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Pkn1 (NM_177262) Mouse Tagged ORF Clone
CAT＃: MG211277
- TrueORF®
Pkn1 (tGFP-tagged) - Mouse protein kinase N1 (Pkn1)
ORF Plasmid: DDK
"NM_177262" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DBK; F730027O18Rik; PAK1; Pkn; PRK1; Prkcl1; Stk3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211277 representing NM_177262
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCGACGCCGTACAGAGTGAGCCTCGAAGCTGGTCGCTGCTGGAACAGCTGGGTCTGGCTGGGG CTGACCTGGCAGCCCCCGGGGTGCAGCAACAGCTGGAGTTAGAGCGAGAGCGACTGAAGCGGGAGATACG GAAGGAGCTGAAGCTGAAGGAGGGCGCTGAGAACCTGAGGCGAGCCACCACTGACCTGGGCCGTAGCCTG GCCCCTGTGGAGCTGCTGCTGCGGGGCTCCGCTCGACGCCTAGACTTACTGCACCAGCAGCTGCAGGAGC TGCATGCACATGTGGTGCTGCCTGACCCTGCAGCTGGAAGCGATGCTACCCAATCCCTTGCAGAGGGCAG CCCTATCTGCTCATCCACCAACCTGAGCAGAGTGGCTGGGCTGGAGAAGCAGCTGGCCATTGAGCTCAAG GTCAAACAGGGGGCAGAGAACATGATCCAGACCTATAGCAATGGCAGCAGCAAGGACCGGAAGCTGTTGT TGACAGCCCAGCAGATGCTGCAGGACAGTAAGACCAAGATTGACATCATCCGCATGCAGCTTCGAAGAGC GCTCCAGGCACTACAGGCTGGAGAGCTGGAGAGTCAGGCAGCTCCTGATGAAGCCCAAGGAGATCCAGAA CTGGGAGCCGTAGAGCTACGCATTGAGGAGCTACGACACCATTTTCGAGTGGAGCATGCAGTGGCAGAAG GTGCCAAGAATGTCCTGCGCCTGCTCAGTGGGGCAAAGGCCCCAGACCGCAAGGCAGTCAGCGAGGCTCA GGAGAAATTGACTGAGTCCAACCAGAAGCTGGGCTTGCTGCGGGAATCACTGGAGAGGCGCCTTGGGGAG TTGCCTGCCGATCACCCCAAGGGGCGGCTGCTTCGGGAGGAGCTCACTGCAGCCTCGTCCTCAGCCTTCA GCGCCATACTGCCTGGGCCCTTCCCTGCCACTCACTACAGCACCTTGAGCAAGCCTGCACCGCTCACAGG GACCCTGGAAGTACGAGTGGTGGGCTGCAAAAACCTTCCCGAGACCATCCCCTGGAGCCCTCCCCCCTCA GTGGGGGCATCTGGGACCCCCGAAAGCCGCACTCCGTTCCTGAGTCGCCCCGCTCGGGGCCTTTACAGCC GGAGTGGAAGCCTTAGTGGACGGAGCAGCCTCAGAGGGGAGGCAGAGAATGCCACTGAGGTCAGCACCGT GCTCAAGCTGGACAACACAGTGGTGGGGCAAACAGCCTGGAAGCCATGCGGCCCCAATGCCTGGGACCAG AGCTTTACCCTGGAGCTGGAGAGGGCTCGGGAGCTGGAGCTGGCTGTGTTCTGGCGAGACCAGAGGGGTC TGTGTGCTCTCAAATTTCTGAAGTTGGAGGACTTCTTGGACAACGAGAGGCATGAGGTGCAGCTGGACAT GGAACCCCAGGGCTGCCTGGTGGCTGAGGTCACTTTCCGAAACCCCATCATCGAGCGGATCCCTAGGCTC CAAAGGCAGAAAAAAATCTTCTCCAAGCAGCAAGGGAAGGCATTTCAGCGAGCCAGACAGATGAACATAG ATGTGGCGACTTGGGTGCGGCTGCTCCGTAGACTCATCCCTAGTGCTGTGGCCACTGGCACCTTCAGTCC CAATGCATCTCCAGGTGCTGAGATCCGGCACACTGGAGACATATCCATGGAGAAATTGAATCTCGGTGCT GACTCAGACAGCTCGTCCCAAAAGAGCCCACCAGGGCTGCCCTCCACCTCATGCAGCCTGAGTTCTCCAA CCCATGAATCCACCACATCTCCAGAGCTGCCTTCAGAGACCCAGGAGACTCCAGGCCCTGGCCTGTGCAG CCCCTTGAGAAAGTCGCCCCTGACACTTGAGGACTTCAAGTTCCTGGCCGTGCTTGGCCGGGGTCACTTT GGAAAGGTGCTGCTGTCTGAATTCCGCTCCAGTGGGGAGCTCTTTGCCATCAAAGCCTTGAAGAAAGGTG ACATTGTAGCCCGAGATGAGGTTGAGAGCCTGATGTGTGAGAAGCGGATTTTGGCGGCCGTGACCAGGGC AGGACATCCCTTCCTGGTGAACCTTTTCGGCTGTTTCCAGACCCCAGAGCACGTGTGCTTTGTGATGGAG TACTCGGCGGGTGGAGACCTGATGCTGCACATTCATAGCGACGTGTTCTCAGAGCCTCGGGCTGTCTTCT