Unc45b (BC084585) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AA445617; Cmya4; D230041A13Rik; Unc45
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211221 representing BC084585 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGGCTGAAGCGGCACAGCTGAAGGAGGAGGGGAACCGGCATTTTCAGCTCCAGGATTACAAGG CGGCCACCAAGAGCTACAGCCAGGCCCTGAAGCTGACCAAGGACAAGGCTCTGCTAGCTACACTCTACCG GAATCGGGCAGCCTGCGGCCTGAAAATGGAGAGCTACGCCCAGGCAGCCTCGGATGCTTCCAGAGCCATT GACATCAACTCTGCTGACATCAAAGCATTGTATCGGCGATGCCAGGCACTGGAGCATCTTGGCAAGTTGG ACCAGGCATTCAAGGATGTGCAGCGCTGTGCTACCCTGGAGCCAAGGAACCAGAACTTCCAGGAGACACT GCGGAGGCTCAACACCAGCATCCAAGAGCAGCTCCGTGTGCAGTTTTCTACAGACTCCAGGGTACAGACG ATGTTTGAAATTCTTCTGAACGAAAACAGCGAGGCTGATAAGCGAGAGAAGGCTGCCAACAACCTCATTG TCCTGGGCCGTGAAGAAGCGGGTGCTGAGAGGATCCTCCAGAGCAATGGAGTGGCTCTGCTGCTGCAGCT TATGAACACCCAGCGGCCTGAGCTGCTGCTAGCGGCTGTGCGGACGCTGTCTGGCATGTGCAGTGGCCAC AGAGCTAGGGCCACCGCCATCCTGCACGCAGTCCGCATAGACAGAATCTGCAGCCTCATGGCCCTGGAGA ATGAAGAGATGTCCCTGGCTGTCTGCAACCTGCTCCAGGCCATCATTGACTCCCTGTCTGGAGAGGACAA GAGAGAGCATCGTGGGAAGGAGGAGGCCTTGGTTCTAGATACCAAGAAGGACCTGAAGCAGATCACCAGC CACCTGCTGGACATGCTGGTCAGTAAGAAAGTGTCTGGGCAGGGCAGAGACCAGGCACTGAACCTGCTTA ATAAGAATGTCCCCAGGAAGGACCTGTCCATTCATGACAACTCACGCACCATCTATGTTGTAGATAACGG TCTGAGGAAGATCCTGAAGGTGGTGGGGCAGGTTCCAGACCTGCCCTCCTGCCTACCCCTGACGGACAAC ACCCGCATGCTGGCCTCCATCCTCATCAACAAGCTCTATGATGACCTGCGCTGTGACCCAGAGCGCGACC ACTTCCGCAAGATCTGTGAGGAGTATATCACGAGCAAATTTGACCCCCAGGACATGGACAAGAATGTAAA TGCCATCCAGACGGTGTCCGGCATCTTGCAGGGGCCCTTTGACCTGGGCAATCAGCTGCTGGGCATGAAA GGTGTGATGGAGATGATGGTGGCTCTGTGTGGCTCGGAGCGCGAGGCAGACCAGCTGGTGGCTGTGGAGG CTCTCATCCACGCCTCCACGAAGCTCAGCCGGGCCACCTTCATCATCACCAACGGAGTGACACTTCTTAA GCAGATCTACAAGACCACAAAGAATGAGAAGATCAAGATCCGCACGCTGGTGGGGCTCTGTAAACTTGGC TCAGCTGGAGGCTCCGACTATGGTCTCAGACAGTTTGCTGAAGGGTCAACAGAGAAGCTAGCCAAGCAGT GTCGAAAGTGGCTGTGTAACACCGCCATAGACACCCGGACGCGGCGCTGGGCAGTGGAGGGCCTGGCCTA CCTCACGCTGGACGCTGACGTGAAGGACGACTTTGTCCAGGACATCCCTGCTCTGCAGGCCATGTTTGAG TTGGCCAAGACCAGTGACAAGACCATCCTCTACTCAGTGGCCAACACCCTGGTAAACTGCACCAACAGCT ATGATGTCAAGGAGGTCGTCCCTGAGCTGGTGCAGCTGGCCAAGTTCTCCAAGCAACATGTGCCAGAGGA GCACCCCAAGGACAAGAAGGACTTCGTTGACTTGCGGGTGAAACGTCTTCTGAAGGCAGGTGTCATATCT GCACTGGCCTGCATGGTGAAGGCAGACAGTGCCATCCTCACAGACCAGACCAAGGAGCTGCTGGCCAGGG TATTCCTGGCACTCTGTGACAATCCAAAGGACCGGGGCACCATTGTAGCTCAAGGTGGTGGCAAGGCCCT GATCCCCCTGGCTTTGGAGGGCACAGATGTGGGCAAGGTGAAGGCAGCCCATGGGCTAGCCAAGATTGCT