Ap1g1 (BC054535) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AA409002; Adtg; AU041323; AW551707; D8Ertd374e
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210836 representing BC054535 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCCCCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAAGCTGAGGAAC GAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCCTCATTTAGAGAAGAAGACAATACATACCGGTG TCGGAATGTGGCAAAGTTACTATATATGCACATGCTGGGCTACCCTGCTCACTTTGGACAGTTGGAGTGC CTCAAGCTAATCGCCTCACAAAAATTCACAGACAAACGCATTGGCTATTTAGGAGCAATGCTATTATTAG ATGAAAGACAAGATGTGCATCTTCTCATGACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAGTT TGTGCAGGGGCTAGCACTTTGTACCCTCGGCTGCATGGGCTCCTCGGAGATGTGCAGAGATCTTGCGGGA GAGGTAGAAAAGCTTCTGAAAACCTCCAACTCTTACTTAAGAAAAAAGGCAGCACTGTGTGCTGTTCACG TCATCAGGAAGGTTCCTGAACTTATGGAGATGTTTTTACCAGCAACAAAAAATTTATTGAATGAGAAGAA CCATGGTGTCCTTCACACGTCTGTAGTCCTCCTCACAGAGATGTGTGAACGGAGCCCAGACATGCTCGCA CATTTCAGAAAGAATGAAAAGCTTGTGCCCCAATTAGTTCGTATTCTAAAGAACCTCATCATGTCCGGAT ATTCCCCAGAACATGATGTCTCTGGTATCAGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAG AATTTTAGGACGGAATGACGATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAAT ACAGAGACTAGTAAAAATGTAGGAAATGCTATTCTGTATGAGACGGTTTTGACTATCATGGATATTAAAT CAGAGAGTGGATTGCGAGTTCTAGCCATAAATATTCTTGGTCGATTCTTATTGAACAATGACAAAAATAT TAGATATGTTGCTCTGACATCTCTGTTGAAGACTGTGCAGACAGATCATAACGCTGTACAGAGGCATAGA AGCACAATTGTGGACTGTCTGAAAGATCTGGATGTCTCCATAAAAAGACGTGCAATGGAACTGAGTTTTG CCCTGGTGAATGGGAATAATATCCGAGGCATGATGAAAGAATTACTTTATTTTCTGGATTCCTGTGAGCC AGAATTTAAAGCTGATTGTGCATCTGGAATCTTCCTTGCTGCAGAAAAGTACGCACCTTCCAAACGGTGG CATATAGATACAATTATGCGTGTCTTGACAACGGCAGGAAGTTATGTTCGCGATGATGCAGTTCCTAACT TGATTCAGTTAATAACCAACAGTGTGGAGATGCACGCTTACACCGTCCAGCGCCTGTACAAGGCGATTCT CGGTGACTATTCTCAACAACCCTTGGTACAAGTGGCTGCGTGGTGTATAGGTGAATATGGCGATCTTCTT GTGTCTGGCCAGTGTGAAGAGGAAGAGCCTATTCAGGTGACAGAAGATGAAGTGCTGGATATTTTAGAAA GTGTCCTGATCTCTAATATGTCCACCTCTGTGACAAGAGGCTATGCTCTCACTGCCATTATGAAGCTGTC TACCCGATTCACCTGTACTGTAAACCGAATTAAGAAAGTGGTTTCCATCTATGGGAGCAGCATCGACGTA GAGCTCCAGCAGAGGGCAGTAGAGTACAACGCACTTTTTAAGAAGTATGACCACATGAGGTCTGCCCTAC TTGAAAGAATGCCTGTCATGGAAAAAGTGACCACAAATGGCCCTTCGGAGATCGTGCAGACAAATGGAGA GACAGAACCAGCGCCCCTAGAGACTAAACCACCACCCTCAGGGCCACAACCCACAAGCCAGGCCAATGAT CTATTGGATTTGTTGGGAGGAAATGACATAACACCTGTTATCCCAACTGCACCTACAAGCAAACCAGCAT CAGCTGGTGGAGAACTTCTCGACTTGCTAGGAGACATCACCCTGACAGGTGCTCCAGCTGCTGCTCCTAC CCCTGCCTCAGTGCCACAGATATCCCAGCCCCCCTTCTTGTTGGATGGGCTTTCCTCACAGCCTCTCTTC AATGACATCGCTCCAGGCATCCCCTCCATCACAGCGTACAGTAAGAACGGCTTGAAGATAGAGTTCACCT TTGAACGGTCAAACACCAACCCCAGCGTAACAGTGATAACGATACAGGCCTCTAACAGCACAGAGCTAGA CATGACGGACTTTGTTTTCCAGGCTGCAGTACCAAAGACATTCCAGCTGCAGCTTCTGTCTCCTAGCAGC AGCGTTGTCCCAGCCTTTAATACAGGGACCATCACACAAGTCATTAAAGTTCTGAATCCACAGAAGCAAC AGCTGCGAATGCGGATCAAGCTCACATATAATCACAAGGGCTCGGCAATGCAAGATCTAGCAGAAGTGAA CAATTTCCCCCCTCAGTCCTGGCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210836 representing BC054535 Red=Cloning site Green=Tags(s) MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLGYPAHFGQLEC LKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAG EVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLA HFRKNEKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATN TETSKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHR STIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRW HIDTIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLL VSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDV ELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPSEIVQTNGETEPAPLETKPPPSGPQPTSQAND LLDLLGGNDITPVIPTAPTSKPASAGGELLDLLGDITLTGAPAAAPTPASVPQISQPPFLLDGLSSQPLF NDIAPGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSS SVVPAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC054535
ORF Size	2477 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC054535, AAH54535
RefSeq Size	3916 bp
RefSeq ORF	2477 bp
Locus ID	11765
Gene Summary	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.[UniProtKB/Swiss-Prot Function]

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