ATTCGGCCTGTGTGGTGCTGGGACTGCAGTTCCTCCATGAACACAAGATTGTCTACAGGGACCTGAAGTT GGACAATTTGCTCCTGGATACTGAGGGCTACGTCAAGATCGCAGACTTTGGCCTCTGCAAGGAGGGGATG GGCTATGGGGACCGGACCAGCACGTTCTGCGGAACTCCGGAGTTCCTGGCGCCGGAAGTGCTCACAGACA CATCCTACACGCGAGCAGTGGACTGGTGGGGACTGGGCGTGCTGCTCTATGAGATGTTGGTTGGAGAGTC TCCGTTCCCTGGGGATGATGAGGAGGAGGTATTTGACAGCATTGTCAACGACGAAGTTCGCTATCCCCGC TTCCTGTCTGCAGAGGCCATCGGCATCATGAGAAGGCTACTGCGGAGGAACCCGGAGCGGAGGCTGGGGT CCACTGAGCGCGATGCAGAAGATGTGAAAAAACAGCCTTTCTTCCGGTCTCTGGGCTGGGATGTCCTGCT GGCCCGCCGCTTGCCTCCACCCTTCGTGCCTACACTTTCAGGGCGCACAGATGTCAGCAACTTCGATGAG GAGTTCACTGGGGAGGCCCCCACACTGAGTCCTCCCCGGGATGCACGGCCCCTCACAGCTGCGGAGCAGG CAGCCTTCCGGGATTTCGACTTTGTGGCCGGAGGCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211277 representing NM_177262
Red=Cloning site Green=Tags(s) MAGDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLKREIRKELKLKEGAENLRRATTDLGRSL APVELLLRGSARRLDLLHQQLQELHAHVVLPDPAAGSDATQSLAEGSPICSSTNLSRVAGLEKQLAIELK VKQGAENMIQTYSNGSSKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQALQAGELESQAAPDEAQGDPE LGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSGAKAPDRKAVSEAQEKLTESNQKLGLLRESLERRLGE LPADHPKGRLLREELTAASSSAFSAILPGPFPATHYSTLSKPAPLTGTLEVRVVGCKNLPETIPWSPPPS VGASGTPESRTPFLSRPARGLYSRSGSLSGRSSLRGEAENATEVSTVLKLDNTVVGQTAWKPCGPNAWDQ SFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPIIERIPRL QRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPSAVATGTFSPNASPGAEIRHTGDISMEKLNLGA DSDSSSQKSPPGLPSTSCSLSSPTHESTTSPELPSETQETPGPGLCSPLRKSPLTLEDFKFLAVLGRGHF GKVLLSEFRSSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTRAGHPFLVNLFGCFQTPEHVCFVME YSAGGDLMLHIHSDVFSEPRAVFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGM GYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPR FLSAEAIGIMRRLLRRNPERRLGSTERDAEDVKKQPFFRSLGWDVLLARRLPPPFVPTLSGRTDVSNFDE EFTGEAPTLSPPRDARPLTAAEQAAFRDFDFVAGGY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177262 |
| ORF Size | 2838 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177262.4, NP_796236.2 |
| RefSeq Size | 3436 bp |
| RefSeq ORF | 2841 bp |
| Locus ID | 320795 |
| UniProt ID | P70268 |
| Gene Summary | PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203356 | Pkn1 (untagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2, (10ug) |
CNY 6,936.00 |
|
| MR211277 | Pkn1 (Myc-DDK-tagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2 |
CNY 6,928.00 |
|
| MR211277L3 | Lenti ORF clone of Pkn1 (Myc-DDK-tagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2 |
CNY 10,260.00 |
|
| MR211277L4 | Lenti ORF clone of Pkn1 (mGFP-tagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2 |
CNY 10,260.00 |
