Vav3 (BC052739) Mouse Tagged ORF Clone

CAT#: MG210938

  • TrueORF®

Vav3 (tGFP-tagged) - Mouse vav 3 oncogene (cDNA clone MGC:63440 IMAGE:6832850)



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Mouse Protein / Lysate Request

CNY 9,310.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MGC27838
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210938 representing BC052739
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCGTGGAAGCAGTGCGCTCAGTGGCTCATCCACAGCAAGGTGCTGCCCCCCAACCATCGGGTGA
CCTGGGACTCGGCGCAGGTGTTCGACCTGGCGCAGACCCTTCGCGATGGAGTCCTGCTCTGCCAGCTGCT
CAACAACCTGCGGCCACACTCCATCAATCTGAAGGAGATCAACCTGAGGCCCCAGATGTCCCAGTTTCTC
TGTTTGAAGAACATCCGGACATTCCTGGCCGCCTGCTGCGATACCTTTGGAATGAGGAAAAGTGAACTTT
TTGAGGCATTTGACCTATTTGATGTTCGAGACTTTGGAAAGGTTATAGAAACATTATCTCGGCTATCCCG
AACACCAATAGCATTGGCCACAGGAATCAGGCCGTTCCCCACTGAAGAAAGTATAAATGATGAAGACATC
TACAAAGGCCTTCCCGACTTAATAGATGAAACCCGCGTGGAAGATGAAGAAGACCTCTATGACTGTGTGT
ACGGCGAGGATGAAGGCGGAGAAGTCTATGAAGACTTGATGAAGGCGGAGGAAGCCCAGCAACCTAAATC
ACAAGAAAATGATATACGGAGTTGTTGCCTAGCAGAAATTAGGCAGACAGAAGAAAAATACACAGAAACG
TTGGAGTCAATAGAAAAATATTTCATGGCACCATTGAAAAGATTTCTGACGGCGGCGGAATTTGATTCAG
TATTTATTAACATTCCTGACCTTGTAAAAGTTCATCGGAGCTTAATGCAAGAGATTCATGATTCCATTGT
AAATAAAGATGACCAGAACTTGTATCAAGTTTTTATTAACTACAAGGAAAGATTGGTTATTTATGGGCAG
TACTGCAGTGGAGTGGAGTCAGCCATCTCTAACCTAGACTACATCTCTAAGACAAAAGAAGATGTCAAAC
TGAAATTAGAGGAATGTTCAAAAAGAGCCAATAACGGGAAGTTTACTCTGCGCGATTTGCTGGTCGTTCC
AATGCAGCGCGTGCTGAAGTATCACCTCCTCCTTCAGGAGCTGGTAAAACACACCCATGACCCTATGGAG
AAGGCGAACCTGAAGCTGGCTCTGGATGCAATGAAGGACTTGGCACAATATGTGAATGAAGTAAAAAGAG
ATAATGAGACCCTTCGTGAAATTAAGCAGTTTCAGCTATCTATAGAGAATTTGAACCAGCCAGTTTTGCT
CTTTGGACGACCACAGGGAGACGGTGAAATCCGAATAACCACTCTGGACAAGCATACAAAACAAGAAAGG
CATATCTTCTTATTCGACTTGGCAGTGATTGTCTGTAAAAGGAAAGGCGATAACTACGAGATGAAGGAAA
TAATAGATCTCCAGCAGTACAAGATAGCCAACAACCCTACCACTGACAAAGAGAATAAAAAGTGGTCTTA
TGGCTTCTACCTCATCCATACCCAAGGACAAAATGGGTTAGAATTTTATTGCAAAACAAAAGATTTAAAG
AAAAAATGGCTGGAACAGTTTGAAATGGCTCTATCCAACATAAGGCCAGACTATGCAGACTCCAATTTCC
ATGATTTCAAGATGCACACCTTCACTCGGGTGACATCCTGCAGGGTCTGCCAGATGCTCCTGAGGGGAAC
ATTTTATCAAGGCTACTTATGTTTTAAGTGTGGAGCAAAAGCACATAAAGAATGTTTGGGAAGAGTAGAC
AATTGTGGCAGAGTTAATTCCGTTGAACAAGGGCCATTCAAACCACCAGAGAAACGGACCAATGGACTTC
GGAGAGCTTCCAGACAGGTGGACCCAGGTTTGCCGAAGATGCAGGTCATTAGGAACTACACTGGCACCCC
GGCCCCAGGGCTTCATGAAGGACCACCATTGCATATCCAGGCAGGCGACACTGTTGAACTTCTGAGAGGA
GATGCACACAGTGTGTTCTGGCAGGGACGAAACTTAGCTTCAGGAGAGGTTGGATTTTTCCCAAGTGATG
CTGTCAAACCCTCTCCATGTGTACCTAAACCAGTAGATTATTCTTGCCAACCCTGGTATGCTGGCCCAAT
GGAAAGATTGCAGGCAGAAACTGAACTTATTAACAGGGTGAATAGTACTTACCTTGTAAGGCACAGGACC
AAAGAATCTGGAGAATATGCAATTAGCATTAAGTACAATAATGAAGCAAAACACATCAAGATTTTAACAA
GAGATGGCTTTTTTCACATTGCAGAAAATAGAAAATTTAAAAGCTTAATGGAACTTGTGGAGTACTACAA
GCACCATTCTCTCAAAGAAGGGTTCAGGACCTTAGATACAACGCTACAGTTTCCATACAAGGAGCCAGAA
CAGCCAGCTGGACAGAGGGGTAATAGAACAGGCAACAGCTTGTTGAGTCCCAAAGTACTGGGTATTGCCA
TCGCTCGGTACGACTTCTGTGCAAGAGATATGCGGGAGCTCTCCCTATTGAAAGGAGACATGGTGAAGAT
TTACACGAAGATGAGTGCAAATGGCTGGTGGAGAGGTGAAGTAAATGGCAGGGTGGGCTGGTTTCCATCC
ACATATGTGGAAGAAGATGAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210938 representing BC052739
Red=Cloning site Green=Tags(s)

