Senp6 (BC061480) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mKIAA0797, Susp1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210918 representing BC061480 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGCAAGAGCGGTGGCAGCGCTGGGGCTCTTTTCCTGAAAGCTTTGGATAGATCAGAATCTA AGAGGGATGGAGGCTTTAAAAATAATTGGAGCTTTGATCATGAAGAAGAAAGTGAAGGAGATGCAGACAA AGATGGGGCAAATCTACTCAGTGTAGAAGATGAGGATTCTGAAATCTCAAAAGGAAAAAAGTTAAATCGG AGATCTGAAATTGTTGCTACTAGTTCTGGTGATTTCATCTTGAAGACTTATGTAAGGCGGAGCAAGACTG ACGGCTTTAAAACTTTGAAAGGCAACCCAATTGGACTTAACATGTTAAGCAACAATAAGAAACTGAGTGA AAGCACCGCAGCCACAGCATTATGTTCTGGAACTGTGGTTCATGGGAGGCGCTTCCATCATGCTCACTCA CAGACGCCAGGCATCAGAACAGCGGCTCAGAGGAAAGAATACCCACCTTATGTCCACAAAGCTGAAAACA GCCCTGTAATGCTAAGTCATGGCCAAGGTGGAGACCATATAATGAAGAAAACTGAAGAGTCAGAATCATA TGTAGAGTCTGAGATTAAGAGGAAAGTCCAGCAGAAGCGGCACTGTAGCACTTACCAGCTTTCTCCTCTG TCCCCTGCCTCAAAGAAGTGTTTGACCCATTTAGAGGTTTCTGAACAGCGTGAATATTGCCCAAAATGTG GAAAAGAAAAAGAAAATCAGACCAAATGCCAAAGTTGTGGTATTGTTTTTCATAATGATTTGCAAAGAAA TTGCCGACAAGCCGTAACTTTGAATGAACCTACTGGACCATTGCTAAGAACATCAATTCATCAGAACTCT GGAGGACAGAAATCACAAAACACAGGACTAACAGCTAAGAAATTTTATGGGAACAGTGTGGATAAGATTC CAATTGATATTTTGGTCACCTGTGATGATAGCAGACATAATTACATACAGACAAATGGGAAAGTCATTTT ACCTGGAGGAAAAATACCTAAACTCACAAACCCAAAAGAGCGAAAGATAAGCGTGTCAGATTTAAACGAT CCAATCATTTTGTCTAGTGATGATGATGACGACGACGACGATAGGACTAAGAGAAGAGAGAGCACGTCCC CGCAGCCTGCCGACTCAGCATGCTCCTCACCAGTACCATCTACGGGAAAAGTTGAAGCTGCACTAAATGC AGATGCCTGTAGAGCAGAGCAGGAACCCAGAAGCAGTCCAGCAGAGCCTGAGTTAAATACCATTGTCATA CCACGAAAAGCAAGAATGAAGGACCAGCTTGGTAATTCCATCAGCACACCTTTAAAACGTCGTAAAGTAA ACAGTCATGCAGCTTTTATTCACCCTATGTCTTTAAGTTGCCAAAACTTTGAAAGTGTCATTTTAAACTG CCGAAGTATACGAGTTGGAACACTCTTCCGACTGTTGGTAGAGCCTGTAATTTTTTCTTTAGAGTCAATC ACGATACATCTAGATGGACCAGAAAGTGATCCTGTAGACATTATTTTAAATACCTCTGATCTAACTAAAT GTGAATGGTGTAATGTCCGGAAATTACCAGTAGTGTTTCTCCAGGCAATACCAGCAGTTTACCAGAAGCT GAGCATGCAGCTACAGATGAGTAAAGAAGATAAAGTTTGGAATGATTGTAAAGGAATAAACAGGATAACA AGTTTAGAAGAACAGTACATTATTTTAATTTTTCAAACTGGCCTTGATCATCAGGCAGAGGTGGTCTTTG AAAGTATCATTACTGACATTGGTATAAGGAATAATGTTCCCAACTTTTTTGCAAAAATTCTTTTTGACGA