Tnk2 (BC052421) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Ack, Cdgip, ACK1, MGC37479
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211437 representing BC052421 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGGAGGAGGGAACGGGCTGGCTGTTGGAGCTGCTGTCCGAGGTGCAGCTACAACAGTATTTCC TGAGGCTTCGAGATGACCTCAACATTACCCGCCTATCTCATTTTGAATATGTCAAAAATGAAGACCTGGA AAAGATTGGCATGGGCCGGCCTGGCCAGAGGCGGCTGTGGGAGGCCGTGAAGAGGAGGAAGGCCATGTGC AAACGCAAGTCATGGATGAGCAAGGTGTTCAGTGGAAAGCGGTTGGAGGCCGAGTTCCCTTCCCAGCACT CTCAGAGCACCTTCCGGAAGCCCTCCCCCACCCCAGGGAGCCTGCCAGGGGAGGGGACCCTGCAGAGCCT CACCTGCCTCATTGGGGAGAAAGACCTACGCCTGCTGGAGAAGCTGGGGGATGGCTCCTTTGGCGTGGTG CGCAGGGGTGAATGGGACGCCCCCGCAGGGAAGACGGTGAGTGTGGCCGTCAAGTGCCTGAAGCCTGACG TGCTGAGCCAGCCAGAGGCCATGGACGACTTCATCCGGGAGGTCAATGCCATGCATTCACTAGACCACCG AAACCTCATTCGCTTGTATGGTGTGGTGCTCACACTACCCATGAAGATGGTGACAGAGCTGGCACCTCTG GGATCTTTGTTGGACCGCCTACGTAAACACCAAGGTCATTTCCTCTTGGGGACGCTGAGTCGCTACGCTG TGCAGGTGGCTGAGGGTATGGCCTACCTGGAGTCCAAGCGCTTCATTCACCGGGATCTGGCTGCTCGAAA CCTGCTTTTGGCTACCCGGGACCTGGTCAAGATTGGGGACTTCGGACTGATGCGAGCTCTGCCCCAGAAT GATGACCACTATGTCATGCAAGAACACCGCAAGGTGCCCTTTGCCTGGTGTGCCCCTGAGAGCCTGAAGA CACGGACTTTCTCCCATGCCAGTGACACCTGGATGTTTGGGGTCACACTGTGGGAGATGTTCACCTATGG CCAGGAGCCCTGGATTGGCCTCAATGGCAGCCAGATCCTGCATAAGATCGACAAGGAAGGGGAGCGCCTG CCCCGGCCGGAGGACTGCCCTCAAGACATCTACAATGTCATGGTCCAGTGCTGGGCCCACAAGCCAGAGG ACAGACCCACATTTGTGGCTCTTCGGGACTTCCTGCTGGAGGCTCAGCCCACTGACATGCGGGCTCTTCA GGACTTTGAGGAGCCAGATAAACTGCACATCCAGATGAATGACGTCATCACTGTCATCGAGGGAAGGGCT GAGAACTACTGGTGGCGTGGGCAGAATACGCGGACCCTGTGTGTAGGACCCTTCCCTCGAAATGTCGTGA CCTCCGTGGCTGGCCTGTCAGCCCAGGACATCAGCCAGCCTCTACAGAATAGCTTCATTCACACAGGACA TGGTGACAGTGACCCCCGCCACTGCTGGGGGTTCCCTGACAGGATCGATGAACTGTACCTGGGAAACCCC ATGGACCCTCCTGACCTGCTGAGTGTGGAACTGAGCACCTCCCGACCCACCCAGCACCTAGGACGGGTGA AAAAGCCAACATACGACCCTGTGAGTGAGGACCCAGACCCCCTGTCCAGCGACTTCAAGAGGCTTGGCCT GAGGAAGCCAGCCCTGCCTCGAGGGCTGTGGCTGGCAAAGCCCTCAGCCCGAGTGCCAGGCACCAAGGCA GACCGCAGCAGTGGGGGTGAGGTCACACTCATCGACTTCGGTGAGGAGCCTGTGGTCCCAACCCCTCGGC CCTGTGCACCCTCCTTGGCACAGTTGGCCATGGATGCCTGCTCCTTGCTGGACAAGACACCACCACAGAG CCCCACACGGGCACTGCCACGACCTTTACACCCCACCCCTGTGGTAGACTGGGACGCGCGTCCGCTGCCC CCGCCCCCTGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTGAGGTCTGCTCCATCAACAGCACGC TAGTAGGTGCAGGCCTCCCTGCTGGGCCTAGCCAAGGCGAGACCAATTACGCCTTTGTACCTGAGCAGGC GCAGATGCCCCCTGCCCTGGAGGATAACCTGTTCCTTCCACCCCAGGGCGGAGGCAAGCCGCCCAGCTCA GTGCAGACTGCAGAGATTTTCCAGGCACTGCAGCAGGAGTGTATGCGGCAGCTACAGGTCCCCACTGGCC