Abr (BC058708) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211021 representing BC058708 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCGGGGGCCGCCGCCGGCGCCCACTGCGCTACCAGTCCCTGGCAGCCCTGGTGGAAGACTCTC AGTGGCCTTTCTTGTTCCTTGTCTCAGACTTCAGCTACGGTGCGGAGGACTACGACGCAGAGGGGCATGA GGAGCAGAAGGGGCCCCCCGAGGGCTCGGAGACCATGCCGTACATCGACGAGTCACCCACCATGTCGCCC CAGCTCAGCGCCCGCAGCCAAGGTGGTGGGGACAGCGTCTCGCCCACCCCACCTGAGGGATTGGCACCTG GGGTGGAGGCAGGGAAGGGTCTGGAGATGAGGAAGCTAGTCCTCTCTGGGTTTCTGGCCAGTGAAGAGAT CTACATTAACCAGCTGGAAGCTCTGTTACTGCCCATGAAACCCCTGAAGGCCACAGCCACCACCTCCCAG CCTGTGCTCACCATCCAACAGATTGAGACCATCTTCTACAAGATCCAGGACATCTACGAGATCCACAAGG AGTTCTACGACAACCTCTGCCCCAAGGTGCAGCAGTGGGACAGCCAGGTCACCATGGGCCATCTTTTCCA GAAACTGGCCAGCCAGCTTGGCGTGTACAAAGCATTTGTGGATAACTATAAAGTCGCTCTGGAGACAGCC GAGAAGTGCAGCCAGTCCAACAACCAGTTCCAGAAGATCTCTGAGGAACTCAAAGTGAAAGGGCCCAAGG ACTCTAAGGACAGCCATACATCAGTCACCATGGAAGCTCTGCTGTACAAGCCAATTGACCGAGTCACCCG GAGCACCCTGGTCCTTCACGATCTGTTGAAGCACACACCCGTGGACCACCCGGACTACCCGCTGCTTCAG GATGCTCTCCGCATCTCCCAGAATTTCCTGTCTAGCATTAACGAGGACATTGATCCTCGCCGGACTGCAG TCACAACCCCCAAGGGAGAGACTCGGCAGCTGGTGAAGGACGGCTTCCTGGTGGAAATGTCTGAGAGCTC CCGGAAGCTGCGGCATGTCTTTCTCTTCACAGATGTTCTGCTGTGTGCCAAGCTAAAGAAGACCTCAGCA GGAAAGCATCAGCAGTATGACTGTAAGTGGTACATCCCTCTGGCTGACTTAGTGTTCCCATCCCCTGAGG AGTCAGAGGCCAGTCCCCAGGTGCACCCCTTCCCAGACCACGAGCTCGAGGACATGAAAACGAAGATCTC TGCCCTCAAGAGTGAGATCCAGAAGGAGAAAGCCAACAAAGGACAGAGCCGAGCCATTGAGCGCCTCAAA AAGAAGATGTTTGAGAATGAGTTCTTACTGCTGCTCAATTCCCCAACGATCCCTTTCCGGATACACAATC GGAATGGAAAGAGTTACCTGTTTCTTCTGTCTTCTGACTATGAGAGGTCGGAGTGGAGAGAAGCGATTCA GAAGCTACAGAAGAAGGATCTCCAGGCCTTTGTCCTGAGCTCTGTGGAGCTCCAGGTGCTCACGGGATCC TGTTTCAAGCTTAGGACTGTGCACAACATTCCTGTCACCAGCAATAAAGATGATGATGAGTCTCCAGGAC TGTACGGCTTCCTTCATGTCATCGTCCACTCTGCCAAGGGCTTTAAACAGTCAGCCAATCTTTACTGTAC TCTGGAGGTGGATTCCTTTGGCTATTTTGTCAGCAAAGCCAAGACCAGGGTGTTTCGGGACACGACAGAG CCCAAGTGGGATGAGGAGTTTGAGATTGAGCTGGAGGGTTCACAGTCCCTGAGGATCCTGTGTTATGAGA AGTGCTACGACAAGACCAAGGTCAACAAAGACAACAATGAGATTGTGGACAAGATCATGGGCAAGGGGCA GATCCAGCTGGATCCACAGACAGTAGAAAGCAAGAACTGGCATACGGACGTGATTGAAATGAATGGGATC AAAGTGGAATTCTCCATGAAATTTACCAGCCGTGACATGAGCCTGAAGAGGACCCCATCCAAAAAGCAGA CAGGCGTCTTTGGAGTGAAGATCAGCGTGGTGACCAAGCGGGAGCGCTCCAAGGTGCCCTACATCGTCCG