Scyl1 (BC034519) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2810011O19Rik; C85140; mdf; mfd; Ntkl; p105
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210762 representing BC034519 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTTCTTTGCCCGGGACCCGGTCCGGGACTTCCCGTTCGAGCTGAGCCCTGAGCCCCCCGAAGGCG GGCCGCCCGGGCCCTGGATCCTGCACCGAGGCCGCAAAAAGGCCACAGGCAGCGCAGTGTCCATCTTCGT GTATGATGTGAAACCGGGAGCTGAAGAGCAGACCCAGGTGGCCAAAGCTGCCTTCAAACGCCTCAAAACT CTCCGACACCCCAACATCCTGGCCTATATCGATGGGTTGGAGACAGAAAAGTGCCTCCACATCGTGACAG AGGCTGTGACCCCCCTGGGAACATACCTCAAGGCACGAGCAGAAGCAGGTGGCCTGAAGGAGCAGGAGCT GTCATGGGGGTTACACCAGATCGTGAAAGCCCTCAGCTTCCTGGTCAACGACTGCAACCTCATCCACAAT AATGTCTGCATGGCCGCTGTGTTTGTGGACAGGGCTGGCGAGTGGAAACTTGGGGGTCTGGACTACATGT ACTCGGCACAGGGCAACGGCGGGGGACCACCCAGCAAGGGGATCCCGGAGCTCGAGCAGTATGATCCCCC GGAGCTGGCTGACAGCAGTAGCAGAGCAGTCAGAGAGAAGTGGTCAGCAGACATGTGGCGCTTGGGCTGC CTCATCTGGGAAGTTTTCAATGGGTCTCTACCTCGGGCAGCTGCCCTGCGCAACCCTGGGAAGATCCCCA AATCCCTGGTGACCCATTACTGTGAACTGGTGGGAGCTAACCCAAAAGTACGTCCCAACCCGGCCCGCTT CCTGCAGAACTGCCGGGCACCCGGTGGCTTCATGAGCAACCGCTTTGTTGAGACCAACCTCTTCCTGGAG GAGATTCAGATCAAAGAGCCAGCTGAGAAGCAGAAGTTCTTCCAAGAGCTGAGCAAGAGTCTAGACTCAT TTCCCGAAGATTTCTGTCGACACAAGGTGCTGCCCCAGCTACTGACTGCCTTTGAGTTTGGCAATGCTGG GGCCGTGGTCCTCACACCTCTCTTCAAGGTGGGAAAATCCCTCCGTGCTGAAGAGTACCAGGAGAAGATC ATCCCTGTGGTAGTTAAGATGTTCTCATCCACCGACCGGGCCATGCGCATCCGCCTCCTCCAGCAGATGG AGCAGTTCATCCAATACCTTGATGAGCCAACAGTCAACACGCAGATTTTCCCCCACGTCACACATGGCTT CCTGGACACCAACCCCGCCATCCGCGAGCAGACGGTCAAGTCCATGCTGCTCTTGGCCCCAAAGCTGAAT GAGGCCAATCTCAATGTGGAACTGATGAAGCACTTTGCAAGGCTACAAGCCAAGGACGACCAGGGTCCTA TCCGCTGCAACACCACGGTCTGCTTGGGCAAAATCGGCTCCTATCTCAGTGCTAGTACTAGACACAGGGT CCTCACCTCCGCCTTCAGCAGAGCCACTAAGGACCCATTTGCACCATCCCGGGTTGCGGGTGTCCTGGGC TTTGCTGCCACACACAATCTCTATTCGATGGACGACTGTGCCCATAAGATCCTGCCTGTGCTCTGTGGCC TTACTGTGGACCCTGAGAAATCTGTGCGGGACCAGGCCTTTAAGACCATTCGAAGCTTCCTGTCCAAATT AGAGTCTGTGTCAGAGGATCCCACCCAGCTGGCAGAAGTAGAGAAGGATGTCCATGCAGCGTCCAGTCCT GGAACAGGAGGAGCTGCAGCCAGCTGGGCAGGCTGGGCTGTGACTGGGGTATCCTCTCTCACCTCCAAGC