Ddx42 (NM_028074) Mouse Tagged ORF Clone
CAT#: MG210774
- TrueORF®
Ddx42 (tGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (Ddx42)
ORF Plasmid: DDK
"NM_028074" in other vectors (3)
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CNY 7,890.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1810047H21Rik; AW319508; AW556242; B430002H05Rik; RHELP; RNAHP; SF3b125 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210774 representing NM_028074
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTGGAATAAAGGCGGCCCTGGAACCAAGCGGGGATTTGGTTTTGGAGGTTTTGCTATCAGTGCTG GGAAGAAGGAGGAAGCCAAACTGCCACAGCAGTCCCATAGTGCCTTTGGGGCAGCCAGCTCTTCTTCTGG ATTTGGAAAGTCTGCTCCACCACAGCTTCCTTCTTTCTACAAAATTGGATCTAAACGGGCCAACTTTGAT GAAGAAAATGCGTATTTTGAAGATGAAGAAGAAGACTCCAGCAATGTAGATTTACCTTACATTCCTGCTG AAAACTCACCTACCCGCCAGCAGTTCCATTCCAAGCCCGCAGACTCTGACAGTGATGATGACCCCTTAGA GGCATTCATGGCTGAAGTGGAGGATCAGGCTGCTAGAGACATGAAGAGACTTGAAGAAAAGGACAAGGAA AGAAAAAACGTCAAGGGTATTCGAGATGACATTGAAGAGGAAGATGACCAAGAAGCTTATTTTCGATACA TGGCAGAGAACCCAACTGCTGGGGTGGTTCAGGAGGAAGAAGAGGACAATTTGGAGTATGACAGTGATGG AAATCCAATTGCACCTTCCAAAAAAATTATCGATCCCCTTCCTCCCATTGATCATTCGGAGATTGACTAT CCACCATTTGAAAAAAATTTTTACAATGAACATGAGGAGATAACCAACCTCACTCCACAGCAGTTGATAG ACCTGCGACATAAGCTCAACCTTCGGGTCTCTGGTGCTGCACCTCCCAGACCAGGAAGTAGCTTTGCTCA TTTTGGGTTTGATGAACAACTTATGCACCAGATTAGGAAATCTGAGTACACACAGCCGACCCCAATACAG TGTCAGGGTGTACCTGTAGCACTAAGTGGGAGAGACATGATTGGTATTGCTAAAACAGGCAGTGGGAAAA CTGCAGCCTTTATTTGGCCCATGTTGATTCATATAATGGATCAGAAGGAATTGGAACCAGGTGATGGACC AATTGCAGTTATTGTGTGTCCTACTAGGGAGCTTTGTCAACAGATCCACGCAGAGTGTAAGCGGTTTGGG AAAGCGTATAATCTTCGATCAGTGGCCGTGTATGGAGGAGGGAGCATGTGGGAGCAGGCCAAGGCCCTTC AGGAAGGGGCAGAGATTGTTGTATGTACCCCAGGTCGACTGATTGATCATGTGAAGAAAAAAGCTACTAA TCTTCAAAGAGTCTCTTACCTTGTGTTTGATGAAGCCGATCGAATGTTTGACATGGGATTTGAGTATCAG GTTCGGTCCATAGCAAGTCATGTTCGTCCTGACAGACAAACTCTCTTATTCAGTGCAACTTTTCGAAAGA AGATTGAAAAGTTGGCCAGAGACATCTTGATTGACCCTATTCGAGTGGTGCAGGGAGACATTGGAGAGGC GAATGAAGATGTGACACAGATTGTGGAGATTCTCCATTCCGGACCCAGTAAATGGAACTGGCTGACTCGG CGTCTGGTGGAGTTTACTTCCTCGGGGAGTGTGCTCTTGTTTGTTACTAAGAAAGCCAATGCTGAAGAGC TAGCCAGTAACCTGAAGCAGGAGGGTCATAATCTTGGTCTGCTCCATGGGGACATGGATCAGAGCGAAAG AAACAAGGTTATTTCAGATTTTAAGAAAAAGGACATCCCCGTCCTGGTGGCCACTGATGTTGCAGCCCGT GGTCTGGATATTCCTTCCATTAAGACAGTCATTAACTATGATGTAGCACGGGACATTGATACGCACACTC ACAGGATTGGCCGGACAGGAAGAGCTGGTGAGAAGGGTGTAGCCTATACCTTATTGACTCCAAAGGACAG CAATTTTGCTGGTGACCTTGTACGGAACCTGGAAGGAGCCAACCAACATGTTTCTAAGGAGCTCTTAGAT CTTGCAATGCAGAATGCCTGGTTTCGGAAATCTCGCTTCAAAGGAGGGAAAGGCAAAAAGCTGAACATTG