Nfatc1 (BC061509) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Nfatcb, NFAT2, NFATc, NF-ATc
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210848 representing BC061509 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAAGTACCAGCTTTCCAGTCCCTTCCAAGTTTCCACTCGGCCCTCCGGCCGCAGTCTGCGGGAGCG GAGAAACTTTGCGGCCCGCGCCGCCCTCCGGCGGCACCATGAAGGCGGCCGAGGAAGAACACTACAGTTA TGTGTCCCCTAGTGTCACCTCGACCCTGCCCCTTCCCACAGCACACTCTGCCTTGCCAGCAGCATGCCAC GACCTCCAGACGTCCACCCCGGGTATCTCAGCTGTTCCTTCAGCCAATCATCCCCCCAGTTACGGAGGGG CTGTGGACAGCGGGCCTTCGGGATACTTCCTGTCCTCTGGCAACACCAGACCCAACGGGGCCCCGACTCT GGAGAGTCCGAGAATCGAGATCACCTCCTACCTGGGCCTACACCATGGCAGCGGCCAGTTTTTCCACGAC GTGGAGGTGGAAGACGTACTTCCTAGCTGCAAGCGCTCACCGTCTACAGCAACCCTGCACCTGCCCAGCC TGGAAGCCTACAGAGACCCCTCCTGCCTGAGCCCAGCCAGCAGTCTCTCCTCCAGAAGCTGTAACTCTGA GGCCTCCTCCTACGAGTCCAACTACTCCTACCCATACGCGTCCCCCCAGACCTCTCCGTGGCAGTCACCC TGCGTGTCTCCCAAGACCACGGACCCGGAGGAGGGTTTTCCCCGAAGCCTGGGTGCCTGCCACCTGCTAG GATCGCCCAGGCACTCCCCATCCACCTCTCCTCGGGCAAGCATCACGGAGGAGAGCTGGCTCGGTGCCCG CGGCTCCCGGCCCACGTCCCCCTGCAACAAGCGCAAGTACAGTCTCAATGGCCGGCAGCCCTCCTGCTCA CCCCACCACTCACCCACACCATCCCCCCATGGCTCCCCTCGGGTCAGTGTGACCGAAGATACCTGGCTCG GTAACACCACCCAGTATACCAGCTCTGCCATTGTGGCAGCCATCAACGCCCTGACCACCGATAGCACTCT GGACCTGGGTGATGGGGTCCCTATCAAGTCTCGAAAGACAGCACTGGAGCATGCGCCCTCTGTGGCCCTC AAAGTAGAGCCAGCTGGGGAAGACCTGGGCACCACTCCACCCACTTCTGACTTCCCACCCGAGGAGTACA CCTTCCAGCACCTTCGGAAGGGTGCCTTTTGCGAGCAGTATCTGTCGGTGCCACAGGCCTCGTATCAGTG GGCGAAGCCCAAGTCTCTTTCCCCGACATCATATATGAGCCCATCCTTGCCTGCCCTTGACTGGCAGCTC CCGTCACATTCTGGTCCATACGAGCTTCGGATCGAGGTGCAGCCCAAGTCTCACCACAGGGCTCACTATG AGACGGAAGGCAGCCGGGGGGCTGTGAAGGCTTCAGCTGGAGGACACCCCATTGTGCAGCTACACGGTTA CTTGGAGAATGAACCTCTCACGCTACAGCTGTTCATTGGGACGGCTGACGACCGCCTGCTGAGGCCCCAC GCCTTCTACCAGGTCCACCGGATCACGGGGAAGACTGTCTCCACCACCAGCCACGAGATCATCCTGTCCA ACACCAAAGTCCTGGAGATCCCGTTGCTTCCAGAAAATAACATGCGAGCCATCATCGACTGTGCTGGGAT CCTGAAGCTCAGAAACTCTGATATTGAGCTGAGGAAAGGGGAGACAGACATCGGGAGGAAGAACACCAGG GTGAGGCTGGTCTTCCGAGTTCACATCCCACAGCCCAATGGCCGGACGCTGTCTCTCCAGGTGGCCTCGA