Gnptab (BC060638) Mouse Tagged ORF Clone

CAT#: MG211191

  • TrueORF®

Gnptab (tGFP-tagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (cDNA clone MGC:79224 IMAGE:6849405), complete

ORF Plasmid: DDK tGFP



  "BC060638" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 10,170.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms EG432486; mKIAA1208
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211191 representing BC060638
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGAAACCCTCGGGAAGAACACAACCGAACCGACAAAGAAGAGTGAGAAGCAGCTGGAATGTCTGC
TGACGCACTGCATTAAGGTGCCCATGCTTGTTCTGGACCCGCCCCTGCCAGCCAACTGCACCCTGAAGGA
TCTGCCAACCCTTTATCCATCTTTCCACGCTGCCAGCGACATGTTCAATGTTGCGAAACCAAAAAATCCG
TCTACTAATGTCTCCGTTGTCGTTTTTGACACTACTAAGGATGTTGAAGACGCCCATGCTGGACCGTTTA
AGGGAGGCAGCAAACAGATGGTTTGGAGAGCCTACTTGACAACAGACAAAGAAGCCCCTGGCTTAGTGCT
GATGCAAGGCTTGGCGTTCCTGAGTGGATTCCCACCGACCTTCAAGGAGACGAGTCAACTGAAGACAAAG
CTGCCAGAAAAGCTTTCCTCTAAAATAAAGCTGTTGCGGCTGTACTCGGAGGCCAGTGTCGCTCTTCTGA
AATTGAATAATCCCAAGGGTTTCCAAGAGCTGAACAAGCAGACCAAGAAGAACATGACCATCGATGGGAA
GGAACTGACCATCAGCCCTGCGTATCTGCTGTGGGACCTGAGTGCCATCAGCCAGTCCAAGCAGGATGAG
GACGTGTCTGCCAGCCGCTTTGAGGATAATGAAGAGCTGAGGTACTCGCTGCGATCTATCGAGAGACACG
CGCCATGGGTACGGAATATTTTCATTGTCACCAACGGGCAGATTCCATCCTGGCTGAACCTTGACAACCC
TCGAGTGACCATAGTGACCCACCAGGACATTTTCCAAAATCTGAGCCACTTGCCTACTTTCAGTTCCCCT
GCTATTGAAAGTCACATTCACCGCATCGAAGGGCTGTCCCAGAAGTTTATTTATCTAAATGACGATGTCA
TGTTCGGTAAGGACGTCTGGCCGGACGATTTTTACAGCCACTCCAAAGGTCAAAAGGTTTATTTGACATG
GCCTGTGCCAAACTGTGCAGAGGGCTGCCCGGGCTCCTGGATTAAAGACGGCTATTGTGATAAGGCCTGT
AATAACTCAGCCTGTGACTGGGATGGCGGAGACTGCTCTGGTAATACTGCAGGGAACCGGTTTGTTGCAG
GAGGTGGGGGTACCGGGAATATTGGAGCTGGACAGCACTGGCAGTTTGGTGGAGGAATAAACACCATCTC
TTACTGTAACCAAGGATGTGCAAACTCCTGGCTGGCTGACAAGTTCTGTGACCAAGCCTGTAACGTCTTA
TCCTGCGGGTTTGATGCTGGTGACTGTGGACAAGATCATTTTCATGAATTGTATAAAGTAACACTTCTCC
CAAACCAGACTCACTATGTTGTCCCCAAAGGTGAATACCTGTCTTATTTCAGCTTTGCTGGTGAACACAG
ATCAGAGAGATGGACTGCCCCAGCAGAGACAGTGACCGTGAAAGGCCGTGACCACGCTTTGAATCCACCC
CCGGTGTTGGAGACCAATGCAAGATTGGCCCAGCCTACACTAGGCGTGACTGTGTCCAAAGAGAACCTTT
CACCGCTGATCGTTCCCCCAGAAAGCCACTTGCCAAAAGAAGAGGAGAGTGACAGGGCAGAAGGCAATGC
TGTACCTGTAAAGGAGTTAGTGCCTGGCAGACGGTTGCAGCAGAATTATCCAGGCTTTTTGCCCTGGGAG
AAAAAAAAGTATTTCCAAGACCTTCTTGATGAGGAAGAGTCATTGAAGACCCAGTTGGCGTACTTTACAG
ACAGCAAACATACCGGGAGGCAACTAAAAGATACATTTGCAGACTCCCTCCGATACGTCAATAAAATTCT
CAACAGCAAGTTTGGATTCACATCCAGGAAAGTCCCTGCACACATGCCGCACATGATTGACAGGATCGTT
ATGCAAGAACTCCAAGATATGTTCCCTGAAGAATTTGACAAGACTTCATTTCACAAGGTGCGTCACTCTG
AGGACATGCAGTTTGCCTTCTCCTACTTTTATTACCTCATGAGTGCAGTTCAGCCCCTCAATATTTCCCA
AGTCTTTCATGAAGTAGACACAGACCAATCTGGTGTCTTGTCTGATAGGGAAATCCGAACACTGGCCACG
AGAATTCACGACCTACCTTTAAGCTTGCAGGATTTGACAGGTTTGGAACACATGTTAATAAATTGCTCAA
AAATGCTCCCCGCTAATATCACTCAACTCAACAACATCCCACCGACTCAGGAAGCATACTACGACCCCAA
CCTGCCTCCGGTCACTAAGAGTCTTGTCACCAACTGTAAGCCAGTAACTGACAAGATCCACAAAGCCTAT
AAAGACAAGAACAAATACAGGTTTGAAATCATGGGAGAGGAAGAAATCGCTTTCAAGATGATACGAACCA
ATGTTTCTCATGTGGTTGGCCAGTTGGATGACATCAGAAAAAACCCCAGGAAGTTCGTTTGTCTGAATGA
CAACATTGACCACAACCATAAAGATGCCCGGACAGTGAAGGCTGTCCTCAGGGACTTCTATGAGTCCATG
TTTCCCATACCTTCCCAGTTTGAGCTGCCAAGAGAGTATCGGAACCGCTTTCTGCACATGCATGAGCTCC
AAGAATGGCGGGCATATCGAGACAAGCTGAAGTTTTGGACCCACTGCGTACTAGCAACGTTGATTATATT
TACTATATTCTCATTTTTTGCTGAACAGATAATTGCTCTGAAGCGAAAGATATTTCCCAGGAGGAGGATA
CACAAAGAAGCTAGTCCAGACCGAATCAGGGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211191 representing BC060638
Red=Cloning site Green=Tags(s)

