Dnajc10 (BC033461) Mouse Tagged ORF Clone

CAT#: MG210690

  • TrueORF®

Dnajc10 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 10 (cDNA clone MGC:28281 IMAGE:4010341)



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Mouse Protein / Lysate Request

CNY 7,700.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms ERdj5, JPDI
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210690 representing BC033461
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGTCTGGTTAAACAAAGATGACTTTATCAGAGACTTGAAGAGGATCAGTCTCTGTCTTCTGATAC
TGTACGTGGTCGTTGTAGTGGGCACAGATCAGAATTTTTACAGTTTACTTGGAGTATCTAAAACTGCAAG
CAGTAGAGAAATAAGACAAGCCTTTAAGAAATTAGCACTGAAGTTACATCCTGATAAAAACCCGAATAAC
CCAAATGCACATGGTGATTTTTTAAAAATAAATAGAGCATATGAAGTACTCAAAGATGAAGATCTACGGA
AAAAGTATGACAAATATGGAGAAAAGGGACTTGAAGATAATCAAGGAGGCCAGTATGAGAGCTGGAGCTA
CTACCGTTACGATTTCGGTATTTACGATGATGATCCTGAAATCATAACATTAGAAAGAAGAGAATTTGAT
GCTGCTGTCAACTCTGGAGAACTGTGGTTTGTAAACTTTTACTCCCCAGGATGTTCGCATTGCCATGATT
TAGCCCCCACTTGGAGAGAATTTGCTAAAGAAGTGGATGGGTTGCTTCGAATCGGAGCTGTCAACTGTGG
TGATGATCGGATGCTCTGCCGAATGAAAGGAGTTAACAGCTACCCCAGCCTCTTCATCTTTAGGTCTGGA
ATGGCTGCAGTGAAGTACAATGGAGATCGGTCAAAGGAGAGCTTAGTGGCCTTCGCCATGCAGCACGTCA
GGAGCACAGTGACAGAGCTGTCAACAGGAAATTTTGTTAATGCCATAGAAACTGCTTTTGCTGCTGGTGT
TGGCTGGCTGATCACATTCTGCTCTAAAGGAGAAGATTGTTTGACGTCACAGACCCGACTCAGGCTTAGT
GGCATGTTGGATGGCCTTGTTAACGTGGGATGGGTGGACTGTGACGCCCAGGACAGCCTCTGCAAGAGCC
TGGACACCACAGCCAGCACCACGGCTTATTTCCCTCCTGGAGCCACTTTGAATGACAGGGGAAAATCCAG
TGTGTTGTTTCTTAATTCATTGGATGCTAAGGAAATATACATGGAAATCATACATAATCTTCCAGACTTT
GAACTGCTATCAGCAAATCAACTAGAGGATCGTTTGGCACATCATCGCTGGCTTGTATTTTTTCATTTTG
GAAAAAATGAAAACGCAAATGATCCTGAGTTGAAAAAACTGAAAACTCTACTTAAAAATGAGCATATTCA
AGTTGGAAGGTTTGATTGCTCTTCTGCACCGGGCATCTGCTCTGATCTTTATGTCTTCCAGCCCTGCCTA
GCAGTGTTTAAAGGACAAGGAACCAAAGAATATGAAACTCATCATGGAAAGAAGATTCTATACGATATAC
TTGCTTTTGCCAAAGAAAGTGTTAATTCTCATGTTACCACACTTGGACCTCAGAACTTCCCTGCAAGTGA
CAAGGAACCATGGCTTGTTGACTTCTTTGCCCCTTGGTGTCCGCCATGTCGTGCGCTTTTGCCGGAGTTA
CGGAAAGCATCAACACTGCTCTACGGTCAACTCAAAGTTGGTACCTTAGACTGTACAATTCATGAAGGAC
TCTGCAACATGTATAACATCCAAGCTTATCCAACGACAGTGGTATTCAATCAGTCCAGTATTCATGAGTA
TGAAGGACATCACTCTGCGGAACAGATCTTGGAGTTCATAGAGGATCTCAGAAACCCTTCAGTGGTCTCT
CTTACACCCAGCACTTTCAATGAGCTGGTTAAACAGAGAAAGCATGATGAAGTCTGGATGGTCGATTTCT
ATTCTCCATGGTGTCATCCCTGTCAGGTCTTGATGCCAGAATGGAAACGCATGGCCCGGACGTTAACTGG
ACTGATCAATGTAGGCAGTGTGGACTGCCAACAGTATCATTCTTTTTGTACTCAAGAAAATGTTCAAAGA
TACCCCGAGATAAGATTTTATCCCCAAAAGTCAAGTAAAGCCTATCAGTATCATAGTTACAATGGCTGGA
ATAGGGATGCGTATTCCCTGAGAAGCTGGGGTCTTGGATTTTTGCCCCAAGCATCCATAGATTTAACACC
TCAGACTTTCAATGAAAAAGTTCTACAAGGGAAAACTCACTGGGTGGTTGATTTCTATGCTCCTTGGTGT
GGACCTTGCCAGAATTTTGCTCCTGAGTTTGAACTCTTGGCTAGGATGATCAAAGGAAAAGTGAGAGCCG
GGAAAGTGGACTGTCAGGCTTACCCCCAGACGTGCCAGAAAGCCGGCATCAAAGCCTACCCCAGTGTCAA
ACTGTACCAGTACGAGAGAGCAAAGAAAAGTATTTGGGAAGAGCAAATAAATTCCAGAGATGCAAAAACT
ATTGCTGCCTTAATTTATGGAAAATTGGAAACGCTCCAAAGCCAAGTAAAAAGAAATAAGGATGAGCTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210690 representing BC033461
Red=Cloning site Green=Tags(s)

