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Brd8 (BC025644) Mouse Tagged ORF Clone
CAT＃: MG211186
- TrueORF®
Brd8 (tGFP-tagged) - Mouse bromodomain containing 8 (cDNA clone MGC:36597 IMAGE:5323755)
ORF Plasmid: DDK
"BC025644" in other vectors (3)
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CNY 5,800.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SMAP, 4933408B17Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211186 representing BC025644
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGTGGGGATCAGAACTGGGTATCAGTTAGCAGAGCAATCAAGCCCTTTGCAGAACCTGGCCGGC CTCCAGACTGGTTCTCTCAAAAACATTGTGCTTCTCAGTATTCTGAGCTCTTAGAGACTACTGAGACTCC AAAACGGAAACGGGGTGAAAAAGGAGAAGTGGTAGAAACTGTTGAAGATGTTATTGTTCGCAAACTGACT GCTGAGAGAGTTGAAGAACTGAAGAAAGTCATAAAGGAGACACAGGAGAGATACAGGCGTCTGAAAAGAG ATGCAGAACTAATCCAAGCCGGGCACATGGACAGCAGACTGGATGAGCTCTGCAATGACATTGCAATGAA AAAGAAATTGGAAGAGGAAGAGGCTGAAGTAAAAAGGAAGGCCACCGATGCTGCGTACCAGGCGCGACAA GCAGTGAAAACGCCTCCTCGAAGGTTACCGACTGTGATGGTCCGCTCTCCTGTAGACTCTGCCTCCCCAG GAGGTGATTACCCACTTGGAGACTTGACTCCAACCACTATGGAAGAGGCTACCTCTGGGGTAACCCCTGG GACTTTGCCGAGTACCCCAGTCACCTCGTTTCCTGGGATTCCTGACACCCTTCCTCCAGGCTCTGCACCC TTAGAAGCCCCCATGACCCCAATAACAGATGATTCACCCCAGAAAAAGATGCTTGGACAGAAAGCAACTC CACCCCCCTCCCCTCTGCTGTCAGAGCTCTTGAAGAAGGGCAGCCTCCTGCCTACTAGCCCCAGACTGGT GAATGAGAGTGAAATGCCTGTACCCCCTGGCCATCTGAACAGCACAGGGGTTCTCTTGGAGGTAGGAGGG GTTCTTCCCATGATACATGGTGGGGAGATACAGCCAACAACCAGTGCTGTGGCGGCCTCCCCGGCTGCCT CAGGTGCTCCCACTCTTTCCCGGCTTTTAGAAGCTGGGCCCACACAGTTCACCACTCCTCTTCCTTCCTT CACTACTGTTGCCAGTGAGCCGCCAGTTAAGCTTGTGCCACCCCCTGTAGAGTCTGTGTCCCAGGCTACC ATTGTCATGATGCCCGCGCTGCCAGCACCATCCTCTGCTGCGGCTGTCTCCACTTCTGAAAGTGGAGCTC CAGTGAGCCAGCCTGAGCCCTGTGTACCCCTGGAAGCTGTGGGGGATCCACATACTGTGACTGTTTCCAT GGATAGCAATGAAATCTCCATGATCATTAATTCCATCAAAGAAGAGTGTTTCCGCTCAGGGGTAGCAGAG GCTCCTGGTGGATCAAAGGCTCCAAGCATAGATGGGAAGGAAGACTTGGATCTAGCGGAGAAGATGGATA TTGCTGTGTCTTACACAGGTGAAGAGTTGGACTTTGAAACAGTTGGAGACATCATTGCCATCATTGAGGA CAAGGTGGATGATCACCCTGAAGTGCTGGATGTGGCAGCGGTAGAAGCGGCGTTATCGTTCTGTGAAGAG AATGATGACCCTCAGTCCCTGCCTGGCCCCTGGGAGCACCCTATCCAGCAGGAGCGCGACAAGCCAGTAC CTCTGCCAGCACCAGAGATGACAGTCAAACAAGAGAGGCTAGACTTCGAGGAATCAGAAAACAAAGGGCT CCATGACCTGGTGGACATCAGGGATTCCGGTGTTGAGATTAAGGTGGAACCCACAGAGCCAGAGCCAGGC ATGTCTGGGGCTGAGATAGTAGCTGGAGTTGGTCCAGTTCCAAGTATGGAGCCACCAGAACTCAGGAGTC AAGACTCAGATGAAGAACCTAGAAGTTCTGCAGCTGGAGACATTGGTGAGGCAGATGGTTCCAGTGGGAA AGGCGATGAGAGGCCACTTTCAGCTGTGAAGACAGAGGCATCCCCTGAGAGCATGTTGTCTCCATCACAT GGCTCAAATCTTATTGAAGATCCTTTAGAGGCAGAGACTCAACACAAGTTTGAAATGTCAGACTCATTGA AAGAAGAATCAGGGACTATTTTTGGAAGCCAGATAAAGGATGCCCCAGGTGACGATGAGGAAGAAGATGG