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Sidt1 (NM_198034) Mouse Tagged ORF Clone
CAT＃: MG210846
- TrueORF®
Sidt1 (tGFP-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1)
ORF Plasmid: DDK
"NM_198034" in other vectors (3)
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CNY 7,656.00
CNY 7,980.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW045928; B830021E24Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210846 representing NM_198034
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGACTGCCTGCGCCTGGCGCTGCTCTGCGCGCTGCCCTGGCTCCTGCGGGCGGCGGTCCCTGGCC ACCAGGAAGAGCCCCTCGCGAAATCCGCAGAGCTGCGGCGCGATCCGCGAGACCCCGCCAGGGGAGCGGA TTTCGATCGCGTCTACAGCGGGGTGGTGAGCCTCAGCACCGAGAACATCTACTCCTTCAACCACACCAGC CATCCTGGCCAGGTGACAGCCGTGCGGGTGCATGTCAACAGTTCCTCTGACAACCTGGACTACCCAGTCC TGGTTGTGGTTCGCCAGCAGAAAGAAGTGTTGTCCTGGCAGGTCCCTCTGCTCTTCCAAGGACTATACCA AAGGAGCTACAACTACCAGGAAGTCAGCCGCACTCTATGCCCTTCAAAAGCAACCAATGAGACGGGCCCT TTGGAGCAGCTGATCTTTGTAGATGTAGCGTCCATGGCGCCCCATGGTGCCCACTACAAGTTGCTGGTCA CCAAAATCAAGCACTTCCAGCTCCCGACGAATGTTGCCTTCTACTTCACTGCCAGCCCCTCTCAACCTCA GTATTTTCTATACAAATTTCCTGAAGACGTGGACTCCGTTATCATTAAAGTGGTGTCTGAGAAGGCTTAC CCGTGTTCAGTTGTCTCCGTTCAGAACATCATGTGTCCAGTGTATGATCTGGACCACAATGTGGAATTCA ATGGTGTCTATCAGTCCATGACCAAGAAAGCCGCCATCACGCTGCAGAAGAAGGATTTTCCAGATGAGCA GTTCTTCGTGGTGTTTGTGATAAAACCTGAAGATTATGCCTGTGGCGGATCATTCTCCATCCAGGAAAAT GAAAACCAGACCTGGAATCTACAACGATCAAAGAACCTGAAAGTGACCATTGTCCCATCCATCAAAGAAT CTGTATATGTGAAATCTAGTCTTTTCAGCATATTCGTTTTCTTGTCCTTCTACTTGGGATGCCTGCTCGT TGTGCTTGTCCATCATGTGAGGTTTCAGAGGAAATCTATTGACGGAAGCTTTGGTTCCAGCGATGGCTCT GGAAATATGGCTGTCTCACATCCCATCACTGCCAGCACGCCCGAAGGGAGCAATTATGGGGCAATAGATG AGTCCAGTTCCAGTCCTGGGAGGCAGATGTCTTCTTCCGATGGTGGGCAGCCTTGCCACTCAGACACGGA CAGCTCTGTGGAGGAGAGTGACTTTGATACCATGCCTGACATCGAAAGCGACAAAAACGTCATCCGGACC AAGATGTTCTTGTACCTGTCAGATCTGTCCAGGAAGGACCGGAGGATTGTCAGCAAAAAGTATAAGATTT ATTTTTGGAACATCATCACCATCGCTGTGTTTTATGCACTGCCTGTGATGCAGCTTGTTATCACCTACCA GACAGTGGTGAATGTCACTGGCAACCAGGATATCTGTTACTACAACTTCCTTTGTGCTCACCCCCTGGGT GTCCTGAGCGCCTTCAATAATATTCTCAGTAACCTGGGCCACGTACTCCTGGGCTTCCTCTTCCTGCTGA TAGTTCTGCGCAGAGACCTTCTCCACCGAAGAGCCCTGGAAGCTAAAGACATCTTTGCTATGGAATACGG TATTCCCAAACACTTTGGTCTCTTCTACGCTATGGGGATAGCACTGATGATGGAGGGCGTGCTCAGTGCT TGTTACCATGTCTGCCCTAATTACTCCAACTTCCAGTTCGACACTTCCTTCATGTACATGATCGCTGGCC TCTGCATGCTGAAGCTCTATCAGACCCGCCACCCGGACATCAACGCCAGCGCCTACTCTGCTTATGCCTC CTTCGCTGTGGTCATCACACTGACGGTCCTCGGAGTGGTGTTTGGAAAAAACGATGTGTGGTTCTGGATC ATCTTCTCTGCCATCCACATTCTCTCTTCTCTGGCCCTCAGCACCCAAATCTACTACATGGGCCGTTTCA AGATAGATCTGGGAATCTTCCGGCGGGCTGCTATGGTGTTCTACACGGACTGTATCCAGCAATGCAGCAG