GCTGTCTCCAACCCGGACATTGCCTTCCCTGGGGAGAGGGTATATGAGGTGGTGCGGCCCCTCGTGAGTC TCCTGGACACACAGAGGGATGGGCTTCAGAACTATGAGGCTCTGCTGGGACTGACTAACCTGTCTGGGCG CAGTGACAAACTCAGGCAGAAGATCTTCAAGGAGAAGGCCTTGCCAGACATCGAAAACTACATGTTTGAG AACCATGACCAGCTACGGCAGGCAGCCACCGAATGCATGTGTAACATGGTGCTCAATAAGGAGGTTCAGG AGAGGTTCCTGGCCGATGGGAATGATCGTCTGAAGCTGGTGGTGCTGCTCTGTGGGGAGGATGACCACAA ACTGCAGAACGCAGCAGCTGGAGCTCTAGCCATGCTGACCGCGGCACATAAGAAACTGTGCCTCAAGATG ACGGAGGTGACAACCCAGTGGCTGGAGATCCTACAGCGGCTTTGCCTGCATGACCAGTTGTCCGTGCAGC ACCGGGGCCTGGTCATTGCCCACAACCTGCTATCAGCGGATGCTGAGCTGGCGAGGAAGCTGGTGGAGAG CGAGCTGCTGGAGATCCTGACTGTGGTGGGAAAGCAGGAGCCAGATGAGAAGAGAGCGGCTGTGGTCCAG ACAGCCCGGGAGTGTCTTATCAAGTGCATGGATTATGGCTTCATCAAGCCGGTGTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211221 representing BC084585 Red=Cloning site Green=Tags(s) MAEAEAAQLKEEGNRHFQLQDYKAATKSYSQALKLTKDKALLATLYRNRAACGLKMESYAQAASDASRAI DINSADIKALYRRCQALEHLGKLDQAFKDVQRCATLEPRNQNFQETLRRLNTSIQEQLRVQFSTDSRVQT MFEILLNENSEADKREKAANNLIVLGREEAGAERILQSNGVALLLQLMNTQRPELLLAAVRTLSGMCSGH RARATAILHAVRIDRICSLMALENEEMSLAVCNLLQAIIDSLSGEDKREHRGKEEALVLDTKKDLKQITS HLLDMLVSKKVSGQGRDQALNLLNKNVPRKDLSIHDNSRTIYVVDNGLRKILKVVGQVPDLPSCLPLTDN TRMLASILINKLYDDLRCDPERDHFRKICEEYITSKFDPQDMDKNVNAIQTVSGILQGPFDLGNQLLGMK GVMEMMVALCGSEREADQLVAVEALIHASTKLSRATFIITNGVTLLKQIYKTTKNEKIKIRTLVGLCKLG SAGGSDYGLRQFAEGSTEKLAKQCRKWLCNTAIDTRTRRWAVEGLAYLTLDADVKDDFVQDIPALQAMFE LAKTSDKTILYSVANTLVNCTNSYDVKEVVPELVQLAKFSKQHVPEEHPKDKKDFVDLRVKRLLKAGVIS ALACMVKADSAILTDQTKELLARVFLALCDNPKDRGTIVAQGGGKALIPLALEGTDVGKVKAAHGLAKIA AVSNPDIAFPGERVYEVVRPLVSLLDTQRDGLQNYEALLGLTNLSGRSDKLRQKIFKEKALPDIENYMFE NHDQLRQAATECMCNMVLNKEVQERFLADGNDRLKLVVLLCGEDDHKLQNAAAGALAMLTAAHKKLCLKM TEVTTQWLEILQRLCLHDQLSVQHRGLVIAHNLLSADAELARKLVESELLEILTVVGKQEPDEKRAAVVQ TARECLIKCMDYGFIKPVS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC084585
ORF Size	2789 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC084585, AAH84585
RefSeq Size	3610 bp
RefSeq ORF	2789 bp
Locus ID	217012
Gene Summary	Acts as a co-chaperone for HSP90 and is required for proper folding of the myosin motor domain. Plays a role in sarcomere formation during muscle cell development (PubMed:12356907, PubMed:18326487, PubMed:18478096). Is necessary for normal early lens development (By similarity).[UniProtKB/Swiss-Prot Function]

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