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Rfx3 (NM_011265) Mouse Tagged ORF Clone
CAT＃: MG210451
- TrueORF®
Rfx3 (tGFP-tagged) - Mouse regulatory factor X, 3 (influences HLA class II expression) (Rfx3)
ORF Plasmid: DDK
"NM_011265" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C230093O12Rik; MRFX3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210451 representing NM_011265
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGACTTCAGAGACGGGTTCAGACACAGGTTCGACAGTGACTCTGCAGACGTCTGTGGCTAGCCAAG CAGCAGTGCCTACACAGGTGGTACAGCAAGTGCCAGTGCAGCAGCAGGTGCAGCAGGTACAGACAGTTCA GCAGGTCCAACATGTCTACCCAGCTCAGGTGCAGTATGTGGAAGGAAGTGATACTGTCTATACCAATGGA GCAATCCGAACAACAACTTATCCCTACACAGAAACACAGATGTACAGCCAAAACACTGGAGGAAATTACT TTGATACTCAAGGAAGTTCTGCCCAGGTGACAACTGTGGTGTCCTCCCACAGTATGGTGGGTACTGGTGG GATTCAGATGGGCGTCACAGGAGGACAACTCATCAGCAGCTCGGGAGGAACCTATTTGATCGGCAATTCA ATGGAGAACTCTGGTCACTCAGTGACACACACAACTCGGGCCTCCCCAGCAACAATTGAAATGGCGATTG AGACGCTGCAAAAGTCTGACGGTCTGTCCACTCACAGAAGCTCTCTTCTCAACAGCCATCTGCAATGGCT GCTGGACAATTATGAGACAGCGGAGGGAGTGAGCCTGCCGAGGAGCACGCTGTACAACCACTACCTTCGA CACTGCCAGGAGCACAAGCTGGACCCGGTCAATGCCGCCTCTTTCGGAAAACTAATAAGGTCCATTTTTA TGGGGCTACGAACTAGGAGATTAGGCACTAGAGGAAACTCCAAATACCATTACTATGGGATTCGTGTGAA GCCAGATTCTCCTCTTAACCGTCTACAAGAAGATATGCAGTACATGGCTATGAGGCAGCAGCCCATGCAA CAGAAGCAAAGGTACAAGCCTATGCAGAAAGTGGATGGGGTTGCAGATGGTTTCACAGGAAGTGGCCAAC AGACAGGCACATCTGTTGAGCAAACTGTAATTGCCCAAAGTCAACATCATCAACAGTTTTTAGATGCATC TCGAGCACTTCCAGAGTTTGGAGAAGTTGAAATCTCTTCTCTGCCAGATGGTACTACCTTTGAGGATATC AAGTCACTGCAGAGTCTTTATCGAGAGCACTGTGAGGCAATACTGGACGTTGTTGTGAATCTTCAGTTTA GCCTGATAGAAAAACTGTGGCAAACATTCTGGCGCTATTCCCCATCTACTCCAGCCGACGGCACTACCAT CACGGAATCAAGCAATCTGAGTGAAATAGAAAGTCGACTTCCGAAAGCAAAGCTGATAACTCTGTGCAAA CATGAGTCTATCCTGAAATGGATGTGTAACTGTGACCATGGGATGTACCAGGCTTTGGTGGAGATTCTCA TCCCCGACGTCCTTAGACCCATTCCTAGTGCCTTGACCCAAGCCATTCGAAATTTCGCAAAAAGCCTAGA AGGTTGGCTTTCAAATGCCATGAACAATATTCCACAGAGAATGATTCAAACCAAGGTTGCCGCTGTAAGT GCCTTTGCTCAGACTCTGCGAAGATACACATCGCTCAATCACCTGGCCCAGGCAGCCCGCGCAGTGCTTC AGAACACTTCCCAGATCAACCAGATGCTCAGTGACCTCAACCGCGTTGACTTTGCCAATGTCCAGGAGCA GGCTTCCTGGGTGTGCCAGTGTGATGACAACATGGTTCAGAGACTAGAAACAGACTTCAAGATGACCCTG CAGCAGCAGAGCACTCTGGAGCAGTGGGCTGCTTGGCTGGACAATGTGATGATGCAAGCGCTGAAGCCCT ATGAAGGAAGGCCCAGCTTCCCGAAGGCTGCTCGGCAGTTTCTGCTCAAGTGGTCTTTCTACAGCTCCAT GGTTATCCGGGACTTAACCTTGCGCAGTGCTGCTAGCTTTGGCTCCTTTCACCTGATCCGGCTGCTCTAT GATGAGTACATGTTCTACTTAGTAGAGCATCGTGTTGCTCAAGTGACAGGAGAGACACCGATAGCAGTGA TGGGTGAGTTTGGCGATTTAAATGCTGTATCTCCCGGAAATCTGGATAAAGATGAAGGCAGCGAAGTCGA AAGTGAGACGGATGAAGATCTGGATGATTCTTCAGAGCCTCGGGCCAAAAGGGAGAAAACGGAGCTGAGC CAGGCGTTCCCAGTAGGCTGCATGCAGCCTGTTCTGGAGAGTGCTGTGCAGCCCAGCCTCCTGAACCCCC TACACAGCGAGCACATCGTCACAAGTACACAGACCATCAGGCAGTGCAGCGCCACAGGCAACACCTACAC TGCAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210451 representing NM_011265
