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Lrrc8a (BC048152) Mouse Tagged ORF Clone
CAT＃: MG210777
- TrueORF®
Lrrc8a (tGFP-tagged) - Mouse leucine rich repeat containing 8A (cDNA clone MGC:61242 IMAGE:5708850)
ORF Plasmid: DDK
"BC048152" in other vectors (4)
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CNY 8,930.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | mKIAA1437, MGC61242 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210777 representing BC048152
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCCGGTGACAGAGCTCCGCTACTTTGCGGACACACAGCCAGCATACCGGATCCTGAAGCCTTGGT GGGATGTGTTCACTGATTACATCTCCATCGTCATGCTGATGATTGCTGTCTTTGGAGGGACGCTGCAAGT CACCCAGGACAAGATGATCTGCCTACCTTGTAAGTGGGTCACCAAAGACTCCTGCAACGACTCCTTCCGG GGCTGGGCAGCCTCCAGCCCAGAGCCCACTTACCCCAACTCCACAGTCCTGCCGACGCCTGACACAGGCC CCACAGGTATCAAGTATGACCTAGATCGACACCAGTACAACTACGTGGATGCGGTGTGCTACGAGAACCG CCTGCATTGGTTTGCCAAGTACTTCCCCTACCTCGTGCTTCTGCACACCCTCATCTTCCTGGCCTGTAGC AACTTCTGGTTCAAGTTTCCACGCACCAGTTCGAAGCTGGAACACTTTGTGTCTATCCTGCTCAAGTGCT TCGACTCACCGTGGACCACACGGGCCCTGTCGGAGACAGTGGTGGAGGAGAGTGACCCCAAGCCAGCCTT CAGCAAGATGAACGGGTCCATGGACAAGAAGTCATCTACAGTCAGCGAGGATGTGGAGGCCACCGTGCCC ATGCTGCAGCGGACCAAGTCACGGATTGAGCAGGGCATCGTGGACCGATCGGAGACGGGCGTACTGGACA AGAAGGAAGGGGAGCAGGCGAAGGCCCTGTTTGAGAAGGTGAAGAAGTTCCGGACTCACGTGGAGGAGGG GGACATTGTGTACCGCCTGTACATGCGGCAGACCATCATCAAGGTGATCAAGTTCGCCCTCATCATCTGC TACACCGTCTACTATGTGCACAACATCAAGTTCGACGTGGACTGCACCGTGGACATCGAGAGCCTGACAG GCTACCGCACCTACCGCTGTGCCCACCCCCTGGCCACGCTCTTCAAGATCTTGGCATCCTTCTACATCAG CTTGGTCATCTTCTATGGCCTCATCTGCATGTACACACTGTGGTGGATGCTGCGGCGCTCCCTCAAGAAG TACTCGTTCGAGTCGATCCGAGAGGAGAGCAGCTACAGTGACATCCCCGACGTCAAGAACGACTTTGCCT TCATGTTGCACCTCATCGACCAGTATGACCCTCTCTACTCAAAGCGCTTCGCCGTCTTCCTGTCTGAGGT GAGTGAAAACAAGCTGCGCCAGCTGAACCTCAACAACGAGTGGACGCTGGACAAGCTGCGCCAGCGCCTC ACCAAGAACGCCCAGGACAAGCTGGAGCTGCACCTGTTCATGCTCAGCGGCATCCCGGACACTGTGTTTG ACCTGGTTGAGCTCGAGGTCCTGAAGCTGGAGCTGATACCCGACGTGACCATCCCGCCCAGCATCGCCCA GCTCACAGGCCTCAAGGAGCTGTGGCTGTACCACACGGCAGCCAAGATCGAGGCTCCTGCCCTGGCCTTC CTGCGGGAGAACCTGCGGGCCCTGCACATCAAGTTCACGGACATCAAGGAGATCCCGCTGTGGATCTACA GCCTGAAGACGCTGGAGGAGCTGCACCTGACGGGCAACCTGAGTGCAGAGAACAACCGCTACATCGTCAT TGACGGGCTGCGGGAGCTCAAACGCCTCAAGGTGCTGCGACTCAAGAGCAACCTGAGCAAGCTGCCGCAG GTGGTCACGGATGTGGGCGTGCACCTGCAGAAGCTGTCCATCAACAATGAGGGCACCAAGCTCATTGTCC TCAACAGCCTCAAGAAGATGGTTAACCTGACGGAGCTAGAGCTGATCCGCTGTGACCTGGAGCGCATCCC TCACTCCATCTTTAGCCTCCACAACCTGCAGGAGATTGACCTGAAGGACAACAACCTCAAGACCATTGAG GAGATCATCAGCTTCCAGCACCTGCACCGCCTCACCTGCCTTAAGCTGTGGTACAACCACATTGCCTACA TCCCCATCCAGATCGGCAACCTCACCAACCTCGAGCGCCTCTACCTGAACCGCAACAAAATCGAGAAAAT CCCCACCCAGCTCTTCTACTGCCGCAAGCTGCGCTACCTGGACCTCAGCCACAACAACCTGACCTTCCTC CCCGCCGACATTGGCCTCCTGCAGAACCTCCAGAACCTGGCCGTCACAGCCAATAGGATTGAAGCGCTCC CCCCAGAGCTCTTCCAGTGTCGGAAGCTACGGGCTCTACACCTGGGCAACAATGTACTGCAGTCGCTGCC CTCGAGAGTGGGCGAGCTGACCAACCTGACGCAGATCGAGCTTCGGGGCAACCGGCTGGAATGCCTGCCC GTGGAGCTGGGAGAGTGCCCCCTACTCAAGCGCAGCGGCCTGGTGGTGGAAGAGGACTTGTTCAGCACAC TGCCACCTGAGGTGAAGGAGCGGCTCTGGAGAGCTGACAAGGAGCAGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210777 representing BC048152
Red=Cloning site Green=Tags(s) MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFR GWAASSPEPTYPNSTVLPTPDTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACS NFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVP MLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFALIIC YTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKK YSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRL TKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAF LRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLSKLPQ VVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIE EIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFL PADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLP VELGECPLLKRSGLVVEEDLFSTLPPEVKERLWRADKEQA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC048152 |
| ORF Size | 2430 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC048152 |
| RefSeq Size | 4344 bp |
| RefSeq ORF | 2432 bp |
| Locus ID | 241296 |
| Gene Summary | Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:29769723). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (By similarity). Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (By similarity). Required for channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition. Can form functional channels by itself (in vitro) (By similarity). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (PubMed:14660746, PubMed:24752297). Also required for T-cell development (PubMed:24752297).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220924 | Lrrc8a (untagged) - Mouse leucine rich repeat containing 8A (cDNA clone MGC:61242 IMAGE:5708850), (10ug) |
CNY 9,120.00 |
|
| MR210777 | Lrrc8a (Myc-DDK-tagged) - Mouse leucine rich repeat containing 8A (cDNA clone MGC:61242 IMAGE:5708850) |
CNY 8,080.00 |
|
| MR210777L3 | Lenti ORF clone of Lrrc8a (Myc-DDK-tagged) - Mouse leucine rich repeat containing 8A (cDNA clone MGC:61242 IMAGE:5708850) |
CNY 9,980.00 |
|
| MR210777L4 | Lenti ORF clone of Lrrc8a (mGFP-tagged) - Mouse leucine rich repeat containing 8A (cDNA clone MGC:61242 IMAGE:5708850) |
CNY 9,980.00 |
