Nfatc4 (BC028928) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	3110041H08Rik; AW107667; AW546455; Nfat3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211104 representing BC028928 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCCGCAAGCTGCGAGGATGAGGAGCTGGAATTTAAGCTGGTGTTCGGAGAGGAAAAGGAGCCCC CCCCGCTGGGCCCTGGGGGTCCTGGGGAAGAGCTGGACTCAGAGGATACCCCTCCATGCTGTCGTTTGGC CCTGGGGGAACCCCTTCCTTATGGTGCTGCCCCTATTGGGATTCCCCGACCCCCACCCCCTCGGCCAGGG ATGCACTCACCACCGCCCCGTCCCGCCCCTTCACCTGGCACCTGGGAGAGCCAGCCAGCTAGGTCGGTGA GGCTGGGGGGCCCAGGAGGTAATGCAGGGGGTGCTGGGGGTGGTCGTGTTCTTGAGTGTCCCAGCATCCG GATTACCTCCATCTCTCCCACGCCTGACCCGCCGACCTCGCTAGAGGACACTCCTGAAACTTGGGGGGAC GGCTCTCCTAGAGATTACCCTCCACCAGAAGGCTTTGGGGGCTATCGAGAGGCTGGAGGTCAGGGCGGAG GCGCCTTCTTCAGCCCGAGCCCTGGCAGCAGCAGCCTGTCTTCTTGGAGCTTCTTCTCGGACGCCTCCGA CGAGGCAGCCCTGTATGCAGCGTGCGACGAGGTGGAGTCTGAACTTAATGAAGCAGCCTCCCGTTTTGGC TTGAGCTCTCCGCTGCCCTCGCCCCGGGCTTCTCCTAGGCCATGGACCCCGGAGGATCCCTGGAGCCTAT ACGGTCCAAGCTCGGGAGGCCGAGCGCCAGAGGATAGCTGGCTACTCCTCAGCGCTCCTGGGCCCGTCCC AGCCTCCCCTCGGCCTGCTTCTCCCTGCGGCAAGCGGCGCTATTCCAGTTCCGGAACCCCATCTTCGGCC TCCCCAGCTTTGTCCCGCCGAGGCAGCCTTGGAGAAGAAGGTCCAGAGCCACCTCCACCACCCCCGTTGC CTCTGGTCCGGGACCCTAGTTCCCCTGGCCCTTTTGACTATGTGGGTGCTCCACCAACCGAGAGCATCCC TCAGAAAACTCGGAGGACTTCTAGCGAGCAAGCAGTGGCCCTGCCTCGGTCTGAAGAGCCTCCCTCGTGC AATGGGAAGCTCCCCTCCGGTACAGAGGACTCTGTGGCTGCTCCGGGGGCTCTGCGGAAAGAGGTGGCCG GCATGGATTACCTAGCAGTGCCTTCTCCCCTTGCTTGGTCCAAGGCCCGGATTGGGGGACACAGCCCCAT CTTCAGGACCTCTGCCCTACCTCCCCTGGACTGGCCTTTACCCAGCCAGTATGAGCAGCTGGAGCTGAGG ATCGAGGTACAGCCTAGAGCACACCACCGAGCTCACTATGAGACTGAGGGCAGCCGGGGGGCTGTCAAGG CTGCTCCTGGAGGCCACCCTGTGGTCAAGCTCCTAGGCTACAGTGAGAAGCCATTGACTCTGCAGATGTT CATTGGCACTGCAGATGAGAGGAGCCTGCGGCCCCATGCCTTCTACCAGGTGCACCGGATTACTGGCAAG ATGGTGGCTACAGCCAGCTATGAAGCTGTAGTCAGTGGTACCAAAGTGTTGGAGATGACCTTGCTCCCAG AGAACAACATGGCTGCCAACATTGACTGTGCTGGAATCCTGAAGCTTCGAAATTCAGACATTGAGCTACG GAAGGGTGAGACAGACATCGGGCGCAAAAACACGCGTGTGCGGCTGGTGTTCCGTGTGCACGTGCCTCAG GGCAGCGGGAAGGTCGTGTCTGTGCAGGCAGCATCAGTGCCCATCGAGTGCTCCCAGCGCTCTGCCCAGG AGCTGCCCCAGGTGGAGACCTACAGCCCCAGTGCCTGCTCTGTGAGAGGAGGGGAGGAGCTAGTGTTGAC TGGTTCCAACTTCCTGCCAGACTCTAAAGTGGTGTTCATTGAGAGGGGTCCTGATGGAAAACTGCAGTGG GAGGAAGAGGCGGCGGTGAACCGGCTGCAGAGCAGTGAGGTGACATTGACTCTCACCATCCCCGAGTACA GCAACAAGCGGGTGTCCCGACCAGTCCAGGTCTACTTTTACGTCTCCAATGGACGGAGGAAGCGCAGTCC TACCCAAAGTTTCAAGTTCCTACCTGTGGTCTTCAAGGAGGAGCCCCTACCAGACTCATCTCTCCGGGGC TTCCCTTCCACATCGGGTCCCCCCTTTGGCCCTGACGTGGACTTCTCACCGCCCAGGCCCCCTTACCCCT CCTATCCCCATGAAGACCCTGCTTATGAGACTCCTTATCTGTCAGAAGGCTTTGGTTATAGCACACCCGC TCTGTACCCCCAGACGGGGCCCCCACCGTCCTATAGATCTGGCCTGCGGATGTTCCCTGAGACTGGGGGT ACCACAGGTTGTGCCCGCCTACCTTCAGTCTCTTTCCTTCCGCGCCCTTTTCCTGGTGATCAATATGGAG GACAGGGCTCCTCTTTTGCCCTGGGGCTTCCATTCTCCCCTCCAGCCCCCTTTAGGCCGCCACTGCCTTC CTCCCCACCACTCGAAGACCCCTTCCATCCCCAGAGTGCCATCCACCCCTTACCTCCTGAGGGCTACAAT GAGGTGGGGCCAGGCTATACCCCTGGGGAGGGGGCTTCGGAGCAGGAGAAAGCCAGGGGTGGCTACAGCA