TGATCCGAGCACACCCCACGCCTGTGCCGTCTGATACCACTGTGCCCCAGAGACCAGTGCCAGAGGGAAA TCCTGCTCCAGCCCCTGCCCTTGCCCAAGCTATCCCTGCAACCTCAGGGCACTGGGAGACACAGGAAGAC AAGGACACTGCAGAAGACAGCGCCACTGCTGACAGATGGGACGATGAGGACTGGGGCAGCTTGGAGCAGG AAGCTGAATCCGTGTTGGCACAGCAGGATGACTGGAGTGCCAAGGGCCAAGGAAGCCGAGCTGGACAGGT CAACCACCCAGACCACAAATCTCTGGAATCACATTGGAGCAGCTGGGAAGTTGAGGGCTCCTGGGACCAG GGCTGGCAGGAACCCAGCTCTGTGGAGCCACCTCCAGAAGGCACTCGGCTAGCTAGCGAATATAACTGGG GTGGTGCAGAGCCCAGTGACAAGGGCGACCCCTTTGCTGCCCTGTCTGTTCGTCCCAGCGCTCAGCCCAG GCCAGACCCAGACTCCTGGGGTGAAGACAACTGGGAAGGCTTGGAGGCTGAGAGCAGACAGGTAAAGGCA GAGCTGGCCCGGAAAAAGCGAGAGGAAAGGAGAAGAGAAATGGAAGCCAAACGGGCAGAGAAAAAGACCA CCAAGGGGCCCATGAAGCTGGGAGCCCGGAAGCTGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210762 representing BC034519 Red=Cloning site Green=Tags(s) MWFFARDPVRDFPFELSPEPPEGGPPGPWILHRGRKKATGSAVSIFVYDVKPGAEEQTQVAKAAFKRLKT LRHPNILAYIDGLETEKCLHIVTEAVTPLGTYLKARAEAGGLKEQELSWGLHQIVKALSFLVNDCNLIHN NVCMAAVFVDRAGEWKLGGLDYMYSAQGNGGGPPSKGIPELEQYDPPELADSSSRAVREKWSADMWRLGC LIWEVFNGSLPRAAALRNPGKIPKSLVTHYCELVGANPKVRPNPARFLQNCRAPGGFMSNRFVETNLFLE EIQIKEPAEKQKFFQELSKSLDSFPEDFCRHKVLPQLLTAFEFGNAGAVVLTPLFKVGKSLRAEEYQEKI IPVVVKMFSSTDRAMRIRLLQQMEQFIQYLDEPTVNTQIFPHVTHGFLDTNPAIREQTVKSMLLLAPKLN EANLNVELMKHFARLQAKDDQGPIRCNTTVCLGKIGSYLSASTRHRVLTSAFSRATKDPFAPSRVAGVLG FAATHNLYSMDDCAHKILPVLCGLTVDPEKSVRDQAFKTIRSFLSKLESVSEDPTQLAEVEKDVHAASSP GTGGAAASWAGWAVTGVSSLTSKLIRAHPTPVPSDTTVPQRPVPEGNPAPAPALAQAIPATSGHWETQED KDTAEDSATADRWDDEDWGSLEQEAESVLAQQDDWSAKGQGSRAGQVNHPDHKSLESHWSSWEVEGSWDQ GWQEPSSVEPPPEGTRLASEYNWGGAEPSDKGDPFAALSVRPSAQPRPDPDSWGEDNWEGLEAESRQVKA ELARKKREERRREMEAKRAEKKTTKGPMKLGARKLD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC034519
ORF Size	2418 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC034519, AAH34519
RefSeq Size	2648 bp
RefSeq ORF	2420 bp
Locus ID	78891
Gene Summary	Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. Involved in the maintenance of the Golgi apparatus morphology. Has no detectable kinase activity in vitro.[UniProtKB/Swiss-Prot Function]

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