Ap1g1 (BC054535) Mouse Tagged ORF Clone

CAT#: MG210836

  • TrueORF®

Ap1g1 (tGFP-tagged) - Mouse adaptor protein complex AP-1, gamma 1 subunit (cDNA clone MGC:62316 IMAGE:5698075)



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Mouse Protein / Lysate Request

CNY 7,980.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-Ap1g1 Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AA409002; Adtg; AU041323; AW551707; D8Ertd374e
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210836 representing BC054535
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGCCCCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAAGCTGAGGAAC
GAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCCTCATTTAGAGAAGAAGACAATACATACCGGTG
TCGGAATGTGGCAAAGTTACTATATATGCACATGCTGGGCTACCCTGCTCACTTTGGACAGTTGGAGTGC
CTCAAGCTAATCGCCTCACAAAAATTCACAGACAAACGCATTGGCTATTTAGGAGCAATGCTATTATTAG
ATGAAAGACAAGATGTGCATCTTCTCATGACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAGTT
TGTGCAGGGGCTAGCACTTTGTACCCTCGGCTGCATGGGCTCCTCGGAGATGTGCAGAGATCTTGCGGGA
GAGGTAGAAAAGCTTCTGAAAACCTCCAACTCTTACTTAAGAAAAAAGGCAGCACTGTGTGCTGTTCACG
TCATCAGGAAGGTTCCTGAACTTATGGAGATGTTTTTACCAGCAACAAAAAATTTATTGAATGAGAAGAA
CCATGGTGTCCTTCACACGTCTGTAGTCCTCCTCACAGAGATGTGTGAACGGAGCCCAGACATGCTCGCA
CATTTCAGAAAGAATGAAAAGCTTGTGCCCCAATTAGTTCGTATTCTAAAGAACCTCATCATGTCCGGAT
ATTCCCCAGAACATGATGTCTCTGGTATCAGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAG
AATTTTAGGACGGAATGACGATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAAT
ACAGAGACTAGTAAAAATGTAGGAAATGCTATTCTGTATGAGACGGTTTTGACTATCATGGATATTAAAT
CAGAGAGTGGATTGCGAGTTCTAGCCATAAATATTCTTGGTCGATTCTTATTGAACAATGACAAAAATAT
TAGATATGTTGCTCTGACATCTCTGTTGAAGACTGTGCAGACAGATCATAACGCTGTACAGAGGCATAGA
AGCACAATTGTGGACTGTCTGAAAGATCTGGATGTCTCCATAAAAAGACGTGCAATGGAACTGAGTTTTG
CCCTGGTGAATGGGAATAATATCCGAGGCATGATGAAAGAATTACTTTATTTTCTGGATTCCTGTGAGCC
AGAATTTAAAGCTGATTGTGCATCTGGAATCTTCCTTGCTGCAGAAAAGTACGCACCTTCCAAACGGTGG
CATATAGATACAATTATGCGTGTCTTGACAACGGCAGGAAGTTATGTTCGCGATGATGCAGTTCCTAACT
TGATTCAGTTAATAACCAACAGTGTGGAGATGCACGCTTACACCGTCCAGCGCCTGTACAAGGCGATTCT
CGGTGACTATTCTCAACAACCCTTGGTACAAGTGGCTGCGTGGTGTATAGGTGAATATGGCGATCTTCTT
GTGTCTGGCCAGTGTGAAGAGGAAGAGCCTATTCAGGTGACAGAAGATGAAGTGCTGGATATTTTAGAAA
GTGTCCTGATCTCTAATATGTCCACCTCTGTGACAAGAGGCTATGCTCTCACTGCCATTATGAAGCTGTC
TACCCGATTCACCTGTACTGTAAACCGAATTAAGAAAGTGGTTTCCATCTATGGGAGCAGCATCGACGTA
GAGCTCCAGCAGAGGGCAGTAGAGTACAACGCACTTTTTAAGAAGTATGACCACATGAGGTCTGCCCTAC
TTGAAAGAATGCCTGTCATGGAAAAAGTGACCACAAATGGCCCTTCGGAGATCGTGCAGACAAATGGAGA
GACAGAACCAGCGCCCCTAGAGACTAAACCACCACCCTCAGGGCCACAACCCACAAGCCAGGCCAATGAT
CTATTGGATTTGTTGGGAGGAAATGACATAACACCTGTTATCCCAACTGCACCTACAAGCAAACCAGCAT
CAGCTGGTGGAGAACTTCTCGACTTGCTAGGAGACATCACCCTGACAGGTGCTCCAGCTGCTGCTCCTAC
CCCTGCCTCAGTGCCACAGATATCCCAGCCCCCCTTCTTGTTGGATGGGCTTTCCTCACAGCCTCTCTTC
AATGACATCGCTCCAGGCATCCCCTCCATCACAGCGTACAGTAAGAACGGCTTGAAGATAGAGTTCACCT
TTGAACGGTCAAACACCAACCCCAGCGTAACAGTGATAACGATACAGGCCTCTAACAGCACAGAGCTAGA
CATGACGGACTTTGTTTTCCAGGCTGCAGTACCAAAGACATTCCAGCTGCAGCTTCTGTCTCCTAGCAGC
AGCGTTGTCCCAGCCTTTAATACAGGGACCATCACACAAGTCATTAAAGTTCTGAATCCACAGAAGCAAC
AGCTGCGAATGCGGATCAAGCTCACATATAATCACAAGGGCTCGGCAATGCAAGATCTAGCAGAAGTGAA
CAATTTCCCCCCTCAGTCCTGGCAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210836 representing BC054535
Red=Cloning site Green=Tags(s)

MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLGYPAHFGQLEC
LKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAG
EVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLA
HFRKNEKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATN
TETSKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHR
STIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRW
HIDTIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLL
VSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDV
ELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPSEIVQTNGETEPAPLETKPPPSGPQPTSQAND
LLDLLGGNDITPVIPTAPTSKPASAGGELLDLLGDITLTGAPAAAPTPASVPQISQPPFLLDGLSSQPLF
NDIAPGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSS
SVVPAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC054535
ORF Size 2477 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC054535, AAH54535
RefSeq Size 3916 bp
RefSeq ORF 2477 bp
Locus ID 11765
Gene Summary Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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