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Plod1 (NM_011122) Mouse Tagged ORF Clone
CAT＃: MG210322
- TrueORF®
Plod1 (tGFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (Plod1)
ORF Plasmid: DDK
"NM_011122" in other vectors (3)
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CNY 8,080.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2410042F05Rik; AI854890; AV007929; Lh1; Plod |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210322 representing NM_011122
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGTCCCTGCTGCTCCTGGCCCCGTTGGCCTGGCTTCTTCTGGTCCAGGCGAAGGATGACGCCAAGC TAGAGGACAACCTTCTGGTCCTCACTGTGGCCACAAAAGAGACTGAGGGCTTCCGCCGCTTCAAGCGTTC AGCTCAGTTCTTCAACTACAAGATCCAGTCACTGGGCCTGGGGGAGGACTGGAGTGTGGATGGAGGACCA GCAGCAGCAGGTGGAGGACAGAAGGTTCGGCTGCTGAAGAAGGCACTGGAAAAGCACGCAGACAAGGAAG ACCTGGTTATTCTCTTCGTTGACAGTTATGACGTGGTGTTTGCCTCGGGACCCCGGGAACTCTTAAAGAA GTTCCAGCAGGCCAAGAGCCAGGTGGTCTTCTCCGCAGAGGAGCATATCTACCCGGACCGGAGGCTGGAG GCCAAGTATCCCACAGTCCCCGACGGCAAGCGGTTCCTGGGCTCTGGAGGCTTCATTGGTTATGCCCCCA GCCTCAGCAAACTGGTGGCCGAGTGGGAAGGCCAGGACAGTGACAGCGACCAGCTCTTTTATACCAAGAT CTTCTTGAACCCGGAGAAGAGGGAGCAAATCAATATCAGCCTGGACCATCGCTGCCGAATCTTCCAGAAC CTGGATGGAGCCTTGGATGAAGTTGTGCTCAAGTTTGAAATGGGCCACGTGAGAGCACGAAATCTGGCCT ATGACACCCTCCCAGTGGTCGTTCATGGCAATGGGCCCACCAAGCTGCAGCTGAACTACCTGGGCAACTA CATTCCTCGATTCTGGACCTTCGAGACGGGCTGCACCGTGTGTGACGAGGGCCTACGGAGCCTCAAGGGC ATTGGGGATGAAGCTCTCCCCACTGTCCTGGTCGGTGTGTTCATTGAGCAGCCCACACCATTTCTATCCT TGTTTTTCCTGCGGCTGCTGCGCCTACGATACCCTCAGAAACAGATGCGGCTCTTCATTCACAACCAGGA ACGGCACCACAAACTTCAGGTAGAGCAGTTCCTGGCGGAGCACGGCAGTGAGTACCAGTCTGTGAAGCTA GTGGGTCCTGAGGTGCGCATGGCCAACGCCGACGCCAGGAACATGGGCGCAGACCTGTGCCGTCAGGACC AAACTTGCACCTACTACTTCAGTGTGGATGCTGATGTGGCTCTGACGGAGCCGAACAGCCTAAGGCTCCT GATTGAACAAAACAAGAATGTCATTGCGCCACTCATGACCCGCCACGGGAGGCTCTGGTCCAACTTCTGG GGGGGTCTGAGTGCAGATGGGTACTACGCCCGCTCAGAGGACTACGTGGACATTGTGCAGGGCCGTCGTG TTGGCGTCTGGAACGTGCCTTACATCTCAAACATCTACCTGATCAAGGGCAGTGCCCTGCGGGCTGAACT GCAGAACGTAGACCTTTTCCACTACAGCAAGCTGGATTCCGACATGAGTTTCTGCGCCAATGTCCGACAG CAGGAGGTGTTCATGTTCCTGACCAACCGGCACACCTTTGGCCACCTGCTTTCCCTGGATAACTACCAGA CCACCCACCTACATAATGATCTCTGGGAGGTGTTCAGCAACCCTGAGGACTGGAAAGAAAAGTACATCCA TGAGAATTACACCAAGGCCCTGGCGGGGAAGCTGGTGGAGACGCCTTGTCCGGATGTCTACTGGTTCCCC