGACTGAAG GAGGAAGCTGACCTAGAGTGGACTATGGTGAAACAGAGCTTCTTAACAGAGGTGGAACAGCTATCAAGGT TTCGTCACCCAAATATCGTAGACTTTGCTGGCTACTGTGCAGAGAGTGGCTTATACTGCCTTGTTTATGG CTTCTTGCCCAATGGCTCCTTGGAGGATCAGCTCCACCTTCAGACCCAGGCCTGCTCCCCACTTTCCTGG CCTCAACGACTGGACATTCTTCTGGGCACAGCCCGGGCTATTCAGTTTTTACATCAGGATAGCCCCAGCC TTATCCATGGAGACATCAAGAGTTCTAACGTGCTTCTGGATGAGAGACTGATGCCCAAGCTGGGAGACTT TGGCCTGGCTCGTTTCAGCCGCTTTGCGGGGGCCAAAGCAAGCCAGAGCAGTACTGTGGCCCGGACTTCC ACAGTTCGAGGTACCCTCGCCTACTTGCCTGAGGAGTACATCAAGACAGGCCGACTGGCTGTGGACACCG ATACCTTCAGCTTTGGGGTGGTAATACTGGAGACCCTTGCTGGTCAGAGGGCTGTGAGGACACAAGGTGC AAAGACCAAGTATTTGAAAGACCTGATTGAAGATGAGGCTGAAGAGGCTGGAGTGACCCTGAAAAGCACC CAGCCTACTCTGTGGGTGGGTGTAGCCACGGATGCTTGGGCTGCTCCAATTGCTGCCCAGATCTATAAGA AGCACCTGGACTCCAGACCTGGGCCCTGCCCACCCCAGTTGGGCCTGGCCCTGGCTCAACTAGCTTGCTG CTGCATGCACCGTCGGGCCAAGAAGAGGCCCCCCATGACCCAGAGGCAAAACTCCCAACATTCCTTGCTT GCCCTTTGGCTCTATTTGGGGAGGTGCTCTGTGGAACTAAGTGGTAAAGGGCTGCTGCTTTGCTGGATGA GCCTCAGATCCCAGGAACAGGTATACAAGAGACTAGAAGGGCTTCAGGCAGGGCCTCCCTGGGAGCTAGA GGTTGCCGGCCATGGCTCCCCTTCCCCACAGGAGAACTCCTACATGTCTACCACTGGCAGTGCCCAGAGT GGGGATGAACCATGGCAGCCTCTAGTAGTGACCACAAGAGCCCCAGCCCAGGCTGCCCAGCAACTCCAGA GAAGTCCCAACCAGCCAGTGGAAAGTGATGAGAGTGTTCCCGGCCTCTCTGCTACCCTGCATTCCTGGCA CTTGACTCCAGGTTCCCACCCAAGCCCAGCGTCCTTCAGAGAGGCTAGCTGTACCCAGGGAGGCACTACC AGAGAATCAAGTGTGAGGAGTAGCCCAGGCTTCCAGCCTACAACCATGGAAGGCTCACCCACGGGCAGCT CATCCCTGCTGTCATCAGAACCACCACAGATCATCATCAACCCAGCCCGACAGAAGATGGTACAAAAGCT GGCTCTTTATGAAGAAGGGGTCTTGGATAGCCTGCAACTGCTGTCATCAGGCTTTTTCCCAGGCTTGGAT TTAGAACCTGAAAAGAGCCAGGGACCTGAAGAAAGTGATGAATTCCAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210252 representing BC004778 Red=Cloning site Green=Tags(s) MDALEPADWCQFAALIVRDQTELRLCERSEQRTASVLWPWINRNARVADLVHILTHLQLLRARDIITAWH PPAPVVPPSTAAPRPSSISAGSEAGDWSPRKLQSSASTFLSPAFPGSQTHSESELLQVPLPVSLGPPLPS SAPSSTKQSSPESPVSGLQRAHPSPFCWPFCEISQGTCNFSEELRIGEGGFGCVYRAVMRNTTYAVKRLK EEADLEWTMVKQSFLTEVEQLSRFRHPNIVDFAGYCAESGLYCLVYGFLPNGSLEDQLHLQTQACSPLSW PQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLMPKLGDFGLARFSRFAGAKASQSSTVARTS TVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVILETLAGQRAVRTQGAKTKYLKDLIEDEAEEAGVTLKST QPTLWVGVATDAWAAPIAAQIYKKHLDSRPGPCPPQLGLALAQLACCCMHRRAKKRPPMTQRQNSQHSLL ALWLYLGRCSVELSGKGLLLCWMSLRSQEQVYKRLEGLQAGPPWELEVAGHGSPSPQENSYMSTTGSAQS GDEPWQPLVVTTRAPAQAAQQLQRSPNQPVESDESVPGLSATLHSWHLTPGSHPSPASFREASCTQGGTT RESSVRSSPGFQPTTMEGSPTGSSSLLSSEPPQIIINPARQKMVQKLALYEEGVLDSLQLLSSGFFPGLD LEPEKSQGPEESDEFQS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC004778
ORF Size	2151 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004778, AAH04778
RefSeq Size	2893 bp
RefSeq ORF	2153 bp
Locus ID	16179
Gene Summary	Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3 (By similarity).[UniProtKB/Swiss-Prot Function]

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