Ars2 (BC066831) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	ASR2A, ASR2B, ASR2C, ASR2D, Asr2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211012 representing BC066831 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGACAGTGATGATGAATACGACCGAAGACGCAGGGACAAATTTCGAAGAGAGCGCAGCGATTATG ACCGTTCCCGGGAAAGGGATGAAAGACGGCGAGGGGACGATTGGAATGACCGAGAGTGGGACCGTGGCCG GGAGCGCCGCAGTCGGGGTGAATATCGAGACTACGACAGGAACCGAAGGGAGCGCTTCTCTCCCCCTCGA CACGAACTAAGCCCCCCCCAGAAGCGCATGCGGAGAGACTGGGATGAGCACAGCTCTGACCCATACCACA GTGGCTATGACATGCCCTATGCTGGGGGGGGTGGGGGACCAACTTACGGCCCCCCTCAGCCCTGGGGCCA CCCAGACGTCCACATCATGCAGCACCATGTCCTGCCCATCCAGGCCAGGCTGGGCAGCATCGCAGAGATT GACTTGGGGGTGCCACCGCCCATAATGAAGTCCTTCAAAGAGTTCCTCCTGTCTCTGGATGACTCTGTGG ATGAGACAGAGGCAGTTAAACGCTACAATGACTACAAGCTGGACTTCCGAAGGCAGCAGATGCAGGACTT TTTCCTGGCTCACAAAGACGAGGAGTGGTTCCGATCTAAGTACCACCCTGATGAGGTGGGAAAGCGTCGG CAGGAGGCCCGGGGGGCCCTGCAGAACCGGCTGAAGGTGTTCCTGTCCCTCATGGAGAGTGGCTGGTTTG ATAACCTTCTCTTGGACATAGACAAAGCTGATGCCATTGTCAAGATGCTAGATGCAGCTGTCATTAAGAT GGAAGGTGGCACAGAGAACGATCTCCGAATTTTGGAGCAGGAGGAGGAGGAGGAACAGGCAGGCAAGACT GGGGAGGCCAGCAAGAAAGAGGAGGCCCGTGCTGGACCAGCCCTGGGAGAAGGAGAGCGCAAAGCCAATG ATAAGGATGAGAAGAAAGAAGATGGAAAACAGGCTGAGAATGACAGTTCCAACGATGACAAAACTAAAAA ATCTGAGGGTGATGGGGACAAGGAGGAGAAGAAAGAAGAGGCTGAGAAGGAAGCCAAAAAGAGCAAGAAG CGGAACAGGAAGCAGAGTGGCGATGACAGCTTCGATGAGGGCAGTGTGTCCGAGTCTGAGTCCGAGTCTG AGGGTGGCCAGGCCGAGGAGGAGAAGGAGGAGGCCGAAGAAGCACTTAAAGAAAAGGAGAAGCCCAAAGA GGAGGAGAAGGAGAAGCCTAAGGATGCTGCAGGGTTGGAGTGTAAGCCCCGGCCCTTGCATAAGACTTGC TCTCTCTTCATGCGCAACATCGCACCCAACATTTCAAGGGCAGAGATCATTTCTCTTTGTAAACGATACC CAGGCTTTATGCGAGTGGCACTGTCAGAGCCCCAGCCAGAGAGGAGGTTTTTTCGCCGTGGCTGGGTGAC TTTTGACCGCAGTGTTAACATTAAGGAGATCTGTTGGAACCTGCAGAACATTCGGCTCCGGGAGTGTGAA CTGAGTCCCGGTGTGAACAGAGACCTGACCCGTCGTGTCCGCAACATAAATGGCATTACACAGCACAAGC AGATAGTGCGCAATGACATCAAGTTGGCAGCCAAGCTAATCCACACACTGGATGACAGGACCCAGCTCTG GGCCTCTGAGCCTGGGACGCCTCCTGTGCCCACAAGCCTCCCCTCGCAAAACCCCATCCTGAAGAACATC ACTGACTACCTGATTGAGGAAGTGAGTGCGGAGGAGGAGGAGCTTCTGGGGAGCAGTGGGGGACCCCCTC CTGAGGAGCCTCCCAAGGAGGGCAACCCAGCCGAGATCAACGTGGAGAGAGATGAGAAGCTGATCAAGGT CTTGGATAAACTTCTTCTCTATTTGCGTATTGTGCATTCTCTGGATTATTATAACACCTGTGAGTACCCT AATGAAGACGAGATGCCCAACCGCTGTGGCATAATCCACGTTCGGGGGCCCATGCCTCCCAACCGAATTA