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Bcar1 (NM_009954) Mouse Tagged ORF Clone
CAT＃: MG211033
- TrueORF®
Bcar1 (tGFP-tagged) - Mouse breast cancer anti-estrogen resistance 1 (Bcar1)
ORF Plasmid: DDK
"NM_009954" in other vectors (4)
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CNY 9,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI385681; Cas; Crkas |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211033 representing NM_009954
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGTGCCGAACGTGCTGGCCAAAGCCCTCTATGACAATGTTGCTGAATCCCCGGATGAGCTCTCCT TCCGAAAGGGTGACATCATGACAGTGCTCGAGCGGGACACTCAGGGCCTGGATGGCTGGTGGCTCTGCTC ACTGCATGGGCGCCAGGGCATTGTGCCTGGTAACCGCCTCAAGATTCTGGTTGGCATGTATGATAAGAAG CCAGTAGGACCTGGACCAGGCCCACCTGCCACCCCACCCCAGCCCCAGCCCAGCCTTCCCCAGGGCGTCC ATGCTCCGGTACCTCCAGCTTCCCAGTACAGTCCCATGCTCCCCACTGCGTACCAGCCCCAGTCTGACAA TGTGTACCTGGTACCCACTCCCAGCAAGACTCAGCAAGGTCTCTACCAAGCCCCTGGGCCCAACCCACAG TTCCAGTCACCCCCAGCCAAACAGACGTCCACATTCTCCAAGCAAACACCTCATCACTCATTTCCCAGCC CAGCCACGGACCTTTACCAGGTGCCCCCAGGGCCTGGAAGTCCAGCCCAGGACATTTATCAGGTGCCACC TTCCGCTGGCATAGGGCATGACATCTACCAAGTTCCTCCATCTCTGGACACTCGCGGATGGGAGGGAACA AAGCCACCGGCAAAGGTGGTGGTTCCTACGCGAGTGGGACAGGGCTATGTGTATGAGGCCGCTCAGACGG AGCAGGATGAGTACGACACCCCACGCCACCTGCTGGCCCCAGGTCCCCAGGACATCTATGATGTGCCCCC TGTTCGAGGACTGCTTCCTAACCAGTATGGCCAGGAGGTATATGACACGCCCCCTATGGCAGTCAAAGGC CCCAATGGCCGAGACCCATTGTTGGATGTGTATGACGTGCCCCCTAGTGTGGAGAAAGGCCTGCTCTCGT CCAGCCATCATTCGGTGTATGATGTTCCTCCTTCTGTGAGCAAGGATGTGCCCGATGGCCCACTGCTGCG TGAGGAAACCTATGATGTACCTCCTGCCTTCGCCAAGCCCAAGCCCTTTGATCCCACCCGACACCCACTG ATCCTTGCTGCGCCTCCTCCCGACTCCCCAGCAGCTGAGGATGTATATGATGTGCCCCCCCCTGCTCCCG ACCTCTATGATGTGCCCCCTGGTTTGCGGCGGCCTGGCCCTGGAACACTGTATGACGTGCCTCGCGAGCG CGTGCTCCCTCCTGAGGTGGCTGATGGGAGTGTAGTTGACGATGGTGTGTATGCTGTGCCCCCACCAGCC GAGCGAGAGGCCCCAACGGATGGCAAGCGCCTGTCGGCCTCTAGCACGGGCAGCACGCGCAGCAGCCAAT CGGCATCTTCCTTGGAGGTGGTGGTGCCAGGCCGGGAGCCCCTGGAACTGGAGGTTGCTGTGGAGTCCCT GGCTCGGCTGCAGCAAGGCGTGAGCACCACCGTAGCCCACCTTCTGGACCTGGTGGGCAGTGCCAGTGGG CCTGGGGGCTGGCGTGGTACCTCTGAGCCTCAGGAGCCTCCCGCACAGGACCTGAAAGCCGCAGTGGCGG CAGTTCACGGGGCAGTCCATGAGCTCCTGGAGTTCGCCCGCGGAGCTGTGAGCAATGCCACCCACACTTC TGACCGCACCTTGCATGCCAAGCTTAGCCGGCAACTACAGAAGATGGAGGATGTGTACCAGACACTGGTG GTCCACGGTCAGGTCCTTGACAGTGGCCGAGGAAGTCCAGGATTCACTCCGGAAGACCTGGACCGCCTGG TGGCCTGCTCTCGGGCTGTGCCCGAGGATGCCAAGCAGCTAGCTTCCTTTTTGCATGGCAATGCCTCGCT GCTTTTCAGACGGACCAAAGCCCCTGGCCCAGGGCCTGAGGGAAGCAGCTCCCTGCACCCAAACCCCACT GATAAAGCCAGTAGCATCCAGTCACGCCCTCTCCCCTCACCTCCAAAGTTCACCTCCCAGGACTCTCCAG ACGGCCAGTATGAGAACAGTGAAGGGGGTTGGATGGAGGACTATGACTACGTCCATCTGCAGGGGAAGGA GGAATTTGAGAAGACCCAGAAGGAGCTGCTAGAAAGGGGTAACATCATGCGGCAGGGAAAGGGCCAACTG