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Aass (NM_013930) Mouse Tagged ORF Clone
CAT＃: MG211209
- TrueORF®
Aass (tGFP-tagged) - Mouse aminoadipate-semialdehyde synthase (Aass), nuclear gene encoding mitochondrial protein
ORF Plasmid: DDK
"NM_013930" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | LKR; LKR/SDH; Lo; LOR; LOR/SDH; Lorsdh; SDH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211209 representing NM_013930
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGAGCGCAGAGGCCGAGACTGGCCAGGCTTAGAGCCTGCCTCTCCAGAGGCCTCCACCACAAGC CAGTGATGGCACTCCGGAGGGAGGACGTGAACGCCTGGGAGCGCAGGGCGCCTCTGGCTCCCAAGCACAT CAAGGGCATCACCAAACTGGGCTACAAGGTCTTGATACAGCCTTCCAACAGACGGGCCATCCATGACAAG GAGTATGTCAGGGCTGGTGGCATTCTTCAGGAAGACATCACAGAAGCCTGTCTGATTTTGGGAGTAAAAA GGCCTCCAGAGGAAAAACTGATGTCCAAGAAGACGTACGCCTTCTTTTCCCACACAATAAAAGCTCAGGA AGCCAATATGAACCTGTTGGATGAGGTTCTGAAACAGGAAATCCGGCTCATTGATTATGAGAAAATGGTG GATCACAGAGGGTCTCGGATAGTGGCTTTCGGACAGTGGGCGGGTGTGGCAGGAATGATTAATATTTTAC ATGGAATGGGCTTAAGGCTCCTTGCTTTGGGACATCATACGCCTTTTATGCATCTTGGCATGGCTCACAA CTACAGGAACAGCAGCCAGGCCGTGCAGGCTGTCCGTGATGCTGGCTATGAAATATCTCTGGGTTTAATG CCAAAGTCAATAGGACCCTTAACGTTTGTCTTCACAGGGACTGGCAATGTATCCAAGGGAGCCCAGGAAG TCTTTAATGAGCTACCTTGTGAGTATGTGGAGCCCCACGAATTAAGAGAAGTTTCAAAAACTGGAGACCT CAGGAAAGTGTATGGGACGGTGTTAAGTCGCCATCATCATCTTGTCAGGAAAACAGATGGTGTGTACGAT CCCGTAGAATACGAGAAATATCCTGAGCGCTACACAAGTCGTTTTAATACAGATATTGCGCCCTATACAA CTTGTTTAATTAATGGAATCTACTGGGAGCAAAACACCCCTCGCCTGCTCACACGTCAGGATGCTCAGAG TCTCCTGGTCCCAGTGAAGTCCTCAGTTGTTCCTGTTGAAGGATGTCCTGAATTACCACACAAACTTGTG GCAATATGTGACATTTCAGCAGACACGGGAGGGTCCATAGATTTTATGACTGAGTGCACCACCATAGAAC GGCCCTTTTGCATGTATGACGCAGACCAGCAAATTATTCACGACAGTGTTGAAGGCTCCGGGATCCTGAT GTGTTCCATTGACAATTTGCCAGCACAGCTTCCAATCGAGGCTACGGAATACTTTGGAGACATGCTTTAC CCTTATGTTGAAGAAATGTTGTTATCCGATGCATCACAACCTCTGGAAAGCCAGAACTTCTCCCCAGTGG TGCGAGACGCAGTGATTACGTCCAATGGTCTATTGACTGACAAATACAAATATATACAGAAACTCCGAGA AAGCAGAGAACGCATTCAATTCCTTTCAATGAGTACCAAGAAGAAGGTTTTGGTCCTTGGGTCTGGCTAT GTATCTGGGCCTGTTTTGGAATATCTGTCAAGAGACAACAATATAGAAATAACACTAGGCTCTGACATGA CCAACCAAATGCAGCAGCTGAGCAAGAAATACAACATTAACCCTGTCAGTTTGACTGTTGGCAAGCAAGA GGCAAAGCTGCAATCCCTGGTGGAATCCCAGGATCTTGTGATCAGCTTGTTGCCTTATGTGCTTCATCCT GTGGTGGCCAAAGCCTGCATCGAAAGCAGGGTTAATATGGTCACTGCAAGCTATATTACACCAGCCATGA AAGAACTGGAAAAGAGTGTGGATGATGCTGGCATTACAGTCATTGGTGAGTTGGGATTAGACCCTGGTCT TGATCACATGTTGGCAATGGAAACAATTGATACTGCCAAAGAGCTGGGAGCCACGGTTGAGTCTTATGTT TCCTACTGTGGTGGCCTTCCGGCCCCTGAACATTCAGATAATCCCTTGCGATACAAATTCAGCTGGAGTC CAGTGGGAGTCTTGATGAACATAATGCAGCCTGCTAGCTATCTGCTCAATGGGAAGGTTGTGAATGTGAC AGGGGGAGTCTCTTTTCTCAACTCTGTCACACCCATGGATTACTTCCCAGGTCTAAACTTGGAAGGCTAT CCTAACAGAGATAGCATAAAATATGCTGAGATTTATGGAATTTCATCTGCCCATACATTATTAAGGGGAA CACTGAGATATAAGGGATATTCCAAAGCCTTGAATGGATTTGTGAAGTTGGGACTCATCAACAGAGAAGC ATACCCTGCCCTCCGACCTGAGGCCAACCCTCTCACCTGGAAACAACTCCTATGTGACCTAGTTGGAATT TCCCGCTCCTCCCCATGCGAGAAGCTTAAGGAAGTTGTCTTCACAAAGCTGGGAGGGGACAATACCCAGC