Mapk7 (BC100398) Mouse Tagged ORF Clone

CAT#: MG210486

  • TrueORF®

Mapk7 (tGFP-tagged) - Mouse mitogen activated protein kinase 7 (cDNA clone MGC:118076 IMAGE:30470655)



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Mouse Protein / Lysate Request

CNY 6,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms BMK1, ERK5, PRKM7, Erk5-T
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210486 representing BC100398
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAACCGCTGAAGGAGGAGGACGGCGAAGATGGCTCTGGGGAGCCCCCTGGGAGGGTGAAGGCAG
AACCCGTTCACACCGCTGCCTCTGTGGTGGCCAAGAACCTGGCCCTGCTCAAAGCCCGCTCCTTCGACGT
GACCTTTGACGTGGGGGACGAGTACGAGATCATCGAGACCATAGGCAATGGGGCCTACGGGGTGGTGTCT
TCGGCGCGCCGCCGCCTCACGGGCCAGCAGGTGGCCATCAAGAAGATACCTAATGCTTTTGATGTGGTGA
CCAATGCCAAACGGACCCTCAGGGAGCTGAAGATCCTCAAACACTTCAAACACGACAATATCATCGCCAT
CAAGGACATCCTGAAGCCTACTGTGCCCTATGGAGAATTCAGATCTGTCTATGTGGTACTGGACCTCATG
GAGAGCGACCTACACCAGATCATTCACTCTTCACAGCCGCTCACCCTGGAACATGTGAGATACTTCCTGT
ACCAGCTGCTTCGGGGCCTCAAATACATGCACTCTGCTCAGGTCATCCACCGTGATCTTAAACCCTCTAA
CCTTCTGGTCAATGAGAACTGTGAGCTCAAGATCGGTGACTTTGGAATGGCCCGTGGTCTCTGTACTTCC
CCTGCCGAGCACCAGTACTTCATGACTGAGTATGTGGCTACTCGCTGGTACCGTGCCCCGGAGCTCATGC
TTTCCCTGCACGAGTATACGCAGGCAATCGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCTGGC
TCGGCGCCAGCTCTTCCCAGGCAAAAACTACGTGCACCAGTTACAGCTGATCATGATGGTGTTGGGAACT
CCGTCACCAGCTGTGATTCAGGCTGTGGGGGCTGAAAGGGTGCGAGCCTATATCCAGAGCCTGCCACCAA
GGCAACCTGTGCCTTGGGAGACAGTATACCCAGGTGCTGACCGCCAGGCCCTCTCCCTGCTGGGACGCAT
GTTGCGATTTAAACCCAGTGCCCGAATCTCAGCTGCTGCTGCCCTTCGCCACCCCTTCCTGGCTAAGTAC
CATGACCCTGATGATGAGCCTGATTGCGCCCCACCTTTTGACTTTGCTTTTGACCGTGAAGCCCTTACCA
GGGAGCGCATTAAGGAGGCCATTGTGGCTGAGATTGAGGACTTCCACGCACGACGGGAGGGCATCCGCCA
ACAAATCCGCTTCCAGCCTTCTCTGCAGCCTGTGGCTAGTGAGCCTGTGTGTCCAGATGTTGAGATGCCC
AGTCCCTGGGCTCCAAGTGGAGACTGTGCCATGGAGTCGCCTCCTCCAGCACTGCCACCATGCTCTGATC
CTGCACCTGACACCGTTGATCTGACTCTGCAGCCTGCCCCGCCGGCCAGTGAGCTTGCTCCACCAAAAAG
AGAGGGTGCCATCTCCGACAATACCAAAGCAGCCCTCAAAGCTGCCCTGCTCAAGTCCCTAAGGAGCAGG
CTCAGAGATGGGCCCAGTGCACCCTTGGAGGCGCCTGAGCCTCGAAAGCCCGTGACAGCTCAGGAACGCC
AGCGGGAACGAGAAGAGAAGCGCAGGAGGCGACAAGAGAGAGCCAAGGAGCGGGAGAAGCGACGACAAGA
GAGAGAACGCAAGGAGAGGGGGGCTGGTACCTTGGGGGGCCCCTCTACTGACCCTCTGGCTGGACTGGTG
CTCAGTGACAATGACCGAAGCCTGCTAGAGCGGTGGACTCGCATGGCTAGGCCTCCTGCCCCTGCCCCTG
CCCCAGCGCCAGCACCAGCGCCAGCACCGTCCTCTGCCCAGCCCACTAGTACTCCTACTGGCCCCGTATC
TCAGTCTACTGGTCCTCTACAGCCTGCAGGCTCTATTCCGGGTCCTGCCTCCCAGCCTGTTTGCCCACCC
CCTGGCCCTGTTCCCCAGCCTGCTGGCCCTATCCCTGCTCCGCTCCAGACTGCCCCTTCCACTAGCCTTT
TGGCCTCCCAGTCACTTGTGCCACCTAGTGGGTTGCCTGGTTCTGGTGCCCCAGAAGTTCTGCCTTACTT
CCCATCTGGCCCACCACCTCCAGATCCTGGGCTCACCCCTCAGCCTTCTACATCAGAGTCACCTGATGTC
AACCTGGTGACTCAGCAGCTGTCCAAGTCTCAGGTGGAGGACCCCCTGCCTCCTGTGTTCTCTGGCACTC
CAAAGGGCAGTGGGGCTGGCTACGGAGTTGGCTTTGATCTGGAGGAATTCTTAAATCAATCTTTTGATAT
GGGTGTGGCTGATGGGCCACAGGATGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210486 representing BC100398
Red=Cloning site Green=Tags(s)

MAEPLKEEDGEDGSGEPPGRVKAEPVHTAASVVAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVS
SARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILKPTVPYGEFRSVYVVLDLM
ESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTS
PAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGT
PSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFKPSARISAAAALRHPFLAKY
HDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPVCPDVEMP
SPWAPSGDCAMESPPPALPPCSDPAPDTVDLTLQPAPPASELAPPKREGAISDNTKAALKAALLKSLRSR
LRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGTLGGPSTDPLAGLV
LSDNDRSLLERWTRMARPPAPAPAPAPAPAPAPSSAQPTSTPTGPVSQSTGPLQPAGSIPGPASQPVCPP
PGPVPQPAGPIPAPLQTAPSTSLLASQSLVPPSGLPGSGAPEVLPYFPSGPPPPDPGLTPQPSTSESPDV
NLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC100398
ORF Size 2270 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC100398, AAI00399
RefSeq Size 3077 bp
RefSeq ORF 2270 bp
Locus ID 23939
Gene Summary Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression (By similarity). Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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