Atp2a3 (BC026147) Mouse Tagged ORF Clone

CAT#: MG211414

  • TrueORF®

Atp2a3 (tGFP-tagged) - Mouse ATPase, Ca++ transporting, ubiquitous (cDNA clone MGC:13968 IMAGE:4191458)



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Mouse Protein / Lysate Request

CNY 11,020.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms SERCA3b, Serca3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211414 representing BC026147
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGCGCACCTGCTCTCCGCTGCCGACGTGCTGCGTCGCTTCTCGGTGACAGCCGAGGGCGGCT
TGAGCCTGGAGCAGGTGACCGACGCGCGGGAGCGTTACGGCCCCAACGAGCTCCCGACCGAGGAAGGGAA
ATCCCTGTGGGAGCTGGTGGTGGAGCAGTTTGAGGACCTCTTGGTGCGCATCCTGCTGCTGGCAGCTCTG
GTTTCCTTTGTCCTGGCCTGGTTCGAGGAGGGTGAGGAGACCACAACGGCCTTCGTGGAGCCCCTGGTCA
TCATGCTAATTCTCGTGGCCAATGCGATCGTGGGAGTGTGGCAGGAACGCAACGCAGAGAGTGCAATTGA
GGCCTTGAAGGAGTATGAGCCTGAGATGGGTAAGGTGATCCGGTCTGACCGCAAGGGTGTACAGAGGATC
CGAGCCAGGGACATCGTCCCTGGGGACATTGTGGAAGTGGCAGTGGGAGACAAGGTACCCGCTGACCTCC
GTCTCATCGAGATCAAGTCCACCACACTTCGAGTGGACCAGTCCATCCTGACAGGGGAATCCGTGTCTGT
GACGAAGCACACAGATGCCATTCCAGACCCCCGAGCCGTGAACCAGGACAAGAAGAACATGCTATTTTCT
GGCACCAATATTGCATCAGGCAAAGCCCTGGGTGTGGCTGTGGCTACAGGCCTGCAGACAGAGCTGGGCA
AGATTCGGAGCCAGATGGCGGCTGTGGAGCCTGAGCGGACGCCATTGCAGCGCAAGCTGGATGAATTTGG
GAGGCAGCTGTCCCATGCCATCTCTGTGATCTGTGTGGCTGTGTGGGTCATCAACATCGGCCACTTTGCT
GACCCGGCCCACGGTGGATCCTGGCTCCGTGGTGCAGTCTACTACTTCAAGATCGCTGTGGCCCTGGCTG
TGGCTGCTATCCCCGAGGGTCTCCCAGCAGTCATCACTACATGCCTGGCCCTGGGCACAAGACGTATGGC
ACGTAAGAATGCCATTGTGCGGAGCCTGCCTTCTGTGGAGACGCTGGGCTGCACCTCGGTCATCTGCTCT
GACAAGACGGGGACGCTCACCACCAATCAGATGTCTGTCTGCAGGATGTTCGTGGTGGCTGAAGCAGAGG
CAGGCACCTGTCGTTTGCATGAATTCACCATTTCGGGTACCACGTACACCCCAGAGGGCGAAGTGAGGCA
AGGGGAGCAGCCTGTGCGCTGTGGGCAGTTTGATGGGCTAGTGGAGCTCGCCACCATCTGTGCCCTCTGC
AATGATTCTGCGCTGGACTACAATGAGGCCAAGGGTGTGTATGAGAAGGTAGGAGAGGCCACAGAGACCG
CCCTGACTTGCCTGGTAGAGAAGATGAATGTGTTTGACACAGACCTGAAAGGACTGTCGCGGGTGGAGCG
TGCTGGCGCCTGCAACTCAGTCATCAAGCAACTCATGCGGAAAGAGTTCACCCTGGAGTTCTCCCGGGAC
CGGAAGTCTATGTCCGTGTACTGCACACCTACTCGTGCTGACCCCAAGGTCCAGGGCAGCAAGATGTTTG
TGAAGGGAGCTCCTGAGAGTGTAATTGAGCGCTGTAGCTCAGTCCGAGTGGGCAGCCGAACAGCACCCCT
GAGCACCACTTCCAGAGAGCATATCCTGGCCAAGATCCGGGACTGGGGCTCAGGCTCTGACACACTGCGC
TGCTTAGCCTTGGCCACCAGGGACACACCCCCAAGGAAGGAGGACATGCATCTAGATGACTGCAGCCGGT
TTGTACAGTACGAGACAGACCTGACCTTCGTGGGCTGCGTGGGCATGTTAGACCCACCGAGACCAGAGGT
GGCTGCTTGTATCACACGCTGTTCCCGGGCTGGCATTCGAGTGGTCATGATCACAGGGGACAACAAAGGG
ACAGCTGTGGCCATCTGCCGCCGACTTGGCATCTTTGGGGACACGGAGGATGTGTTGGGCAAGGCCTACA
CGGGCCGTGAGTTTGATGACCTCAGCCCAGAGCAGCAGCGCCAGGCTTGTCGCACTGCCCGGTGCTTTGC
CCGAGTGGAACCCGCACATAAGTCTCGAATCGTGGAGAACCTACAGTCCTTTAATGAGATCACTGCCATG
ACTGGCGATGGGGTGAATGATGCACCAGCCCTGAAGAAGGCAGAGATCGGCATCGCCATGGGCTCAGGCA
CTGCTGTGGCCAAGTCAGCAGCAGAGATGGTGCTGTCCGATGACAACTTTGCCTCCATTGTGGCTGCAGT
AGAAGAGGGCAGGGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCCTCCAATGTTGGC
GAGGTTGTCTGCATCTTCCTCACAGCAATTCTGGGCCTGCCCGAAGCTCTGATCCCTGTGCAGCTGCTCT
GGGTGAACCTGGTAACAGATGGCTTACCGGCCACAGCCCTTGGCTTCAACCCACCAGATCTGGATATCAT
GGAGAAGCCGCCTCGGAATCCCCGTGAGGCCCTCATCAGTGGCTGGCTTTTTTTCCGCTATTTGGCTATT
GGAGTGTATGTAGGCCTGGCTACAGTGGCTGCCGCCACCTGGTGGTTCCTGTATGACACCGAGGGACCTC
AAGTCACCTTCTATCAGCTGAGGAACTTCCTGAAGTGCTCTGAAGACAATCCACTGTTTGCTGGCATTGA
CTGCAAGGTTTTTGAGTCACGCTTCCCCACAACCATGGCCTTATCTGTGCTTGTGACCATTGAAATGTGC
AACGCCCTCAACAGCGTCTCTGAGAACCAGTCACTGCTGCGCATGCCACCCTGGCTGAACCCTTGGCTGC
TGGGGGCTGTGGTCATGTCCATGGCTCTGCATTTTCTTATCCTCCTGGTGCCACCTCTGCCTCTCATTTT
TCAGGTGACCCCACTGAGTGGTCGGCAGTGGGGGGTGGTGCTTCAGATGTCTCTGCCTGTCATCCTGCTC
GATGAGGCCCTCAAGTATCTGTCCAGGAATCACATGGATGAAAAAAAGGACCTGAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211414 representing BC026147
Red=Cloning site Green=Tags(s)