MGVWLNKDDFIRDLKRISLCLLILYVVVVVGTDQNFYSLLGVSKTASSREIRQAFKKLALKLHPDKNPNN
PNAHGDFLKINRAYEVLKDEDLRKKYDKYGEKGLEDNQGGQYESWSYYRYDFGIYDDDPEIITLERREFD
AAVNSGELWFVNFYSPGCSHCHDLAPTWREFAKEVDGLLRIGAVNCGDDRMLCRMKGVNSYPSLFIFRSG
MAAVKYNGDRSKESLVAFAMQHVRSTVTELSTGNFVNAIETAFAAGVGWLITFCSKGEDCLTSQTRLRLS
GMLDGLVNVGWVDCDAQDSLCKSLDTTASTTAYFPPGATLNDRGKSSVLFLNSLDAKEIYMEIIHNLPDF
ELLSANQLEDRLAHHRWLVFFHFGKNENANDPELKKLKTLLKNEHIQVGRFDCSSAPGICSDLYVFQPCL
AVFKGQGTKEYETHHGKKILYDILAFAKESVNSHVTTLGPQNFPASDKEPWLVDFFAPWCPPCRALLPEL
RKASTLLYGQLKVGTLDCTIHEGLCNMYNIQAYPTTVVFNQSSIHEYEGHHSAEQILEFIEDLRNPSVVS
LTPSTFNELVKQRKHDEVWMVDFYSPWCHPCQVLMPEWKRMARTLTGLINVGSVDCQQYHSFCTQENVQR
YPEIRFYPQKSSKAYQYHSYNGWNRDAYSLRSWGLGFLPQASIDLTPQTFNEKVLQGKTHWVVDFYAPWC
GPCQNFAPEFELLARMIKGKVRAGKVDCQAYPQTCQKAGIKAYPSVKLYQYERAKKSIWEEQINSRDAKT
IAALIYGKLETLQSQVKRNKDEL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC033461
ORF Size 2381 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC033461, AAH33461
RefSeq Size 3616 bp
RefSeq ORF 2381 bp
Locus ID 66861
Gene Summary Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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