ACCTCTGTATATGGACAGGATGGTGTTGCTCATTGTGGGAAATCTGGTTAATTGGTCCTTTGCCTTCTTT GGATTGATCTACCGGCCCAGGGACTTTGCCTCCTACATGCTGGGCATCTTCATCTGCAACCTGCTGCTGT ACTTGGCTTTCTACATCATCATGAAGCTTCGAAGCTCTGAGAAAGTCCTCCCGCTACCAGTCTTCTGCAT CGCGGCCACTGCCGTGGTGTGGGCTGCGGCACTGTACTTCTTCTTCCAGAACCTCAGCAGCTGGGAGGGG ACCCCTGCAGAATCCCGGGAGAAGAACCGTGAGTGTGTCCTGCTGGATTTCTTTGATGACCATGACATCT GGCACTTCCTCTCTGCGACTGCCCTGTTTTTCTCATTCCTGGTGCTGTTGACCCTGGATGATGACCTGGA TGTGGTCCGGAGAGACCAGATCCCTGTCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210846 representing NM_198034
Red=Cloning site Green=Tags(s) MLDCLRLALLCALPWLLRAAVPGHQEEPLAKSAELRRDPRDPARGADFDRVYSGVVSLSTENIYSFNHTS HPGQVTAVRVHVNSSSDNLDYPVLVVVRQQKEVLSWQVPLLFQGLYQRSYNYQEVSRTLCPSKATNETGP LEQLIFVDVASMAPHGAHYKLLVTKIKHFQLPTNVAFYFTASPSQPQYFLYKFPEDVDSVIIKVVSEKAY PCSVVSVQNIMCPVYDLDHNVEFNGVYQSMTKKAAITLQKKDFPDEQFFVVFVIKPEDYACGGSFSIQEN ENQTWNLQRSKNLKVTIVPSIKESVYVKSSLFSIFVFLSFYLGCLLVVLVHHVRFQRKSIDGSFGSSDGS GNMAVSHPITASTPEGSNYGAIDESSSSPGRQMSSSDGGQPCHSDTDSSVEESDFDTMPDIESDKNVIRT KMFLYLSDLSRKDRRIVSKKYKIYFWNIITIAVFYALPVMQLVITYQTVVNVTGNQDICYYNFLCAHPLG VLSAFNNILSNLGHVLLGFLFLLIVLRRDLLHRRALEAKDIFAMEYGIPKHFGLFYAMGIALMMEGVLSA CYHVCPNYSNFQFDTSFMYMIAGLCMLKLYQTRHPDINASAYSAYASFAVVITLTVLGVVFGKNDVWFWI IFSAIHILSSLALSTQIYYMGRFKIDLGIFRRAAMVFYTDCIQQCSRPLYMDRMVLLIVGNLVNWSFAFF GLIYRPRDFASYMLGIFICNLLLYLAFYIIMKLRSSEKVLPLPVFCIAATAVVWAAALYFFFQNLSSWEG TPAESREKNRECVLLDFFDDHDIWHFLSATALFFSFLVLLTLDDDLDVVRRDQIPVF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198034 |
| ORF Size | 2481 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198034.3 |
| RefSeq Size | 4267 bp |
| RefSeq ORF | 2484 bp |
| Locus ID | 320007 |
| UniProt ID | Q6AXF6 |
| Gene Summary | In vitro binds long double-stranded RNA (dsRNA) (500 and 700 base pairs), but not dsRNA shorter than 300 bp (PubMed:26067272). Not involved in RNA autophagy, a process in which RNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Systemic RNA Interference Deficiency-1 (SID-1) extracellular domain selectively binds long double-stranded RNA and is required for RNA transport by SID-1
,Li, W;Koutmou, KS;Leahy, DJ;Li, M;,
J. Biol. Chem.
,PubMed ID 26067272
[SIDT1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210846 | Sidt1 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1), transcript variant 2 |
CNY 6,056.00 |
|
| MR210846L3 | Lenti ORF clone of Sidt1 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1), transcript variant 2 |
CNY 9,120.00 |
|
| MR210846L4 | Lenti ORF clone of Sidt1 (mGFP-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1), transcript variant 2 |
CNY 9,120.00 |
