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Usp4 (BC011341) Mouse Tagged ORF Clone
CAT＃: MG211165
- TrueORF®
Usp4 (tGFP-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165)
ORF Plasmid: DDK
"BC011341" in other vectors (4)
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CNY 5,800.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | F730026I20Rik; mKIAA4155; Unp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211165 representing BC011341
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGGCCGGGGCAGCCGTGAGCGACCGGATGTGGAGACCCAGAAGACGGAGCTCGGAGCCTTGA TGGGGACCACGCTCCAACGTGGGGCGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAA GTATGTGGGCTTTGACAGCTGGGACATGTACAATGTCGGGGAGCATAACCTGTTTCCTGGACCGATTGAC AACTCCGGACTCTTCTCAGATCCTGAGAGTCAGACCTTGAAGGAGCACTTAATCGATGAGCTGGACTATG TGCTGGTCCCAGCCGAAGCCTGGAATAAATTGCTGAATTGGTATGGCTGTGTGGAGGGTCAGCAGCCTAT TGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTGGAAGTGTATTTGCTGGAGCTG AAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTAAGTTGCCATTTTAGCAAAGCAGACACCATTGCAA CTATTGAGAAGGAGATGAGGAAGCTCTTCAACATCCCTGCAGAACGTGAAACACGGCTTTGGAACAAATA CATGAGCAACACCTATGAGCAGTTGAGCAAGCTAGACAACACTATCCAGGATGCTGGGCTGTACCAGGGT CAGGTGCTAGTAATTGAGCCCCAAAATGAAGATGGCACATGGCCCCGGCAGAGCCTGCAGTCAAATGGAT CTGGCTTCTCTGCTTCGTATAATTGCCAGGAGCCCCCATCACCTCATATACAGCCTGGCCTCTGTGGACT TGGAAACCTGGGGAACACCTGCTTCATGAACTCTGCTCTGCAGTGCCTGAGCAACACTGCCCCACTGACT GAGTACTTTCTCAAAGATGAGTATGAGGCCGAGATCAACCGAGACAACCCTCTGGGGATGAAAGGGGAGA TTGCAGAGGCCTATGCAGAGCTCATCAAGCAGATGTGGTCTGGAAGGGACACTCATGTGGCACCACGGAT GTTCAAGACGCAAGTGGGACGTTTTGCCCCTCAGTTTTCTGGCTACCAACAGCAAGACTCCCAGGAGCTG CTAGCCTTTATTCTAGACGGACTGCACGAGGACCTGAACCGCGTAAAGAAGAAGCCTTACCTGGAGCCCA AGGACGCCAATGGGCGACCAGATGCGGTGGTAGCAAAGGAAGCCTGGGAAAACCACAGGCTGAGGAATGA TTCTGTGATTGTGGATACTTTCCATGGCCTTTTCAAATCGACTTTGGTTTGCCCAGAATGTGCTAAAGTT TCTGTGACCTTTGACCCATTTTGCTATCTAACTCTCCCACTGCCTTTGAAGAAGGATCGGATTATGGAGG TCTTCCTGGTTCCTGCTGACCCTCAGTGCAGACCTATCCAGTACCGTGTGACTGTGCCATTGATGGGGGC CATTTCTGACCTGTGTGAAGCACTCTCCAAGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCACTGAT GTATATAATCACCGCTTCCACAAAATTTTCCAAATGGATGAAGGTTTAAGCCACATCACGCCTCGAGATG ACATTTTTGTGTATGAGGTCTGCAACATGTCCATGGATGGCTCAGAGTGTATCACTCTTCCAGTCTACTT CAGAGAGAAGAAGTCCAGGCCATCGAGTGCTTCCTCGGGGGCTGTGCTCTATGGACAGCCCCTTCTTGTC TCTGTCCCTAAGCATAAGCTAACCCTTGAGTCTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTACA TAAAACAGCCTTTGCCTGATGAGTTTCTCAGCTCACCCTTAGAGCCTGGGGCCTGCAATGGCTCTAGGAG TAGCTATGAAGGAGATGAAGAGGAAGAAATGGATCATCAAGAGGAAGGAAAAGAGCAGCTTTCCGAAGTG GAAGGCAGTGGTGAGGACGATCAGGGAGATGACCATAGCGAGAGCGCCCAAAAGGTGAAAGGCCAGCCGA