GTCACGGAGAAGTGCTGGAGTGGCAGAAGACATTTGAGGAGAAACTGACTCCACTGTTGAGTGTGCGTGA ATCCCTTTCTGAGGAAGAGGCCCAGAAGATGGGTCGAAAAGACCCAGAGCAGGAAGTGGAGAAGTTTGTC ACCTCCAACACGCAGGAACTGGGCAAGGATAAGTGGCTATGTCCTCTCAGTGGCAAGAAATTCAAGGGCC CGGAGTTTGTGCGCAAGCATATCTTCAATAAGCATGCCGAGAAGATCGAGGAGGTGAAGAAGGAGGTGGC GTTCTTCAATAACTTTCTCACAGACGCCAAGCGCCCAGCTTTGCCTGAGATCAAGCCAGCTCAGCCACCT GGCCCTGCCCAGATACTCCCCCCAGGCCTGACCCCAGGACTTCCCTACCCACATCAGACGCCACAGGGCT TGATGCCATATGGTCAGCCCCGGCCTCCCATCTTGGGCTATGGAGTCCCAACAGGAGGGCCTCCATACCC CCATGCTCCATATGGTGCCGGCCGTGGGAACTATGATGCTTTTCGAGGCCAAGGCGGTTATCCTGGGAAA CCTCGGAACAGGATGGTTCGAGGAGACCCAAGGGCCATAGTGGAGTATCGGGACCTGGATGCCCCGGATG ATGTTGACTTCTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211012 representing BC066831 Red=Cloning site Green=Tags(s) MGDSDDEYDRRRRDKFRRERSDYDRSRERDERRRGDDWNDREWDRGRERRSRGEYRDYDRNRRERFSPPR HELSPPQKRMRRDWDEHSSDPYHSGYDMPYAGGGGGPTYGPPQPWGHPDVHIMQHHVLPIQARLGSIAEI DLGVPPPIMKSFKEFLLSLDDSVDETEAVKRYNDYKLDFRRQQMQDFFLAHKDEEWFRSKYHPDEVGKRR QEARGALQNRLKVFLSLMESGWFDNLLLDIDKADAIVKMLDAAVIKMEGGTENDLRILEQEEEEEQAGKT GEASKKEEARAGPALGEGERKANDKDEKKEDGKQAENDSSNDDKTKKSEGDGDKEEKKEEAEKEAKKSKK RNRKQSGDDSFDEGSVSESESESEGGQAEEEKEEAEEALKEKEKPKEEEKEKPKDAAGLECKPRPLHKTC SLFMRNIAPNISRAEIISLCKRYPGFMRVALSEPQPERRFFRRGWVTFDRSVNIKEICWNLQNIRLRECE LSPGVNRDLTRRVRNINGITQHKQIVRNDIKLAAKLIHTLDDRTQLWASEPGTPPVPTSLPSQNPILKNI TDYLIEEVSAEEEELLGSSGGPPPEEPPKEGNPAEINVERDEKLIKVLDKLLLYLRIVHSLDYYNTCEYP NEDEMPNRCGIIHVRGPMPPNRISHGEVLEWQKTFEEKLTPLLSVRESLSEEEAQKMGRKDPEQEVEKFV TSNTQELGKDKWLCPLSGKKFKGPEFVRKHIFNKHAEKIEEVKKEVAFFNNFLTDAKRPALPEIKPAQPP GPAQILPPGLTPGLPYPHQTPQGLMPYGQPRPPILGYGVPTGGPPYPHAPYGAGRGNYDAFRGQGGYPGK PRNRMVRGDPRAIVEYRDLDAPDDVDFF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC066831
ORF Size	2606 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC066831, AAH66831
RefSeq Size	2990 bp
RefSeq ORF	2606 bp
Locus ID	83701
Gene Summary	Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Independently of its activity on miRNAs, necessary and sufficient to promote neural stem cell self-renewal. Does so by directly binding SOX2 promoter and positively regulating its transcription.[UniProtKB/Swiss-Prot Function]

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