GTGGAGGAGGCTTGGGCTATAGAGAGCGGCCTGGGCTGGGCTCTGAGAATTCGGACCGAGGAAATAATAA CAATGTAATGAGCAATTATGAAGCCTACAAGCCCTCCACAGGAGCCATGGGAGATAGGCTCACAGCAATG AAAGCAGCTTTTCAGTCCCAGTACAAGAGTCACTTTGTTGCAGCCAGTTTAAGTAATCAGAAGGCCGGAA CCTCTTCTGCAGGGGCAAGTGGATGGACTAGTGCAGGAAGCTTAAATTCTGTTCCAACTAATTCAGCACA GCAGGGCCATAATAGTCCTGACAACCCCATGACCAGTTCTACCAAGAACATCCCAGGCTTCAACAACTCT GGGAATATCAGTAGTGCCCCAGTGACCTACCCTTCTATTGGAGCCCAAGGAGTCAACAACACAGCTTCAG GGAATAACAGCCGTGAAGGGATTGGGGGTGGCAATGGGAAGAGAGAGAGATATACTGAGAACCGGGGTGG TAGCCGTCATAGCCATGGAGATGGTGGCAATCGGCATGGAGATGGTGGGCGCCATGGAGATGGATACCGT TATCCAGAAAGTGGTAGCCGTCATACTGATGGCCACCGACATGGAGAGACCAGGCATGGAGGAAGTGCCG GCAGACACGGAGAGAGCCGGGGTGCAAATGATGGCAGGAATGGAGAAAGCAGGAAAGAAGGTTTTAATCG TGAAAACAAGATGGACCCCAAGGTGGATAGCAGCAGGATGGACAAAGTGGACAGCAAGACAGATAAGACA CCTGATGGCTTTGCTGTTCCAGAGCCACCCAAAAGAAAGAAAAGCCGATGGGATAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210774 representing NM_028074
Red=Cloning site Green=Tags(s) MNWNKGGPGTKRGFGFGGFAISAGKKEEAKLPQQSHSAFGAASSSSGFGKSAPPQLPSFYKIGSKRANFD EENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPADSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKE RKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPSKKIIDPLPPIDHSEIDY PPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQ CQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFG KAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQ VRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTR RLVEFTSSGSVLLFVTKKANAEELASNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAAR GLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLD LAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENSDRGNNNNVMSNYEAYKPSTGAMGDRLTAM KAAFQSQYKSHFVAASLSNQKAGTSSAGASGWTSAGSLNSVPTNSAQQGHNSPDNPMTSSTKNIPGFNNS GNISSAPVTYPSIGAQGVNNTASGNNSREGIGGGNGKRERYTENRGGSRHSHGDGGNRHGDGGRHGDGYR YPESGSRHTDGHRHGETRHGGSAGRHGESRGANDGRNGESRKEGFNRENKMDPKVDSSRMDKVDSKTDKT PDGFAVPEPPKRKKSRWDS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028074 |
ORF Size | 2787 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028074.4, NP_082350.3 |
RefSeq Size | 4028 bp |
RefSeq ORF | 2790 bp |
Locus ID | 72047 |
UniProt ID | Q810A7 |
Gene Summary | ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR210774 | Ddx42 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (Ddx42) |
CNY 6,808.00 |
|
MR210774L3 | Lenti ORF clone of Ddx42 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (Ddx42) |
CNY 9,030.00 |
|
MR210774L4 | Lenti ORF clone of Ddx42 (mGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (Ddx42) |
CNY 9,030.00 |