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Fap (NM_007986) Mouse Tagged ORF Clone
CAT＃: MG210517
- TrueORF®
Fap (tGFP-tagged) - Mouse fibroblast activation protein (Fap)
ORF Plasmid: DDK
"NM_007986" in other vectors (5)
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CNY 7,184.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SIMP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210517 representing NM_007986
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACATGGCTGAAAACTGTCTTTGGAGTTACCACCCTGGCTGCGCTTGCTTTAGTGGTGATATGCA TTGTCTTACGTCCCTCAAGAGTTTACAAACCTGAAGGAAACACAAAGAGAGCTCTTACCTTGAAGGATAT TTTAAATGGAACATTCTCATATAAAACATATTTTCCCAACTGGATTTCAGAACAAGAATATCTTCATCAA TCTGAGGATGATAACATAGTATTTTATAATATTGAAACAAGAGAATCATATATCATTTTGAGTAATAGCA CCATGAAAAGTGTGAATGCTACAGATTATGGTTTGTCACCTGATCGGCAATTTGTGTATCTAGAAAGTGA TTATTCAAAGCTCTGGCGATATTCATACACAGCGACATACTACATCTACGACCTTCAGAATGGGGAATTT GTAAGAGGATACGAGCTCCCTCGTCCAATTCAGTATCTATGCTGGTCGCCTGTTGGGAGTAAATTAGCAT ATGTATATCAAAACAATATTTATTTGAAACAAAGACCAGGAGATCCACCTTTTCAAATAACTTATACTGG AAGAGAAAATAGAATATTTAATGGAATACCAGACTGGGTTTATGAAGAGGAAATGCTTGCCACAAAATAT GCTCTTTGGTGGTCTCCAGATGGAAAATTTTTGGCATATGTAGAATTTAATGATTCAGATATACCAATTA TTGCCTATTCTTATTATGGTGATGGACAGTATCCTAGAACTATAAATATTCCATATCCAAAGGCTGGGGC TAAGAATCCGGTTGTTCGTGTTTTTATTGTTGACACCACCTACCCTCACCACGTGGGCCCAATGGAAGTG CCAGTTCCAGAAATGATAGCCTCAAGTGACTATTATTTCAGCTGGCTCACATGGGTGTCCAGTGAACGAG TATGCTTGCAGTGGCTAAAAAGAGTGCAGAATGTCTCAGTCCTGTCTATATGTGATTTCAGGGAAGACTG GCATGCATGGGAATGTCCAAAGAACCAGGAGCATGTAGAAGAAAGCAGAACAGGATGGGCTGGTGGATTC TTTGTTTCGACACCAGCTTTTAGCCAGGATGCCACTTCTTACTACAAAATATTTAGCGACAAGGATGGTT ACAAACATATTCACTACATCAAAGACACTGTGGAAAATGCTATTCAAATTACAAGTGGCAAGTGGGAGGC CATATATATATTCCGCGTAACACAGGATTCACTGTTTTATTCTAGCAATGAATTTGAAGGTTACCCTGGA AGAAGAAACATCTACAGAATTAGCATTGGAAACTCTCCTCCGAGCAAGAAGTGTGTTACTTGCCATCTAA GGAAAGAAAGGTGCCAATATTACACAGCAAGTTTCAGCTACAAAGCCAAGTACTATGCACTCGTCTGCTA TGGCCCTGGCCTCCCCATTTCCACCCTCCATGATGGCCGCACAGACCAAGAAATACAAGTATTAGAAGAA AACAAAGAACTGGAAAATTCTCTGAGAAATATCCAGCTGCCTAAAGTGGAGATTAAGAAGCTCAAAGACG GGGGACTGACTTTCTGGTACAAGATGATTCTGCCTCCTCAGTTTGACAGATCAAAGAAGTACCCTTTGCT AATTCAAGTGTATGGTGGTCCTTGTAGCCAGAGTGTTAAGTCTGTGTTTGCTGTTAATTGGATAACTTAT CTCGCAAGTAAGGAGGGGATAGTCATTGCCCTGGTAGATGGTCGGGGCACTGCTTTCCAAGGTGACAAAT TCCTGCATGCCGTGTATCGAAAACTGGGTGTATATGAAGTTGAGGACCAGCTCACAGCTGTCAGAAAATT CATAGAAATGGGTTTCATTGATGAAGAAAGAATAGCCATATGGGGCTGGTCCTACGGAGGTTATGTTTCA TCCCTGGCCCTTGCATCTGGAACTGGTCTTTTCAAATGTGGCATAGCAGTGGCTCCAGTCTCCAGCTGGG AATATTACGCATCTATCTACTCAGAGAGATTCATGGGCCTCCCAACAAAGGACGACAATCTCGAACACTA TAAAAATTCAACTGTGATGGCAAGAGCAGAATATTTCAGAAATGTAGACTATCTTCTCATCCACGGAACA GCAGATGATAATGTGCACTTTCAGAACTCAGCACAGATTGCTAAAGCTTTGGTTAATGCACAAGTGGATT TCCAGGCGATGTGGTACTCTGACCAGAACCATGGTATATTATCTGGGCGCTCCCAGAATCATTTATATAC CCACATGACGCACTTCCTCAAGCAATGCTTTTCTTTATCAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210517 representing NM_007986
Red=Cloning site Green=Tags(s) MKTWLKTVFGVTTLAALALVVICIVLRPSRVYKPEGNTKRALTLKDILNGTFSYKTYFPNWISEQEYLHQ SEDDNIVFYNIETRESYIILSNSTMKSVNATDYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLQNGEF VRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKY ALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEV PVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGF FVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPG RRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEE NKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITY LASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVS SLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGT ADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGILSGRSQNHLYTHMTHFLKQCFSLSD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007986 |
| ORF Size | 2283 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007986.2, NP_032012.1 |
| RefSeq Size | 2695 bp |
| RefSeq ORF | 2286 bp |
| Locus ID | 14089 |
| UniProt ID | P97321 |
| Gene Summary | This gene belongs to the serine protease family. The encoded protein is an inducible cell-surface bound glycoprotein specifically expressed in tumor-associated fibroblasts and pericytes of epithelial tumors and has protease and gelatinase activity. The protein plays a role in remodeling of the extracellular matrix (ECM) and may affect tumorigenesis and tissue repair. Alternately spliced transcript variants of this gene are described in the literature (PMID 9139873), but the full-length sequence of these variants is not available. [provided by RefSeq, Apr 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210517 | Fap (Myc-DDK-tagged) - Mouse fibroblast activation protein (Fap) |
CNY 5,584.00 |
|
| MR210517L1 | Lenti ORF clone of Fap (Myc-DDK-tagged) - Mouse fibroblast activation protein (Fap) |
CNY 7,984.00 |
|
| MR210517L2 | Lenti ORF clone of Fap (mGFP-tagged) - Mouse fibroblast activation protein (Fap) |
CNY 7,984.00 |
|
| MR210517L3 | Lenti ORF clone of Fap (Myc-DDK-tagged) - Mouse fibroblast activation protein (Fap) |
CNY 7,984.00 |
|
| MR210517L4 | Lenti ORF clone of Fap (mGFP-tagged) - Mouse fibroblast activation protein (Fap) |
CNY 7,984.00 |