MRETLGKNTTEPTKKSEKQLECLLTHCIKVPMLVLDPPLPANCTLKDLPTLYPSFHAASDMFNVAKPKNP
STNVSVVVFDTTKDVEDAHAGPFKGGSKQMVWRAYLTTDKEAPGLVLMQGLAFLSGFPPTFKETSQLKTK
LPEKLSSKIKLLRLYSEASVALLKLNNPKGFQELNKQTKKNMTIDGKELTISPAYLLWDLSAISQSKQDE
DVSASRFEDNEELRYSLRSIERHAPWVRNIFIVTNGQIPSWLNLDNPRVTIVTHQDIFQNLSHLPTFSSP
AIESHIHRIEGLSQKFIYLNDDVMFGKDVWPDDFYSHSKGQKVYLTWPVPNCAEGCPGSWIKDGYCDKAC
NNSACDWDGGDCSGNTAGNRFVAGGGGTGNIGAGQHWQFGGGINTISYCNQGCANSWLADKFCDQACNVL
SCGFDAGDCGQDHFHELYKVTLLPNQTHYVVPKGEYLSYFSFAGEHRSERWTAPAETVTVKGRDHALNPP
PVLETNARLAQPTLGVTVSKENLSPLIVPPESHLPKEEESDRAEGNAVPVKELVPGRRLQQNYPGFLPWE
KKKYFQDLLDEEESLKTQLAYFTDSKHTGRQLKDTFADSLRYVNKILNSKFGFTSRKVPAHMPHMIDRIV
MQELQDMFPEEFDKTSFHKVRHSEDMQFAFSYFYYLMSAVQPLNISQVFHEVDTDQSGVLSDREIRTLAT
RIHDLPLSLQDLTGLEHMLINCSKMLPANITQLNNIPPTQEAYYDPNLPPVTKSLVTNCKPVTDKIHKAY
KDKNKYRFEIMGEEEIAFKMIRTNVSHVVGQLDDIRKNPRKFVCLNDNIDHNHKDARTVKAVLRDFYESM
FPIPSQFELPREYRNRFLHMHELQEWRAYRDKLKFWTHCVLATLIIFTIFSFFAEQIIALKRKIFPRRRI
HKEASPDRIRV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC060638
ORF Size 2763 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060638.1
RefSeq Size 4699 bp
RefSeq ORF 2765 bp
Locus ID 432486
Gene Summary Catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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