AGCCAATAGCAGACTTGTTGCCTGTACAAGAAGCTATGAAGAGAGCATCAAAGGAAACTGCGCGCAAAAA GAGAACAAAGTGAAAACTGTGTCGTTTGAATCTAAAATACAACTTAGAAGCAAACAAGAATTGCAGTTTT TTGATGATGACGAAGAAGCTGGGGAGAGCCATACAATCTTCATTGGCCCTGTAGAAAAATTGATAGTATA CCCACCACCTCCAGCTAAGGGAGGCATTTCTGTTACTAATGAGGACCTGCACTGTCTAAGTGAAGGAGAG TTTTTAAATGATGTTATTATCGACTTTTATTTGAAATACTTGGTGCTTGAAAAACTGAAGAAAGAAGACG CTGACCGAATTCATATATTTAGTTCTTTTTTCTATAAACGCCTTAATCAGAGAGAGAGGAGAAATCCTGA CACAACTAATCTGTCGATACAACAAAAACGGCATGGGAGAGTAAAAACGTGGACACGGCATGTAGATATT TTTGAGAAGGACTTTATTTTTGTACCCCTTAATGAAGCTGCACATTGGTTTTTGGCTGTTGTTTGTTTCC CTGGTTTGGAAAAACCAAAATATGAACCTAATCCTCATTACCATGAAAATGCAGTCATGCAAAAACTCCA AGTGCAGAGGACAGTTGTGTTTCTTCTGCCAGTGAGATGGGTGCTTGTTCACAGAACTCTGCTGCCAAGC CTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210918 representing BC061480 Red=Cloning site Green=Tags(s) MAAGKSGGSAGALFLKALDRSESKRDGGFKNNWSFDHEEESEGDADKDGANLLSVEDEDSEISKGKKLNR RSEIVATSSGDFILKTYVRRSKTDGFKTLKGNPIGLNMLSNNKKLSESTAATALCSGTVVHGRRFHHAHS QTPGIRTAAQRKEYPPYVHKAENSPVMLSHGQGGDHIMKKTEESESYVESEIKRKVQQKRHCSTYQLSPL SPASKKCLTHLEVSEQREYCPKCGKEKENQTKCQSCGIVFHNDLQRNCRQAVTLNEPTGPLLRTSIHQNS GGQKSQNTGLTAKKFYGNSVDKIPIDILVTCDDSRHNYIQTNGKVILPGGKIPKLTNPKERKISVSDLND PIILSSDDDDDDDDRTKRRESTSPQPADSACSSPVPSTGKVEAALNADACRAEQEPRSSPAEPELNTIVI PRKARMKDQLGNSISTPLKRRKVNSHAAFIHPMSLSCQNFESVILNCRSIRVGTLFRLLVEPVIFSLESI TIHLDGPESDPVDIILNTSDLTKCEWCNVRKLPVVFLQAIPAVYQKLSMQLQMSKEDKVWNDCKGINRIT SLEEQYIILIFQTGLDHQAEVVFESIITDIGIRNNVPNFFAKILFDEANSRLVACTRSYEESIKGNCAQK ENKVKTVSFESKIQLRSKQELQFFDDDEEAGESHTIFIGPVEKLIVYPPPPAKGGISVTNEDLHCLSEGE FLNDVIIDFYLKYLVLEKLKKEDADRIHIFSSFFYKRLNQRERRNPDTTNLSIQQKRHGRVKTWTRHVDI FEKDFIFVPLNEAAHWFLAVVCFPGLEKPKYEPNPHYHENAVMQKLQVQRTVVFLLPVRWVLVHRTLLPS L TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC061480
ORF Size	2525 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC061480, AAH61480
RefSeq Size	4201 bp
RefSeq ORF	2525 bp
Locus ID	215351
Gene Summary	Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.[UniProtKB/Swiss-Prot Function]

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