GAGTTGCAGCAGCTGAAACAGTTTGAGCGACTGGAGCAGGAGGTGTCTCGTCCAATAGACCACGACCTGG CCAACTGGACACCAGCCCAGCCCCTGGTGCCGGGCCGGACAGGGGGCCTGGGGCCTTCAGACCGACAGCT GCTGCTCTTCTACTTGGAGCAGTGCGAGGCCAACCTGACCACACTGACAGATGCAGTGGACGCCTTCTTC ACTGCGGTGGCCACCAACCAACCACCCAAGATCTTTGTGGCACACAGCAAGTTTGTCATTCTCAGTGCCC ACAAGCTTGTGTTCATTGGGGACACACTGTCACGGCAGGCAAAGGCAGCTGATGTCCGAAGCCAAGTGAC CCACTACAGCAATCTGCTGTGTGACCTCCTGCGTGGCATTGTGGCCACCACCAAGGCTGCTGCCCTGCAG TACCCATCCCCTTCCGCTGCCCAGGACATGGTGGACAGGGTCAAGGAGCTAGGCCACAGCACTCAGCAGT TCCGCCGCGTCCTGGGCCAGCTAGCTGCTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211033 representing NM_009954
Red=Cloning site Green=Tags(s) MTVPNVLAKALYDNVAESPDELSFRKGDIMTVLERDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKK PVGPGPGPPATPPQPQPSLPQGVHAPVPPASQYSPMLPTAYQPQSDNVYLVPTPSKTQQGLYQAPGPNPQ FQSPPAKQTSTFSKQTPHHSFPSPATDLYQVPPGPGSPAQDIYQVPPSAGIGHDIYQVPPSLDTRGWEGT KPPAKVVVPTRVGQGYVYEAAQTEQDEYDTPRHLLAPGPQDIYDVPPVRGLLPNQYGQEVYDTPPMAVKG PNGRDPLLDVYDVPPSVEKGLLSSSHHSVYDVPPSVSKDVPDGPLLREETYDVPPAFAKPKPFDPTRHPL ILAAPPPDSPAAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGSVVDDGVYAVPPPA EREAPTDGKRLSASSTGSTRSSQSASSLEVVVPGREPLELEVAVESLARLQQGVSTTVAHLLDLVGSASG PGGWRGTSEPQEPPAQDLKAAVAAVHGAVHELLEFARGAVSNATHTSDRTLHAKLSRQLQKMEDVYQTLV VHGQVLDSGRGSPGFTPEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKAPGPGPEGSSSLHPNPT DKASSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLERGNIMRQGKGQL ELQQLKQFERLEQEVSRPIDHDLANWTPAQPLVPGRTGGLGPSDRQLLLFYLEQCEANLTTLTDAVDAFF TAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQ YPSPSAAQDMVDRVKELGHSTQQFRRVLGQLAAA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009954 |
| ORF Size | 2622 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009954.3, NP_034084.2 |
| RefSeq Size | 3209 bp |
| RefSeq ORF | 2625 bp |
| Locus ID | 12927 |
| UniProt ID | Q61140 |
| Gene Summary | Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration (By similarity). Has been shown to be essential in cardiovascular development during embryogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203449 | Bcar1 (untagged) - Mouse breast cancer anti-estrogen resistance 1 (Bcar1), transcript variant 2, (10ug) |
CNY 9,608.00 |
|
| MR211033 | Bcar1 (Myc-DDK-tagged) - Mouse breast cancer anti-estrogen resistance 1 (Bcar1), transcript variant 2 |
CNY 9,600.00 |
|
| MR211033L3 | Lenti ORF clone of Bcar1 (Myc-DDK-tagged) - Mouse breast cancer anti-estrogen resistance 1 (Bcar1), transcript variant 2 |
CNY 10,640.00 |
|
| MR211033L4 | Lenti ORF clone of Bcar1 (mGFP-tagged) - Mouse breast cancer anti-estrogen resistance 1 (Bcar1), transcript variant 2 |
CNY 10,640.00 |