GCGGCTTCAGAGACAGTGTACCTATCCAGGGTATCACCCTGGAGGAAGTGAGTGAGATCATTGGCCGAGA CCTGAGTGGCTTCCCTGCACGTCCTGGAGAAGAGCCTCCTGCC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211104 representing BC028928 Red=Cloning site Green=Tags(s) MGAASCEDEELEFKLVFGEEKEPPPLGPGGPGEELDSEDTPPCCRLALGEPLPYGAAPIGIPRPPPPRPG MHSPPPRPAPSPGTWESQPARSVRLGGPGGNAGGAGGGRVLECPSIRITSISPTPDPPTSLEDTPETWGD GSPRDYPPPEGFGGYREAGGQGGGAFFSPSPGSSSLSSWSFFSDASDEAALYAACDEVESELNEAASRFG LSSPLPSPRASPRPWTPEDPWSLYGPSSGGRAPEDSWLLLSAPGPVPASPRPASPCGKRRYSSSGTPSSA SPALSRRGSLGEEGPEPPPPPPLPLVRDPSSPGPFDYVGAPPTESIPQKTRRTSSEQAVALPRSEEPPSC NGKLPSGTEDSVAAPGALRKEVAGMDYLAVPSPLAWSKARIGGHSPIFRTSALPPLDWPLPSQYEQLELR IEVQPRAHHRAHYETEGSRGAVKAAPGGHPVVKLLGYSEKPLTLQMFIGTADERSLRPHAFYQVHRITGK MVATASYEAVVSGTKVLEMTLLPENNMAANIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQ GSGKVVSVQAASVPIECSQRSAQELPQVETYSPSACSVRGGEELVLTGSNFLPDSKVVFIERGPDGKLQW EEEAAVNRLQSSEVTLTLTIPEYSNKRVSRPVQVYFYVSNGRRKRSPTQSFKFLPVVFKEEPLPDSSLRG FPSTSGPPFGPDVDFSPPRPPYPSYPHEDPAYETPYLSEGFGYSTPALYPQTGPPPSYRSGLRMFPETGG TTGCARLPSVSFLPRPFPGDQYGGQGSSFALGLPFSPPAPFRPPLPSSPPLEDPFHPQSAIHPLPPEGYN EVGPGYTPGEGASEQEKARGGYSSGFRDSVPIQGITLEEVSEIIGRDLSGFPARPGEEPPA SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC028928
ORF Size	2705 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC028928, AAH28928
RefSeq Size	3075 bp
RefSeq ORF	2705 bp
Locus ID	73181
Gene Summary	Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4 (PubMed:17198697). Along with NFATC3, involved in embryonic heart development (PubMed:12750314, PubMed:17198697). Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function (PubMed:12750314). Transactivates many genes involved in heart physiology. Along with GATA4, binds to and activates NPPB/BNP promoter (PubMed:9568714). Activates NPPA/ANP/ANF and MYH7/beta-MHC transcription (By similarity). Binds to and transactivates AGTR2 gene promoter (PubMed:17198697). Involved in the regulation of adult hippocampal neurogenesis. Involved in BDNF-driven pro-survival signaling in hippocampal adult-born neurons. Involved in the formation of long-term spatial memory and long-term potentiation (PubMed:22586092). In cochlear nucleus neurons, may play a role in deafferentation-induced apoptosis during a developmental critical period when auditory neurons depend on afferent input for survival (PubMed:18354019). Binds to and activates the BACE1/Beta-secretase 1 promoter, hence may regulate the proteolytic processing of the amyloid precursor protein (APP). Plays a role in adipocyte differentiation. May be involved in myoblast differentiation into myotubes (By similarity). Binds the consensus DNA sequence 5'-GGAAAAT-3' (Probable). In the presence of CREBBP, activates TNF transcription. Binds to PPARG gene promoter and regulates its activity (By similarity). Binds to PPARG and REG3G gene promoters (PubMed:17198697).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.