AGCTGACCCCCTCCCCGACCCCAGGAGGTGATGACAAGCCCCAGGTGCCACCCCGGGTACCTATTCCCCC TCGGCCCACGCGTCCACGTGTGGAGCTATCTCCAGCTCCCTCGGGTGAGGAAGAGACAAGCCGGTGGCCT GGACCTGCCTCGCCCCCCCGAGTGCCTCCCCGGGAACCTCTGTCTCCTCAAGGTTCAAGGACCCCAAGCC CCCTAGTGCCACCTGGCAGCTCTCCACTACCGCATCGGCTCTCTAGCTCACCTGGAAAGACCATGCCCAC CACTCAAAGCTTTGCCTCAGACCCTAAGTATGCCACTCCACAAGTGATCCAGGCTCCTGGCCCACGGGCA GGCCCCTGCATCCTGCCCATTGTCCGCGATGGCAGGAAGGTCAGCAGCACTCATTACTACCTGCTGCCTG AGCGCCCTCCTTACCTGGAACGCTATCAGCGCTTCCTGCGGGAGGCCCAGAGCCCGGAAGAGCCGGCCGC CCTGCCTGTGCCCCCGCTGTTGCCCCCGCCCAGTACTCCAGCCCCTGCTGCCCCCACTGCCACCGTCAGA CCTATGCCTCAGGCCGCCCCAGACCCAAAGGCCAACTTCTCCACCAATAACAGCAACCCAGGGGCACGGC CACCATCCCTGAGGGCCACGGCTCGGCTGCCACAGAGGGGCTGCCCAGGGGACGGGCAAGAGGCTGCTCG GCCAGCAGACAAGGTCCAGATGGTGGAGCAGCTCTTTGGGCTGGGTCTTCGGCCACGGGTGGAGTGCCAC AAGGTCCTAGAGATGTTCGACTGGAACCTAGAGCAAGCCGGCTGTCACCTTCTGGGCTCCTGTGGCCCTG CTCATCACAAACGC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211437 representing BC052421 Red=Cloning site Green=Tags(s) MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNITRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMC KRKSWMSKVFSGKRLEAEFPSQHSQSTFRKPSPTPGSLPGEGTLQSLTCLIGEKDLRLLEKLGDGSFGVV RRGEWDAPAGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTLPMKMVTELAPL GSLLDRLRKHQGHFLLGTLSRYAVQVAEGMAYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQN DDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERL PRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRA ENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNP MDPPDLLSVELSTSRPTQHLGRVKKPTYDPVSEDPDPLSSDFKRLGLRKPALPRGLWLAKPSARVPGTKA DRSSGGEVTLIDFGEEPVVPTPRPCAPSLAQLAMDACSLLDKTPPQSPTRALPRPLHPTPVVDWDARPLP PPPAYDDVAQDEDDFEVCSINSTLVGAGLPAGPSQGETNYAFVPEQAQMPPALEDNLFLPPQGGGKPPSS VQTAEIFQALQQECMRQLQVPTGQLTPSPTPGGDDKPQVPPRVPIPPRPTRPRVELSPAPSGEEETSRWP GPASPPRVPPREPLSPQGSRTPSPLVPPGSSPLPHRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRA GPCILPIVRDGRKVSSTHYYLLPERPPYLERYQRFLREAQSPEEPAALPVPPLLPPPSTPAPAAPTATVR PMPQAAPDPKANFSTNNSNPGARPPSLRATARLPQRGCPGDGQEAARPADKVQMVEQLFGLGLRPRVECH KVLEMFDWNLEQAGCHLLGSCGPAHHKR SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC052421
ORF Size	3024 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC052421, AAH52421
RefSeq Size	4334 bp
RefSeq ORF	3026 bp
Locus ID	51789
Gene Summary	Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.[UniProtKB/Swiss-Prot Function]

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