MEPWKQCAQWLIHSKVLPPNHRVTWDSAQVFDLAQTLRDGVLLCQLLNNLRPHSINLKEINLRPQMSQFL
CLKNIRTFLAACCDTFGMRKSELFEAFDLFDVRDFGKVIETLSRLSRTPIALATGIRPFPTEESINDEDI
YKGLPDLIDETRVEDEEDLYDCVYGEDEGGEVYEDLMKAEEAQQPKSQENDIRSCCLAEIRQTEEKYTET
LESIEKYFMAPLKRFLTAAEFDSVFINIPDLVKVHRSLMQEIHDSIVNKDDQNLYQVFINYKERLVIYGQ
YCSGVESAISNLDYISKTKEDVKLKLEECSKRANNGKFTLRDLLVVPMQRVLKYHLLLQELVKHTHDPME
KANLKLALDAMKDLAQYVNEVKRDNETLREIKQFQLSIENLNQPVLLFGRPQGDGEIRITTLDKHTKQER
HIFLFDLAVIVCKRKGDNYEMKEIIDLQQYKIANNPTTDKENKKWSYGFYLIHTQGQNGLEFYCKTKDLK
KKWLEQFEMALSNIRPDYADSNFHDFKMHTFTRVTSCRVCQMLLRGTFYQGYLCFKCGAKAHKECLGRVD
NCGRVNSVEQGPFKPPEKRTNGLRRASRQVDPGLPKMQVIRNYTGTPAPGLHEGPPLHIQAGDTVELLRG
DAHSVFWQGRNLASGEVGFFPSDAVKPSPCVPKPVDYSCQPWYAGPMERLQAETELINRVNSTYLVRHRT
KESGEYAISIKYNNEAKHIKILTRDGFFHIAENRKFKSLMELVEYYKHHSLKEGFRTLDTTLQFPYKEPE
QPAGQRGNRTGNSLLSPKVLGIAIARYDFCARDMRELSLLKGDMVKIYTKMSANGWWRGEVNGRVGWFPS
TYVEEDE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC052739
ORF Size 2543 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052739, AAH52739
RefSeq Size 4770 bp
RefSeq ORF 2543 bp
Locus ID 57257
Gene Summary Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases (By similarity). Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3-mediated adhesion. Does not affect integrin beta-1-mediated adhesion.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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