Hk1 (BC072628) Mouse Tagged ORF Clone

CAT#: MG211174

  • TrueORF®

Hk1 (tGFP-tagged) - Mouse hexokinase 1 (cDNA clone MGC:99852 IMAGE:6832742)



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Mouse Protein / Lysate Request

CNY 6,940.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Hk1-s, mHk1-s
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211174 representing BC072628
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCGCCGCGCAACTACTGGCATATTACTTCACCGAGCTGAAGGATGACCAAGTCAAAAAGATTGATA
AGTATCTGTATGCCATGCGGCTCTCTGATGAAATTCTGATAGATATCCTGACACGCTTCAAGAAAGAGAT
GAAGAATGGCCTCTCCCGGGATTATAACCCAACGGCCTCCGTCAAGATGCTGCCAACCTTTGTCCGGTCC
ATTCCGGACGGCTCAGAAAAGGGGGATTTCATTGCACTGGATCTCGGCGGGTCTTCCTTTCGAATCCTGC
GGGTGCAGGTGAACCACGAGAAGAGTCAGAACGTCAGCATGGAGTCTGAGGTCTACGACACCCCAGAGAA
CATCGTGCACGGCAGTGGAAGCCAGCTTTTTGATCACGTCGCTGAATGCCTCGGAGACTTCATGGAGAAA
AGGAAGATCAAGGACAAGAAATTACCCGTGGGATTCACGTTTTCCTTCCCGTGCCGACAATCCAAAATAG
ACGAGGCCGTACTGATCACGTGGACAAAGCGGTTCAAAGCCAGTGGCGTGGAAGGGGCGGATGTGGTCAA
GCTGCTGAATAAAGCCATTAAGAAGCGAGGGGACTATGACGCTAACATTGTAGCTGTGGTGAATGACACA
GTGGGGACCATGATGACCTGCGGCTACGATGACCAACAGTGTGAAGTCGGCCTGATCATTGGCACTGGCA
CCAATGCTTGCTACATGGAGGAACTGCGACACATCGACCTGGTGGAAGGCGATGAGGGGAGGATGTGTAT
TAACACGGAATGGGGAGCCTTTGGGGATGATGGGTCCCTGGAAGACATTCGAACAGAGTTTGACAGAGAG
TTAGACCGGGGATCCCTCAACCCTGGGAAACAGCTGTTCGAGAAGATGGTGAGCGGCATGTACATGGGGG
AGCTGGTCCGGCTGATCCTGGTGAAGATGGCCAAGGAAAGCCTCTTATTTGAAGGGCGCATTACTCCAGA
GCTGCTCACGAGGGGCAAGTTCACCACTAGCGACGTAGCCGCCATTGAAACGGATAAGGAAGGCGTTCAA
AATGCCAAGGAAATCTTGACCCGCCTGGGAGTGGAGCCGTCTCACGATGACTGCGTATCGGTCCAGCACG
TATGCACGATCGTCTCCTTCCGATCAGCCAACCTGGTGGCTGCCACGCTCGGTGCCATCTTGAACCGCCT
GCGGGACAATAAGGGCACGCCCAGGCTGCGGACCACAGTTGGCGTAGACGGTTCTCTCTACAAGATGCAC
CCACAGTATTCCCGGCGGTTCCACAAGACCCTGAGGCGCCTGGTGCCTGACTCGGACGTCCGGTTCCTCC
TCTCGGAGAGTGGCAGTGGCAAGGGAGCCGCCATGGTGACCGCTGTGGCCTACCGCCTGGCCGAGCAGCA
CCGGCAGATTGAGGAAACCCTGTCCCACTTCCGCCTCAGCAAGCAGGCACTGATGGAGGTGAAGAAGAAG
CTGCGGTCAGAGATGGAAATGGGGCTGAGAAAGGAGACCAACAGCAGAGCTACGGTCAAAATGCTGCCTT
CTTATGTTCGGAGCATCCCAGATGGGACTGAGCATGGTGACTTCCTGGCCTTGGATCTCGGAGGAACGAA
TTTCCGAGTCCTACTGGTAAAGATCCGTAGTGGGAAAAAGAGAACAGTGGAGATGCACAACAAGATCTAC
TCCATTCCCCTGGAAATCATGCAGGGCACCGGGGATGAGCTGTTTGATCACATTGTCTCCTGCATCTCCG
ACTTCCTGGACTACATGGGGATCAAAGGCCCCCGGATGCCTCTGGGCTTCACCTTCTCGTTTCCCTGCAA
GCAGACGAGCCTAGATTGCGGAATCTTGATCACGTGGACAAAGGGATTCAAAGCCACCGACTGTGTGGGT
CACGATGTAGCCACTTTACTGAGGGATGCTGTAAAAAGGAGAGAGGAATTTGACCTGGATGTGGTGGCTG
TGGTCAACGACACCGTGGGCACCATGATGACTTGTGCTTATGAAGAACCTTCTTGTGAGATTGGACTCAT
CGTGGGGACTGGCAGCAATGCCTGCTACATGGAGGAGATGAAAAACGTGGAGATGGTGGAGGGGAACCAG
GGCCAGATGTGCATCAATATGGAATGGGGTGCCTTTGGTGACAACGGCTGTCTGGACGACATCAGAACAG
ACTTCGACAAAGTGGTGGACGAATATTCTCTAAACAGTGGGAAACAAAGGTTTGAGAAGATGATCAGTGG
AATGTACCTGGGTGAGATCGTCCGTAACATCCTGATTGACTTCACCAAGAAAGGCTTCCTCTTCCGGGGA
CAGATCTCTGAGCCACTCAAGACCCGAGGCATCTTCGAGACCAAGTTTCTCTCTCAGATCGAGAGTGACC
GATTAGCGCTGCTCCAGGTGCGGGCCATCCTTCAGCAGCTGGGTCTAAACAGCACGTGCGACGACAGTAT
CCTGGTCAAGACCGTGTGTGGGGTGGTGTCCAAGCGGGCGGCCCAGCTGTGTGGTGCCGGCATGGCCGCC
GTGGTGGAAAAGATCCGAGAGAACAGAGGCCTAGACCACCTGAATGTAACCGTGGGCGTGGACGGGACGC
TCTACAAACTCCATCCACACTTCTCCAGAATCATGCACCAAACAGTGAAGGAACTGTCACCAAAGTGTAC
CGTGTCCTTCCTCCTGTCTGAAGACGGCAGCGGCAAGGGGGCCGCCCTTATCACAGCTGTGGGCGTGCGG
CTCAGAGGAGACCCTACGAACGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211174 representing BC072628
Red=Cloning site Green=Tags(s)

MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDEILIDILTRFKKEMKNGLSRDYNPTASVKMLPTFVRS
IPDGSEKGDFIALDLGGSSFRILRVQVNHEKSQNVSMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEK
RKIKDKKLPVGFTFSFPCRQSKIDEAVLITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDT
VGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDRE
LDRGSLNPGKQLFEKMVSGMYMGELVRLILVKMAKESLLFEGRITPELLTRGKFTTSDVAAIETDKEGVQ
NAKEILTRLGVEPSHDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKMH
PQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLSHFRLSKQALMEVKKK
LRSEMEMGLRKETNSRATVKMLPSYVRSIPDGTEHGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIY
SIPLEIMQGTGDELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCKQTSLDCGILITWTKGFKATDCVG
HDVATLLRDAVKRREEFDLDVVAVVNDTVGTMMTCAYEEPSCEIGLIVGTGSNACYMEEMKNVEMVEGNQ
GQMCINMEWGAFGDNGCLDDIRTDFDKVVDEYSLNSGKQRFEKMISGMYLGEIVRNILIDFTKKGFLFRG
QISEPLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAA
VVEKIRENRGLDHLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCTVSFLLSEDGSGKGAALITAVGVR
LRGDPTNA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC072628
ORF Size 2754 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC072628, AAH72628
RefSeq Size 4045 bp
RefSeq ORF 2756 bp
Locus ID 15275
Gene Summary Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine (By similarity). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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