TGGAAGCTGCAGAATGGTTGGGCTTACTGGGGGATGAACAAGTTCCTCAGGCGGAGTCCATTGTGGACGC GTTCTCCAAGCACTTGGTCTCAAAACTCTCCTATGGCCCTGAAGAAAAGGATATGATTGTGATGAGGGAC AGCTTTGGCATCAGACATCCATCTGGACATTTAGAAAATAAGACCATTGATCTCGTGGTTTATGGAGACT TCAACGGCTTCTCGGCAATGGCTAAAACTGTGGGGTTGCCGACAGCCATGGCAGCCAAAATGTTGCTTGA TGGTGAAATTGAAGCCAAAGGCCTAATGGGGCCCTTTACAAAGGAGATCTATGGGCCAATATTGGAGCGG ATTAAAGCAGAAGGCATTGTATTTAACACACAGAGTACAATCAAGCTA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211209 representing NM_013930
Red=Cloning site Green=Tags(s) MLRAQRPRLARLRACLSRGLHHKPVMALRREDVNAWERRAPLAPKHIKGITKLGYKVLIQPSNRRAIHDK EYVRAGGILQEDITEACLILGVKRPPEEKLMSKKTYAFFSHTIKAQEANMNLLDEVLKQEIRLIDYEKMV DHRGSRIVAFGQWAGVAGMINILHGMGLRLLALGHHTPFMHLGMAHNYRNSSQAVQAVRDAGYEISLGLM PKSIGPLTFVFTGTGNVSKGAQEVFNELPCEYVEPHELREVSKTGDLRKVYGTVLSRHHHLVRKTDGVYD PVEYEKYPERYTSRFNTDIAPYTTCLINGIYWEQNTPRLLTRQDAQSLLVPVKSSVVPVEGCPELPHKLV AICDISADTGGSIDFMTECTTIERPFCMYDADQQIIHDSVEGSGILMCSIDNLPAQLPIEATEYFGDMLY PYVEEMLLSDASQPLESQNFSPVVRDAVITSNGLLTDKYKYIQKLRESRERIQFLSMSTKKKVLVLGSGY VSGPVLEYLSRDNNIEITLGSDMTNQMQQLSKKYNINPVSLTVGKQEAKLQSLVESQDLVISLLPYVLHP VVAKACIESRVNMVTASYITPAMKELEKSVDDAGITVIGELGLDPGLDHMLAMETIDTAKELGATVESYV SYCGGLPAPEHSDNPLRYKFSWSPVGVLMNIMQPASYLLNGKVVNVTGGVSFLNSVTPMDYFPGLNLEGY PNRDSIKYAEIYGISSAHTLLRGTLRYKGYSKALNGFVKLGLINREAYPALRPEANPLTWKQLLCDLVGI SRSSPCEKLKEVVFTKLGGDNTQLEAAEWLGLLGDEQVPQAESIVDAFSKHLVSKLSYGPEEKDMIVMRD SFGIRHPSGHLENKTIDLVVYGDFNGFSAMAKTVGLPTAMAAKMLLDGEIEAKGLMGPFTKEIYGPILER IKAEGIVFNTQSTIKL SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013930 |
| ORF Size | 2778 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013930.4, NP_038958.2 |
| RefSeq Size | 3701 bp |
| RefSeq ORF | 2781 bp |
| Locus ID | 30956 |
| UniProt ID | Q99K67 |
| Gene Summary | This gene encodes a bifunctional mitochondrial protein that catalyzes the first two steps in the lysine degradation pathway. The N-terminus contains lysine-ketoglutarate reductase activity and converts lysine to saccharopine, whereas the C-terminus contains saccharopine dehydrogenase activity and converts saccharopine to alpha-aminoadipate semialdehyde. Mutations in a human gene encoding a highly similar protein are associated with familial hyperlysinemia. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205647 | Aass (untagged) - Mouse aminoadipate-semialdehyde synthase (Aass), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 6,792.00 |
|
| MR211209 | Aass (Myc-DDK-tagged) - Mouse aminoadipate-semialdehyde synthase (Aass), nuclear gene encoding mitochondrial protein |
CNY 7,464.00 |
|
| MR211209L3 | Lenti ORF clone of Aass (Myc-DDK-tagged) - Mouse aminoadipate-semialdehyde synthase (Aass), nuclear gene encoding mitochondrial protein |
CNY 9,030.00 |
|
| MR211209L4 | Lenti ORF clone of Aass (mGFP-tagged) - Mouse aminoadipate-semialdehyde synthase (Aass), nuclear gene encoding mitochondrial protein |
CNY 9,030.00 |
