Txndc11 (BC029643) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2810408E11Rik; AI427833; EFP1; Txdc11
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211280 representing BC029643 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAATGCGGAGGCCGCGGCGGCGGCGGCGGGAACAACAGCGAGGACGCCGAGGACGAGGGTGGCG GCCCCAAGGGCTCAGGCTCCCTCAGCCCTGCCGGGGCGGCGGCGTCCTTGGAGGGCCGGATCCGCCGCGG CTTGCGCGGCGCCTCGCTTATGGCGCGCCAGCGGCCAGAGCTGCTGTGCGGGGCCGTGGCTCTCGGCTGC GCCCTGCTCTTCGCCCTCAAGTTCACTTGCAGTCGAGCAAAAGACGTGATAATACCAGCAAAGCCGCCTG TCAGCTTTTTCTCCTCGAGATCTCCGGTCCTTGACCTCTTCCAGGGGCAGCTGGACTATGCTGACCATGT CCGCCAGGATTCTGAGGTGGTGGTGCTGTTCTTCTATGCCCCGTGGTGTGGCCAGTCCATCGCTGCCAGG GCAGAAATTGAGCAGGCAGCCAGCCGGCTTTCTGATCAGGTGTTGTTTGTGGCAATAAACTGTTGGTGGA ACCAGGGGAAATGCAGAAAACAGAAGCATTTCTTCTATTTTCCTGTGATACATCTGTATCATCGGAGTTT TGGACCAATCGAATACAAAGGCCCCATGAGTGCTGTTTACATTGAGAAGTTTGTCCGCCGGGCGATGAAG CCGCTTCTCTACATCCCGTCTCAATCAGCATTGCTAGACTTTCTCTCCAGCTATGAGCCTGGAGTACTCG GATACTTTGAATTCAGCGGTTCACCCCAGCCTCCTGGTTACTTGACCTTCTTCACCTCAGCACTGCATTC GTTAAAGAAAGATTACCTAGGAACAGTGCGATTTGGGGTTATCACAGATAAGCACCTTGCGAGACTGGTA TCCTTAGTGCACTCTGGAAGTGTGTATTTACATAGACATTTCAACACATCACTTGTCTTCCCCAGGGAAG TCATGAACTTCACGGCTGAGAACATCTATAAGTGGGCCTCAGAAAACCAGGAGACCCTCTTCCGATGGCT ACAGCCTCATGGAGGCAAGAGTCTGCTGTTGAACAATGAGCTGAAGAAAGGACCAGCACTCTTCCTGTTC ATACCGTTTGATCCCCTAGCCGAGAGACATCCTCTCCTAGATGAGATCACCGAAGTGGCCTTGGAGTACA ATAACTGTCATGGGGACCAGGTGGTAGAGCGCCTCCTTCAGCACCTGCGGCGGGTAGAGGCACCTGTGCT CCAGTCCCTGGCACCAGAGCTGCCAGCTTCACTGCCTGATACTCAGCTGATGGCAGCATCCCCCTGCTGT AACACGGTGGTGCTGCCACAGGGGCCAGCCCTCTCCAGGACTCACAATGTCTGTGAGCTGTGTGTCAACC AGACAGTGGGTGGTACCAGGCCTAGCTCAGTGAGTGTGCCACAGTGCAGCTTCTTCGAGATGGCAGCCGC TCTGGATTCTTTCTACCTCAAGGAGCAGACTTTTTATCATGTGGTGTCGGGCAGCATAGAATGCAGCAAT TTTTTAACCTCCTATAGCCCTTTCAGCTACTACACTGCATGTTGCAGGACCATATCCCGGGGCATGGCAA GCTTTACTGGCTCTGAACAGAATGTCTTGACAGCCCCAGCCATCGAGTTCTCTTCCTTAGAGAAGAGCTG TGAGGCCACTGCCCCGAGCTCCATTCCTCACATTGAGGAGAACAGGTACCGCTTTCCTCAAGTGGGCCTC ACTAGCACAGCCTTCACGGGCCTGAGCTGCAGAACCAACAAGACCCTGAACATCTACCTTTTGGATTCCA ATTTATTTTGGTTATATGCAGAAAGACTGGGTGCCCCGAGCTCTGCTCCTGTGAAGGAGTTTGCCACAAT TGTTGATGTGAAAGAAGAATCTCACTATATCCTGGATCCCAAACAAGCCCTCATGAAGTTCACTCTAGAG TCTTTTATTCAAAACTTCAGTGTTCTCTACAGCCCCTTGAAAAGGCATCTCACTGGAAGTGATTCTGCTC AGTTTCCCACTCAGCATTTGATCACGGAAGTGACGACTGATACCTTCTGGGAAGTAACCCTTCGGAAACA GGACGTTCTGCTGCTCTATTATACACAGTGGTGTGGCTTTTGCCCGTCTCTCAATCACATCTTCATCCAG