Tubgcp2 (BC012519) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1700022B05Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211127 representing BC012519 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAATTTCGGATTCACCATGATGTCAATGAGCTGCTCAGCCTGCTCCGCATCCATGGCGGGGATG GGGCTGAGGTCTACATTGACCTGCTGCAGAAGAACCGGACGCCGTATGTCACAACCACAGTCTCTGCCCA CAGTGCCAAGGTCAAAATTGCAGAATTTTCTCGAACTCCTGAGGACTTTTTAAAGAAATATGATGAACTG AAATCTAAAAATACAAGGAACCTTGACCCACTGGTGTACCTGTTATCAAAACTCACAGAAGACAAAGAGA CACTTCAGTTTTTGCAACAGAATGCAAAGGAAAGAGCTGAGCTTGCAGCTAGTGCTGCAACTAGCAACAC CACCAGCTTCAGCATCCCTGTTGCAGCCTCTAAGATGTCCACACAAGAGCTAGAGGAACTGAGGAAGCAG CTTGGCAGTGTATCCACAGGCTCCACACTGCAGCAGTCTCTGGAACTTACAAGAAAGATGCTTCGAGACA AACAGAACAAAAAAAATTCAGGCCAGCCCCTCCCTGTCTTCCCAGCATGGGTGTATGAGAGACCAACATT GGCTGGAGATTTCCTGATTGGCTCGGGCTTGAGCTCAGACACAGTTTTGCCTATAGGTACATTGCCCTTG GCCTCCCAGGAGTCAGCTGTGGTGGAAGATCTGCTCTATGTGCTTGTGGGTGTGGATGGCAGGTATATCA CTGCTCAGCCTTTGGCTGGGAGGCAGAACCGTACCTTCCTTGTCGACCCCAACCTGGACCTGTCCATCCG GGAGCTAGTAAACAGAATCCTCCCTGTGGCCGCCAGCTACTCTACTGTGACCAGGTTCATTGAGGAGAAG TCTTCATTTGAATATGGGCAGGTGAACCATGCCCTGGCTGCTGCCATGAGAACCCTAGTAAAGGAATACC TGATTCTGGTCACACAGCTGGAACAGCTGCACAGGCAGGGCCTCCTCTCACTGCAGAAGCTCTGGTTCTA CATCCAGCCAGCCATGCGCACCATCGACATCCTTGCCTCCCTTGCCACCTCAGTGGATAAAGGCGAGTGT GTGGGCGGGTCGACACTGAGCCTTCTTCATGATAGGAGCTTCAACTACACCGGGGACAGCCAGGCGCAGG AGCTGTGCCTCTATCTGACCAAGGCAGCCAGTGCACCTTACTTCGAGATCCTGGAGAAGTGGATCTATAG GGGCATCATTCATGACCCATACAGTGAGTTCATGGTGGAGGAACACGAGCTGCGGAAGGAGAAGATCCAG GAGGACTACAACGACAAGTACTGGGACCAGCGCTACACTGTGCTGCCGCAGCAGATCCCATCCTTCCTGC AGAAGGTAGCAGGCAAGATCCTCAGCACAGGAAAATACCTAAATGTGGTCAGAGAGTGTGGTCATGATGT CACCTGTCCCGTAGCCAAAGAAATCATCTATACCCTTAAAGAGCGGGCATATGTGGAGCAGATAGAGAAA GCGTTCAATTATGCCAGCAAAGTGCTGCTGGACTTCCTCATGGAGGAGAAGGAGCTGGTGGCCCACCTGA GGTCCATCAAGCGCTACTTCCTGATGGACCAGGGGGACTTCTTCGTGCATTTCATGGACCTGACTGAGGA GGAGCTCAGGAAGCCAGTGGAGGACATCATACTCACACGCCTGGAGGCCCTGTTGGAGCTGGCCCTGCGA ATGAGCACTGCCAACACTGACCCCTTCAAAGACGACCTCAAGATTGAACTGATGCCTCACGACCTAATCA CTCAGCTTTTGCGGGTTCTGGCCATCGAGACCAAGCAGGAAAAGGCAATGACCCATGCTGACCCCACGGA GCTCACACTGAGTGGTCTGGAGGCCTTCTCCTTTGACTACATGGTCAAATGGCCCCTGTCACTGATTATT