Sema3a (BC057588) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	SemD, SEMA1, coll-1, Hsema-I
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210565 representing BC057588 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGGTTCACTGGGATTGCCTGTCTTTTCTGGGGTGTATTACTTACAGCCAGAGCAAACTATGCAA ACGGAAAGAACAATGTGCCAAGACTGAAATTATCGTACAAAGAAATGTTGGAATCCAACAATGTGATCAC TTTTAATGGCTTGGCCAACAGCTCCAGTTACCACACCTTCCTTCTGGATGAAGAACGGAGTAGACTATAT GTTGGAGCAAAAGATCATATATTTTCATTCAACTTGGTGAACATTAAAGATTTTCAAAAGATTGTGTGGC CAGTATCTTACACAAGGAGAGATGAATGCAAATGGGCTGGAAAAGATATCCTGAAAGAATGTGCCAATTT CATCAAGGTCCTGGAGGCTTATAATCAGACTCACTTGTATGCCTGTGGAACTGGGGCTTTCCATCCAATC TGCACCTATATTGAAGTTGGACATCATCCTGAGGACAACATTTTTAAGCTGCAGGACTCACATTTTGAAA ACGGTCGTGGGAAGAGCCCTTATGATCCCAAACTACTGACTGCCTCTCTTCTAATAGACGGTGAGTTGTA CTCTGGAACTGCTGCGGACTTCATGGGACGGGACTTCGCTATCTTCAGAACACTGGGGCACCATCACCCC ATCAGGACGGAGCAGCATGACTCCCGGTGGCTCAATGATCCTAGATTCATCAGTGCCCATCTCATCCCAG AGAGTGACAACCCTGAAGATGACAAAGTATATTTTTTCTTCCGAGAAAATGCAATAGACGGAGAACACTC TGGAAAAGCCACTCATGCTAGAATAGGTCAGATATGCAAGAATGACTTTGGTGGACACAGAAGTCTTGTG AATAAATGGACAACATTCCTAAAAGCACGCCTGATTTGCTCTGTGCCCGGTCCCAATGGCATTGACACCC ATTTTGATGAATTGCAGGATGTATTCCTAATGAACTCTAAAGATCCTAAAAATCCGATCGTCTATGGAGT GTTCACAACATCAAGCAACATCTTTAAGGGATCTGCTGTGTGCATGTACAGCATGAGTGATGTAAGAAGG GTGTTCCTTGGTCCATATGCTCACAGAGATGGTCCCAACTATCAGTGGGTGCCTTACCAAGGAAGAGTCC CTTATCCACGGCCAGGAACTTGTCCCAGTAAAACATTTGGCGGATTTGACTCCACAAAGGACCTTCCTGA TGATGTCATAACTTTTGCAAGAAGTCATCCAGCCATGTACAACCCAGTGTTTCCTATAAATAATCGCCCG ATCATGATCAAAACAGATGTAAATTATCAGTTCACACAAATTGTTGTAGACCGAGTGGATGCAGAAGATG GCCAGTATGATGTTATGTTCATCGGAACAGATGTTGGAACCGTTCTTAAAGTGGTTTCAGTCCCCAAGGA GACTTGGCATGACCTAGAAGAAGTTCTTCTGGAAGAAATGACCGTCTTCCGGGAACCAACAACTATTTCG GCAATGGAGCTTTCTACTAAACAGCAACAGCTGTACATTGGCTCAACTGCGGGAGTGGCACAGCTTCCTC TACACCGCTGTGACATCTATGGCAAAGCCTGTGCAGAATGCTGCCTCGCTCGGGACCCTTACTGTGCCTG GGATGGGTCCTCATGCTCACGCTATTTTCCTACTGCAAAGAGGCGCACAAGACGACAAGATATAAGGAAT GGAGACCCACTGACTCACTGCTCTGACTTGCAGCACCATGATAATCATCATGGGCCCAGCCTTGAAGAGA GAATCATCTATGGAGTGGAAAACAGTAGTACATTCTTGGAATGCAGTCCGAAGTCACAGAGAGCCTTGGT ATATTGGCAATTTCAGAGGAGAAATGAAGATCGAAAAGAGGAGATCAGAATGGGTGATCATATCATCAGG ACAGAACAAGGGCTCCTGCTCCGTAGCCTGCAGAAGAAGGATTCAGGCAATTACCTGTGTCACGCTGTGG AACACGGATTCATGCAAACTCTTCTTAAGGTAACCCTGGAAGTCATTGACACAGAACATTTGGAAGAACT TCTTCATAAAGATGACGATGGAGATGGCTCTAAGATAAAAGAAATGTCGAGCAGCATGACGCCCAGCCAG AAAGTCTGGTACCGAGACTTCATGCAGCTCATTAACCACCCCAACCTGAACACGATGGATGAGTTCTGTG AACAAGTGTGGAAAAGGGACCGAAAGCAACGCCGACAAAGGCCGGGGCACTCTCAAGGGAGCAGCAACAA GTGGAAGCACATGCAAGAGAGCAAGAAAGGTAGAAACAGGAGGACCCACGAGTTTGAGCGGGCACCCAGA AGTGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210565 representing BC057588 Red=Cloning site Green=Tags(s) MGWFTGIACLFWGVLLTARANYANGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLY VGAKDHIFSFNLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLEAYNQTHLYACGTGAFHPI CTYIEVGHHPEDNIFKLQDSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHP IRTEQHDSRWLNDPRFISAHLIPESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLV NKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNSKDPKNPIVYGVFTTSSNIFKGSAVCMYSMSDVRR VFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPINNRP IMIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSVPKETWHDLEEVLLEEMTVFREPTTIS AMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSSCSRYFPTAKRRTRRQDIRN GDPLTHCSDLQHHDNHHGPSLEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEDRKEEIRMGDHIIR TEQGLLLRSLQKKDSGNYLCHAVEHGFMQTLLKVTLEVIDTEHLEELLHKDDDGDGSKIKEMSSSMTPSQ KVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHSQGSSNKWKHMQESKKGRNRRTHEFERAPR SV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC057588
ORF Size	2316 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057588, AAH57588
RefSeq Size	4013 bp
RefSeq ORF	2318 bp
Locus ID	20346
Gene Summary	Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty (By similarity).[UniProtKB/Swiss-Prot Function]

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