CTAGCTCGGCTCCTGCCAGAGGACACATTTACTGTGGCCAGGATCGATGTGTCTCAGAATGATCTTCCTT GGGAATTCATGGTTGACCGTCTTCCTACCGTTTTGTTTTTTCCCTGCAATAGAAAGGACCTAAGTGTGAA ATACCCTGGAGACCTCCCTATCACCCTCCCGAATCTGCTGCGGTTCATTCTGCATCACTCAGACGCTGCC TCTGCCCCCCAGGACCCTGGCATCAGCCCTCCTACCCAAGACTGTGTCCAGAGCAAGGCAGTGCTGCAGC GCGAACACATCTCTCACGTGGAGAATGCGATGCAGAAGCTGAGATCTGAGATGAGCAGCCTACGCCGTAC ACAGGAGCAGGTGGAGGGCAGGCTCCTTAGTGCCCGCAGGGATGGGCACCGCCTGCTGCGCCGGCAGCGG ACCCTGGAGCAGCAGCACAGGCTACTCCGGCGCCACAGCCAGAAACTGCAGGCTCTGTATTTAAAGAAGG CTCGAGAGCTCCAAGAGCTGGCTAGAGCCTCAGGAACCCCCCTCCCCGAGCACACATGGCTCAAGATCCT TGTGGCCACTATGGAGAGGGAGCTGGAAGGCCAAGGTGGGGCTAAGGAACCAGCCCCTCTGGGAAAGGCT CGACCGAACCACTCCAAGTCCATGGGTACAACTCAGTTACCTGGTGACACCCCTCCGCCCTCCACCACCA GCTCCACTTTGGCTTCAGAGACGAAGCATGAGAATAGGACAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211280 representing BC029643 Red=Cloning site Green=Tags(s) MSECGGRGGGGGNNSEDAEDEGGGPKGSGSLSPAGAAASLEGRIRRGLRGASLMARQRPELLCGAVALGC ALLFALKFTCSRAKDVIIPAKPPVSFFSSRSPVLDLFQGQLDYADHVRQDSEVVVLFFYAPWCGQSIAAR AEIEQAASRLSDQVLFVAINCWWNQGKCRKQKHFFYFPVIHLYHRSFGPIEYKGPMSAVYIEKFVRRAMK PLLYIPSQSALLDFLSSYEPGVLGYFEFSGSPQPPGYLTFFTSALHSLKKDYLGTVRFGVITDKHLARLV SLVHSGSVYLHRHFNTSLVFPREVMNFTAENIYKWASENQETLFRWLQPHGGKSLLLNNELKKGPALFLF IPFDPLAERHPLLDEITEVALEYNNCHGDQVVERLLQHLRRVEAPVLQSLAPELPASLPDTQLMAASPCC NTVVLPQGPALSRTHNVCELCVNQTVGGTRPSSVSVPQCSFFEMAAALDSFYLKEQTFYHVVSGSIECSN FLTSYSPFSYYTACCRTISRGMASFTGSEQNVLTAPAIEFSSLEKSCEATAPSSIPHIEENRYRFPQVGL TSTAFTGLSCRTNKTLNIYLLDSNLFWLYAERLGAPSSAPVKEFATIVDVKEESHYILDPKQALMKFTLE SFIQNFSVLYSPLKRHLTGSDSAQFPTQHLITEVTTDTFWEVTLRKQDVLLLYYTQWCGFCPSLNHIFIQ LARLLPEDTFTVARIDVSQNDLPWEFMVDRLPTVLFFPCNRKDLSVKYPGDLPITLPNLLRFILHHSDAA SAPQDPGISPPTQDCVQSKAVLQREHISHVENAMQKLRSEMSSLRRTQEQVEGRLLSARRDGHRLLRRQR TLEQQHRLLRRHSQKLQALYLKKARELQELARASGTPLPEHTWLKILVATMERELEGQGGAKEPAPLGKA RPNHSKSMGTTQLPGDTPPPSTTSSTLASETKHENRTD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC029643
ORF Size	2846 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029643, AAH29643
RefSeq Size	3049 bp
RefSeq ORF	2846 bp
Locus ID	106200
Gene Summary	May act as a redox regulator involved in DUOX proteins folding. The interaction with DUOX1 and DUOX2 suggest that it belongs to a multiprotein complex constituting the thyroid H(2)O(2) generating system. It is however not sufficient to assist DUOX1 and DUOX2 in H(2)O(2) generation (By similarity).[UniProtKB/Swiss-Prot Function]

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