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Rbsn (NM_030081) Mouse Tagged ORF Clone
CAT＃: MG210635
- TrueORF®
Zfyve20 (tGFP-tagged) - Mouse zinc finger, FYVE domain containing 20 (Zfyve20)
ORF Plasmid: DDK
"NM_030081" in other vectors (4)
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CNY 8,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5330426D11Rik; Zfyve20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210635 representing NM_030081
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCTCTTGATGACCCAGGGGAGGTGAGGGAAGGCTTCCTGTGCCCTTTGTGCCTGAAGGACCTTC AGTCTTTCTATCAACTTCAGTCACATTATGAGGAAGAACACTTGGAAGATCGTGATGTCAAAGGGCAAAT TAAAAATCTTGTTCAGAAGGCTAGGAAAGCAAAGAACAAGTTGTTGAAACGAGAAGGAGATGATCGAGTG GAACCAGGGACCCAAGGATATGAATCATTCAGTTATGGTGGGGTTGATCCTTACATGTGGGAACCCCAAG AGCTTGGTGCTATGAGAAGCCATCTTTCTGACTTCAAAAAACATCGTGCTGCAAGAATTGACCACTATGT TGTTGAAGTCAATAAATTAATCATCAGGTTGGAAAAGCTTACTGCATTTGACAGAACAAATACTGAGACT TCAAAGATTAGAGCAATAGAAAAGTCCGTGGTGCCTTGGGTCAATGACCAGGATGTCCCCTTCTGTCCAG ACTGTGGGAATAAATTCAGCATCCGGAACCGCCGTCACCACTGCCGCCTCTGTGGGTCTATCATGTGCAA GAAGTGTATGGAGCTCATCGGCCTGCCCTTAGCACATAAGCTCACCAGTGCCAGCAAGGACTCTCTGAGC ACCCACACCAGCCCCAGCCAGTCTCCTAACAGTGTCCATGGCTCCCGCCGGGGCAGCATCAGCAGCATGA GCAGTGTCAGCTCAGTCCTAGACGAGAAGGATGATGACCGCATCCGCTGCTGCACACACTGCAAGGACAA GCTGCTCAAGCGAGAGCAGCAGATGGACGAGAAGGAGCACACGCCTGACATCGTGAAGCTCTACGAGAAA CTTCGGCTTTGCATGGAGAAAGTTGACCAGAAAGCTCCCGAATACATCAGGATGGCAGCATCTTTAAATG CTGGGGAGACGACCTACAATCTGGAGCATGCCAATGACCTTCGAGTGGAAGTGCAGAAAGTATATGAGCT AATAGACGCTTTAAGTAAGAAGATCTTAACCTTGGGCTTGAACCAAGACCCTTCACCACATCCAAACACT TTGCGGCTACAGAGAATGATCAGATACTCAGCTACATTATTTGTGCAGGAAAAGTTACTGGGTTTGATGT CACTGCCAACCAAAGAACAGTTTGAAGAACTGAAAAAGAAAAGGAAGCAGGACCTGGAGCAGAAGAGGAC CGTGGAGAGACAGGCTGCTCTGGAGTCACGGCGGAAGCTTGAGGAAAGGCAGAGTGGTTTGGCATCTCAT ACAGCCAATGGGGACGTGAGGTCTCTTCGAGGCATCCCTCCCCCCTTGCGAAAGGCTGAGGGCTGGCTCC CACTGTCAGAAGGTCAGGGACAGAGTGAAGATCCTGACCCTCTCCTCCAGCAGATCTATAACATTACATC GTTCATCAGGCAGGCCAAGGCTGCAGGCCGCACAGATGAGGTGCGCACACTTCAGGAGAACCTGCGGCAG CTGCAGGATGAGTATGACCAGCAGCAGACTGAGAAGGCCATTGAGCTGTCCCGGAAGCAGGCCGAGGAGG AGGAGCTGCAGCGGGAGCAGCTACAGATGCTCCGCAAGCGAGAGCTTGAACGAGAACAGGAGCAGTTCCT GGCAGCATCCCTGCAAACACGGACTCGTGTTCTAGAACTCCGAGAAGTTATACCCTTCCAATTGGAGGCC AGTCGAGGGCCTCACATTGATCTCTCCTATTCCTTGGATCAGGACTCCTCCCCAGTTCAGAGCAGCACAG CTCCTGATATCCTTACACCTGGCTCAGCTCTAGCACCCATGCACTTGTGGTCTGGGCCCCCAGCCCTTGG