Aars (BC033273) Mouse Tagged ORF Clone
CAT#: MG211342
- TrueORF®
Aars (tGFP-tagged) - Mouse alanyl-tRNA synthetase (cDNA clone MGC:25868 IMAGE:4196789)
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CNY 7,890.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MGC37368 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211342 representing BC033273
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCCACTTTAACAGCACGAGAGATCCGAGAGCGGTTTATAAATTTCTTCAGACGAAATGAGCACA CCTATGTCCACTCTTCCGCCACCATCCCTCTGGATGACCCCACTCTGCTCTTCGCCAACGCTGGCATGAA CCAGTTCAAACCCATCTTCCTAAACACAATCGACCCATCTCACCCTATGGCGAAGCTGAGCAGAGCTGCC AATACCCAGAAATGCATCCGAGCTGGGGGCAAGCACAACGACCTGGATGACGTGGGCAAGGATGTCTACC ACCACACTTTCTTTGAGATGCTGGGCTCCTGGTCTTTTGGGGACTATTTCAAGGAATTGGCATGTAAGAT GGCCCTGGAACTTCTTACCCAGGAGTTTGGTATTCCAGTTGAAAGACTCTATGTCACTTACTTTGGTGGT GATGAGGCGGCCGGCCTAGAGCCAGATCTGGAGTGCAGACAGATTTGGCAAAATTTAGGACTGGATGAAG CCAGAATCCTCCCTGGCAACATGAAGGACAACTTCTGGGAGATGGGTGACACAGGCCCCTGTGGTCCCTG CAGCGAGATCCACTATGACCGCATTGGTGGCAGGGACGCCGCACACCTTGTCAACCAGGATGACCCCAAT GTGCTGGAGATCTGGAACCTCGTGTTCATCCAGTATAACAGGGAGTCGGATGGTGTTCTGAAACCTCTTC CCAAGAAAAGCATCGACACAGGGATGGGCCTAGAGAGGCTGGTGTCTGTGCTGCAGAACAAGATGTCCAA CTACGACACCGACCTTTTCATGCCTTACTTCGAAGCCATTCAGAAGGGCACCGGCGCCCGGCCGTATACT GGGAAGGTTGGTGCTGAGGATGCAGACGGAATCGACATGGCCTACCGGGTTCTGGCTGACCACGCCCGGA CCATCACTGTGGCGCTGGCTGATGGCGGCCGGCCTGACAACACAGGACGGGGGTACGTGCTGAGACGGAT CCTTCGCCGAGCTGTTCGGTATTCCCATGAGAAACTGAATGCCAGCAGGGGTTTCTTCGCTACATTGGTT GATGTTGTCGTTCAATCCCTGGGAGACGCATTTCCTGAGCTGAAGAAGGACCCAGAGATGGTGAAGGACA TCATTAATGAAGAAGAGGTGCAGTTTCTCAAGACTCTCAGCCGAGGGCGGCGCATCCTGGACCGAAAAAT TCAGAGCTTAGGAGACTGCAAAACCATCCCAGGTGACACCGCTTGGCTCCTCTATGATACTTATGGATTC CCAGTGGACCTCACTGGATTGATTGCTGAAGAGAAGGGCCTGGTGGTAGATATGAATGGCTTTGAGGAAG AGAGGAGACTGGCCCAGCTGAAGTCACAGGGCAAGGGAGCAGGGGACGAAGACCTCATCATGCTGGACAT TTACGCTATTGAAGAGCTCCGGGCAAAGGGTTTGGAGGCCACAGATGATTCTCCAAAGTATAACTACCAA TCAGACTCCAGTGGTAGCTACGTGTTTGAGTGTACAGTGGCTACCGTGCTTGCTCTGCGTCGGGAAAAGA TGTTTGTGGACGAGGTGGTCACTGGCCAGGAGTGTGGAGTGGTGCTGGACAAGACCTGTTTCTATGCGGA GCAAGGAGGGCAGATCTACGATGAAGGCTACTTGGTGAAGGTCGACGACAGCAGTGAGGACAAAACCGAG TTTACAGTGAAGAACGCTCAGGTCCGAGGCGGGTACGTGCTGCACATAGGAACGATATATGGTAACCTGA AAGTGGGGGATCAAGTCCGGCTGTTTATTGATGAGCCCCGGCGGAGACCCGTCATGAGCAACCACACAGC TACACACATCCTCAACTTCGCCTTGCGCTCGGTGCTTGGGGAAGCGGACCAGAAAGGTTCACTGGTTGCT CCTGACCGCCTTCGGTTTGACTTCACTGCCAAGGGAGCCATGTCCACCCAGCAAATCAAGAAGGCTGAAG AGATTGTCAATGGCATGATTGAGGCAGCTAAGCCTGTCTACACCCAGGATTGTCCCCTGGCAGCAGCAAA