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Mcm10 (NM_027290) Mouse Tagged ORF Clone
CAT＃: MG211059
- TrueORF®
Mcm10 (tGFP-tagged) - Mouse minichromosome maintenance deficient 10 (S
ORF Plasmid: DDK
"NM_027290" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2410041F14Rik; AU018508; C79164; C330019M07Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211059 representing NM_027290
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGGAGGAAGACGACTTGTGTCTCCTGACATCTCTACTGGAAGAGAATGAGGCAGTCTTACCTT GCAGCTCAGAAAAGGATAAGTCCTTGTCTCTGGGAGACGGGGACCCTGATGAATTTGATGAGCTCTTTGA TGCTGATCGTGATGGTGAGTCTTACACAGAAGAGGCTGGCAGTGGAGAAGAGGGCAAGACTGGAAACCAG GAGGAACGTTTGGCCACTCTCTTTGGTGATGTGGAGGACTTAACGGATGATGAAGTTGCTACATCGAAAG TTGGAAACAGTGGCCCCCCTCCTGCTCCCAGCCAAGAGAAAACCAGCGAGGAGCTGCAAGATGAATTAAA GAAGTTGCAAGAGCAAATGAAATCCTTACAGGAGCAGCTAAAAGCTGCATCAATTAAACAGCCTCCAGGG ACAGCACCCTTGCAGGAACCACCTGATTCATCTCTGCAGCCACTGCTTAAGGAGAAGAGAATTCGGAGAA TTCAGGAGTCAGCGTGCTTTTCTGCAGAGCTTGATGTCCCCACTTTACCCAAAGCCAAGCGGGTTGCTCG GAAGCCAAAGACTCCTGCAGAGTCATCTTCAAGGATGCGTACACCCGCACAGCCACTACAGGTTTCTTCC AGTTTTCTAGAGCCTAACCACTCCTCTTCTTCGAGATCAAGCACACCCTCACCTCAAGCTGTTCCTGGAA ACAAATGTAGCAGGACAATTAGAAATCAAAACACAGTGTCCCCAGGGAATTCTGGGGACCGGCCCCAGCA GGTTTCCCAGGTCTCTGTGGAAGCCTTCTCAGGCCTACGCCTCAGGCGACCTCGAGTCTCCTCCACAGAA ATGAGCAGGAAGATGGCAGGCCGGAAACTAATCAGACTGCCTCAGATCAAGGAGAAGATGGCAACTGAGA ACCTGGAAGAGACAGACTGGGTGACGTTTGGGGTTATCCTCAGGAAGGTCACTCCACAGAGTGCTACCAG TGGGCAAACGTTTAGCATCTGGAAACTGAACGACCTTCATGACCTGACTCAGTGTGTGTCCTTGTTCTTG TTTGGAGATGTTCACAAAGATCTCTGGAAGACAGAGCAAGGGACCGTCATAGGCTTGCTCAACGCAAACC CCATGAAGCCCAAAGATGGCTTGAAAGAGGTGTGCTTATCTATTGATCATCCTCAAAAAGTCTTAATTAT GGGAGAAGCTATGGACCTGGGAGCCTGTAAAGCCAAGAAGAAGAATGGAGAGCCATGTACACAGACAGTT AACTTGCATGACTGTGAATACTGCCAGTATCACATCCAGGCCCAGTACAAGAAGCTCAGCGCAAAGAGGA CTGATCTGCAGTCCACTTTCTCTGGGGGACGGATTCCGAAGAAGTTCCGCAAAGGCACCAGCCTGAAGGA GCGGCTGTGCCAAGATGGTTTCTACTATGGTGGTGTTTCTTCCGAATCCTTCGCAGCGTCCAGGGCGGCG GCCATTGCTCCTAAGAAGAAGGTTCAAACCACGCTGACCAATCTGGTTGTCCGGGGCACAAACTCGATCA TCCAGGAAACAAAACAAAAGCTTGGTATTCCCCAGAAGAGCTTGTCCTGCTCTGAGGAGTTCAGGGAATT GATGGCCCTGCCCACATTTGGAGCCAGAAACTTACAAAAGCATCTAGCCAGAGCCAAGGCTTCAGGGAGT TCCAAACCTGCCATCCAGTCCATCTCAGCATCAGCCCTCTTGAAGCAGCAGAAACAACAGATGCTGGAGA TGCGGAAGAGGAGATCAGAAGACATACAGAAGCGATTCCTCCAAAGCTCAAGTGAAGTCCAGAGCCCAGC AGTGCCGTCTTCCTCTCGACAGGCTGCTGCCCAGTCTCCTCGAACTGGGGCTGAGTTCCCCAGGTTGGAA GGAACTGCAACCCCACGGATGCCCAAGCTGGGCAGAGGCATCTCAGAAGGGGACGATGTTCTCTTCTTTG ATGATTCACCACCACCAAGACCAAAACTGAGCGCAGCAGCAGAAGCTAAAAAGTTAGCTGCTATTGCAAA