Cd177 (BC027283) Mouse Tagged ORF Clone
CAT#: MG210799
- TrueORF®
Cd177 (tGFP-tagged) - Mouse CD177 antigen (cDNA clone MGC:27738 IMAGE:2648499)
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CNY 7,980.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Pdp 3, Pdp3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210799 representing BC027283
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCTATACCAGTGCTGACCCTTCTGGGGGTCACGGCCTTGCTACCCTGTGTCCCAGCTCTGACCT GCCAGAAAAGCAGCGCACAGGCTGTGAGGAATGTGGCAGAGCTGCCCCTCAGGTGGTGGGGAGCTGGTGA GAAAACCTGCGAGGTTAGCGAGGGTTGCCAAGACTTGATAATGCTCCTGTATAATGGACCCAAGGTCAAC TTGGTGATCATCAAGGGCTGCACCGAGGTTGAGGACCAAGAGCCGAAGGTGATCTGGCTCAGGACAGGCC CTGGGCTCTCTGTGGTGTCCTACACCCGTGTGTGTCGCCATGGTGACCTCTGCAATGATGTGAACAGCAC TAAGATCCTTGAGGAGCTACCTACACCCACAGTTCCAGGGTCCCTGCGCTGCCCACTCTGCCTTTCTAAT GACAGCTGTGAGAATGCACCGGAGCAGGTCTGCCCTGTGGGAAGCACACACTGCTACGATGGAGTCCTCA GGCTCAGGGGAGATGGCATCAGGACCAATCTCAAGGTGCAGGGCTGCATGGCCCAGCCAGACTGCAACCT GCTTAATGGCACCCAGGCGATTGGGACCTTGTATATGAGCGAAAACTGTGATCTTATAGGTCCACAGGCT CTGGATTGCAATAGTGGGAGCTTGGAAACTGTGAGGAATGTATCAGATCTGCACTTGAGCTGGACGACTG GCTGGCAAACCTGTGAGGCTGGCGAGGGGTGTTATGAAACAGTGATGCTAATACAAAATGGACATGAATT TCACATGGTTCTCACTAAGGGATGTACTAGGGATATGAACAAAAAGGCTCGGCTCACCAGGCATAGAACA GGCCCAGGGATCTCCATCGTCTCCTACGTGCATGTGTGCCGCGACAGGGACTTCTGTAATGACCTGTCTA CAACAGACCCTCTTTGGACCCCGCCCCCTGACACAGAGCTAGGGACCCTGCGCTGCCGACACTGCCTTTC AACCGGCAGCTGTGTGAGTGCATCCGAGCTGGTCTGCCCCGCAGGCAGCACACACTGCTACAGTGGAGTC CTCAGCCTCAGGGGAGGAGGGGTCATTTCTGATCTGAAGGTACAGGGATGCATATCGCAGTCCCAGCCAG GATGCAACCTGCTCAACGGTACCCAGACAATCGGACCCGTGGATGTGCGTGAGGACTGCGGTCTTCAGTT AGATGCTCTGAAATGCCAGCATGGGACGCTGAAGACCATCCAGGATATATCGAAGCTGCCTCTCCAGTGG ACGGCTGGCCAGAAAATCTGTAATGTGGGTGAAGGCTGCCAAGACACACTGATGTTGATAGAGAACGGAG AGCAGGTGAACTTGGTTCTCACGAAAGGCTGCACTACCGCAAAGGACCAAGAGGCCAAAGTCACGGAGCA CAGAACTGGACCAGGGCTGTCTGTCACCTCCTACACCCGAGTGTGCCGTAAAAAAGACTTCTGCAATGAC CTGTCTACCACAGCCCCTCTCTGGGCTCCACCTCCAGTGACAGCCCCAGGGACCACTCGCTGCCCTCTCT GCTTTTCTGAACAAGCCTGTGAGAATGCACCGGAGCAGGTCTGCCCTGCAGGCAGCACACACTGCTACAG TGGAGTCCTCAGCCTCAGGGGAGGAGGGATCATCTCTGATCTGAAGGTGCAGGGCTGTATGTCCCAGCCA GGATGCAACCTGCTCAACGGTACCCAGACAATCGGACCCGTGGATGTGAGCGAGCGCTGCAGTCCTCCGT CAGAAACAACAGAGTTGTCCTGTTACAGGGGTGTGATGTTTGAGCTTGGCAATGGCTTTGCGGAGGAACC