MEEAHLLSAADVLRRFSVTAEGGLSLEQVTDARERYGPNELPTEEGKSLWELVVEQFEDLLVRILLLAAL
VSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQERNAESAIEALKEYEPEMGKVIRSDRKGVQRI
RARDIVPGDIVEVAVGDKVPADLRLIEIKSTTLRVDQSILTGESVSVTKHTDAIPDPRAVNQDKKNMLFS
GTNIASGKALGVAVATGLQTELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFA
DPAHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICS
DKTGTLTTNQMSVCRMFVVAEAEAGTCRLHEFTISGTTYTPEGEVRQGEQPVRCGQFDGLVELATICALC
NDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLKGLSRVERAGACNSVIKQLMRKEFTLEFSRD
RKSMSVYCTPTRADPKVQGSKMFVKGAPESVIERCSSVRVGSRTAPLSTTSREHILAKIRDWGSGSDTLR
CLALATRDTPPRKEDMHLDDCSRFVQYETDLTFVGCVGMLDPPRPEVAACITRCSRAGIRVVMITGDNKG
TAVAICRRLGIFGDTEDVLGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITAM
TGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYNNMKQFIRYLISSNVG
EVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKPPRNPREALISGWLFFRYLAI
GVYVGLATVAAATWWFLYDTEGPQVTFYQLRNFLKCSEDNPLFAGIDCKVFESRFPTTMALSVLVTIEMC
NALNSVSENQSLLRMPPWLNPWLLGAVVMSMALHFLILLVPPLPLIFQVTPLSGRQWGVVLQMSLPVILL
DEALKYLSRNHMDEKKDLK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC026147
ORF Size 2999 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC026147, AAH26147
RefSeq Size 4489 bp
RefSeq ORF 2999 bp
Locus ID 53313
Gene Summary This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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