GCAATGCATAGAAGAGGTGGAGAAGAGGGGCATTGAGGAGGTTGGCATCTACAGGATATCAGGGGTGGCC ACGGACATCCAGGCCCTGAAGGCTGTCTTTGATGCCAATAACAAGGACATCCTACTGATGCTGAGCGACA TGGACATCAACGCCATCGCTGGGACCCTCAAGCTATACTTTCGGGAGCTGCCTGAGCCCCTCCTTACAGA TAGACTTTATCCAGCCTTCATGGAGGGCATTGCCCTGTCAGACCCTGCTGCCAAGGAGAACTGCATGATG CACCTACTCCGCTCCCTGCCTGACCCCAACCTCATCACCTTCCTTTTCCTGCTGGAACACTTGAAAAGGG TTGCTGAGAAGGAGCCCATCAACAAGATGTCACTTCACAACCTTGCCACGGTGTTTGGCCCCACGTTACT GAGACCATCAGAAGTGGAGAGCAAAGCACATCTCACATCAGCTGCAGACATCTGGTCCCATGATGTCATG GCCCAGGTCCAGGTCCTCCTCTACTACCTGCAGCATCCCCCCATTTCCTTCGCAGAACTGAAGCGGAACA CACTGTACTTCTCCACAGACGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211021 representing BC058708 Red=Cloning site Green=Tags(s) MAAGGRRRRPLRYQSLAALVEDSQWPFLFLVSDFSYGAEDYDAEGHEEQKGPPEGSETMPYIDESPTMSP QLSARSQGGGDSVSPTPPEGLAPGVEAGKGLEMRKLVLSGFLASEEIYINQLEALLLPMKPLKATATTSQ PVLTIQQIETIFYKIQDIYEIHKEFYDNLCPKVQQWDSQVTMGHLFQKLASQLGVYKAFVDNYKVALETA EKCSQSNNQFQKISEELKVKGPKDSKDSHTSVTMEALLYKPIDRVTRSTLVLHDLLKHTPVDHPDYPLLQ DALRISQNFLSSINEDIDPRRTAVTTPKGETRQLVKDGFLVEMSESSRKLRHVFLFTDVLLCAKLKKTSA GKHQQYDCKWYIPLADLVFPSPEESEASPQVHPFPDHELEDMKTKISALKSEIQKEKANKGQSRAIERLK KKMFENEFLLLLNSPTIPFRIHNRNGKSYLFLLSSDYERSEWREAIQKLQKKDLQAFVLSSVELQVLTGS CFKLRTVHNIPVTSNKDDDESPGLYGFLHVIVHSAKGFKQSANLYCTLEVDSFGYFVSKAKTRVFRDTTE PKWDEEFEIELEGSQSLRILCYEKCYDKTKVNKDNNEIVDKIMGKGQIQLDPQTVESKNWHTDVIEMNGI KVEFSMKFTSRDMSLKRTPSKKQTGVFGVKISVVTKRERSKVPYIVRQCIEEVEKRGIEEVGIYRISGVA TDIQALKAVFDANNKDILLMLSDMDINAIAGTLKLYFRELPEPLLTDRLYPAFMEGIALSDPAAKENCMM HLLRSLPDPNLITFLFLLEHLKRVAEKEPINKMSLHNLATVFGPTLLRPSEVESKAHLTSAADIWSHDVM AQVQVLLYYLQHPPISFAELKRNTLYFSTDV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058708
ORF Size	2613 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058708, AAH58708
RefSeq Size	5263 bp
RefSeq ORF	2615 bp
Locus ID	109934
Gene Summary	Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (By similarity). Functions as an important negative regulator of neuronal RAC1 activity (PubMed:20962234). Regulates macrophage functions such as CSF-1 directed motility and phagocytosis through the modulation of RAC1 activity (PubMed:17116687).[UniProtKB/Swiss-Prot Function]

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