Cd177 (BC027283) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Pdp 3, Pdp3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210799 representing BC027283 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCTATACCAGTGCTGACCCTTCTGGGGGTCACGGCCTTGCTACCCTGTGTCCCAGCTCTGACCT GCCAGAAAAGCAGCGCACAGGCTGTGAGGAATGTGGCAGAGCTGCCCCTCAGGTGGTGGGGAGCTGGTGA GAAAACCTGCGAGGTTAGCGAGGGTTGCCAAGACTTGATAATGCTCCTGTATAATGGACCCAAGGTCAAC TTGGTGATCATCAAGGGCTGCACCGAGGTTGAGGACCAAGAGCCGAAGGTGATCTGGCTCAGGACAGGCC CTGGGCTCTCTGTGGTGTCCTACACCCGTGTGTGTCGCCATGGTGACCTCTGCAATGATGTGAACAGCAC TAAGATCCTTGAGGAGCTACCTACACCCACAGTTCCAGGGTCCCTGCGCTGCCCACTCTGCCTTTCTAAT GACAGCTGTGAGAATGCACCGGAGCAGGTCTGCCCTGTGGGAAGCACACACTGCTACGATGGAGTCCTCA GGCTCAGGGGAGATGGCATCAGGACCAATCTCAAGGTGCAGGGCTGCATGGCCCAGCCAGACTGCAACCT GCTTAATGGCACCCAGGCGATTGGGACCTTGTATATGAGCGAAAACTGTGATCTTATAGGTCCACAGGCT CTGGATTGCAATAGTGGGAGCTTGGAAACTGTGAGGAATGTATCAGATCTGCACTTGAGCTGGACGACTG GCTGGCAAACCTGTGAGGCTGGCGAGGGGTGTTATGAAACAGTGATGCTAATACAAAATGGACATGAATT TCACATGGTTCTCACTAAGGGATGTACTAGGGATATGAACAAAAAGGCTCGGCTCACCAGGCATAGAACA GGCCCAGGGATCTCCATCGTCTCCTACGTGCATGTGTGCCGCGACAGGGACTTCTGTAATGACCTGTCTA CAACAGACCCTCTTTGGACCCCGCCCCCTGACACAGAGCTAGGGACCCTGCGCTGCCGACACTGCCTTTC AACCGGCAGCTGTGTGAGTGCATCCGAGCTGGTCTGCCCCGCAGGCAGCACACACTGCTACAGTGGAGTC CTCAGCCTCAGGGGAGGAGGGGTCATTTCTGATCTGAAGGTACAGGGATGCATATCGCAGTCCCAGCCAG GATGCAACCTGCTCAACGGTACCCAGACAATCGGACCCGTGGATGTGCGTGAGGACTGCGGTCTTCAGTT AGATGCTCTGAAATGCCAGCATGGGACGCTGAAGACCATCCAGGATATATCGAAGCTGCCTCTCCAGTGG ACGGCTGGCCAGAAAATCTGTAATGTGGGTGAAGGCTGCCAAGACACACTGATGTTGATAGAGAACGGAG AGCAGGTGAACTTGGTTCTCACGAAAGGCTGCACTACCGCAAAGGACCAAGAGGCCAAAGTCACGGAGCA CAGAACTGGACCAGGGCTGTCTGTCACCTCCTACACCCGAGTGTGCCGTAAAAAAGACTTCTGCAATGAC CTGTCTACCACAGCCCCTCTCTGGGCTCCACCTCCAGTGACAGCCCCAGGGACCACTCGCTGCCCTCTCT GCTTTTCTGAACAAGCCTGTGAGAATGCACCGGAGCAGGTCTGCCCTGCAGGCAGCACACACTGCTACAG TGGAGTCCTCAGCCTCAGGGGAGGAGGGATCATCTCTGATCTGAAGGTGCAGGGCTGTATGTCCCAGCCA GGATGCAACCTGCTCAACGGTACCCAGACAATCGGACCCGTGGATGTGAGCGAGCGCTGCAGTCCTCCGT CAGAAACAACAGAGTTGTCCTGTTACAGGGGTGTGATGTTTGAGCTTGGCAATGGCTTTGCGGAGGAACC TGTCAAGTGGACGGCACCAGGGTCTCAGGTGTGTGCACCTGATGAGATTTGTCAAGAGACGCTGCTGCTC ATAGACGTAGGACAAAAATCAGCCTTCCTGGGGAGTAAAGGCTGCAGCAGTCCTGGGGCGCAGGACAATA