Ppp1r10 (BC052059) Mouse Tagged ORF Clone

CAT#: MG211070

  • TrueORF®

Ppp1r10 (tGFP-tagged) - Mouse protein phosphatase 1, regulatory subunit 10 (cDNA clone MGC:62449 IMAGE:5702171)



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Mouse Protein / Lysate Request

CNY 7,890.00


货期*
4周

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms CAT53, FB19, PNUTS
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211070 representing BC052059
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTTCAGGTCCCATAGACCCCAAAGAACTGCTCAAGGGCCTGGATAGCTTCCTTACCCGGGACGGAG
AAGTGAAGAGTGTGGATGGAATTTCCAAGATCTTCAGTCTAATGAAGGAGGCACGAAAGATGGTGAGTCG
GTGCACGTACTTGAACATAATCCTGCAGACCCGCGCTCCAGAAGTGCTCGTCAAGTTTATTGATGTCGGT
GGCTACAAGCTTCTGAACAACTGGCTAACATATTCAAAGACCACCAACAACATTCCTCTCCTACAGCAGA
TTCTGCTGACTCTGCAGCACCTCCCGCTCACTGTGGACCATCTCAAGCAGAACAACACAGCAAAACTAGT
GAAGCAGCTCAGCAAGTCAAGTGAGGATGAAGAGCTCCGGAAATTGGCATCAGTCCTTGTCAGTGACTGG
ATGGCTGTCATCCGCTCCCAGAGTAGCACCCAGCCTGCAGAGAAAGATAAGAAGAAAAGGAAGGAAGAGG
GGAAAAGTCGAACCACGCTTCCTGAGCGGCCTTTGACTGAAGTGAAGGCTGAGACCCGGGCTGAGGAAGC
CCCAGAGAAGAAGAAAGAGAAGCCCAAGTCACTTAGAACTACGGCACCCAGTCATGCCAAGTTCCGTTCT
ACTGGACTGGAGCTGGACACCCCATCTTTGGTGCCTGTGAAGAAGAACTCCAGCACTGTGGTTGTGTCAG
ACAAGTACAACCTGAAGCCCATCCCCCTGAAGCGGCAGAGTGCCACAGCTGCTCCAGGTGACGCTGCCCC
ACCTGCAGAGAAGAAATACAAGCCTCTCAACACAGCCCCCAATACCACCAAAGAAATCAAAGTGAAGATC
ATCCCTCCACAGCCTATGGAGGGCCTGGGTTTTCTGGATGCTCTCAATTCAGCCCCTGTCCCAGGCATCA
AAATTAAGAAGAAGAAGAAGGTACTATCGCCGACTGCTGCCAAGCCCAGCCCTTTTGAAGGGAAAACAAG
CACCGAGCAGAGCACAGCCAAACCTTCCTCCCCAGAGCCTGCACCACCTGCTGAGCCCATGGACACGGAC
CGCCCTGGCACCCCTGTTCCCCCTGTTGAAGTTCCAGAGCTCATGGATGCAGCCTCTTCAGAGCCAGGAG
CTCTGGATGCAAAGCCTGTGGACAGCCCTGGAGATCCCAACCAGCTGACTCGCAAGGGCAGAAAGAGGAA
AACTGTGACTTGGCCTGAGGAGGGCAAGCTGAGAGAGTATTTCTACTTTGAACTGGACGAAACTGAGCGA
GTGAATGTGAACAAGATCAAAGACTTCGGGGAGGCTGCTAAGCGTGAGATACTGTCTGACCGACATGCTT
TCGAGACAGCTCGGCGACTAAGCCATGACAATATGGAGGAGAAAGTGCCTTGGGTGTGTCCCAGGCCTCT
GGTTCTGCCCTCACCTCTTGTCATCCCTGGAAGCAACAGCCAGGAGCGATACATCCAGGCAGAGCGGGAG
AAGGGCATCCTTCAGGAACTCTTCCTGAACAAGGAGAGTCCACATGAACCTGATCCTGAGCCATATGAAC
CCATTCCCCCAAAACTCATCCCCTTGGATGAGGAATGTGCCATGGATGAGACTCCATATGTCGAGACCCT
GGAGCCTGGAGGGTCTGGTGGCTCACCTGATGGAGCAGGAGGCTCCAAGCTGCCTCCAGTTCTGGCCAAT
CTTATGGGAAGCATGGGAGCTGGAAAGAGCCCCCAGGGCCCTGGAGGAGGCGGCATCAATGTTCAGGAGA
TCCTCACTTCCATAATGGGCAGTCCAAACAGCCACCCTTCAGAGGAGCTGCTGAAGCAGCCTGACTACTC
AGACAAGCTCAAGCAGATGCTGGTGCCTCATGGGCTCCTAGGTCCTGGTCCTGTGGCCAATGGCTTTCCA
CCAGGAGGCCCTGGGGGCCCGAAGGGTATGCAGCATTTCCCCCCTGGTCCTGGAGGGCCCATGCCAGGTC
CCCATGGAGGCCCTGGTGGACCAGTGGGTCCACGTCTCTTAGGTCCCCCACCACCTTCTCGGGGAGGTGA
TCCATTCTGGGATGGCCCAGGTGATCCAATGCGAGGTGGCCCAATGCGTGGGGGTCCAGGACCTGCTCCC
GGACCATACCACAGAGGCCGAGGGGGTCGAGGAGGAAATGAGCCACCGCCACCACCTCCATTCCGAGGAG
CCAGAGGAGGTCGTTCCGGAGGAGGACCACCAAATGGCCGAGGGGGTCCTGGTGGAGGAGGCATGGTTGG
AGGTGGTGGGCACCGCCCCCATGAAGGCCCTGGAGGGAGCATGGGCAGTGGACATCGTTCCCATGATGGC
CCTGGAGGGAACATGGGCAGTGGACATCGTTCTCATGATGGCCCTGGAGGGAACATGGGTGGAAGTGGTG
GACATCGTTCCCATGAAGGCCCTGGACACGGAGGACCCCATGGACACCGACCACATGATGTCCCTAGCCA
TCGAGGCCACGACCATCGAGGGCCACCACCTCATGAACACCGTGGTCATGATGGCCATGGGGGAGGCGGC
CACCGAGGGCATGATGGAGGCCACAGTCATGGAGGAGACATGTCAAACCGCCCTGTCTGCCGACACTTCA
TGATGAAGGGCAACTGCCGCTATGAGAACAACTGTGCCTTCTACCACCCAGGGGTCAATGGGCCCCCACT
GCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211070 representing BC052059
Red=Cloning site Green=Tags(s)

MGSGPIDPKELLKGLDSFLTRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNIILQTRAPEVLVKFIDVG
GYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDW
MAVIRSQSSTQPAEKDKKKRKEEGKSRTTLPERPLTEVKAETRAEEAPEKKKEKPKSLRTTAPSHAKFRS
TGLELDTPSLVPVKKNSSTVVVSDKYNLKPIPLKRQSATAAPGDAAPPAEKKYKPLNTAPNTTKEIKVKI
IPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAKPSPFEGKTSTEQSTAKPSSPEPAPPAEPMDTD
RPGTPVPPVEVPELMDAASSEPGALDAKPVDSPGDPNQLTRKGRKRKTVTWPEEGKLREYFYFELDETER
VNVNKIKDFGEAAKREILSDRHAFETARRLSHDNMEEKVPWVCPRPLVLPSPLVIPGSNSQERYIQAERE
KGILQELFLNKESPHEPDPEPYEPIPPKLIPLDEECAMDETPYVETLEPGGSGGSPDGAGGSKLPPVLAN
LMGSMGAGKSPQGPGGGGINVQEILTSIMGSPNSHPSEELLKQPDYSDKLKQMLVPHGLLGPGPVANGFP
PGGPGGPKGMQHFPPGPGGPMPGPHGGPGGPVGPRLLGPPPPSRGGDPFWDGPGDPMRGGPMRGGPGPAP
GPYHRGRGGRGGNEPPPPPPFRGARGGRSGGGPPNGRGGPGGGGMVGGGGHRPHEGPGGSMGSGHRSHDG
PGGNMGSGHRSHDGPGGNMGGSGGHRSHEGPGHGGPHGHRPHDVPSHRGHDHRGPPPHEHRGHDGHGGGG
HRGHDGGHSHGGDMSNRPVCRHFMMKGNCRYENNCAFYHPGVNGPPLP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC052059
ORF Size 2666 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052059, AAH52059
RefSeq Size 4240 bp
RefSeq ORF 2666 bp
Locus ID 52040
Gene Summary Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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