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Pdcd6ip (NM_011052) Mouse Tagged ORF Clone
CAT＃: MG211015
- TrueORF®
Pdcd6ip (tGFP-tagged) - Mouse programmed cell death 6 interacting protein (Pdcd6ip)
ORF Plasmid: DDK
"NM_011052" in other vectors (4)
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CNY 9,500.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI480591; Aip1; Alix; AW544830; C76364; Eig2; mKIAA1375 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211015 representing NM_011052
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGTTCATCTGGGTGCAGCTGAAGAAGACCTCGGAGGTGGACCTGGCCAAGCCGCTGGTGAAGT TCATCCAGCAGACGTACCCGAGCGGCGGCGAGGAGCAGGCCCAGTACTGCCGTGCGGCCGAGGAGCTCAG CAAACTGCGGCGCTCGGCGCTCGGTCGCCCGCTGGACAAGCATGAGGGCGCCCTGGAGACGTTGCTGAGG TATTATGATCAGATTTGTTCCATTGAACCCAAGTTCCCATTTTCTGAAAATCAGATCTGCTTGACGTTCA CGTGGAAGGATGCTTTTGATAAAGGTTCCCTTTTTGGAGGGTCTGTAAAATTGGCTCTTGCAAGCTTAGG ATATGAAAAGAGCTGTGTGTTGTTCAATTGTGCTGCCTTAGCTAGCCAGATTGCAGCAGAGCAGAACCTG GATAATGATGAAGGATTGAAAACCGCTGCTAAGCAGTACCAGTTTGCTAGTGGTGCCTTTTTACATATTA AAGACACAGTGTTATCTGCCTTAAGTCGAGAGCCTACTGTGGACATATCTCCAGATACTGTTGGAACTCT CAGTCTTATTATGCTGGCTCAAGCTCAAGAAGTATTTTTCTTAAAAGCCACAAGAGATAAGATGAAGGAT GCCATCATAGCTAAGCTGGCAAATCAGGCTGCGGATTACTTTGGCGATGCTTTCAAGCAGTGTCAGTACA AGGACACGCTCCCCAAGGAGGTATTCCCCACCCTGGCTGCAAAGCAGTGCATCATGCAGGCCAATGCTGA GTACCACCAGTCCATCCTGGCCAAGCAGCAGAAGAAGTTTGGGGAAGAGATCGCAAGGTTGCAGCACGCA GCAGAACTGATCAAGAATGTGGCCTCTCGCTATGATGAGTATGTCAATGTGAAGGATTTTTCTGACAAAA TCAACCGTGCCCTTACTGCAGCAAAGAAGGATAATGATTTTATTTATCATGACCGTGTTCCGGACCTTAA GGATCTGGATCCTATCGGCAAAGCCACACTTGTGAAGCCCACCCCAGTCAATGTACCTGTCAGCCAGAAG TTCACGGATTTGTTTGAGAAGATGGTCCCTGTGTCTGTGCAGCAGTCCCTGGCTGTGTTTAGTCAGAGGA AAGCTGACTTGGTCAACAGATCAATCGCTCAGATGAGAGAAGCTACGACTTTGGCAAATGGAGTATTGGC TTCCCTTAACCTTCCAGCAGCAATTGAAGATGTGTCTGGAGACACTGTACCTCAGTCTATACTTACCAAG TCTACATCTGTAGTTGAACAGGGAGGCATCCAGACTGTCGACCAGCTGATAAAAGAGCTACCTGAGCTGC TGCAAAGAAATAGAGAAATATTAGAGGAGTCGCTAAGATTGTTGGATGAAGAAGAAGCAACTGACAATGA TTTAAGAGCAAAATTCAAGGACCGCTGGCAAAGGACTCCATCCAATGACCTGTACAAGCCTTTACGAGCA GAGGGAGCCAAATTCAGAGCCGTTTTAGATAAAGCTGTGCAAGCGGATGGACAGGTGAAGGAGCGCTACC AGTCCCATCGAGACACCATCGCACTTCTGTGTAAGCCGGAGCCAGAGCTGAATGCTGCCATCCCCTCTGC TAACCCAGCAAAGACCATGCAGGGCAGCGAGGTTGTAAGTGTCTTAAAGTCCTTATTATCAAATCTTGAT GAAATCAAGAAGGAAAGAGAGAGTCTTGAGAATGACCTGAAGTCAGTGAATTTTGACATGACAAGCAAGT TTTTGACAGCTCTGGCCCAAGATGGCGTGATAACTGAGGAGGCTCTCTCTGTCACTGAGCTGGATCGGAT CTATGGCGGTCTAACAAGTAAAGTTCAAGAGTCTCTGAAGAAACAAGAGGGACTTCTAAAAAATATACAG GTCTCACACCAAGAATTCTCCAAAATGAAGCAATCTAACAACGAGGCTAACTTGAGAGAAGAAGTTCTGA AGAACCTAGCAACTGCGTATGACAACTTTGTTGAGCTTGTAGCTAACTTGAAGGAGGGCACAAAGTTTTA CAATGAGCTGACTGAGATCCTGGTCAGGTTCCAGAACAAATGCAGTGACATAGTGTTTGCACGGAAGACA