Pfkl (NM_008826) Mouse Tagged ORF Clone
CAT#: MG210614
- TrueORF®
Pfkl (tGFP-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl)
ORF Plasmid: DDK
"NM_008826" in other vectors (4)
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CNY 7,510.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AA407869; ATP-PFK; PFK-B; PFK-L |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210614 representing NM_008826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACCGTGGACCTGGAGAAACTGCGGATGTCGGGGGCTGGCAAGGCCATTGGAGTGCTGACCAGCG GCGGTGATGCGCAAGGTATGAATGCTGCTGTCAGGGCTGTGACCCGTATGGGCATATATGTGGGGGCCAA AGTCTTCCTCATCTATGAGGGCTACGAGGGCCTTGTGGAAGGAGGCGAGAACATCAAGCCAGCCAACTGG CTCAGCGTTTCCAATATCATCCAGCTGGGTGGCACCATTATTGGCAGTGCCCGCTGTAAGGCCTTCACTA CGAGGGAAGGCCGCCTGGCTGCAGCCTACAATCTGCTCCAACACGGCATCACCAACCTGTGTGTCATCGG TGGCGATGGCAGCCTCACGGGGGCCAACATCTTCCGCAACGAGTGGGGCAGCTTGCTGGAGGAGCTGGTG AAGGAAGGCAAGATCTCAGAGTCCACAGCTCAGAACTACGCACACTTGACCATCGCCGGTCTGGTGGGCT CCATCGATAATGACTTCTGCGGCACTGACATGACCATTGGCACAGACTCAGCCCTGCACCGCATTATGGA GGTCATTGACGCCATCACTACCACTGCCCAAAGTCACCAGAGGACCTTTGTTTTGGAGGTGATGGGACGG CACTGCGGGTACCTGGCGCTGGTGTCTGCGCTGGCTTCCGGGGCTGATTGGCTATTCATTCCTGAAGCGC CCCCTGAGGATGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGCGAGACTCGGAGCCGAGGCTCTCGGCT GAACATCATCATCATCGCAGAGGGCGCCATTGACCGGCATGGAAAGCCTATCTCATCCAGCTACGTGAAG GATCTGGTGGTTCAGAGGCTGGGCTTCGATACACGAGTGACTGTGCTGGGTCATGTACAGCGAGGAGGGA CGCCTTCAGCCTTCGACCGAATCCTGAGTAGCAAGATGGGTATGGAGGCCGTGATGGCGCTGCTAGAGGC CACGCCTGACACGCCGGCCTGTGTGGTCAGCCTCTCCGGGAACCAGTCTGTGAGGCTGCCTCTCATGGAG TGTGTGCAAGTGACAAAGGACGTGCAGAAGGCCATGGACGAGGAGAGGTTTGACGAGGCCATCCAGCTCC GTGGCAGGAGCTTTGAGAACAACTGGAAAATTTACAAGCTCCTTGCCCACCAGAAGGTGTCTAAAGAGAA GTCCAACTTCTCCCTGGCCATCCTGAATGTGGGGGCTCCAGCTGCTGGCATGAATGCAGCTGTGCGCTCC GCAGTGCGCACCGGTATCTCCGAGGGACACACGGTATACATCGTGCATGATGGCTTTGAGGGTCTGGCCA AGGGTCAGGTGCAAGAAGTGGGCTGGCATGATGTGGCAGGCTGGCTGGGACGTGGTGGCTCGATGCTGGG GACCAAGAGGACACTGCCCAAGCCCCATCTGGAGGCCATTGTGGAGAATCTCCGCACCTACAACATCCAC GCCCTGCTGGTGATTGGTGGCTTTGAGGCCTACGAGGGTGTGCTGCAGCTGGTGGAGGCCCGGGGGCGCT ACGAGGAACTCTGCATTGTCATGTGCGTCATCCCAGCCACCATCAGCAACAATGTGCCTGGCACTGACTT CAGCCTGGGCTCAGACACGGCTGTCAACGCTGCAATGGAGAGTTGTGATCGCATCAAGCAGTCAGCCTCA GGGACAAAGCGGCGTGTGTTCATTGTAGAGACCATGGGGGGCTACTGTGGCTACCTGGCCACTGTGACCG GCATTGCTGTGGGTGCCGATGCTGCCTACGTCTTTGAGGACCCTTTCAACATCCATGACTTAAAGGCCAA TGTGGAGCATATGACAGAGAAGATGAAGACAGACATCCAGAGGGGACTGGTGCTCCGGAACGAGAAGTGT CACGAACACTACACCACAGAGTTCCTATACAACCTGTACTCCTCAGAAGGCAGGGGCGTGTTTGACTGCA GGACCAATGTGCTGGGCCACTTACAGCAGGGTGGTGCTCCAACCCCTTTCGACCGGAACTATGGGACCAA ACTGGGGGTGAAGGCCATGTTGTGGGTGTCTGAGAAGCTACGTGATGTCTACCGTAAAGGGCGGGTGTTT GCCAATGCTCCAGACTCAGCCTGTGTGATCGGCCTGCGGAAGAAGGTAGTGGCCTTCAGTCCGGTCACAG AACTCAAGAAAGAGACTGATTTTGAGCACCGCATGCCCCGGGAGCAGTGGTGGCTGAATCTGCGGCTGAT GCTGAAGATGCTGGCACACTATCGCATCAGCATGGCAGACTATGTGTCTGGGGAGCTGGAGCACGTCACA CGCCGCACCTTGAGCATAGACAAGGGTTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210614 representing NM_008826
Red=Cloning site Green=Tags(s) MATVDLEKLRMSGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKPANW LSVSNIIQLGGTIIGSARCKAFTTREGRLAAAYNLLQHGITNLCVIGGDGSLTGANIFRNEWGSLLEELV KEGKISESTAQNYAHLTIAGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGR HCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRHGKPISSSYVK DLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVSLSGNQSVRLPLME CVQVTKDVQKAMDEERFDEAIQLRGRSFENNWKIYKLLAHQKVSKEKSNFSLAILNVGAPAAGMNAAVRS AVRTGISEGHTVYIVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKPHLEAIVENLRTYNIH ALLVIGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSAS GTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIHDLKANVEHMTEKMKTDIQRGLVLRNEKC HEHYTTEFLYNLYSSEGRGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWVSEKLRDVYRKGRVF ANAPDSACVIGLRKKVVAFSPVTELKKETDFEHRMPREQWWLNLRLMLKMLAHYRISMADYVSGELEHVT RRTLSIDKGF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008826 |
ORF Size | 2340 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008826.2, NP_032852.2 |
RefSeq Size | 3350 bp |
RefSeq ORF | 2343 bp |
Locus ID | 18641 |
UniProt ID | P12382 |
Gene Summary | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (PubMed:26194095).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203910 | Pfkl (untagged) - Mouse phosphofructokinase, liver, B-type (Pfkl), (10ug) |
CNY 5,720.00 |
|
MR210614 | Pfkl (Myc-DDK-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) |
CNY 5,720.00 |
|
MR210614L3 | Lenti ORF clone of Pfkl (Myc-DDK-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) |
CNY 8,740.00 |
|
MR210614L4 | Lenti ORF clone of Pfkl (mGFP-tagged) - Mouse phosphofructokinase, liver, B-type (Pfkl) |
CNY 8,740.00 |