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Kdm1a (NM_133872) Mouse Tagged ORF Clone
CAT＃: MG210741
- TrueORF®
Kdm1a (tGFP-tagged) - Mouse amine oxidase (flavin containing) domain 2 (Aof2)
ORF Plasmid: DDK
"NM_133872" in other vectors (3)
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CNY 8,740.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1810043O07Rik; AA408884; Aof2; D4Ertd478e; Kdm1; Lsd1; mKIAA0601 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210741 representing NM_133872
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACGGGGGCGGCGGGCGAGCGCACTCCCCGAAAGAAGGAGCCTCCGCGGGCCTCGCCGCCCGGGG GCCTAGCCGAGCCGCCGGGGTCTGCTGGGCCCCAGGCGGGGCCCACAGCCGGGCCCGGCTCCGCGACGCC CATGGAGACCGGAATAGCCGAGACCCCGGAGGGCCGACGGACCAGCCGGCGCAAGCGGGCCAAGGTAGAA TACAGAGAAATGGATGAAAGCTTGGCCAACCTCTCAGAAGATGAATATTATTCGGAAGAAGAAAGAAATG CTAAAGCAGAGAAGGAAAAGAAGCTTCCCCCACCACCTCCTCAAGCCCCACCTGAGGAAGAAAATGAAAG TGAGCCGGAAGAGCCGTCTGGTGTGGAGGGTGCAGCTTTTCAAAGCCGACTTCCCCATGACCGAATGACC TCTCAGGAAGCAGCCTGTTTCCCAGACATCATCAGTGGGCCTCAGCAGACACAGAAGGTTTTTCTGTTCA TCAGGAATCGCACATTGCAGTTATGGCTGGACAACTCAAAGATCCAGCTGACGTTTGAAGCCACTCTCCA GCAGCTGGAAGCGCCTTACAACAGCGATACTGTGCTTGTCCACCGAGTTCACAGTTACTTAGAGCGCCAT GGTCTTATCAACTTCGGCATCTACAAGAGGATAAAACCCTTACCAATTAAAAAGACAGGAAAGGTGATTA TTATAGGTTCAGGTGTTTCTGGCTTGGCAGCAGCTCGACAGCTACAGAGTTTTGGGATGGATGTCACACT TCTGGAAGCCAGGGATCGAGTAGGTGGACGAGTTGCTACATTTCGAAAAGGAAACTATGTAGCTGATCTT GGCGCCATGGTTGTAACAGGTCTTGGAGGGAATCCCATGGCTGTCGTCAGCAAACAAGTAAATATGGAAC TGGCCAAGATCAAGCAAAAATGCCCACTTTATGAAGCCAATGGACAAGCTGTTCCAAAAGAAAAAGATGA AATGGTAGAACAAGAATTTAACCGGTTGCTAGAAGCCACTTCTTACCTTAGTCACCAGTTAGACTTCAAC GTCCTCAATAATAAACCTGTATCCCTTGGCCAGGCATTGGAGGTTGTCATTCAGCTGCAAGAAAAGCATG TCAAAGATGAGCAGATTGAACATTGGAAGAAGATAGTGAAAACTCAGGAGGAGTTGAAAGAGCTTCTTAA TAAGATGGTAAATTTGAAGGAGAAAATTAAAGAGCTCCATCAGCAATACAAAGAAGCTTCAGAAGTGAAG CCGCCCAGAGATATCACAGCCGAGTTCCTGGTGAAGAGCAAGCACAGGGACCTGACTGCCCTCTGCAAGG AATATGATGAATTAGCTGAAACACAAGGAAAGCTAGAAGAAAAACTTCAAGAATTGGAAGCCAATCCCCC AAGTGATGTATACCTCTCATCAAGAGACAGACAAATACTTGACTGGCATTTTGCAAATCTTGAATTTGCC AACGCCACACCTCTCTCTACCCTCTCTCTTAAACATTGGGATCAGGATGATGACTTTGAGTTTACTGGAA GCCACCTGACAGTAAGGAATGGCTACTCATGTGTGCCTGTGGCTTTAGCTGAAGGCTTGGACATTAAACT GAACACAGCAGTGCGGCAGGTTCGCTACACAGCCTCAGGATGTGAAGTGATTGCTGTGAACACACGTTCC ACAAGTCAAACCTTTATTTATAAGTGTGATGCAGTTCTCTGTACACTTCCTTTGGGAGTGTTGAAGCAGC AGCCACCAGCTGTTCAGTTTGTGCCACCTCTTCCTGAGTGGAAAACATCTGCAGTCCAAAGGATGGGATT TGGCAACCTTAACAAGGTGGTGTTATGCTTTGACCGTGTGTTCTGGGACCCAAGTGTCAATTTGTTTGGG CACGTTGGCAGTACAACTGCTAGCAGGGGTGAGCTCTTCCTCTTCTGGAACCTATATAAAGCTCCAATAC TATTGGCCCTGGTAGCAGGAGAAGCTGCTGGCATTATGGAGAACATTAGTGATGATGTGATTGTCGGCCG GTGCCTGGCCATTCTCAAAGGGATTTTTGGCAGCAGTGCAGTCCCACAGCCCAAGGAAACTGTGGTATCT CGTTGGCGTGCTGATCCGTGGGCCCGGGGCTCCTATTCTTATGTGGCTGCAGGATCCTCTGGAAATGACT ATGATTTAATGGCTCAGCCGATCACTCCTGGCCCCTCAATTCCAGGTGCCCCACAGCCAATCCCAAGACT CTTCTTTGCTGGAGAACACACAATCCGGAACTACCCAGCTACAGTCCATGGTGCTCTGTTGAGTGGGCTT CGAGAAGCAGGAAGGATTGCCGACCAGTTTTTGGGAGCCATGTACACTTTGCCTCGTCAGGCCACACCAG GTGTCCCTGCACAGCAGTCCCCAAGTATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210741 representing NM_133872
Red=Cloning site Green=Tags(s) MATGAAGERTPRKKEPPRASPPGGLAEPPGSAGPQAGPTAGPGSATPMETGIAETPEGRRTSRRKRAKVE YREMDESLANLSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMT SQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNSKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERH GLINFGIYKRIKPLPIKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADL GAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFN VLNNKPVSLGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVK PPRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFA NATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRS TSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFG HVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVS RWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGL REAGRIADQFLGAMYTLPRQATPGVPAQQSPSM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133872 |
| ORF Size | 2409 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133872.1, NP_598633.1 |
| RefSeq Size | 2999 bp |
| RefSeq ORF | 2562 bp |
| Locus ID | 99982 |
| UniProt ID | Q6ZQ88 |
| Gene Summary | Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation (By similarity). Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210741 | Kdm1a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 1A (Kdm1a) |
CNY 8,824.00 |
|
| MR210741L3 | Lenti ORF clone of Kdm1a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 1A (Kdm1a) |
CNY 9,880.00 |
|
| MR210741L4 | Lenti ORF clone of Kdm1a (mGFP-tagged) - Mouse lysine (K)-specific demethylase 1A (Kdm1a) |
CNY 9,880.00 |