CCAGGAAACACTCCCTCAGAGCACCATGTCACAGCAAAGTGATAAGGCCTCACTCAACCCGTTTGATGAA GACGACCTCTCCAGCCCTACAGAGGGTGCTATCAGCCCTGCTGCTGTAGAGGCTTTCCTGGGCCCTCCAG CTGCTGTCACCAAAGAATACAATCCTTTTGAGGAAGATGCTGAGGAAGAGGAGGTGGCAGAGTTGGGGGC AGGAAATCCCTTCACCGACCCAGACAGTCCAGCACCCAACCCTTTTGATGAGGATGACGGTCCCAGGCCT GCCAGTCCAGCCGCCCCTGGAAACCCTTTTGAAGAGTGCCCCTCCACCAACCCCTTCGAGGTGGACAGTG ATAGTGGCATGGAGGCCGAGGAACACATCGAGGAGGAGCTGCTCCTGCAGCAAATTGACAACATCAAGGC TTACATCTTTGATGCCAAGCAGTGTGGGCGAATGGACGAGGTTGAGGTTCTAACGGAGAACCTCCGGGAG CTGAAGTGCACCCTGGCTAAGCAGAAGGGGGCCCCTAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210635 representing NM_030081
Red=Cloning site Green=Tags(s) MASLDDPGEVREGFLCPLCLKDLQSFYQLQSHYEEEHLEDRDVKGQIKNLVQKARKAKNKLLKREGDDRV EPGTQGYESFSYGGVDPYMWEPQELGAMRSHLSDFKKHRAARIDHYVVEVNKLIIRLEKLTAFDRTNTET SKIRAIEKSVVPWVNDQDVPFCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELIGLPLAHKLTSASKDSLS THTSPSQSPNSVHGSRRGSISSMSSVSSVLDEKDDDRIRCCTHCKDKLLKREQQMDEKEHTPDIVKLYEK LRLCMEKVDQKAPEYIRMAASLNAGETTYNLEHANDLRVEVQKVYELIDALSKKILTLGLNQDPSPHPNT LRLQRMIRYSATLFVQEKLLGLMSLPTKEQFEELKKKRKQDLEQKRTVERQAALESRRKLEERQSGLASH TANGDVRSLRGIPPPLRKAEGWLPLSEGQGQSEDPDPLLQQIYNITSFIRQAKAAGRTDEVRTLQENLRQ LQDEYDQQQTEKAIELSRKQAEEEELQREQLQMLRKRELEREQEQFLAASLQTRTRVLELREVIPFQLEA SRGPHIDLSYSLDQDSSPVQSSTAPDILTPGSALAPMHLWSGPPALGQETLPQSTMSQQSDKASLNPFDE DDLSSPTEGAISPAAVEAFLGPPAAVTKEYNPFEEDAEEEEVAELGAGNPFTDPDSPAPNPFDEDDGPRP ASPAAPGNPFEECPSTNPFEVDSDSGMEAEEHIEEELLLQQIDNIKAYIFDAKQCGRMDEVEVLTENLRE LKCTLAKQKGAPN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030081 |
| ORF Size | 2349 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030081.3 |
| RefSeq Size | 5242 bp |
| RefSeq ORF | 2352 bp |
| Locus ID | 78287 |
| UniProt ID | Q80Y56 |
| Gene Summary | Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3). Plays a role in the recycling of transferrin receptor to the plasma membrane (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202719 | Zfyve20 (untagged) - Mouse zinc finger, FYVE domain containing 20 (Zfyve20), (10ug) |
CNY 5,744.00 |
|
| MR210635 | Zfyve20 (Myc-DDK-tagged) - Mouse zinc finger, FYVE domain containing 20 (Zfyve20) |
CNY 7,790.00 |
|
| MR210635L3 | Lenti ORF clone of Zfyve20 (Myc-DDK-tagged) - Mouse zinc finger, FYVE domain containing 20 (Zfyve20) |
CNY 9,690.00 |
|
| MR210635L4 | Lenti ORF clone of Zfyve20 (mGFP-tagged) - Mouse zinc finger, FYVE domain containing 20 (Zfyve20) |
CNY 9,690.00 |
