Dnajc10 (BC033461) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	ERdj5, JPDI
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210690 representing BC033461 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGTCTGGTTAAACAAAGATGACTTTATCAGAGACTTGAAGAGGATCAGTCTCTGTCTTCTGATAC TGTACGTGGTCGTTGTAGTGGGCACAGATCAGAATTTTTACAGTTTACTTGGAGTATCTAAAACTGCAAG CAGTAGAGAAATAAGACAAGCCTTTAAGAAATTAGCACTGAAGTTACATCCTGATAAAAACCCGAATAAC CCAAATGCACATGGTGATTTTTTAAAAATAAATAGAGCATATGAAGTACTCAAAGATGAAGATCTACGGA AAAAGTATGACAAATATGGAGAAAAGGGACTTGAAGATAATCAAGGAGGCCAGTATGAGAGCTGGAGCTA CTACCGTTACGATTTCGGTATTTACGATGATGATCCTGAAATCATAACATTAGAAAGAAGAGAATTTGAT GCTGCTGTCAACTCTGGAGAACTGTGGTTTGTAAACTTTTACTCCCCAGGATGTTCGCATTGCCATGATT TAGCCCCCACTTGGAGAGAATTTGCTAAAGAAGTGGATGGGTTGCTTCGAATCGGAGCTGTCAACTGTGG TGATGATCGGATGCTCTGCCGAATGAAAGGAGTTAACAGCTACCCCAGCCTCTTCATCTTTAGGTCTGGA ATGGCTGCAGTGAAGTACAATGGAGATCGGTCAAAGGAGAGCTTAGTGGCCTTCGCCATGCAGCACGTCA GGAGCACAGTGACAGAGCTGTCAACAGGAAATTTTGTTAATGCCATAGAAACTGCTTTTGCTGCTGGTGT TGGCTGGCTGATCACATTCTGCTCTAAAGGAGAAGATTGTTTGACGTCACAGACCCGACTCAGGCTTAGT GGCATGTTGGATGGCCTTGTTAACGTGGGATGGGTGGACTGTGACGCCCAGGACAGCCTCTGCAAGAGCC TGGACACCACAGCCAGCACCACGGCTTATTTCCCTCCTGGAGCCACTTTGAATGACAGGGGAAAATCCAG TGTGTTGTTTCTTAATTCATTGGATGCTAAGGAAATATACATGGAAATCATACATAATCTTCCAGACTTT GAACTGCTATCAGCAAATCAACTAGAGGATCGTTTGGCACATCATCGCTGGCTTGTATTTTTTCATTTTG GAAAAAATGAAAACGCAAATGATCCTGAGTTGAAAAAACTGAAAACTCTACTTAAAAATGAGCATATTCA AGTTGGAAGGTTTGATTGCTCTTCTGCACCGGGCATCTGCTCTGATCTTTATGTCTTCCAGCCCTGCCTA GCAGTGTTTAAAGGACAAGGAACCAAAGAATATGAAACTCATCATGGAAAGAAGATTCTATACGATATAC TTGCTTTTGCCAAAGAAAGTGTTAATTCTCATGTTACCACACTTGGACCTCAGAACTTCCCTGCAAGTGA CAAGGAACCATGGCTTGTTGACTTCTTTGCCCCTTGGTGTCCGCCATGTCGTGCGCTTTTGCCGGAGTTA CGGAAAGCATCAACACTGCTCTACGGTCAACTCAAAGTTGGTACCTTAGACTGTACAATTCATGAAGGAC TCTGCAACATGTATAACATCCAAGCTTATCCAACGACAGTGGTATTCAATCAGTCCAGTATTCATGAGTA TGAAGGACATCACTCTGCGGAACAGATCTTGGAGTTCATAGAGGATCTCAGAAACCCTTCAGTGGTCTCT CTTACACCCAGCACTTTCAATGAGCTGGTTAAACAGAGAAAGCATGATGAAGTCTGGATGGTCGATTTCT ATTCTCCATGGTGTCATCCCTGTCAGGTCTTGATGCCAGAATGGAAACGCATGGCCCGGACGTTAACTGG ACTGATCAATGTAGGCAGTGTGGACTGCCAACAGTATCATTCTTTTTGTACTCAAGAAAATGTTCAAAGA TACCCCGAGATAAGATTTTATCCCCAAAAGTCAAGTAAAGCCTATCAGTATCATAGTTACAATGGCTGGA ATAGGGATGCGTATTCCCTGAGAAGCTGGGGTCTTGGATTTTTGCCCCAAGCATCCATAGATTTAACACC TCAGACTTTCAATGAAAAAGTTCTACAAGGGAAAACTCACTGGGTGGTTGATTTCTATGCTCCTTGGTGT GGACCTTGCCAGAATTTTGCTCCTGAGTTTGAACTCTTGGCTAGGATGATCAAAGGAAAAGTGAGAGCCG GGAAAGTGGACTGTCAGGCTTACCCCCAGACGTGCCAGAAAGCCGGCATCAAAGCCTACCCCAGTGTCAA ACTGTACCAGTACGAGAGAGCAAAGAAAAGTATTTGGGAAGAGCAAATAAATTCCAGAGATGCAAAAACT ATTGCTGCCTTAATTTATGGAAAATTGGAAACGCTCCAAAGCCAAGTAAAAAGAAATAAGGATGAGCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210690 representing BC033461 Red=Cloning site Green=Tags(s) MGVWLNKDDFIRDLKRISLCLLILYVVVVVGTDQNFYSLLGVSKTASSREIRQAFKKLALKLHPDKNPNN PNAHGDFLKINRAYEVLKDEDLRKKYDKYGEKGLEDNQGGQYESWSYYRYDFGIYDDDPEIITLERREFD AAVNSGELWFVNFYSPGCSHCHDLAPTWREFAKEVDGLLRIGAVNCGDDRMLCRMKGVNSYPSLFIFRSG MAAVKYNGDRSKESLVAFAMQHVRSTVTELSTGNFVNAIETAFAAGVGWLITFCSKGEDCLTSQTRLRLS GMLDGLVNVGWVDCDAQDSLCKSLDTTASTTAYFPPGATLNDRGKSSVLFLNSLDAKEIYMEIIHNLPDF ELLSANQLEDRLAHHRWLVFFHFGKNENANDPELKKLKTLLKNEHIQVGRFDCSSAPGICSDLYVFQPCL AVFKGQGTKEYETHHGKKILYDILAFAKESVNSHVTTLGPQNFPASDKEPWLVDFFAPWCPPCRALLPEL RKASTLLYGQLKVGTLDCTIHEGLCNMYNIQAYPTTVVFNQSSIHEYEGHHSAEQILEFIEDLRNPSVVS LTPSTFNELVKQRKHDEVWMVDFYSPWCHPCQVLMPEWKRMARTLTGLINVGSVDCQQYHSFCTQENVQR YPEIRFYPQKSSKAYQYHSYNGWNRDAYSLRSWGLGFLPQASIDLTPQTFNEKVLQGKTHWVVDFYAPWC GPCQNFAPEFELLARMIKGKVRAGKVDCQAYPQTCQKAGIKAYPSVKLYQYERAKKSIWEEQINSRDAKT IAALIYGKLETLQSQVKRNKDEL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC033461
ORF Size	2381 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033461, AAH33461
RefSeq Size	3616 bp
RefSeq ORF	2381 bp
Locus ID	66861
Gene Summary	Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.[UniProtKB/Swiss-Prot Function]

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