TGTCAAGTGGACGGCACCAGGGTCTCAGGTGTGTGCACCTGATGAGATTTGTCAAGAGACGCTGCTGCTC ATAGACGTAGGACAAAAATCAGCCTTCCTGGGGAGTAAAGGCTGCAGCAGTCCTGGGGCGCAGGACAATA TTGGTGTCTCCATATTCTCCCGGCTCCCTGGGATGCTGGTAGCTTCCTATACCAAATTCTGTTCTTCCCA CCTGTGCAATGGAGCCGACAGCAGCAGTGTCCTTCTAAGCATCCTCCCTCGTCCAGATGTTCCTCCCCCA GGAGATGTGCAGTGCCCCATGTGTGTAGAGTTATTTGGATCCTGCAAGAGCACTGACTCTGTCACCTGCC CTAGGGGTGCCACTCACTGTTATAAAGGTGACATTGCACTACAGGGAGGTGGACTGACTACCAGAGTGAG CATTCAGGGGTGCATGGCCCCACCTATCAAACCTTTACTGGGTGACTCCAAAACAATCGGTATCTTCTCG GCAGAGGAGAGCTCTAACTATCGACATGAGGATGATGTTACCTCGGCCCCTTCCCTGGCCTGGACCTTAC GGCTATCGGCCTGGATGTTAGGGCTATCGGCTCTTCTCAGCTCTTTGTATGCTGGGATCTGTCCTCTCTG C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210799 representing BC027283
Red=Cloning site Green=Tags(s) MNSIPVLTLLGVTALLPCVPALTCQKSSAQAVRNVAELPLRWWGAGEKTCEVSEGCQDLIMLLYNGPKVN LVIIKGCTEVEDQEPKVIWLRTGPGLSVVSYTRVCRHGDLCNDVNSTKILEELPTPTVPGSLRCPLCLSN DSCENAPEQVCPVGSTHCYDGVLRLRGDGIRTNLKVQGCMAQPDCNLLNGTQAIGTLYMSENCDLIGPQA LDCNSGSLETVRNVSDLHLSWTTGWQTCEAGEGCYETVMLIQNGHEFHMVLTKGCTRDMNKKARLTRHRT GPGISIVSYVHVCRDRDFCNDLSTTDPLWTPPPDTELGTLRCRHCLSTGSCVSASELVCPAGSTHCYSGV LSLRGGGVISDLKVQGCISQSQPGCNLLNGTQTIGPVDVREDCGLQLDALKCQHGTLKTIQDISKLPLQW TAGQKICNVGEGCQDTLMLIENGEQVNLVLTKGCTTAKDQEAKVTEHRTGPGLSVTSYTRVCRKKDFCND LSTTAPLWAPPPVTAPGTTRCPLCFSEQACENAPEQVCPAGSTHCYSGVLSLRGGGIISDLKVQGCMSQP GCNLLNGTQTIGPVDVSERCSPPSETTELSCYRGVMFELGNGFAEEPVKWTAPGSQVCAPDEICQETLLL IDVGQKSAFLGSKGCSSPGAQDNIGVSIFSRLPGMLVASYTKFCSSHLCNGADSSSVLLSILPRPDVPPP GDVQCPMCVELFGSCKSTDSVTCPRGATHCYKGDIALQGGGLTTRVSIQGCMAPPIKPLLGDSKTIGIFS AEESSNYRHEDDVTSAPSLAWTLRLSAWMLGLSALLSSLYAGICPLC TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC027283 |
ORF Size | 2453 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC027283, AAH27283 |
RefSeq Size | 2733 bp |
RefSeq ORF | 2453 bp |
Locus ID | 68891 |
Gene Summary | In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (By similarity). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (By similarity). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (By similarity). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:25359465). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (By similarity). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (By similarity).[UniProtKB/Swiss-Prot Function] |
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