ACCCTATCGAGTGTTCCCAGCGGTCAGCCCAGGAGCTGCCCCTCGTGGAGAAGCAGAGCACAGACAGCTA CCCAGTCATCGGCGGGAAGAAGATGGTGCTGTCTGGCCATAACTTTCTGCAAGACTCCAAAGTCATTTTC GTGGAGAAGGCTCCAGATGGCCACCACGTCTGGGAGATGGAAGCAAAGACTGACCGGGACCTGTGCAAGC CAAATTCCCTGGTGGTTGAGATACCACCTTTCCGCAACCAGAGGATAACCAGCCCCGTCCAAGTCAGTTT CTATGTCTGCAACGGGAAACGGAAGAGAAGCCAGTACCAGCGTTTCACGTACCTTCCTGCCAATGTTCCA ATTATAAAGACAGAACCCACGGACGACTTTGAGCCAGCTCTGACCTGTGGACCAATGAGCCAGGGGATTA GTCCTCTGCCGAGGCCTTACTACAGCCAACAGCTCACCATGCCTCCCGACCCCGGCTCCTGCCTCGTGGC TGGCTTCGCCCCCTGCTCCCAGAGGAACACGCTGATGCCCACGCCTCCCAACGCAAGCCCGAAGCTCCAC GACCTTTCCTCTCCTGCCTACACCAAGGGCCTCACCAACCCGGGCCACAGTGGTCACCTTGGACTTCAGC CACCCGCTTCGGAGGCCCCCACCATGCAGGAAGTGCCGAGACCCATGGCCATCCAACCCAACTCGCCTGA GCAGCCCCCATCCGCCAGGCTACAGCCGCAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210848 representing BC061509 Red=Cloning site Green=Tags(s) MPSTSFPVPSKFPLGPPAAVCGSGETLRPAPPSGGTMKAAEEEHYSYVSPSVTSTLPLPTAHSALPAACH DLQTSTPGISAVPSANHPPSYGGAVDSGPSGYFLSSGNTRPNGAPTLESPRIEITSYLGLHHGSGQFFHD VEVEDVLPSCKRSPSTATLHLPSLEAYRDPSCLSPASSLSSRSCNSEASSYESNYSYPYASPQTSPWQSP CVSPKTTDPEEGFPRSLGACHLLGSPRHSPSTSPRASITEESWLGARGSRPTSPCNKRKYSLNGRQPSCS PHHSPTPSPHGSPRVSVTEDTWLGNTTQYTSSAIVAAINALTTDSTLDLGDGVPIKSRKTALEHAPSVAL KVEPAGEDLGTTPPTSDFPPEEYTFQHLRKGAFCEQYLSVPQASYQWAKPKSLSPTSYMSPSLPALDWQL PSHSGPYELRIEVQPKSHHRAHYETEGSRGAVKASAGGHPIVQLHGYLENEPLTLQLFIGTADDRLLRPH AFYQVHRITGKTVSTTSHEIILSNTKVLEIPLLPENNMRAIIDCAGILKLRNSDIELRKGETDIGRKNTR VRLVFRVHIPQPNGRTLSLQVASNPIECSQRSAQELPLVEKQSTDSYPVIGGKKMVLSGHNFLQDSKVIF VEKAPDGHHVWEMEAKTDRDLCKPNSLVVEIPPFRNQRITSPVQVSFYVCNGKRKRSQYQRFTYLPANVP IIKTEPTDDFEPALTCGPMSQGISPLPRPYYSQQLTMPPDPGSCLVAGFAPCSQRNTLMPTPPNASPKLH DLSSPAYTKGLTNPGHSGHLGLQPPASEAPTMQEVPRPMAIQPNSPEQPPSARLQPQ SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC061509
ORF Size	2483 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC061509, AAH61509
RefSeq Size	4626 bp
RefSeq ORF	2483 bp
Locus ID	18018

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.