ATTAAGGGCAAAAGGCCAGATTCTCACAAAAGTAGATCCAAACAATACTGTAAGGAAGCAAATGGATGGC CGAGCCATGCTGGGAGTGAAGGAGCGTGTGGAGAATAGCAACACAGTTTCTCCTGAAGAGGAGCTGGAGC CTGCCAGGAAAAAAAGGAGAGAGCAGCTTGCCTACTTAGAGTCCGAGGAATTTCAGAAGATTCTAAAAGC AAAGTCCAAGCACACAGACATCCTGAAAGAGGCTGAGGCTGAGCTACAGAAATCCTATTTTGAGCCACTG GTGAAAAAAGAACAGATGGAAGAAAAGATGAGAGCCACCCGGGAAGTGAAATGCCGCGTGGTGACGTGCA GGACGTGCACCTATACCCACTTCAAGCCTCTGGAGACCTGTGTCAGCGAGCAGCATAACCTCCACTGGCA CGACGGCGTGAAGAGGTTTTTCAAATGTCCCTGTGGAAACAGGACCATCTCCCTGGACAAGCTCCCAAAC AAGCACTGCCGTAACTGTGGCCTCTACAAGTGGGAACGGGATGGAATGCTGAAGGAGAAGACAGGTCCAA AGATAGGAGGAGAGACCCTGCTGCCGATAGGGGAAGAGCACGCCAAGTTTCTGAACAGCCTCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211059 representing NM_027290
Red=Cloning site Green=Tags(s) MDVEEDDLCLLTSLLEENEAVLPCSSEKDKSLSLGDGDPDEFDELFDADRDGESYTEEAGSGEEGKTGNQ EERLATLFGDVEDLTDDEVATSKVGNSGPPPAPSQEKTSEELQDELKKLQEQMKSLQEQLKAASIKQPPG TAPLQEPPDSSLQPLLKEKRIRRIQESACFSAELDVPTLPKAKRVARKPKTPAESSSRMRTPAQPLQVSS SFLEPNHSSSSRSSTPSPQAVPGNKCSRTIRNQNTVSPGNSGDRPQQVSQVSVEAFSGLRLRRPRVSSTE MSRKMAGRKLIRLPQIKEKMATENLEETDWVTFGVILRKVTPQSATSGQTFSIWKLNDLHDLTQCVSLFL FGDVHKDLWKTEQGTVIGLLNANPMKPKDGLKEVCLSIDHPQKVLIMGEAMDLGACKAKKKNGEPCTQTV NLHDCEYCQYHIQAQYKKLSAKRTDLQSTFSGGRIPKKFRKGTSLKERLCQDGFYYGGVSSESFAASRAA AIAPKKKVQTTLTNLVVRGTNSIIQETKQKLGIPQKSLSCSEEFRELMALPTFGARNLQKHLARAKASGS SKPAIQSISASALLKQQKQQMLEMRKRRSEDIQKRFLQSSSEVQSPAVPSSSRQAAAQSPRTGAEFPRLE GTATPRMPKLGRGISEGDDVLFFDDSPPPRPKLSAAAEAKKLAAIAKLRAKGQILTKVDPNNTVRKQMDG RAMLGVKERVENSNTVSPEEELEPARKKRREQLAYLESEEFQKILKAKSKHTDILKEAEAELQKSYFEPL VKKEQMEEKMRATREVKCRVVTCRTCTYTHFKPLETCVSEQHNLHWHDGVKRFFKCPCGNRTISLDKLPN KHCRNCGLYKWERDGMLKEKTGPKIGGETLLPIGEEHAKFLNSLK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027290 |
| ORF Size | 2655 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027290.1, NP_081566.1 |
| RefSeq Size | 3510 bp |
| RefSeq ORF | 2658 bp |
| Locus ID | 70024 |
| UniProt ID | Q0VBD2 |
| Gene Summary | Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222381 | Mcm10 (untagged) - Mouse minichromosome maintenance deficient 10 (S. cerevisiae) (Mcm10), (10ug) |
CNY 11,310.00 |
|
| MR211059 | Mcm10 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 10 (S. cerevisiae) (Mcm10) |
CNY 6,488.00 |
|
| MR211059L3 | Lenti ORF clone of Mcm10 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 10 (S. cerevisiae) (Mcm10) |
CNY 9,030.00 |
|
| MR211059L4 | Lenti ORF clone of Mcm10 (mGFP-tagged) - Mouse minichromosome maintenance deficient 10 (S. cerevisiae) (Mcm10) |
CNY 9,030.00 |