AACAGGAAGGCACTCACCCGCTACCAGATGCTCTTCCGACACATGTTCTACTGCAAGCACGTGGAGCGGC AGCTCTGCAGTGTCTGGATCAGCAACAAGACGGCCAAGCAGCATGCACTGCACTCCGCCAAGTGGTTTGC TGGTGCTTTCACTCTGCGGCAGCGAATGCTCAACTTTGTCCAGAATATTCAGTACTACATGATGTTTGAA GTGATGGAACCGACCTGGCACATCCTTGAGAAAAACCTGAAATCTGCCTCCAACATTGACGATGTCCTTG GCCACCACATAAGCTTCCTTGACAACTGCCTGAAGGACTGCATGCTGACCAACCCTGAGCTGCTGAAGGT TTTCTCCAAGCTCATGTCCGTATGCGTCATGTTTACCAACTGTATGCAGAAATTTACACAGAGTATGAAA CTGGACAGTGAGCTAGGCCGTCTGACACTGGATCAGGGTAGTGTGCAGGGGCCACCCACAGAGACTGAAC GGATTGAGGATCGCCCCCGGAAGGAGCTTACCAGGAAGCATCTGTCTGAGCATGTGGATGCTCCTCAGCT GGCCTCTGGTTTCGAGGCCACCATCAACAACTTTGACAAAAACTTCTCTGCCCATCTTCTTGATTTGCTG GCCCGACTAAGTGTCTACAGCACCAGTGACTGCGAACATGGCATGGCCAGTGTCATCTCCAGACTAGATT TCAATGGTTTCTATGCTGAACGCCTGGAACGTCTGTCTGCAGAAAGGAGTCAGAAGGCTGCCCCCCAAGT GCCTGTCCCACGGGGACCCTCAGCACCAGCACCCAGGGTGGCCATTCCTGCGCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211127 representing BC012519 Red=Cloning site Green=Tags(s) MSEFRIHHDVNELLSLLRIHGGDGAEVYIDLLQKNRTPYVTTTVSAHSAKVKIAEFSRTPEDFLKKYDEL KSKNTRNLDPLVYLLSKLTEDKETLQFLQQNAKERAELAASAATSNTTSFSIPVAASKMSTQELEELRKQ LGSVSTGSTLQQSLELTRKMLRDKQNKKNSGQPLPVFPAWVYERPTLAGDFLIGSGLSSDTVLPIGTLPL ASQESAVVEDLLYVLVGVDGRYITAQPLAGRQNRTFLVDPNLDLSIRELVNRILPVAASYSTVTRFIEEK SSFEYGQVNHALAAAMRTLVKEYLILVTQLEQLHRQGLLSLQKLWFYIQPAMRTIDILASLATSVDKGEC VGGSTLSLLHDRSFNYTGDSQAQELCLYLTKAASAPYFEILEKWIYRGIIHDPYSEFMVEEHELRKEKIQ EDYNDKYWDQRYTVLPQQIPSFLQKVAGKILSTGKYLNVVRECGHDVTCPVAKEIIYTLKERAYVEQIEK AFNYASKVLLDFLMEEKELVAHLRSIKRYFLMDQGDFFVHFMDLTEEELRKPVEDIILTRLEALLELALR MSTANTDPFKDDLKIELMPHDLITQLLRVLAIETKQEKAMTHADPTELTLSGLEAFSFDYMVKWPLSLII NRKALTRYQMLFRHMFYCKHVERQLCSVWISNKTAKQHALHSAKWFAGAFTLRQRMLNFVQNIQYYMMFE VMEPTWHILEKNLKSASNIDDVLGHHISFLDNCLKDCMLTNPELLKVFSKLMSVCVMFTNCMQKFTQSMK LDSELGRLTLDQGSVQGPPTETERIEDRPRKELTRKHLSEHVDAPQLASGFEATINNFDKNFSAHLLDLL ARLSVYSTSDCEHGMASVISRLDFNGFYAERLERLSAERSQKAAPQVPVPRGPSAPAPRVAIPAQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC012519
ORF Size	2717 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC012519, AAH12519
RefSeq Size	2992 bp
RefSeq ORF	2717 bp
Locus ID	74237
Gene Summary	Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.