Tlr6 (BC055366) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210761 representing BC055366 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAAAGTCCCTCTGGGATAGCCTCTGCAACATGAGCCAAGACAGAAAACCCATCGTGGGGAGTTTCC ACTTTGTTTGCGCCCTGGCCTTAATAGTCGGAAGCATGACCCCGTTCTCTAATGAACTTGAGTCTATGGT AGACTATTCAAACAGGAACCTTACTCATGTCCCCAAAGACCTGCCACCAAGAACAAAAGCCCTGAGTCTG TCTCAAAACTCTATATCTGAGCTTCGGATGCCTGATATCAGCTTTCTGTCAGAGCTGAGAGTTCTGAGAC TCTCCCACAACAGGATACGGAGCCTTGATTTCCATGTATTCTTGTTCAATCAGGACTTAGAATACCTGGA TGTCTCACACAATCGGTTGCAAAACATCTCTTGCTGCCCTATGGCGAGCCTGAGGCATCTAGACCTCTCA TTCAATGACTTTGATGTACTGCCTGTGTGTAAGGAATTTGGCAACCTGACGAAGCTGACTTTCCTGGGAT TAAGTGCTGCAAAGTTCCGACAACTGGATCTGCTCCCAGTTGCTCACTTGCATCTAAGCTGCATTCTTCT GGACTTAGTGAGTTATCATATAAAAGGCGGGGAAACAGAAAGTCTTCAGATTCCCAATACCACCGTTCTC CATTTGGTCTTTCATCCAAATAGCTTGTTCTCTGTTCAAGTGAACATGTCTGTAAACGCTTTAGGACATT TACAACTGAGTAATATTAAATTGAATGATGAAAACTGTCAAAGGTTAATGACATTTTTATCAGAACTCAC CAGAGGTCCAACCTTATTGAATGTGACCCTCCAGCACATAGAAACAACCTGGAAGTGCTCGGTTAAACTT TTCCAATTCTTTTGGCCCCGACCGGTGGAGTACCTCAATATTTACAACTTAACGATAACTGAGAGAATCG ACAGGGAAGAATTTACTTACTCGGAGACAGCACTGAAGTCACTGATGATAGAGCACGTCAAAAACCAAGT GTTCCTCTTTTCAAAGGAGGCGCTATACTCGGTGTTTGCTGAGATGAACATCAAGATGCTCTCTATCTCA GACACCCCTTTCATCCACATGGTGTGCCCGCCATCCCCAAGCTCATTTACATTTCTGAACTTTACCCAGA ATGTTTTTACTGACAGTGTTTTTCAAGGCTGTTCCACCTTAAAGAGATTGCAGACACTTATCTTACAAAG GAATGGTTTGAAGAACTTTTTTAAAGTAGCTCTCATGACTAAGAATATGTCCTCTCTGGAAACTTTGGAT GTTAGTTTGAATTCTTTGAACTCTCATGCATATGACAGGACATGCGCCTGGGCTGAGAGCATATTGGTGT TGAATTTGTCTTCGAATATGCTTACAGGCTCTGTCTTCAGATGCTTACCTCCCAAGGTCAAGGTCCTTGA CCTTCACAACAACAGGATAATGAGCATCCCTAAAGATGTCACCCACCTGCAGGCTTTGCAGGAACTCAAT GTAGCATCCAACTCCTTAACTGACCTTCCTGGGTGTGGGGCCTTCAGCAGCCTTTCTGTGCTGGTCATCG ACCATAACTCAGTTTCCCATCCCTCTGAGGATTTCTTCCAGAGCTGTCAGAATATTAGATCCCTAACAGC GGGAAACAACCCATTCCAATGCACATGTGAGCTGAGGGACTTTGTCAAGAACATAGGCTGGGTAGCAAGA GAAGTGGTGGAGGGCTGGCCTGACTCTTACAGGTGTGACTACCCAGAAAGCTCTAAGGGAACTGCACTGA GGGACTTCCACATGTCTCCACTGTCCTGTGATACTGTTCTGCTGACTATCACCATCGGGGCCACTATGCT GGTGCTGGCTGTCACTGGGGCTTTCCTCTGTCTCTACTTTGACCTGCCCTGGTATGTGAGGATGCTGTGT