Red=Cloning site Green=Tags(s) MQTSETGSDTGSTVTLQTSVASQAAVPTQVVQQVPVQQQVQQVQTVQQVQHVYPAQVQYVEGSDTVYTNG AIRTTTYPYTETQMYSQNTGGNYFDTQGSSAQVTTVVSSHSMVGTGGIQMGVTGGQLISSSGGTYLIGNS MENSGHSVTHTTRASPATIEMAIETLQKSDGLSTHRSSLLNSHLQWLLDNYETAEGVSLPRSTLYNHYLR HCQEHKLDPVNAASFGKLIRSIFMGLRTRRLGTRGNSKYHYYGIRVKPDSPLNRLQEDMQYMAMRQQPMQ QKQRYKPMQKVDGVADGFTGSGQQTGTSVEQTVIAQSQHHQQFLDASRALPEFGEVEISSLPDGTTFEDI KSLQSLYREHCEAILDVVVNLQFSLIEKLWQTFWRYSPSTPADGTTITESSNLSEIESRLPKAKLITLCK HESILKWMCNCDHGMYQALVEILIPDVLRPIPSALTQAIRNFAKSLEGWLSNAMNNIPQRMIQTKVAAVS AFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCDDNMVQRLETDFKMTL QQQSTLEQWAAWLDNVMMQALKPYEGRPSFPKAARQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLY DEYMFYLVEHRVAQVTGETPIAVMGEFGDLNAVSPGNLDKDEGSEVESETDEDLDDSSEPRAKREKTELS QAFPVGCMQPVLESAVQPSLLNPLHSEHIVTSTQTIRQCSATGNTYTAV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011265 |
| ORF Size | 2247 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011265.3 |
| RefSeq Size | 2754 bp |
| RefSeq ORF | 2250 bp |
| Locus ID | 19726 |
| UniProt ID | P48381 |
| Gene Summary | Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells (PubMed:15121860, PubMed:19671664). Also required for ciliated ependymal cell differentiation (PubMed:16930429). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells (PubMed:17229940). Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes (By similarity). Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5). Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203934 | Rfx3 (untagged) - Mouse regulatory factor X, 3 (influences HLA class II expression) (Rfx3), transcript variant 1, (10ug) |
CNY 5,496.00 |
|
| MR210451 | Rfx3 (Myc-DDK-tagged) - Mouse regulatory factor X, 3 (influences HLA class II expression) (Rfx3), transcript variant 1 |
CNY 5,496.00 |
|
| MR210451L3 | Lenti ORF clone of Rfx3 (Myc-DDK-tagged) - Mouse regulatory factor X, 3 (influences HLA class II expression) (Rfx3), transcript variant 1 |
CNY 7,700.00 |
|
| MR210451L4 | Lenti ORF clone of Rfx3 (mGFP-tagged) - Mouse regulatory factor X, 3 (influences HLA class II expression) (Rfx3), transcript variant 1 |
CNY 7,700.00 |