GAAAGAGACGAGCTCTTGAAGGATCTGCAGCAGAGCATTGCCAGAGAGCCCAGCGCTCCTTCAATCCCTC CTCCAGCCTATCAGTCCTCCCCAGCAGCGGGGCATGCAGCAGCGCCTCCAACTCCAGCCCCAAGAACCAT GCCGCCTGCTAAGCCCCAGCCTCCAGCCCGGCCTCCACCTCCTGTGCTTCCTGCAAACCGAGTTCCTCCT GCTTCTGCTGCTGCTGCCCCTGCAGGCGTGGGGACGGCTTCAGCAGCGCCGCCACAGACCCCTGGCTCTG CTCCCCCGCCACAGGCTCAGGGACCACCATACCCTACCTATCCAGGATATCCCGGGTATTGCCAAATGCC CATGCCCATGGGCTACAACCCCTACGCATATGGCCAGTACAATATGCCGTACCCACCGGTGTATCACCAG AGCCCCGGACAGGCTCCATACCCAGGACCCCAGCAGCCTACCTACCCCTTCCCTCAGCCCCCGCAGCAGT CCTACTATCCACAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211015 representing NM_011052
Red=Cloning site Green=Tags(s) MASFIWVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLRRSALGRPLDKHEGALETLLR YYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLFGGSVKLALASLGYEKSCVLFNCAALASQIAAEQNL DNDEGLKTAAKQYQFASGAFLHIKDTVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKD AIIAKLANQAADYFGDAFKQCQYKDTLPKEVFPTLAAKQCIMQANAEYHQSILAKQQKKFGEEIARLQHA AELIKNVASRYDEYVNVKDFSDKINRALTAAKKDNDFIYHDRVPDLKDLDPIGKATLVKPTPVNVPVSQK FTDLFEKMVPVSVQQSLAVFSQRKADLVNRSIAQMREATTLANGVLASLNLPAAIEDVSGDTVPQSILTK STSVVEQGGIQTVDQLIKELPELLQRNREILEESLRLLDEEEATDNDLRAKFKDRWQRTPSNDLYKPLRA EGAKFRAVLDKAVQADGQVKERYQSHRDTIALLCKPEPELNAAIPSANPAKTMQGSEVVSVLKSLLSNLD EIKKERESLENDLKSVNFDMTSKFLTALAQDGVITEEALSVTELDRIYGGLTSKVQESLKKQEGLLKNIQ VSHQEFSKMKQSNNEANLREEVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIVFARKT ERDELLKDLQQSIAREPSAPSIPPPAYQSSPAAGHAAAPPTPAPRTMPPAKPQPPARPPPPVLPANRVPP ASAAAAPAGVGTASAAPPQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQ SPGQAPYPGPQQPTYPFPQPPQQSYYPQQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011052 |
| ORF Size | 2607 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011052.1, NP_035182.1 |
| RefSeq Size | 2610 bp |
| RefSeq ORF | 2610 bp |
| Locus ID | 18571 |
| UniProt ID | Q9WU78 |
| Gene Summary | Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (By similarity). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (PubMed:27336173).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201554 | Pdcd6ip (untagged) - Mouse programmed cell death 6 interacting protein (Pdcd6ip), transcript variant 3, (10ug) |
CNY 6,376.00 |
|
| MR211015 | Pdcd6ip (Myc-DDK-tagged) - Mouse programmed cell death 6 interacting protein (Pdcd6ip), transcript variant 3 |
CNY 8,650.00 |
|
| MR211015L3 | Lenti ORF clone of Pdcd6ip (Myc-DDK-tagged) - Mouse programmed cell death 6 interacting protein (Pdcd6ip), transcript variant 3 |
CNY 10,550.00 |
|
| MR211015L4 | Lenti ORF clone of Pdcd6ip (mGFP-tagged) - Mouse programmed cell death 6 interacting protein (Pdcd6ip), transcript variant 3 |
CNY 10,550.00 |
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