CAGTGGACACAGACCAGGCACAGGGCCAGGCACATCCCCTTAGAGGAACTCCAGAGAAACCTCCAGTTCC ATGCTTTTGTCTCATACAGTGAGCATGATTCTGCCTGGGTGAAGAACGAATTACTACCCAACCTAGAGAA AGATGACATCCGGGTTTGCCTCCATGAGAGGAACTTTGTCCCTGGCAAGAGCATTGTGGAGAACATCATC AATTTCATTGAGAAGAGTTACAAGGCCATCTTTGTGCTGTCTCCCCACTTCATCCAGAGTGAGTGGTGCC ATTATGAACTCTATTTTGCCCATCATAATCTCTTCCATGAAGGCTCTGATAACTTAATCCTCATCTTGCT GGAACCCATTCTACAGAACAACATTCCCAGTAGATACCACAAGCTGCGGGCTCTCATGGCACAGCGGACT TACTTGGAATGGCCTACTGAGAAGGGCAAACGTGGGCTGTTTTGGGCCAACCTTAGAGCTTCATTTATTA TGAAGTTAGCCTTAGTCAATGAGGATGATGTGAAAACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210761 representing BC055366 Red=Cloning site Green=Tags(s) MVKSLWDSLCNMSQDRKPIVGSFHFVCALALIVGSMTPFSNELESMVDYSNRNLTHVPKDLPPRTKALSL SQNSISELRMPDISFLSELRVLRLSHNRIRSLDFHVFLFNQDLEYLDVSHNRLQNISCCPMASLRHLDLS FNDFDVLPVCKEFGNLTKLTFLGLSAAKFRQLDLLPVAHLHLSCILLDLVSYHIKGGETESLQIPNTTVL HLVFHPNSLFSVQVNMSVNALGHLQLSNIKLNDENCQRLMTFLSELTRGPTLLNVTLQHIETTWKCSVKL FQFFWPRPVEYLNIYNLTITERIDREEFTYSETALKSLMIEHVKNQVFLFSKEALYSVFAEMNIKMLSIS DTPFIHMVCPPSPSSFTFLNFTQNVFTDSVFQGCSTLKRLQTLILQRNGLKNFFKVALMTKNMSSLETLD VSLNSLNSHAYDRTCAWAESILVLNLSSNMLTGSVFRCLPPKVKVLDLHNNRIMSIPKDVTHLQALQELN VASNSLTDLPGCGAFSSLSVLVIDHNSVSHPSEDFFQSCQNIRSLTAGNNPFQCTCELRDFVKNIGWVAR EVVEGWPDSYRCDYPESSKGTALRDFHMSPLSCDTVLLTITIGATMLVLAVTGAFLCLYFDLPWYVRMLC QWTQTRHRARHIPLEELQRNLQFHAFVSYSEHDSAWVKNELLPNLEKDDIRVCLHERNFVPGKSIVENII NFIEKSYKAIFVLSPHFIQSEWCHYELYFAHHNLFHEGSDNLILILLEPILQNNIPSRYHKLRALMAQRT YLEWPTEKGKRGLFWANLRASFIMKLALVNEDDVKT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC055366
ORF Size	2420 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC055366, AAH55366
RefSeq Size	2625 bp
RefSeq ORF	2420 bp
Locus ID	21899
Gene Summary	Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides (PubMed:19931471). In response to diacylated lipopeptides, forms the activation cluster TLR2:TLR6:CD14:CD36, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. In complex with TLR4, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion (PubMed:20037584, PubMed:23812099).[UniProtKB/Swiss-Prot Function]

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