ATCTTCACGGAGGCGGCCTGTGATGAGCTGGTGGAGGAGATGGAACACTATGGCCAGTGGTCTCTGGGTG ATAATAAGGACAACCGGATCCAGGGTGGCTACGAAAACGTGCCCACTATCGACATCCATATGAACCAGAT CACCTTCGAGCGGGAGTGGCACAAGTTCCTGGTGGAGTACATCGCCCCCATGACAGAGAAGCTGTACCCT GGCTACTACACTAGGGCCCAGTTTGATCTAGCCTTCGTCGTCCGCTATAAGCCCGATGAGCAGCCTTCCT TGATGCCCCACCATGACGCCTCTACCTTCACCGTCAACATAGCCCTGAACAGGGTTGGGGAAGATTATGA GGGCGGAGGTTGCCGATTTCTGCGCTACAACTGCTCCGTGAGGGCACCAAGGAAGGGCTGGGCCCTCCTG CACCCCGGGCGGCTCACACACTATCACGAGGGGCTTCCTACTACCAAGGGCACGCGCTACATTGCTGTGT CTTTCGTCGATCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210322 representing NM_011122
Red=Cloning site Green=Tags(s) MRSLLLLAPLAWLLLVQAKDDAKLEDNLLVLTVATKETEGFRRFKRSAQFFNYKIQSLGLGEDWSVDGGP AAAGGGQKVRLLKKALEKHADKEDLVILFVDSYDVVFASGPRELLKKFQQAKSQVVFSAEEHIYPDRRLE AKYPTVPDGKRFLGSGGFIGYAPSLSKLVAEWEGQDSDSDQLFYTKIFLNPEKREQINISLDHRCRIFQN LDGALDEVVLKFEMGHVRARNLAYDTLPVVVHGNGPTKLQLNYLGNYIPRFWTFETGCTVCDEGLRSLKG IGDEALPTVLVGVFIEQPTPFLSLFFLRLLRLRYPQKQMRLFIHNQERHHKLQVEQFLAEHGSEYQSVKL VGPEVRMANADARNMGADLCRQDQTCTYYFSVDADVALTEPNSLRLLIEQNKNVIAPLMTRHGRLWSNFW GGLSADGYYARSEDYVDIVQGRRVGVWNVPYISNIYLIKGSALRAELQNVDLFHYSKLDSDMSFCANVRQ QEVFMFLTNRHTFGHLLSLDNYQTTHLHNDLWEVFSNPEDWKEKYIHENYTKALAGKLVETPCPDVYWFP IFTEAACDELVEEMEHYGQWSLGDNKDNRIQGGYENVPTIDIHMNQITFEREWHKFLVEYIAPMTEKLYP GYYTRAQFDLAFVVRYKPDEQPSLMPHHDASTFTVNIALNRVGEDYEGGGCRFLRYNCSVRAPRKGWALL HPGRLTHYHEGLPTTKGTRYIAVSFVDP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011122 |
| ORF Size | 2184 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011122.3 |
| RefSeq Size | 2872 bp |
| RefSeq ORF | 2187 bp |
| Locus ID | 18822 |
| UniProt ID | Q9R0E2 |
| Gene Summary | Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (PubMed:27119146). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (By similarity). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (PubMed:27119146).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210322 | Plod1 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (Plod1) |
CNY 7,320.00 |
|
| MR210322L3 | Lenti ORF clone of Plod1 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (Plod1) |
CNY 9,220.00 |
|
| MR210322L4 | Lenti ORF clone of Plod1 (mGFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (Plod1) |
CNY 9,220.00 |