GGCACAAGAGGCTCTTTACCTTCAGCCTCGTGAACTCCTGTGGAACTGCTGATATCAATTCACTGGCAAC TGACGGGAAACTCCTCAAACTCAACTCTCGATCCACACTGGCCATTGACTGGGACAGTGAGACCCGAAGC CTTTACTTTGATGAGCAAGAATCTGAGGCCTGTGAGAAGCACCTGAGCATGTCACAGCCGCAGAAGAAGA AGAAGGCGGCGGTAGCCCTGCGAGAGTGCATCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGCATGA CCCCTGGTACTGTCCCACCTGTAAGAAGCACCAGCAGGCAACAAAGAAGTTTGACTTGTGGTCTTTGCCC AAGATCCTGGTGGTTCACCTCAAGCGTTTCTCCTATAACAGATACTGGCGGGATAAACTTGACACCGTCG TGGAGTTCCCAGTCAGAGCTCTGAACATGTCCGAGTTTGTCTGTGACCGGTCAGCAAGGCCTTATGTTTA TGACCTAATTGCTGTGTCCAATCACTATGGAGCCATGGGGGTTGGTCACTACACTGCATATGCGAAGAAC AGACTGAACGGGAAATGGTATTACTTTGATGATAGCAGTGTGTCCCTGGCCTCTGAGGACCAGATAGTGA CGAAAGCCGCCTACGTGTTGTTCTATCAGCGTCGGGATGACGAATGCTCCAGCACATCTTCGCTCGGCAG TTTCCCCGGTTCTGATGGAGGGGTGAAGCTGAGCAGCTCACACCAGGGCATGGGGGATGAGGAGGCTTAC AACATGGACACCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211165 representing BC011341
Red=Cloning site Green=Tags(s) MAEGRGSRERPDVETQKTELGALMGTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPID NSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLEL KLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTIQDAGLYQG QVLVIEPQNEDGTWPRQSLQSNGSGFSASYNCQEPPSPHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLT EYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQEL LAFILDGLHEDLNRVKKKPYLEPKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKV SVTFDPFCYLTLPLPLKKDRIMEVFLVPADPQCRPIQYRVTVPLMGAISDLCEALSKLSGIAAENMVVTD VYNHRFHKIFQMDEGLSHITPRDDIFVYEVCNMSMDGSECITLPVYFREKKSRPSSASSGAVLYGQPLLV SVPKHKLTLESLYQAVCDRISRYIKQPLPDEFLSSPLEPGACNGSRSSYEGDEEEEMDHQEEGKEQLSEV EGSGEDDQGDDHSESAQKVKGQPRHKRLFTFSLVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRS LYFDEQESEACEKHLSMSQPQKKKKAAVALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLP KILVVHLKRFSYNRYWRDKLDTVVEFPVRALNMSEFVCDRSARPYVYDLIAVSNHYGAMGVGHYTAYAKN RLNGKWYYFDDSSVSLASEDQIVTKAAYVLFYQRRDDECSSTSSLGSFPGSDGGVKLSSSHQGMGDEEAY NMDTN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC011341 |
| ORF Size | 2745 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC011341, AAH11341 |
| RefSeq Size | 3455 bp |
| RefSeq ORF | 2747 bp |
| Locus ID | 22258 |
| Gene Summary | Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220766 | Usp4 (untagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165), (10ug) |
CNY 9,120.00 |
|
| MR211165 | Usp4 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165) |
CNY 6,704.00 |
|
| MR211165L3 | Lenti ORF clone of Usp4 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165) |
CNY 7,130.00 |
|
| MR211165L4 | Lenti ORF clone of Usp4 (mGFP-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165) |
CNY 7,130.00 |