AGCCATCCAGGGCCTTCGAGCTGTGTTTGATGAGACCTATCCTGACCCCGTTCGAGTCGTCTCCATTGGG GTCCCCGTGTCAGAGCTGCTGGATGACCCGTGTGGGCCTGCTGGCTCCCTTACTTCTGTTGAGTTCTGTG GAGGAACACATCTACGGAATTCTAGCCACGCAGGCGCTTTTGTGATAGTGACAGAAGAAGCTATTGCCAA GGGTATCCGGAGGATTGTTGCTGTCACAGGTGCTGAAGCCCAGAAGGCTCTCAGGAAGTCAGAAACCTTG AAGAAATCTCTCTCTGCCATGGAGGCCAAAGTGAAGGCCCAGACTGCACCCAACAAGGACGTGCAGAGGG AAATTGCTGACCTCGGTGAGGCCCTGGCCACTGCAGTCATCCCCCAGTGGCAGAAAGATGAACAACGAGA GACTCTGAAGTCCCTGAAGAAAGTCATGGATGACCTAGACCGGGCCAGCAAAGCCGATGTGCAGAAGCGA GTGTTAGAGAAGACAAAGCAGCTGATTGACAGCAACCCCAACCAGCCGCTGGTCATCCTGGAGATGGAGA GTGGAGCATCGGCCAAGGCCCTGAATGAAGCGTTGAAGCTTTTCAAGACGCATTCTCCTCAGACATCTGC CATGCTGTTCACGGTGGACAATGAGGCTGGCAAGATCACGTGCTTGTGCCAGGTTCCCCAGAATGCAGCC AACCGGGGACTGAAAGCCAGCGAGTGGGTCCAGCAGGTTTCAGGCCTGATGGATGGTAAAGGCGGTGGCA AGGACATGTCCGCCCAGGCCACAGGCAAGAACGTGGGCTGCCTTCAGGAAGCACTGCAGCTGGCTACCTC CTTTGCCCAGCTCCGTCTGGGAGATGTGAAGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211342 representing BC033273
Red=Cloning site Green=Tags(s) MDATLTAREIRERFINFFRRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAA NTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGG DEAAGLEPDLECRQIWQNLGLDEARILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPN VLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFMPYFEAIQKGTGARPYT GKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLV DVVVQSLGDAFPELKKDPEMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCKTIPGDTAWLLYDTYGF PVDLTGLIAEEKGLVVDMNGFEEERRLAQLKSQGKGAGDEDLIMLDIYAIEELRAKGLEATDDSPKYNYQ SDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTE FTVKNAQVRGGYVLHIGTIYGNLKVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGEADQKGSLVA PDRLRFDFTAKGAMSTQQIKKAEEIVNGMIEAAKPVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIG VPVSELLDDPCGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETL KKSLSAMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKR VLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAA NRGLKASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC033273 |
ORF Size | 2906 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC033273, AAH33273 |
RefSeq Size | 4891 bp |
RefSeq ORF | 2906 bp |
Locus ID | 234734 |
Gene Summary | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala) (PubMed:16906134, PubMed:20010690, PubMed:25422440, PubMed:27622773). Also edits incorrectly charged tRNA(Ala) via its editing domain (PubMed:16906134, PubMed:20010690, PubMed:25422440, PubMed:29769718).[UniProtKB/Swiss-Prot Function] |
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