TTGGTGTCTCCATATTCTCCCGGCTCCCTGGGATGCTGGTAGCTTCCTATACCAAATTCTGTTCTTCCCA CCTGTGCAATGGAGCCGACAGCAGCAGTGTCCTTCTAAGCATCCTCCCTCGTCCAGATGTTCCTCCCCCA GGAGATGTGCAGTGCCCCATGTGTGTAGAGTTATTTGGATCCTGCAAGAGCACTGACTCTGTCACCTGCC CTAGGGGTGCCACTCACTGTTATAAAGGTGACATTGCACTACAGGGAGGTGGACTGACTACCAGAGTGAG CATTCAGGGGTGCATGGCCCCACCTATCAAACCTTTACTGGGTGACTCCAAAACAATCGGTATCTTCTCG GCAGAGGAGAGCTCTAACTATCGACATGAGGATGATGTTACCTCGGCCCCTTCCCTGGCCTGGACCTTAC GGCTATCGGCCTGGATGTTAGGGCTATCGGCTCTTCTCAGCTCTTTGTATGCTGGGATCTGTCCTCTCTG C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210799 representing BC027283 Red=Cloning site Green=Tags(s) MNSIPVLTLLGVTALLPCVPALTCQKSSAQAVRNVAELPLRWWGAGEKTCEVSEGCQDLIMLLYNGPKVN LVIIKGCTEVEDQEPKVIWLRTGPGLSVVSYTRVCRHGDLCNDVNSTKILEELPTPTVPGSLRCPLCLSN DSCENAPEQVCPVGSTHCYDGVLRLRGDGIRTNLKVQGCMAQPDCNLLNGTQAIGTLYMSENCDLIGPQA LDCNSGSLETVRNVSDLHLSWTTGWQTCEAGEGCYETVMLIQNGHEFHMVLTKGCTRDMNKKARLTRHRT GPGISIVSYVHVCRDRDFCNDLSTTDPLWTPPPDTELGTLRCRHCLSTGSCVSASELVCPAGSTHCYSGV LSLRGGGVISDLKVQGCISQSQPGCNLLNGTQTIGPVDVREDCGLQLDALKCQHGTLKTIQDISKLPLQW TAGQKICNVGEGCQDTLMLIENGEQVNLVLTKGCTTAKDQEAKVTEHRTGPGLSVTSYTRVCRKKDFCND LSTTAPLWAPPPVTAPGTTRCPLCFSEQACENAPEQVCPAGSTHCYSGVLSLRGGGIISDLKVQGCMSQP GCNLLNGTQTIGPVDVSERCSPPSETTELSCYRGVMFELGNGFAEEPVKWTAPGSQVCAPDEICQETLLL IDVGQKSAFLGSKGCSSPGAQDNIGVSIFSRLPGMLVASYTKFCSSHLCNGADSSSVLLSILPRPDVPPP GDVQCPMCVELFGSCKSTDSVTCPRGATHCYKGDIALQGGGLTTRVSIQGCMAPPIKPLLGDSKTIGIFS AEESSNYRHEDDVTSAPSLAWTLRLSAWMLGLSALLSSLYAGICPLC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC027283
ORF Size	2451 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027283, AAH27283
RefSeq Size	2733 bp
RefSeq ORF	2453 bp
Locus ID	68891
Gene Summary	In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (By similarity). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (By similarity). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (By similarity). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:25359465). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (By similarity). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (By similarity).[UniProtKB/Swiss-Prot Function]

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