AGTCAGTGAAGCAGCTAGCCTAGAGGAGCCTAAGGAAGAGGATCAAGGAGAAGGCTATTTGTCTGAGATG GATAATGAGCCCCCTGTGAGTGAGAGTGATGATGGCTTTAGTATACATAACGCCACACTGCAGTCACACA CTCTGGCAGACTCCATCCCAAGCAGCCCTGCCTCCTCCCAGTTTTCCGTGTGCAGTGAAGATCAAGAAGC AATTCAGGCTCAGAAAATATGGAAGAAAGCCATCATGCTTGTATGGAGGGCTGCAGCAAATCATAGGTAT GCCAATGTGTTCCTGCAACCTGTTACAGATGACATAGCTCCTGGTTACCATAGCATTGTACAGAGGCCTA TGGATTTGTCAACTATAAAGAAAAACATTGAAAATGGACTGATCCGAAGCACAGCTGAGTTTCAGCGTGA CATCATGCTGATGTTTCAGAATGCTGTTATGTACAATAGCTCAGACCATGATGTCTATCACATGGCAGTA GAGATGCAGAGAGATGTCTTGGAACAGATCCAGCAATTCTTGGCCACACAGTTGATTATGCAAACATCTG AGTCTGGAATCAGTGCTAAAAGTCTCCGGGGGAGAGACTCTACCCGAAAACAAGATGCTTCAGAGAAGGA CAGTGTCCCCATGGGCTCTCCTGCCTTCCTTCTCTCTCTCTTTGATGGGGGAACCAGGGGACGCCGCTGT GCCATTGAAGCAGATATGAAGATGAAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211186 representing BC025644
Red=Cloning site Green=Tags(s) MRSGDQNWVSVSRAIKPFAEPGRPPDWFSQKHCASQYSELLETTETPKRKRGEKGEVVETVEDVIVRKLT AERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDELCNDIAMKKKLEEEEAEVKRKATDAAYQARQ AVKTPPRRLPTVMVRSPVDSASPGGDYPLGDLTPTTMEEATSGVTPGTLPSTPVTSFPGIPDTLPPGSAP LEAPMTPITDDSPQKKMLGQKATPPPSPLLSELLKKGSLLPTSPRLVNESEMPVPPGHLNSTGVLLEVGG VLPMIHGGEIQPTTSAVAASPAASGAPTLSRLLEAGPTQFTTPLPSFTTVASEPPVKLVPPPVESVSQAT IVMMPALPAPSSAAAVSTSESGAPVSQPEPCVPLEAVGDPHTVTVSMDSNEISMIINSIKEECFRSGVAE APGGSKAPSIDGKEDLDLAEKMDIAVSYTGEELDFETVGDIIAIIEDKVDDHPEVLDVAAVEAALSFCEE NDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDFEESENKGLHDLVDIRDSGVEIKVEPTEPEPG MSGAEIVAGVGPVPSMEPPELRSQDSDEEPRSSAAGDIGEADGSSGKGDERPLSAVKTEASPESMLSPSH GSNLIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAPGDDEEEDGVSEAASLEEPKEEDQGEGYLSEM DNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFSVCSEDQEAIQAQKIWKKAIMLVWRAAANHRY ANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIRSTAEFQRDIMLMFQNAVMYNSSDHDVYHMAV EMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTRKQDASEKDSVPMGSPAFLLSLFDGGTRGRRC AIEADMKMKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC025644 |
| ORF Size | 2762 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC025644, AAH25644 |
| RefSeq Size | 3277 bp |
| RefSeq ORF | 2762 bp |
| Locus ID | 78656 |
| Gene Summary | May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR211186 | Brd8 (Myc-DDK-tagged) - Mouse bromodomain containing 8 (cDNA clone MGC:36597 IMAGE:5323755) |
CNY 6,736.00 |
|
| MR211186L3 | Lenti ORF clone of Brd8 (Myc-DDK-tagged) - Mouse bromodomain containing 8 (cDNA clone MGC:36597 IMAGE:5323755) |
CNY 7,130.00 |
|
| MR211186L4 | Lenti ORF clone of Brd8 (mGFP-tagged) - Mouse bromodomain containing 8 (cDNA clone MGC:36597 IMAGE:5323755) |
CNY 7,130.00 |
